The Release of Membrane Vesicles with Mature MicroRNA-221 and Activated Caspase-3 by Platelets during Storage of Platelet Concentrate
— The preparation and storage of platelet concentrate can be accompanied by activation of platelets and triggering of apoptosis, increasing the formation of platelet membrane vesicles and worsening the therapeutic properties of such preparations. However, the mechanisms for the regulation of apoptos...
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| Veröffentlicht in: | Cell and tissue biology 2018-11, Vol.12 (6), p.506-509 |
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| creator | Kishenko, V. V. Kondratov, K. A. Mikhailovsky, V. Yu Sidorkevich, S. V. Vavilova, T. V. Sirotkina, O. V. Fedorov, A. V. |
| description | —
The preparation and storage of platelet concentrate can be accompanied by activation of platelets and triggering of apoptosis, increasing the formation of platelet membrane vesicles and worsening the therapeutic properties of such preparations. However, the mechanisms for the regulation of apoptosis in platelets and the molecular composition of platelet membrane vesicles associated with this process have so far remained poorly understood. The aim of the work was to study proapoptotic caspase-3 and antiapoptotic microRNA-221 levels in platelets and platelet membrane vesicles during the storage of platelet concentrate preparations. The object of the study was samples of the pooled platelet concentrates (
n
= 6), taken from the containers on the second and seventh days of storage. Full length and activated caspase-3 were found in platelets and in fractions of membrane vesicles. The proportion of activated caspase-3 in platelets increased during storage. MicroRNA-221 was found in platelets, fractions of membrane vesicles, as well as in the extravesicular fraction. During storage, levels of microRNA-221 increased 8-fold (
P
= 0.004) in the fraction of membrane vesicles pelleted at 100 000
g
. The results of the study indicate that the membrane vesicles released by platelets during storage contain mature microRNA-221 and the activated form of caspase-3. |
| doi_str_mv | 10.1134/S1990519X18060056 |
| format | Article |
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The preparation and storage of platelet concentrate can be accompanied by activation of platelets and triggering of apoptosis, increasing the formation of platelet membrane vesicles and worsening the therapeutic properties of such preparations. However, the mechanisms for the regulation of apoptosis in platelets and the molecular composition of platelet membrane vesicles associated with this process have so far remained poorly understood. The aim of the work was to study proapoptotic caspase-3 and antiapoptotic microRNA-221 levels in platelets and platelet membrane vesicles during the storage of platelet concentrate preparations. The object of the study was samples of the pooled platelet concentrates (
n
= 6), taken from the containers on the second and seventh days of storage. Full length and activated caspase-3 were found in platelets and in fractions of membrane vesicles. The proportion of activated caspase-3 in platelets increased during storage. MicroRNA-221 was found in platelets, fractions of membrane vesicles, as well as in the extravesicular fraction. During storage, levels of microRNA-221 increased 8-fold (
P
= 0.004) in the fraction of membrane vesicles pelleted at 100 000
g
. The results of the study indicate that the membrane vesicles released by platelets during storage contain mature microRNA-221 and the activated form of caspase-3.</description><identifier>ISSN: 1990-519X</identifier><identifier>EISSN: 1990-5203</identifier><identifier>DOI: 10.1134/S1990519X18060056</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>Apoptosis ; Bile ; Biomedical and Life Sciences ; Caspase ; Caspase-3 ; Cell Biology ; Life Sciences ; Membrane vesicles ; MicroRNAs ; miRNA ; Platelets</subject><ispartof>Cell and tissue biology, 2018-11, Vol.12 (6), p.506-509</ispartof><rights>Pleiades Publishing, Ltd. 2018</rights><rights>Copyright Springer Science & Business Media 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c1836-268a74b4d73bdcf467bfa24358ab0e5e2516c6b4b4d8074560bb90ec8b9e52523</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S1990519X18060056$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S1990519X18060056$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Kishenko, V. V.</creatorcontrib><creatorcontrib>Kondratov, K. A.</creatorcontrib><creatorcontrib>Mikhailovsky, V. Yu</creatorcontrib><creatorcontrib>Sidorkevich, S. V.</creatorcontrib><creatorcontrib>Vavilova, T. V.</creatorcontrib><creatorcontrib>Sirotkina, O. V.</creatorcontrib><creatorcontrib>Fedorov, A. V.</creatorcontrib><title>The Release of Membrane Vesicles with Mature MicroRNA-221 and Activated Caspase-3 by Platelets during Storage of Platelet Concentrate</title><title>Cell and tissue biology</title><addtitle>Cell Tiss. Biol</addtitle><description>—
