Genetic analysis of environment-sensitive genic male sterile rice under US environments
Two line hybrid rice ( Oryza sativa L.) breeding uses environment-sensitive genic male sterile (EGMS) lines to produce sterile or fertile pollen depending on daylength and/or temperature. There is limited information on the performance and genetic control of EGMS lines under U.S. environments. There...
Gespeichert in:
| Veröffentlicht in: | Euphytica 2019-02, Vol.215 (2), p.1-8, Article 39 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 8 |
|---|---|
| container_issue | 2 |
| container_start_page | 1 |
| container_title | Euphytica |
| container_volume | 215 |
| creator | De Guzman, Christian T. Linscombe, Steven D. Oard, James H. |
| description | Two line hybrid rice (
Oryza sativa
L.) breeding uses environment-sensitive genic male sterile (EGMS) lines to produce sterile or fertile pollen depending on daylength and/or temperature. There is limited information on the performance and genetic control of EGMS lines under U.S. environments. Therefore, genetic characterization of two F
2
and four BC
1
F
2
populations derived from EGMS line 2009S was conducted under Louisiana field conditions. Chi squared analyses in the F
2
and BC
1
F
2
populations indicated that pollen sterility under high temperature and long daylength field conditions was controlled by a single recessive gene. Sequence comparisons at locus LOC_Os02g12290 between 2009S, CL161 (USDA-AMS
2002
) and published sequences of G63S and Nipponbare revealed a single nucleotide polymorphism (SNP) that has been detected previously in several EGMS lines. Due to high GC content, a CEL1 nuclease assay was used to detect SNPs associated with pollen sterility in 177 F
2
and 59 BC
1
F
2
sampled individuals. A high percentage of lines (90–100%) across all segregating populations were identified correctly as pollen sterile using the CEL 1 assay. Results from this study suggest that single-gene control of pollen sterility in EGMS line 2009S will be compatible with a two-line system for U.S. hybrid rice development. |
| doi_str_mv | 10.1007/s10681-019-2363-z |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_journals_2175286509</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A714073723</galeid><sourcerecordid>A714073723</sourcerecordid><originalsourceid>FETCH-LOGICAL-c355t-91f83bc42d16d5576027990d27f689034c4c88e84aeba3c03a9b2ca81644edbc3</originalsourceid><addsrcrecordid>eNp1kMFKAzEQhoMoWKsP4G3B89ZJskl2j6VoFQoetHgM2exsSWmzNdkW2qc3ZQW9yBzmMP83zHyE3FOYUAD1GCnIkuZAq5xxyfPTBRlRoXguQMIlGQHQIk24vCY3Ma4BoFICRuRzjh57ZzPjzeYYXcy6NkN_cKHzW_R9HtFH17sDZiv0Kbc1G8xij8GlHpzFbO8bDNny_S8Wb8lVazYR7376mCyfnz5mL_nibf46my5yy4Xo84q2Ja9twRoqGyGUBKaqChqmWllWwAtb2LLEsjBYG26Bm6pm1pRUFgU2teVj8jDs3YXua4-x1-tuH9IvUTOqBCulgCqlJkNqla7XzrddH4xN1eDW2c5jm77RU0ULUFwlTWNCB8CGLsaArd4FtzXhqCnos3A9CNdJuD4L16fEsIGJKetXGH5P-R_6Bq4fg-s</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2175286509</pqid></control><display><type>article</type><title>Genetic analysis of environment-sensitive genic male sterile rice under US environments</title><source>SpringerNature Journals</source><creator>De Guzman, Christian T. ; Linscombe, Steven D. ; Oard, James H.</creator><creatorcontrib>De Guzman, Christian T. ; Linscombe, Steven D. ; Oard, James H.</creatorcontrib><description>Two line hybrid rice (
Oryza sativa
L.) breeding uses environment-sensitive genic male sterile (EGMS) lines to produce sterile or fertile pollen depending on daylength and/or temperature. There is limited information on the performance and genetic control of EGMS lines under U.S. environments. Therefore, genetic characterization of two F
2
and four BC
1
F
2
populations derived from EGMS line 2009S was conducted under Louisiana field conditions. Chi squared analyses in the F
2
and BC
1
F
2
populations indicated that pollen sterility under high temperature and long daylength field conditions was controlled by a single recessive gene. Sequence comparisons at locus LOC_Os02g12290 between 2009S, CL161 (USDA-AMS
2002
) and published sequences of G63S and Nipponbare revealed a single nucleotide polymorphism (SNP) that has been detected previously in several EGMS lines. Due to high GC content, a CEL1 nuclease assay was used to detect SNPs associated with pollen sterility in 177 F
2
and 59 BC
1
F
2
