Heterologous overexpression of Lithospermum erythrorhizon LeERF-1 gene increases drought and pathogen resistance in Arabidopsis
Ethylene-responsive transcription factors (ERFs) belonging to the AP2/ERF family have diverse functions in plants. However, the functions of LeERF-1 , a member of ERF family from the medicinal plant Lithospermum erythrorhizon , remain unclear. In this study, by cloning the promoter of LeERF-1 , we f...
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| Veröffentlicht in: | Acta physiologiae plantarum 2019-02, Vol.41 (2), Article 24 |
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| creator | Fang, Rong-Jun Li, Ai-Qian Tian, Ru-Nan Zhang, Wen-Ju Zou, Ai-Lan Wu, Feng-Yao Liao, Yong-Hui Wang, Xiao-Ming Pang, Yan-Jun Yang, Rong-Wu Lu, Gui-Hua Qi, Jin-Liang Yang, Yong-Hua |
| description | Ethylene-responsive transcription factors (ERFs) belonging to the AP2/ERF family have diverse functions in plants. However, the functions of
LeERF-1
, a member of ERF family from the medicinal plant
Lithospermum erythrorhizon
, remain unclear. In this study, by cloning the promoter of
LeERF-1
, we found that the promoter region contained a number of potential regulatory motifs related to drought and pathogen resistances. Further transgenic studies showed that the heterologous overexpression of
LeERF-1
in
Arabidopsis
displayed phenotypes of higher survival ratio, lower root inhibition rate, slow water loss in leaf discs, and smaller stomatal apertures under drought stress, compared with wild type (WT) of
Arabidopsis. LeERF-1
transgenic
Arabidopsis
also displayed fewer chlorotic symptoms, lower incidence rates, and lower levels of bacterial proliferation on leaves after the inoculation of bacterial pathogen compared with WT. These results suggested that
LeERF-1
can also confer drought and pathogen resistances. Our work provided a candidate gene with remarkable potential use in genetic engineering for stress resistance improvement in plants. |
| doi_str_mv | 10.1007/s11738-019-2807-z |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2171210183</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2171210183</sourcerecordid><originalsourceid>FETCH-LOGICAL-c359t-c1f9f9ed52db57d2366d1544116360c36dc20d0012fa4556b5df15d06533180a3</originalsourceid><addsrcrecordid>eNp1kE1LAzEQhoMoWD9-gLeA52hm02S3x1JaKxQE0XNIN7PtlnazTnbF9uJfN6WCJ08DM8_zDryM3YF8ACnzxwiQq0JIGImskLk4nLEBFAYEGDM8ZwMJKhe6KOCSXcW4kVIrbcyAfc-xQwrbsAp95OETCb9awhjr0PBQ8UXdrUNskXb9jiPtuzUFWteHdF3g9HUmgK-wQV43JaGLGLmn0K_WHXeN561LdrrzlFjHzjXlkeRjcsvahzbtbthF5bYRb3_nNXufTd8mc7F4eXqejBeiVHrUiRKqUTVCrzO_1LnPlDEe9HAIYJSRpTK-zKSXErLKDbU2S-0r0F4arRQU0qlrdn_KbSl89Bg7uwk9NemlzSCHDCQUKlFwokoKMRJWtqV652hvQdpjz_bUs00922PP9pCc7OTExDYrpL_k_6UfzgyDCA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2171210183</pqid></control><display><type>article</type><title>Heterologous overexpression of Lithospermum erythrorhizon LeERF-1 gene increases drought and pathogen resistance in Arabidopsis</title><source>SpringerLink Journals - AutoHoldings</source><creator>Fang, Rong-Jun ; Li, Ai-Qian ; Tian, Ru-Nan ; Zhang, Wen-Ju ; Zou, Ai-Lan ; Wu, Feng-Yao ; Liao, Yong-Hui ; Wang, Xiao-Ming ; Pang, Yan-Jun ; Yang, Rong-Wu ; Lu, Gui-Hua ; Qi, Jin-Liang ; Yang, Yong-Hua</creator><creatorcontrib>Fang, Rong-Jun ; Li, Ai-Qian ; Tian, Ru-Nan ; Zhang, Wen-Ju ; Zou, Ai-Lan ; Wu, Feng-Yao ; Liao, Yong-Hui ; Wang, Xiao-Ming ; Pang, Yan-Jun ; Yang, Rong-Wu ; Lu, Gui-Hua ; Qi, Jin-Liang ; Yang, Yong-Hua</creatorcontrib><description>Ethylene-responsive transcription factors (ERFs) belonging to the AP2/ERF family have diverse functions in plants. However, the functions of
