Identification of heat tolerance loci in broccoli through bulked segregant analysis using whole genome resequencing
Most broccoli cultivars are sensitive to high temperatures during the early stages of floral development causing a severe decline of head quality or even complete lack of head formation under superoptimal crop production temperatures. Several heat tolerant lines have been developed in recent years b...
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| Veröffentlicht in: | Euphytica 2019-02, Vol.215 (2), p.1-9, Article 34 |
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| creator | Branham, Sandra E. Farnham, Mark W. |
| description | Most broccoli cultivars are sensitive to high temperatures during the early stages of floral development causing a severe decline of head quality or even complete lack of head formation under superoptimal crop production temperatures. Several heat tolerant lines have been developed in recent years but there have been few studies of the genetic basis of this complex, polygenic trait. A doubled haploid population of broccoli was evaluated for head quality across two summer field trials with the phenotypic extremes validated in two additional summer fields. Whole-genome resequencing of the bulked segregants was used for a quantitative trait loci (QTL)-seq analysis of heat tolerance. Two novel QTL, which differ from previously reported QTL, were identified. Nonsynonymous SNPs were found in a block of flowering time genes within QHT_C09.2 and may explain the significant negative correlation between time to head maturity and heat tolerance. Breeding further genetic gains in this complex, polygenic trait could be expedited through marker assisted selection and gene pyramiding using markers developed from the QTL identified herein. |
| doi_str_mv | 10.1007/s10681-018-2334-9 |
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Several heat tolerant lines have been developed in recent years but there have been few studies of the genetic basis of this complex, polygenic trait. A doubled haploid population of broccoli was evaluated for head quality across two summer field trials with the phenotypic extremes validated in two additional summer fields. Whole-genome resequencing of the bulked segregants was used for a quantitative trait loci (QTL)-seq analysis of heat tolerance. Two novel QTL, which differ from previously reported QTL, were identified. Nonsynonymous SNPs were found in a block of flowering time genes within QHT_C09.2 and may explain the significant negative correlation between time to head maturity and heat tolerance. Breeding further genetic gains in this complex, polygenic trait could be expedited through marker assisted selection and gene pyramiding using markers developed from the QTL identified herein.</description><identifier>ISSN: 0014-2336</identifier><identifier>EISSN: 1573-5060</identifier><identifier>DOI: 10.1007/s10681-018-2334-9</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Analysis ; Biomedical and Life Sciences ; Biotechnology ; Broccoli ; Crop production ; Cultivars ; Developmental stages ; Flowering ; Gene mapping ; Genetic aspects ; Genetic improvement ; Genetic research ; Genomes ; Genomics ; Heat ; Heat tolerance ; High temperature ; Life Sciences ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Plant Sciences ; Polygenic inheritance ; Quantitative genetics ; Quantitative trait loci ; Single-nucleotide polymorphism ; Vegetables</subject><ispartof>Euphytica, 2019-02, Vol.215 (2), p.1-9, Article 34</ispartof><rights>This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2019</rights><rights>COPYRIGHT 2019 Springer</rights><rights>Euphytica is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c355t-65d0503dc9106a3e557526eec9f68ad7e322a66ad50c18802cf0f21453183b343</citedby><cites>FETCH-LOGICAL-c355t-65d0503dc9106a3e557526eec9f68ad7e322a66ad50c18802cf0f21453183b343</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10681-018-2334-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10681-018-2334-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Branham, Sandra E.</creatorcontrib><creatorcontrib>Farnham, Mark W.</creatorcontrib><title>Identification of heat tolerance loci in broccoli through bulked segregant analysis using whole genome resequencing</title><title>Euphytica</title><addtitle>Euphytica</addtitle><description>Most broccoli cultivars are sensitive to high temperatures during the early stages of floral development causing a severe decline of head quality or even complete lack of head formation under superoptimal crop production temperatures. Several heat tolerant lines have been developed in recent years but there have been few studies of the genetic basis of this complex, polygenic trait. A doubled haploid population of broccoli was evaluated for head quality across two summer field trials with the phenotypic extremes validated in two additional summer fields. Whole-genome resequencing of the bulked segregants was used for a quantitative trait loci (QTL)-seq analysis of heat tolerance. Two novel QTL, which differ from previously reported QTL, were identified. Nonsynonymous SNPs were found in a block of flowering time genes within QHT_C09.2 and may explain the significant negative correlation between time to head maturity and heat tolerance. Breeding further genetic gains in this complex, polygenic trait could be expedited through marker assisted selection and gene pyramiding using markers developed from the QTL identified herein.