Effects of cigarette smoke condensate on proliferation and pluripotency gene expression in mouse embryonic stem cells
Background Embryonic stem cells (ESCs) are derived from the inner cell mass (ICM) of blastocysts. They can be used as valuable experimental models to test the effects of drugs, chemicals, and environmental contaminants such as cigarette smoke condensate (CSC) on preimplantation embryo development. T...
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| Veröffentlicht in: | Journal of cellular biochemistry 2019-03, Vol.120 (3), p.4071-4080 |
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| creator | Assadollahi, Vahideh Mohammadi, Ebrahim Fathi, Fardin hassanzadeh, Kambiz Erfan, Mohamad Bager Khadem Soleimani, Farzad Banafshi, Omid Yousefi, Fayegh Allahvaisi, Ozra |
| description | Background
Embryonic stem cells (ESCs) are derived from the inner cell mass (ICM) of blastocysts. They can be used as valuable experimental models to test the effects of drugs, chemicals, and environmental contaminants such as cigarette smoke condensate (CSC) on preimplantation embryo development. The aim of this study was to evaluate the effect of CSC on ESCs derived from mice with different genetic backgrounds and maternal ages.
Methods
The study groups consisted of mouse ESCs (mESCs) obtained from three sources: blastocysts developed from fertilized oocytes of two‐month‐old (2‐C57) and six‐month‐old (6‐C57) C57BL/6 inbred mice and those developed from fertilized oocytes of two‐month‐old (2‐NMRI) NMRI outbred mice. The groups of mESCs were exposed to 0.04, 4, and 40 μg/mL CSC. After exposure, we measured cell viability by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide assay and real‐time polymerase chain reaction for changes in expressions of
Oct4, Sox2, Nanog, Ahr, Bax, Bcl2, TFAM, and
POLG. The cell doubling time (DT) of these populations was also determined.
Results
We observed that CSC changed proliferation and DT in the 2‐C57 and 6‐C57 cells. There was no change in 2‐NMRI cells. Exposure to CSC caused changes in the gene expressions and induced apoptosis in all three cell lines.
Conclusion
Based on the results of the study, it can be concluded that CSC has an effect on the viability, DT and gene expression patterns in mouse ESCs and its effects vary based on the genetic background and maternal age of isolated mouse ESCs.
Embryonic stem cells (ESCs) are derived from the inner cell mass (ICM) of blastocysts. They can be used as valuable experimental models to test the effects of drugs, chemicals, and environmental contaminants such as cigarette smoke condensate (CSC) on preimplantation embryo development. The aim of this study was to evaluate the effect of CSC on ESCs derived from mice with different genetic backgrounds and maternal ages. |
| doi_str_mv | 10.1002/jcb.27692 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2167054620</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2167054620</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3532-de659bde1f1f8b554dc10daaa72762cbdd2bd530f6b65d1300e02a2b48300003</originalsourceid><addsrcrecordid>eNp1kElPxDAMhSMEgmE58AdQJE4cyjhJF3qEEatG4sK9yuKgDm1SklYw_54MA9w42X769Gw_Qk4ZXDIAPl9pdcmrsuY7ZMagrrK8zPNdMoNKQMYF4wfkMMYVANS14PvkQAAva1GxGZlurUU9Ruot1e2rDDiOSGPv35Bq7wy6KJPgHR2C71qLQY5tmqQzdOim0A5-RKfX9BUdUvwcAsa4AVpHez_FpPUqrL1rNY0j9lRj18VjsmdlF_Hkpx6Rl7vbl8VDtny-f1xcLzMtCsEzg2VRK4PMMnuliiI3moGRUlbpW66VMVyZQoAtVVkYJgAQuOQqv0otgDgi51vbdPv7hHFsVn4KLm1sOCsrKPKSb6iLLaWDjzGgbYbQ9jKsGwbNJt8m5dt855vYsx_HSfVo_sjfQBMw3wIfbYfr_52ap8XN1vILTkyGZg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2167054620</pqid></control><display><type>article</type><title>Effects of cigarette smoke condensate on proliferation and pluripotency gene expression in mouse embryonic stem cells</title><source>Wiley Blackwell Single Titles</source><creator>Assadollahi, Vahideh ; Mohammadi, Ebrahim ; Fathi, Fardin ; hassanzadeh, Kambiz ; Erfan, Mohamad Bager Khadem ; Soleimani, Farzad ; Banafshi, Omid ; Yousefi, Fayegh ; Allahvaisi, Ozra</creator><creatorcontrib>Assadollahi, Vahideh ; Mohammadi, Ebrahim ; Fathi, Fardin ; hassanzadeh, Kambiz ; Erfan, Mohamad Bager Khadem ; Soleimani, Farzad ; Banafshi, Omid ; Yousefi, Fayegh ; Allahvaisi, Ozra</creatorcontrib><description>Background
Embryonic stem cells (ESCs) are derived from the inner cell mass (ICM) of blastocysts. They can be used as valuable experimental models to test the effects of drugs, chemicals, and environmental contaminants such as cigarette smoke condensate (CSC) on preimplantation embryo development. The aim of this study was to evaluate the effect of CSC on ESCs derived from mice with different genetic backgrounds and maternal ages.
