Detection of Strawberry crinkle virus in plants and aphids by RT‐PCR using conserved L gene sequences

Detection of Strawberry crinkle virus in plants and aphids by RT‐PCR using conserved L gene sequences

About 10% of the large (L) protein gene of Strawberry crinkle virus (SCV) was sequenced after amplification with degenerate primers designed to conserved regions of the rhabdovirus L protein. The virus sequence was extended to 1362 nucleotides through rapid amplification of cDNA ends. One pair of de...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Plant pathology 2002-06, Vol.51 (3), p.266-274
Hauptverfasser: Posthuma, K. I., Adams, A. N., Hong, Y., Kirby, M. J.
Format: Artikel
Sprache:eng
Schlagworte:
Biological and medical sciences
Fragaria
Fundamental and applied biological sciences. Psychology
Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control
Microbiology
Mononegavirales
Phytopathology. Animal pests. Plant and forest protection
Plant viruses and viroids
Rhabdoviridae
RNA‐dependent RNA polymerase
strawberry
Strawberry crinkle virus
Techniques used in virology
Virology
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:About 10% of the large (L) protein gene of Strawberry crinkle virus (SCV) was sequenced after amplification with degenerate primers designed to conserved regions of the rhabdovirus L protein. The virus sequence was extended to 1362 nucleotides through rapid amplification of cDNA ends. One pair of degenerate L gene primers amplified a 683‐bp fragment from four different isolates of SCV cultured in the experimental host Physalis pubescens; the nucleotide sequences of these fragments differed by
ISSN:0032-0862
1365-3059
DOI:10.1046/j.1365-3059.2002.00725.x