Monitoring Cl- movement in single cells exposed to hypotonic solution

Self-referencing ion--selective electrodes (ISEs), made with Chloride Ionophore I-Cocktail A (Fluka), were positioned 1-3 microm from human embryonic kidney cells (tsA201a) and used to record chloride flux during a sustained hyposmotic challenge. The ISE response was close to Nernstian when comparin...

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Veröffentlicht in:	The Journal of membrane biology 2005-01, Vol.203 (2), p.101-110
Hauptverfasser:	Garber, S S, Messerli, M A, Hubert, M, Lewis, R, Hammar, K, Indyk, E, Smith, P J S
Format:	Artikel
Sprache:	eng
Schlagworte:	Cell Line Chlorides - metabolism Epithelial Cells - cytology Epithelial Cells - physiology Humans Hypotonic Solutions - pharmacology Ion Transport - drug effects Ion Transport - physiology
Online-Zugang:	Volltext
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