Methods
The study groups consisted of mouse ESCs (mESCs) obtained from three sources: blastocysts developed from fertilized oocytes of two‐month‐old (2‐C57) and six‐month‐old (6‐C57) C57BL/6 inbred mice and those developed from fertilized oocytes of two‐month‐old (2‐NMRI) NMRI outbred mice. The groups of mESCs were exposed to 0.04, 4, and 40 μg/mL CSC. After exposure, we measured cell viability by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide assay and real‐time polymerase chain reaction for changes in expressions of
Oct4, Sox2, Nanog, Ahr, Bax, Bcl2, TFAM, and
POLG. The cell doubling time (DT) of these populations was also determined.
Results
We observed that CSC changed proliferation and DT in the 2‐C57 and 6‐C57 cells. There was no change in 2‐NMRI cells. Exposure to CSC caused changes in the gene expressions and induced apoptosis in all three cell lines.
Conclusion
Based on the results of the study, it can be concluded that CSC has an effect on the viability, DT and gene expression patterns in mouse ESCs and its effects vary based on the genetic background and maternal age of isolated mouse ESCs.
Embryonic stem cells (ESCs) are derived from the inner cell mass (ICM) of blastocysts. They can be used as valuable experimental models to test the effects of drugs, chemicals, and environmental contaminants such as cigarette smoke condensate (CSC) on preimplantation embryo development. The aim of this study was to evaluate the effect of CSC on ESCs derived from mice with different genetic backgrounds and maternal ages.</description><identifier>ISSN: 0730-2312</identifier><identifier>EISSN: 1097-4644</identifier><identifier>DOI: 10.1002/jcb.27692</identifier><identifier>PMID: 30269371</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Animal models ; Apoptosis ; aryl hydrocarbon receptor ; Blastocysts ; C57BL/6 mice ; Cell lines ; Cell proliferation ; cigarette ; Cigarette smoke ; Condensates ; Contaminants ; Embryo cells ; embryonic stem cell ; Embryos ; Exposure ; Gene expression ; Inbreeding ; maternal age ; Mice ; Oct-4 protein ; Oocytes ; Organic chemistry ; outbred strain ; Pluripotency ; Polymerase chain reaction ; Smoke ; smoking ; Stem cell transplantation ; Stem cells</subject><ispartof>Journal of cellular biochemistry, 2019-03, Vol.120 (3), p.4071-4080</ispartof><rights>2018 Wiley Periodicals, Inc.</rights><rights>2019 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3532-de659bde1f1f8b554dc10daaa72762cbdd2bd530f6b65d1300e02a2b48300003</citedby><cites>FETCH-LOGICAL-c3532-de659bde1f1f8b554dc10daaa72762cbdd2bd530f6b65d1300e02a2b48300003</cites><orcidid>0000-0002-4648-5598</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjcb.27692$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjcb.27692$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30269371$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Assadollahi, Vahideh</creatorcontrib><creatorcontrib>Mohammadi, Ebrahim</creatorcontrib><creatorcontrib>Fathi, Fardin</creatorcontrib><creatorcontrib>hassanzadeh, Kambiz</creatorcontrib><creatorcontrib>Erfan, Mohamad Bager Khadem</creatorcontrib><creatorcontrib>Soleimani, Farzad</creatorcontrib><creatorcontrib>Banafshi, Omid</creatorcontrib><creatorcontrib>Yousefi, Fayegh</creatorcontrib><creatorcontrib>Allahvaisi, Ozra</creatorcontrib><title>Effects of cigarette smoke condensate on proliferation and pluripotency gene expression in mouse embryonic stem cells</title><title>Journal of cellular biochemistry</title><addtitle>J Cell Biochem</addtitle><description>Background
Embryonic stem cells (ESCs) are derived from the inner cell mass (ICM) of blastocysts. They can be used as valuable experimental models to test the effects of drugs, chemicals, and environmental contaminants such as cigarette smoke condensate (CSC) on preimplantation embryo development. The aim of this study was to evaluate the effect of CSC on ESCs derived from mice with different genetic backgrounds and maternal ages.