sampled individuals. A high percentage of lines (90–100%) across all segregating populations were identified correctly as pollen sterile using the CEL 1 assay. Results from this study suggest that single-gene control of pollen sterility in EGMS line 2009S will be compatible with a two-line system for U.S. hybrid rice development.</description><identifier>ISSN: 0014-2336</identifier><identifier>EISSN: 1573-5060</identifier><identifier>DOI: 10.1007/s10681-019-2363-z</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Analysis ; Biomedical and Life Sciences ; Biotechnology ; Daylength ; Genetic analysis ; Genetic aspects ; Genetic control ; High temperature ; Hybrid systems ; Life Sciences ; Nuclease ; Oryza sativa ; Plant breeding ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Plant Sciences ; Pollen ; Polymorphism ; Populations ; Single nucleotide polymorphisms ; Single-nucleotide polymorphism ; Sterility</subject><ispartof>Euphytica, 2019-02, Vol.215 (2), p.1-8, Article 39</ispartof><rights>Springer Nature B.V. 2019</rights><rights>COPYRIGHT 2019 Springer</rights><rights>Euphytica is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c355t-91f83bc42d16d5576027990d27f689034c4c88e84aeba3c03a9b2ca81644edbc3</citedby><cites>FETCH-LOGICAL-c355t-91f83bc42d16d5576027990d27f689034c4c88e84aeba3c03a9b2ca81644edbc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10681-019-2363-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10681-019-2363-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>De Guzman, Christian T.</creatorcontrib><creatorcontrib>Linscombe, Steven D.</creatorcontrib><creatorcontrib>Oard, James H.</creatorcontrib><title>Genetic analysis of environment-sensitive genic male sterile rice under US environments</title><title>Euphytica</title><addtitle>Euphytica</addtitle><description>Two line hybrid rice (
Oryza sativa
L.) breeding uses environment-sensitive genic male sterile (EGMS) lines to produce sterile or fertile pollen depending on daylength and/or temperature. There is limited information on the performance and genetic control of EGMS lines under U.S. environments. Therefore, genetic characterization of two F
2
and four BC
1
F
2
populations derived from EGMS line 2009S was conducted under Louisiana field conditions. Chi squared analyses in the F
2
and BC
1
F
2
populations indicated that pollen sterility under high temperature and long daylength field conditions was controlled by a single recessive gene. Sequence comparisons at locus LOC_Os02g12290 between 2009S, CL161 (USDA-AMS
2002
) and published sequences of G63S and Nipponbare revealed a single nucleotide polymorphism (SNP) that has been detected previously in several EGMS lines. Due to high GC content, a CEL1 nuclease assay was used to detect SNPs associated with pollen sterility in 177 F
2
and 59 BC
1
F
2
sampled individuals. A high percentage of lines (90–100%) across all segregating populations were identified correctly as pollen sterile using the CEL 1 assay. Results from this study suggest that single-gene control of pollen sterility in EGMS line 2009S will be compatible with a two-line system for U.S. hybrid rice development.</description><subject>Analysis</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Daylength</subject><subject>Genetic analysis</subject><subject>Genetic aspects</subject><subject>Genetic control</subject><subject>High temperature</subject><subject>Hybrid systems</subject><subject>Life Sciences</subject><subject>Nuclease</subject><subject>Oryza sativa</subject><subject>Plant breeding</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Pollen</subject><subject>Polymorphism</subject><subject>Populations</subject><subject>Single nucleotide polymorphisms</subject><subject>Single-nucleotide polymorphism</subject><subject>Sterility</subject><issn>0014-2336</issn><issn>1573-5060</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kMFKAzEQhoMoWKsP4G3B89ZJskl2j6VoFQoetHgM2exsSWmzNdkW2qc3ZQW9yBzmMP83zHyE3FOYUAD1GCnIkuZAq5xxyfPTBRlRoXguQMIlGQHQIk24vCY3Ma4BoFICRuRzjh57ZzPjzeYYXcy6NkN_cKHzW_R9HtFH17sDZiv0Kbc1G8xij8GlHpzFbO8bDNny_S8Wb8lVazYR7376mCyfnz5mL_nibf46my5yy4Xo84q2Ja9twRoqGyGUBKaqChqmWllWwAtb2LLEsjBYG26Bm6pm1pRUFgU2teVj8jDs3YXua4-x1-tuH9IvUTOqBCulgCqlJkNqla7XzrddH4xN1eDW2c5jm77RU0ULUFwlTWNCB8CGLsaArd4FtzXhqCnos3A9CNdJuD4L16fEsIGJKetXGH5P-R_6Bq4fg-s</recordid><startdate>20190201</startdate><enddate>20190201</enddate><creator>De Guzman, Christian T.</creator><creator>Linscombe, Steven D.</creator><creator>Oard, James H.