</description><subject>Analysis</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Broccoli</subject><subject>Crop production</subject><subject>Cultivars</subject><subject>Developmental stages</subject><subject>Flowering</subject><subject>Gene mapping</subject><subject>Genetic aspects</subject><subject>Genetic improvement</subject><subject>Genetic research</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Heat</subject><subject>Heat tolerance</subject><subject>High temperature</subject><subject>Life Sciences</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Polygenic inheritance</subject><subject>Quantitative genetics</subject><subject>Quantitative trait loci</subject><subject>Single-nucleotide polymorphism</subject><subject>Vegetables</subject><issn>0014-2336</issn><issn>1573-5060</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kU1r3DAQhkVJIJuPH5CboGdvRpJl2ccQ-rGw0EtzFlp55FXqlVJJpuy_rxYXegpzGJh5n_ngJeSRwZYBqKfMoOtZA6xvuBBtM3wiGyaVaCR0cEU2AKy9dLobcpvzGwAMSsKG5N2IoXjnrSk-BhodPaIptMQZkwkW6Rytpz7QQ4rWxtnTckxxmY70sMy_cKQZp4STCYWaYOZz9pku2YeJ_jnWGXTCEE9IE2b8vWCwtXNPrp2ZMz78y3fk9euXny_fm_2Pb7uX531jhZSl6eQIEsRoh_qbESilkrxDtIPrejMqFJybrjOjBMv6Hrh14DhrpWC9OIhW3JHP69z3FOvuXPRbXFI9MmvOVMukED2vqu2qmsyM2gcXSzK2xognb2NA52v9WbEWlFCCVYCtgE0x54ROvyd_MumsGeiLGXo1Q1cz9MUMPVSGr0yu2jBh-n_Kx9BfrYeNOA</recordid><startdate>20190201</startdate><enddate>20190201</enddate><creator>Branham, Sandra E.</creator><creator>Farnham, Mark W.</creator><general>Springer Netherlands</general><general>Springer</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SN</scope><scope>7SS</scope><scope>7T7</scope><scope>7TM</scope><scope>7X2</scope><scope>7XB</scope><scope>88I</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>M0K</scope><scope>M2P</scope><scope>M7N</scope><scope>P64</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>RC3</scope></search><sort><creationdate>20190201</creationdate><title>Identification of heat tolerance loci in broccoli through bulked segregant analysis using whole genome resequencing</title><author>Branham, Sandra E. ; Farnham, Mark W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c355t-65d0503dc9106a3e557526eec9f68ad7e322a66ad50c18802cf0f21453183b343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Analysis</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Broccoli</topic><topic>Crop production</topic><topic>Cultivars</topic><topic>Developmental stages</topic><topic>Flowering</topic><topic>Gene mapping</topic><topic>Genetic aspects</topic><topic>Genetic improvement</topic><topic>Genetic research</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Heat</topic><topic>Heat tolerance</topic><topic>High temperature</topic><topic>Life Sciences</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Polygenic inheritance</topic><topic>Quantitative genetics</topic><topic>Quantitative trait loci</topic><topic>Single-nucleotide polymorphism</topic><topic>Vegetables</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Branham, Sandra E.</creatorcontrib><creatorcontrib>Farnham, Mark W.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Science Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>Agricultural Science Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><jtitle>Euphytica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Branham, Sandra E.</au><au>Farnham, Mark W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of heat tolerance loci in broccoli through bulked segregant analysis using whole genome resequencing</atitle><jtitle>Euphytica</jtitle><stitle>Euphytica</stitle><date>2019-02-01</date><risdate>2019</risdate><volume>215</volume><issue>2</issue><spage>1</spage><epage>9</epage><pages>1-9</pages><artnum>34</artnum><issn>0014-2336</issn><eissn>1573-5060</eissn><abstract>Most broccoli cultivars are sensitive to high temperatures during the early stages of floral development causing a severe decline of head quality or even complete lack of head formation under superoptimal crop production temperatures. Several heat tolerant lines have been developed in recent years but there have been few studies of the genetic basis of this complex, polygenic trait. A doubled haploid population of broccoli was evaluated for head quality across two summer field trials with the phenotypic extremes validated in two additional summer fields. Whole-genome resequencing of the bulked segregants was used for a quantitative trait loci (QTL)-seq analysis of heat tolerance. Two novel QTL, which differ from previously reported QTL, were identified. Nonsynonymous SNPs were found in a block of flowering time genes within QHT_C09.2 and may explain the significant negative correlation between time to head maturity and heat tolerance. Breeding further genetic gains in this complex, polygenic trait could be expedited through marker assisted selection and gene pyramiding using markers developed from the QTL identified herein.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s10681-018-2334-9</doi><tpages>9</tpages></addata></record> |
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| subjects | Analysis Biomedical and Life Sciences Biotechnology Broccoli Crop production Cultivars Developmental stages Flowering Gene mapping Genetic aspects Genetic improvement Genetic research Genomes Genomics Heat Heat tolerance High temperature Life Sciences Plant Genetics and Genomics Plant Pathology Plant Physiology Plant Sciences Polygenic inheritance Quantitative genetics Quantitative trait loci Single-nucleotide polymorphism Vegetables |
| title | Identification of heat tolerance loci in broccoli through bulked segregant analysis using whole genome resequencing |
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