Methods
The study groups consisted of mouse ESCs (mESCs) obtained from three sources: blastocysts developed from fertilized oocytes of two‐month‐old (2‐C57) and six‐month‐old (6‐C57) C57BL/6 inbred mice and those developed from fertilized oocytes of two‐month‐old (2‐NMRI) NMRI outbred mice. The groups of mESCs were exposed to 0.04, 4, and 40 μg/mL CSC. After exposure, we measured cell viability by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide assay and real‐time polymerase chain reaction for changes in expressions of
Oct4, Sox2, Nanog, Ahr, Bax, Bcl2, TFAM, and
POLG. The cell doubling time (DT) of these populations was also determined.
Results
We observed that CSC changed proliferation and DT in the 2‐C57 and 6‐C57 cells. There was no change in 2‐NMRI cells. Exposure to CSC caused changes in the gene expressions and induced apoptosis in all three cell lines.
Conclusion
Based on the results of the study, it can be concluded that CSC has an effect on the viability, DT and gene expression patterns in mouse ESCs and its effects vary based on the genetic background and maternal age of isolated mouse ESCs.
Embryonic stem cells (ESCs) are derived from the inner cell mass (ICM) of blastocysts. They can be used as valuable experimental models to test the effects of drugs, chemicals, and environmental contaminants such as cigarette smoke condensate (CSC) on preimplantation embryo development. The aim of this study was to evaluate the effect of CSC on ESCs derived from mice with different genetic backgrounds and maternal ages.</description><subject>Animal models</subject><subject>Apoptosis</subject><subject>aryl hydrocarbon receptor</subject><subject>Blastocysts</subject><subject>C57BL/6 mice</subject><subject>Cell lines</subject><subject>Cell proliferation</subject><subject>cigarette</subject><subject>Cigarette smoke</subject><subject>Condensates</subject><subject>Contaminants</subject><subject>Embryo cells</subject><subject>embryonic stem cell</subject><subject>Embryos</subject><subject>Exposure</subject><subject>Gene expression</subject><subject>Inbreeding</subject><subject>maternal age</subject><subject>Mice</subject><subject>Oct-4 protein</subject><subject>Oocytes</subject><subject>Organic chemistry</subject><subject>outbred strain</subject><subject>Pluripotency</subject><subject>Polymerase chain reaction</subject><subject>Smoke</subject><subject>smoking</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><issn>0730-2312</issn><issn>1097-4644</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kElPxDAMhSMEgmE58AdQJE4cyjhJF3qEEatG4sK9yuKgDm1SklYw_54MA9w42X769Gw_Qk4ZXDIAPl9pdcmrsuY7ZMagrrK8zPNdMoNKQMYF4wfkMMYVANS14PvkQAAva1GxGZlurUU9Ruot1e2rDDiOSGPv35Bq7wy6KJPgHR2C71qLQY5tmqQzdOim0A5-RKfX9BUdUvwcAsa4AVpHez_FpPUqrL1rNY0j9lRj18VjsmdlF_Hkpx6Rl7vbl8VDtny-f1xcLzMtCsEzg2VRK4PMMnuliiI3moGRUlbpW66VMVyZQoAtVVkYJgAQuOQqv0otgDgi51vbdPv7hHFsVn4KLm1sOCsrKPKSb6iLLaWDjzGgbYbQ9jKsGwbNJt8m5dt855vYsx_HSfVo_sjfQBMw3wIfbYfr_52ap8XN1vILTkyGZg</recordid><startdate>201903</startdate><enddate>201903</enddate><creator>Assadollahi, Vahideh</creator><creator>Mohammadi, Ebrahim</creator><creator>Fathi, Fardin</creator><creator>hassanzadeh, Kambiz</creator><creator>Erfan, Mohamad Bager Khadem</creator><creator>Soleimani, Farzad</creator><creator>Banafshi, Omid</creator><creator>Yousefi, Fayegh</creator><creator>Allahvaisi, Ozra</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><orcidid>https://orcid.org/0000-0002-4648-5598</orcidid></search><sort><creationdate>201903</creationdate><title>Effects of cigarette smoke condensate on proliferation and pluripotency gene expression in mouse embryonic stem cells</title><author>Assadollahi, Vahideh ; Mohammadi, Ebrahim ; Fathi, Fardin ; hassanzadeh, Kambiz ; Erfan, Mohamad Bager Khadem ; Soleimani, Farzad ; Banafshi, Omid ; Yousefi, Fayegh ; Allahvaisi, Ozra</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3532-de659bde1f1f8b554dc10daaa72762cbdd2bd530f6b65d1300e02a2b48300003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animal models</topic><topic>Apoptosis</topic><topic>aryl hydrocarbon receptor</topic><topic>Blastocysts</topic><topic>C57BL/6 mice</topic><topic>Cell lines</topic><topic>Cell proliferation</topic><topic>cigarette</topic><topic>Cigarette smoke</topic><topic>Condensates</topic><topic>Contaminants</topic><topic>Embryo cells</topic><topic>embryonic stem cell</topic><topic>Embryos</topic><topic>Exposure</topic><topic>Gene