Detection of Strawberry crinkle virus in plants and aphids by RT‐PCR using conserved L gene sequences

About 10% of the large (L) protein gene of Strawberry crinkle virus (SCV) was sequenced after amplification with degenerate primers designed to conserved regions of the rhabdovirus L protein. The virus sequence was extended to 1362 nucleotides through rapid amplification of cDNA ends. One pair of de...

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Veröffentlicht in:	Plant pathology 2002-06, Vol.51 (3), p.266-274
Hauptverfasser:	Posthuma, K. I., Adams, A. N., Hong, Y., Kirby, M. J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Fragaria Fundamental and applied biological sciences. Psychology Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control Microbiology Mononegavirales Phytopathology. Animal pests. Plant and forest protection Plant viruses and viroids Rhabdoviridae RNA‐dependent RNA polymerase strawberry Strawberry crinkle virus Techniques used in virology Virology
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