CRISPR/Cas9 knock-in of GST-tagged human Noggin in the β-casein gene locus of bovine ear fibroblast cells

We developed knock-in vector system of human Noggin mature sequence with glutathione S-transferase (GST) containing factor Xa protease linker to facilitate the subsequent purification of recombinant protein. To achieve this, bovine ear fibroblast cells were isolated and transfection conditions were...

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