LeERF-1
, a member of ERF family from the medicinal plant
Lithospermum erythrorhizon
, remain unclear. In this study, by cloning the promoter of
LeERF-1
, we found that the promoter region contained a number of potential regulatory motifs related to drought and pathogen resistances. Further transgenic studies showed that the heterologous overexpression of
LeERF-1
in
Arabidopsis
displayed phenotypes of higher survival ratio, lower root inhibition rate, slow water loss in leaf discs, and smaller stomatal apertures under drought stress, compared with wild type (WT) of
Arabidopsis. LeERF-1
transgenic
Arabidopsis
also displayed fewer chlorotic symptoms, lower incidence rates, and lower levels of bacterial proliferation on leaves after the inoculation of bacterial pathogen compared with WT. These results suggested that
LeERF-1
can also confer drought and pathogen resistances. Our work provided a candidate gene with remarkable potential use in genetic engineering for stress resistance improvement in plants.</description><identifier>ISSN: 0137-5881</identifier><identifier>EISSN: 1861-1664</identifier><identifier>DOI: 10.1007/s11738-019-2807-z</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Agriculture ; Apertures ; Arabidopsis ; Biomedical and Life Sciences ; Cloning ; Drought ; Genetic engineering ; Herbal medicine ; Inoculation ; Leaves ; Life Sciences ; Lithospermum erythrorhizon ; Medicinal plants ; Original Article ; Pathogens ; Phenotypes ; Plant Anatomy/Development ; Plant Biochemistry ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Signs and symptoms ; Stomata ; Transcription factors ; Water loss</subject><ispartof>Acta physiologiae plantarum, 2019-02, Vol.41 (2), Article 24</ispartof><rights>Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2019</rights><rights>Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2019.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c359t-c1f9f9ed52db57d2366d1544116360c36dc20d0012fa4556b5df15d06533180a3</citedby><cites>FETCH-LOGICAL-c359t-c1f9f9ed52db57d2366d1544116360c36dc20d0012fa4556b5df15d06533180a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11738-019-2807-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11738-019-2807-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids></links><search><creatorcontrib>Fang, Rong-Jun</creatorcontrib><creatorcontrib>Li, Ai-Qian</creatorcontrib><creatorcontrib>Tian, Ru-Nan</creatorcontrib><creatorcontrib>Zhang, Wen-Ju</creatorcontrib><creatorcontrib>Zou, Ai-Lan</creatorcontrib><creatorcontrib>Wu, Feng-Yao</creatorcontrib><creatorcontrib>Liao, Yong-Hui</creatorcontrib><creatorcontrib>Wang, Xiao-Ming</creatorcontrib><creatorcontrib>Pang, Yan-Jun</creatorcontrib><creatorcontrib>Yang, Rong-Wu</creatorcontrib><creatorcontrib>Lu, Gui-Hua</creatorcontrib><creatorcontrib>Qi, Jin-Liang</creatorcontrib><creatorcontrib>Yang, Yong-Hua</creatorcontrib><title>Heterologous overexpression of Lithospermum erythrorhizon LeERF-1 gene increases drought and pathogen resistance in Arabidopsis</title><title>Acta physiologiae plantarum</title><addtitle>Acta Physiol Plant</addtitle><description>Ethylene-responsive transcription factors (ERFs) belonging to the AP2/ERF family have diverse functions in plants. However, the functions of
LeERF-1
, a member of ERF family from the medicinal plant
Lithospermum erythrorhizon
, remain unclear. In this study, by cloning the promoter of
LeERF-1
