Novel in vivo use of a polyvalent Streptomyces phage to disinfest Streptomyces scabies‐infected seed potatoes
A highly virulent and polyvalent Streptomyces phage was isolated from a potato field near Albany, Western Australia. The efficacy of the isolated phage to disinfest seed potato tubers artificially inoculated with a common scab‐causing streptomycete was evaluated. The phage suspension was prepared in...
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| Veröffentlicht in: | Plant pathology 2001-12, Vol.50 (6), p.666-675 |
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| creator | McKenna, F. El‐Tarabily, K. A. Hardy, G. E. ST. J. Dell, B. |
| description | A highly virulent and polyvalent Streptomyces phage was isolated from a potato field near Albany, Western Australia. The efficacy of the isolated phage to disinfest seed potato tubers artificially inoculated with a common scab‐causing streptomycete was evaluated. The phage suspension was prepared in a mini‐bioreactor. Diseased potatoes were bathed in a phage suspension (1 × 109 plaque‐forming units per mL) for 24 h. The suspension was constantly circulated within a novel 25 L phage bath by means of an air‐sparging pipe driven from an air compressor. Phage‐treated scab‐affected seed potatoes planted into free‐draining polystyrene boxes containing steam‐pasteurized field soil produced tuber progeny with significantly (P |
| doi_str_mv | 10.1046/j.1365-3059.2001.00648.x |
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Phage‐treated scab‐affected seed potatoes planted into free‐draining polystyrene boxes containing steam‐pasteurized field soil produced tuber progeny with significantly (P < 0·05) reduced levels of surface lesions of scab (1·2%) compared with tubers harvested from nonphage‐treated tubers (23%). The number of scab lesions was also significantly reduced (P < 0·05) by phage treatment of mother tubers. No significant differences were recorded in weight, size or number of harvested tubers from phage‐treated or nontreated mother tubers. This is the first in vivo study that has used Streptomyces phage to significantly disinfest seed potatoes of Streptomyces scabies and thereby reduce contamination of soil from seed‐tuber‐borne inoculum and reduce infection of daughter tubers.</description><identifier>ISSN: 0032-0862</identifier><identifier>EISSN: 1365-3059</identifier><identifier>DOI: 10.1046/j.1365-3059.2001.00648.x</identifier><identifier>CODEN: PLPAAD</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Bacterial plant pathogens ; Biological and medical sciences ; biological control ; bioreactor ; Biotechnology ; common scab ; Fundamental and applied biological sciences. Psychology ; Generalities. Techniques. Transmission, epidemiology, ecology. Antibacterial substances, control ; phage bath ; Phytopathology. Animal pests. 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A.</creatorcontrib><creatorcontrib>Hardy, G. E. ST. J.</creatorcontrib><creatorcontrib>Dell, B.</creatorcontrib><title>Novel in vivo use of a polyvalent Streptomyces phage to disinfest Streptomyces scabies‐infected seed potatoes</title><title>Plant pathology</title><description>A highly virulent and polyvalent Streptomyces phage was isolated from a potato field near Albany, Western Australia. The efficacy of the isolated phage to disinfest seed potato tubers artificially inoculated with a common scab‐causing streptomycete was evaluated. The phage suspension was prepared in a mini‐bioreactor. Diseased potatoes were bathed in a phage suspension (1 × 109 plaque‐forming units per mL) for 24 h. The suspension was constantly circulated within a novel 25 L phage bath by means of an air‐sparging pipe driven from an air compressor. Phage‐treated scab‐affected seed potatoes planted into free‐draining polystyrene boxes containing steam‐pasteurized field soil produced tuber progeny with significantly (P < 0·05) reduced levels of surface lesions of scab (1·2%) compared with tubers harvested from nonphage‐treated tubers (23%). The number of scab lesions was also significantly reduced (P < 0·05) by phage treatment of mother tubers. No significant differences were recorded in weight, size or number of harvested tubers from phage‐treated or nontreated mother tubers. This is the first in vivo study that has used Streptomyces phage to significantly disinfest seed potatoes of Streptomyces scabies and thereby reduce contamination of soil from seed‐tuber‐borne inoculum and reduce infection of daughter tubers.</description><subject>Bacterial plant pathogens</subject><subject>Biological and medical sciences</subject><subject>biological control</subject><subject>bioreactor</subject><subject>Biotechnology</subject><subject>common scab</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Generalities. Techniques. Transmission, epidemiology, ecology. Antibacterial substances, control</subject><subject>phage bath</subject><subject>Phytopathology. Animal pests. 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J. ; Dell, B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4838-8a5c31ff984ad80be12c93dd5bed475df94717e3da4427e544de0dff3e41a7773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Bacterial plant pathogens</topic><topic>Biological and medical sciences</topic><topic>biological control</topic><topic>bioreactor</topic><topic>Biotechnology</topic><topic>common scab</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Generalities. Techniques. Transmission, epidemiology, ecology. Antibacterial substances, control</topic><topic>phage bath</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>potato tuber</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>McKenna, F.</creatorcontrib><creatorcontrib>El‐Tarabily, K. A.</creatorcontrib><creatorcontrib>Hardy, G. E. ST. J.</creatorcontrib><creatorcontrib>Dell, B.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Plant pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>McKenna, F.</au><au>El‐Tarabily, K. A.</au><au>Hardy, G. E. ST. J.</au><au>Dell, B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Novel in vivo use of a polyvalent Streptomyces phage to disinfest Streptomyces scabies‐infected seed potatoes</atitle><jtitle>Plant pathology</jtitle><date>2001-12</date><risdate>2001</risdate><volume>50</volume><issue>6</issue><spage>666</spage><epage>675</epage><pages>666-675</pages><issn>0032-0862</issn><eissn>1365-3059</eissn><coden>PLPAAD</coden><abstract>A highly virulent and polyvalent Streptomyces phage was isolated from a potato field near Albany, Western Australia. The efficacy of the isolated phage to disinfest seed potato tubers artificially inoculated with a common scab‐causing streptomycete was evaluated. The phage suspension was prepared in a mini‐bioreactor. Diseased potatoes were bathed in a phage suspension (1 × 109 plaque‐forming units per mL) for 24 h. The suspension was constantly circulated within a novel 25 L phage bath by means of an air‐sparging pipe driven from an air compressor. Phage‐treated scab‐affected seed potatoes planted into free‐draining polystyrene boxes containing steam‐pasteurized field soil produced tuber progeny with significantly (P < 0·05) reduced levels of surface lesions of scab (1·2%) compared with tubers harvested from nonphage‐treated tubers (23%). The number of scab lesions was also significantly reduced (P < 0·05) by phage treatment of mother tubers. No significant differences were recorded in weight, size or number of harvested tubers from phage‐treated or nontreated mother tubers. This is the first in vivo study that has used Streptomyces phage to significantly disinfest seed potatoes of Streptomyces scabies and thereby reduce contamination of soil from seed‐tuber‐borne inoculum and reduce infection of daughter tubers.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><doi>10.1046/j.1365-3059.2001.00648.x</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Bacterial plant pathogens Biological and medical sciences biological control bioreactor Biotechnology common scab Fundamental and applied biological sciences. Psychology Generalities. Techniques. Transmission, epidemiology, ecology. Antibacterial substances, control phage bath Phytopathology. Animal pests. Plant and forest protection potato tuber |
| title | Novel in vivo use of a polyvalent Streptomyces phage to disinfest Streptomyces scabies‐infected seed potatoes |
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