expression</topic><topic>Inbreeding</topic><topic>maternal age</topic><topic>Mice</topic><topic>Oct-4 protein</topic><topic>Oocytes</topic><topic>Organic chemistry</topic><topic>outbred strain</topic><topic>Pluripotency</topic><topic>Polymerase chain reaction</topic><topic>Smoke</topic><topic>smoking</topic><topic>Stem cell transplantation</topic><topic>Stem cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Assadollahi, Vahideh</creatorcontrib><creatorcontrib>Mohammadi, Ebrahim</creatorcontrib><creatorcontrib>Fathi, Fardin</creatorcontrib><creatorcontrib>hassanzadeh, Kambiz</creatorcontrib><creatorcontrib>Erfan, Mohamad Bager Khadem</creatorcontrib><creatorcontrib>Soleimani, Farzad</creatorcontrib><creatorcontrib>Banafshi, Omid</creatorcontrib><creatorcontrib>Yousefi, Fayegh</creatorcontrib><creatorcontrib>Allahvaisi, Ozra</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of cellular biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Assadollahi, Vahideh</au><au>Mohammadi, Ebrahim</au><au>Fathi, Fardin</au><au>hassanzadeh, Kambiz</au><au>Erfan, Mohamad Bager Khadem</au><au>Soleimani, Farzad</au><au>Banafshi, Omid</au><au>Yousefi, Fayegh</au><au>Allahvaisi, Ozra</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of cigarette smoke condensate on proliferation and pluripotency gene expression in mouse embryonic stem cells</atitle><jtitle>Journal of cellular biochemistry</jtitle><addtitle>J Cell Biochem</addtitle><date>2019-03</date><risdate>2019</risdate><volume>120</volume><issue>3</issue><spage>4071</spage><epage>4080</epage><pages>4071-4080</pages><issn>0730-2312</issn><eissn>1097-4644</eissn><abstract>Background
Embryonic stem cells (ESCs) are derived from the inner cell mass (ICM) of blastocysts. They can be used as valuable experimental models to test the effects of drugs, chemicals, and environmental contaminants such as cigarette smoke condensate (CSC) on preimplantation embryo development. The aim of this study was to evaluate the effect of CSC on ESCs derived from mice with different genetic backgrounds and maternal ages.
Methods
The study groups consisted of mouse ESCs (mESCs) obtained from three sources: blastocysts developed from fertilized oocytes of two‐month‐old (2‐C57) and six‐month‐old (6‐C57) C57BL/6 inbred mice and those developed from fertilized oocytes of two‐month‐old (2‐NMRI) NMRI outbred mice. The groups of mESCs were exposed to 0.04, 4, and 40 μg/mL CSC. After exposure, we measured cell viability by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide assay and real‐time polymerase chain reaction for changes in expressions of
Oct4, Sox2, Nanog, Ahr, Bax, Bcl2, TFAM, and
POLG. The cell doubling time (DT) of these populations was also determined.
Results
We observed that CSC changed proliferation and DT in the 2‐C57 and 6‐C57 cells. There was no change in 2‐NMRI cells. Exposure to CSC caused changes in the gene expressions and induced apoptosis in all three cell lines.
Conclusion
Based on the results of the study, it can be concluded that CSC has an effect on the viability, DT and gene expression patterns in mouse ESCs and its effects vary based on the genetic background and maternal age of isolated mouse ESCs.
Embryonic stem cells (ESCs) are derived from the inner cell mass (ICM) of blastocysts. They can be used as valuable experimental models to test the effects of drugs, chemicals, and environmental contaminants such as cigarette smoke condensate (CSC) on preimplantation embryo development. The aim of this study was to evaluate the effect of CSC on ESCs derived from mice with different genetic backgrounds and maternal ages.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>30269371</pmid><doi>10.1002/jcb.27692</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-4648-5598</orcidid></addata></record> |
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| subjects | Animal models Apoptosis aryl hydrocarbon receptor Blastocysts C57BL/6 mice Cell lines Cell proliferation cigarette Cigarette smoke Condensates Contaminants Embryo cells embryonic stem cell Embryos Exposure Gene expression Inbreeding maternal age Mice Oct-4 protein Oocytes Organic chemistry outbred strain Pluripotency Polymerase chain reaction Smoke smoking Stem cell transplantation Stem cells |
| title | Effects of cigarette smoke condensate on proliferation and pluripotency gene expression in mouse embryonic stem cells |
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