TRANSPOSON MUTAGENESIS AND CLONING ANALYSIS OF MamZ GENE IN MAGNETOSPIRILLUM GRYPHISWALDENSE MSR-1

A magnetosome-deletion mutant strain of Magnetospirillum gryphiswaldense MSR-1, known as NM7, was obtained by means of a mini-Tn5 lac Z2 transposon insertion in conjugation experiments. A 4642-bp fragment containing a mini-Tn5 lac Z2 in NM7 was cloned through the sequential steps of digestion, self-...

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Veröffentlicht in:	Fresenius environmental bulletin 2018-05, Vol.27 (5), p.2724
Hauptverfasser:	Zhou, Lei, Guo, Ruixue, Wu, Sha, Xu, Junjie, Xiong, Minghua, Li, Ying, Li, Feng, Wang, Guangli
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino acid sequence Clonal deletion Cloning Conjugation Deletion mutant Gene deletion Genetic transformation Insertion Iron Proteins Transposon mutagenesis Transposons
Online-Zugang:	Volltext
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