</creator><general>Springer Netherlands</general><general>Springer</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SN</scope><scope>7SS</scope><scope>7T7</scope><scope>7TM</scope><scope>7X2</scope><scope>7XB</scope><scope>88I</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>M0K</scope><scope>M2P</scope><scope>M7N</scope><scope>P64</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>RC3</scope></search><sort><creationdate>20190201</creationdate><title>Genetic analysis of environment-sensitive genic male sterile rice under US environments</title><author>De Guzman, Christian T. ; Linscombe, Steven D. ; Oard, James H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c355t-91f83bc42d16d5576027990d27f689034c4c88e84aeba3c03a9b2ca81644edbc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Analysis</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Daylength</topic><topic>Genetic analysis</topic><topic>Genetic aspects</topic><topic>Genetic control</topic><topic>High temperature</topic><topic>Hybrid systems</topic><topic>Life Sciences</topic><topic>Nuclease</topic><topic>Oryza sativa</topic><topic>Plant breeding</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Pollen</topic><topic>Polymorphism</topic><topic>Populations</topic><topic>Single nucleotide polymorphisms</topic><topic>Single-nucleotide polymorphism</topic><topic>Sterility</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>De Guzman, Christian T.</creatorcontrib><creatorcontrib>Linscombe, Steven D.</creatorcontrib><creatorcontrib>Oard, James H.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Science Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>Agricultural Science Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><jtitle>Euphytica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>De Guzman, Christian T.</au><au>Linscombe, Steven D.</au><au>Oard, James H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic analysis of environment-sensitive genic male sterile rice under US environments</atitle><jtitle>Euphytica</jtitle><stitle>Euphytica</stitle><date>2019-02-01</date><risdate>2019</risdate><volume>215</volume><issue>2</issue><spage>1</spage><epage>8</epage><pages>1-8</pages><artnum>39</artnum><issn>0014-2336</issn><eissn>1573-5060</eissn><abstract>Two line hybrid rice (
Oryza sativa
L.) breeding uses environment-sensitive genic male sterile (EGMS) lines to produce sterile or fertile pollen depending on daylength and/or temperature. There is limited information on the performance and genetic control of EGMS lines under U.S. environments. Therefore, genetic characterization of two F
2
and four BC
1
F
2
populations derived from EGMS line 2009S was conducted under Louisiana field conditions. Chi squared analyses in the F
2
and BC
1
F
2
populations indicated that pollen sterility under high temperature and long daylength field conditions was controlled by a single recessive gene. Sequence comparisons at locus LOC_Os02g12290 between 2009S, CL161 (USDA-AMS
2002
) and published sequences of G63S and Nipponbare revealed a single nucleotide polymorphism (SNP) that has been detected previously in several EGMS lines. Due to high GC content, a CEL1 nuclease assay was used to detect SNPs associated with pollen sterility in 177 F
2
and 59 BC
1
F
2
sampled individuals. A high percentage of lines (90–100%) across all segregating populations were identified correctly as pollen sterile using the CEL 1 assay. Results from this study suggest that single-gene control of pollen sterility in EGMS line 2009S will be compatible with a two-line system for U.S. hybrid rice development.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s10681-019-2363-z</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-2336 |
| ispartof | Euphytica, 2019-02, Vol.215 (2), p.1-8, Article 39 |
| issn | 0014-2336 1573-5060 |
| language | eng |
| recordid | cdi_proquest_journals_2175286509 |
| source | SpringerNature Journals |
| subjects | Analysis Biomedical and Life Sciences Biotechnology Daylength Genetic analysis Genetic aspects Genetic control High temperature Hybrid systems Life Sciences Nuclease Oryza sativa Plant breeding Plant Genetics and Genomics Plant Pathology Plant Physiology Plant Sciences Pollen Polymorphism Populations Single nucleotide polymorphisms Single-nucleotide polymorphism Sterility |
| title | Genetic analysis of environment-sensitive genic male sterile rice under US environments |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T15%3A16%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genetic%20analysis%20of%20environment-sensitive%20genic%20male%20sterile%20rice%20under%20US%20environments&rft.jtitle=Euphytica&rft.au=De%20Guzman,%20Christian%20T.&rft.date=2019-02-01&rft.volume=215&rft.issue=2&rft.spage=1&rft.epage=8&rft.pages=1-8&rft.artnum=39&rft.issn=0014-2336&rft.eissn=1573-5060&rft_id=info:doi/10.1007/s10681-019-2363-z&rft_dat=%3Cgale_proqu%3EA714073723%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2175286509&rft_id=info:pmid/&rft_galeid=A714073723&rfr_iscdi=true |