, we found that the promoter region contained a number of potential regulatory motifs related to drought and pathogen resistances. Further transgenic studies showed that the heterologous overexpression of
LeERF-1
in
Arabidopsis
displayed phenotypes of higher survival ratio, lower root inhibition rate, slow water loss in leaf discs, and smaller stomatal apertures under drought stress, compared with wild type (WT) of
Arabidopsis. LeERF-1
transgenic
Arabidopsis
also displayed fewer chlorotic symptoms, lower incidence rates, and lower levels of bacterial proliferation on leaves after the inoculation of bacterial pathogen compared with WT. These results suggested that
LeERF-1
can also confer drought and pathogen resistances. Our work provided a candidate gene with remarkable potential use in genetic engineering for stress resistance improvement in plants.</description><subject>Agriculture</subject><subject>Apertures</subject><subject>Arabidopsis</subject><subject>Biomedical and Life Sciences</subject><subject>Cloning</subject><subject>Drought</subject><subject>Genetic engineering</subject><subject>Herbal medicine</subject><subject>Inoculation</subject><subject>Leaves</subject><subject>Life Sciences</subject><subject>Lithospermum erythrorhizon</subject><subject>Medicinal plants</subject><subject>Original Article</subject><subject>Pathogens</subject><subject>Phenotypes</subject><subject>Plant Anatomy/Development</subject><subject>Plant Biochemistry</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Signs and symptoms</subject><subject>Stomata</subject><subject>Transcription factors</subject><subject>Water loss</subject><issn>0137-5881</issn><issn>1861-1664</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kE1LAzEQhoMoWD9-gLeA52hm02S3x1JaKxQE0XNIN7PtlnazTnbF9uJfN6WCJ08DM8_zDryM3YF8ACnzxwiQq0JIGImskLk4nLEBFAYEGDM8ZwMJKhe6KOCSXcW4kVIrbcyAfc-xQwrbsAp95OETCb9awhjr0PBQ8UXdrUNskXb9jiPtuzUFWteHdF3g9HUmgK-wQV43JaGLGLmn0K_WHXeN561LdrrzlFjHzjXlkeRjcsvahzbtbthF5bYRb3_nNXufTd8mc7F4eXqejBeiVHrUiRKqUTVCrzO_1LnPlDEe9HAIYJSRpTK-zKSXErLKDbU2S-0r0F4arRQU0qlrdn_KbSl89Bg7uwk9NemlzSCHDCQUKlFwokoKMRJWtqV652hvQdpjz_bUs00922PP9pCc7OTExDYrpL_k_6UfzgyDCA</recordid><startdate>20190201</startdate><enddate>20190201</enddate><creator>Fang, Rong-Jun</creator><creator>Li, Ai-Qian</creator><creator>Tian, Ru-Nan</creator><creator>Zhang, Wen-Ju</creator><creator>Zou, Ai-Lan</creator><creator>Wu, Feng-Yao</creator><creator>Liao, Yong-Hui</creator><creator>Wang, Xiao-Ming</creator><creator>Pang, Yan-Jun</creator><creator>Yang, Rong-Wu</creator><creator>Lu, Gui-Hua</creator><creator>Qi, Jin-Liang</creator><creator>Yang, Yong-Hua</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20190201</creationdate><title>Heterologous overexpression of Lithospermum erythrorhizon LeERF-1 gene increases drought and pathogen resistance in Arabidopsis</title><author>Fang, Rong-Jun ; Li, Ai-Qian ; Tian, Ru-Nan ; Zhang, Wen-Ju ; Zou, Ai-Lan ; Wu, Feng-Yao ; Liao, Yong-Hui ; Wang, Xiao-Ming ; Pang, Yan-Jun ; Yang, Rong-Wu ; Lu, Gui-Hua ; Qi, Jin-Liang ; Yang, Yong-Hua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-c1f9f9ed52db57d2366d1544116360c36dc20d0012fa4556b5df15d06533180a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Agriculture</topic><topic>Apertures</topic><topic>Arabidopsis</topic><topic>Biomedical and Life Sciences</topic><topic>Cloning</topic><topic>Drought</topic><topic>Genetic engineering</topic><topic>Herbal medicine</topic><topic>Inoculation</topic><topic>Leaves</topic><topic>Life Sciences</topic><topic>Lithospermum erythrorhizon</topic><topic>Medicinal plants</topic><topic>Original Article</topic><topic>Pathogens</topic><topic>Phenotypes</topic><topic>Plant Anatomy/Development</topic><topic>Plant Biochemistry</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Signs and symptoms</topic><topic>Stomata</topic><topic>Transcription factors</topic><topic>Water loss</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fang, Rong-Jun</creatorcontrib><creatorcontrib>Li, Ai-Qian</creatorcontrib><creatorcontrib>Tian, Ru-Nan</creatorcontrib><creatorcontrib>Zhang, Wen-Ju</creatorcontrib><creatorcontrib>Zou, Ai-Lan</creatorcontrib><creatorcontrib>Wu, Feng-Yao</creatorcontrib><creatorcontrib>Liao, Yong-Hui</creatorcontrib><creatorcontrib>Wang, Xiao-Ming</creatorcontrib><creatorcontrib>Pang, Yan-Jun</creatorcontrib><creatorcontrib>Yang, Rong-Wu</creatorcontrib><creatorcontrib>Lu, Gui-Hua</creatorcontrib><creatorcontrib>Qi, Jin-Liang</creatorcontrib><creatorcontrib>Yang, Yong-Hua</creatorcontrib><collection>CrossRef</collection><jtitle>Acta physiologiae plantarum</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fang, Rong-Jun</au><au>Li, Ai-Qian</au><au>Tian, Ru-Nan</au><au>Zhang, Wen-Ju</au><au>Zou, Ai-Lan</au><au>Wu, Feng-Yao</au><au>Liao, Yong-Hui</au><au>Wang, Xiao-Ming</au><au>Pang, Yan-Jun</au><au>Yang, Rong-Wu</au><au>Lu, Gui-Hua</au><au>Qi, Jin-Liang</au><au>Yang, Yong-Hua</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Heterologous overexpression of Lithospermum erythrorhizon LeERF-1 gene increases drought and pathogen resistance in Arabidopsis</atitle><jtitle>Acta physiologiae plantarum</jtitle><stitle>Acta Physiol Plant</stitle><date>2019-02-01</date><risdate>2019</risdate><volume>41</volume><issue>2</issue><artnum>24</artnum><issn>0137-5881</issn><eissn>1861-1664</eissn><abstract>Ethylene-responsive transcription factors (ERFs) belonging to the AP2/ERF family have diverse functions in plants. However, the functions of
LeERF-1
, a member of ERF family from the medicinal plant
Lithospermum erythrorhizon
, remain unclear. In this study, by cloning the promoter of
LeERF-1
, we found that the promoter region contained a number of potential regulatory motifs related to drought and pathogen resistances. Further transgenic studies showed that the heterologous overexpression of
LeERF-1
in
Arabidopsis
displayed phenotypes of higher survival ratio, lower root inhibition rate, slow water loss in leaf discs, and smaller stomatal apertures under drought stress, compared with wild type (WT) of
Arabidopsis. LeERF-1
transgenic
Arabidopsis
also displayed fewer chlorotic symptoms, lower incidence rates, and lower levels of bacterial proliferation on leaves after the inoculation of bacterial pathogen compared with WT. These results suggested that
LeERF-1
can also confer drought and pathogen resistances. Our work provided a candidate gene with remarkable potential use in genetic engineering for stress resistance improvement in plants.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><doi>10.1007/s11738-019-2807-z</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Agriculture Apertures Arabidopsis Biomedical and Life Sciences Cloning Drought Genetic engineering Herbal medicine Inoculation Leaves Life Sciences Lithospermum erythrorhizon Medicinal plants Original Article Pathogens Phenotypes Plant Anatomy/Development Plant Biochemistry Plant Genetics and Genomics Plant Pathology Plant Physiology Signs and symptoms Stomata Transcription factors Water loss |
| title | Heterologous overexpression of Lithospermum erythrorhizon LeERF-1 gene increases drought and pathogen resistance in Arabidopsis |
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