The preparation and storage of platelet concentrate can be accompanied by activation of platelets and triggering of apoptosis, increasing the formation of platelet membrane vesicles and worsening the therapeutic properties of such preparations. However, the mechanisms for the regulation of apoptosis in platelets and the molecular composition of platelet membrane vesicles associated with this process have so far remained poorly understood. The aim of the work was to study proapoptotic caspase-3 and antiapoptotic microRNA-221 levels in platelets and platelet membrane vesicles during the storage of platelet concentrate preparations. The object of the study was samples of the pooled platelet concentrates (
n
= 6), taken from the containers on the second and seventh days of storage. Full length and activated caspase-3 were found in platelets and in fractions of membrane vesicles. The proportion of activated caspase-3 in platelets increased during storage. MicroRNA-221 was found in platelets, fractions of membrane vesicles, as well as in the extravesicular fraction. During storage, levels of microRNA-221 increased 8-fold (
P
= 0.004) in the fraction of membrane vesicles pelleted at 100 000
g
. The results of the study indicate that the membrane vesicles released by platelets during storage contain mature microRNA-221 and the activated form of caspase-3.</description><subject>Apoptosis</subject><subject>Bile</subject><subject>Biomedical and Life Sciences</subject><subject>Caspase</subject><subject>Caspase-3</subject><subject>Cell Biology</subject><subject>Life Sciences</subject><subject>Membrane vesicles</subject><subject>MicroRNAs</subject><subject>miRNA</subject><subject>Platelets</subject><issn>1990-519X</issn><issn>1990-5203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1UFtLwzAUDqLgnP4A3wI-V5O0SdvHUbzBprJN8a0k6enW0bUzSZX9AP-3qXP4ID6dy3fjHITOKbmkNIyuZjRNCafpK02IIISLAzToVwFnJDzc9x4_RifWrognRZQM0Od8CXgKNUgLuC3xBNbKyAbwC9hK12DxR-WWeCJdZwBPKm3a6cMoYIxi2RR4pF31Lh0UOJN24z2CEKstfqr9rgZncdGZqlngmWuNXHwn7DGctY2Gxhk_nqKjUtYWzn7qED3fXM-zu2D8eHufjcaBpkkoAiYSGUcqKuJQFbqMRKxKyaKQJ1IR4MA4FVqonpGQOOKCKJUS0IlKgTPOwiG62PluTPvWgXX5qu1M4yNzRgUlMU_j2LPojuWPtdZAmW9MtZZmm1OS99_O_3zba9hOYzf9wWB-nf8XfQEGYIEA</recordid><startdate>20181101</startdate><enddate>20181101</enddate><creator>Kishenko, V. V.</creator><creator>Kondratov, K. A.</creator><creator>Mikhailovsky, V. Yu</creator><creator>Sidorkevich, S. V.</creator><creator>Vavilova, T. V.</creator><creator>Sirotkina, O. V.</creator><creator>Fedorov, A. V.</creator><general>Pleiades Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20181101</creationdate><title>The Release of Membrane Vesicles with Mature MicroRNA-221 and Activated Caspase-3 by Platelets during Storage of Platelet Concentrate</title><author>Kishenko, V. V. ; Kondratov, K. A. ; Mikhailovsky, V. Yu ; Sidorkevich, S. V. ; Vavilova, T. V. ; Sirotkina, O. V. ; Fedorov, A. V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1836-268a74b4d73bdcf467bfa24358ab0e5e2516c6b4b4d8074560bb90ec8b9e52523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Apoptosis</topic><topic>Bile</topic><topic>Biomedical and Life Sciences</topic><topic>Caspase</topic><topic>Caspase-3</topic><topic>Cell Biology</topic><topic>Life Sciences</topic><topic>Membrane vesicles</topic><topic>MicroRNAs</topic><topic>miRNA</topic><topic>Platelets</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kishenko, V. V.</creatorcontrib><creatorcontrib>Kondratov, K. A.</creatorcontrib><creatorcontrib>Mikhailovsky, V. Yu</creatorcontrib><creatorcontrib>Sidorkevich, S. V.</creatorcontrib><creatorcontrib>Vavilova, T. V.</creatorcontrib><creatorcontrib>Sirotkina, O. V.</creatorcontrib><creatorcontrib>Fedorov, A. V.</creatorcontrib><collection>CrossRef</collection><jtitle>Cell and tissue biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kishenko, V. V.</au><au>Kondratov, K. A.</au><au>Mikhailovsky, V. Yu</au><au>Sidorkevich, S. V.</au><au>Vavilova, T. V.</au><au>Sirotkina, O. V.</au><au>Fedorov, A. V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Release of Membrane Vesicles with Mature MicroRNA-221 and Activated Caspase-3 by Platelets during Storage of Platelet Concentrate</atitle><jtitle>Cell and tissue biology</jtitle><stitle>Cell Tiss. Biol</stitle><date>2018-11-01</date><risdate>2018</risdate><volume>12</volume><issue>6</issue><spage>506</spage><epage>509</epage><pages>506-509</pages><issn>1990-519X</issn><eissn>1990-5203</eissn><abstract>—
The preparation and storage of platelet concentrate can be accompanied by activation of platelets and triggering of apoptosis, increasing the formation of platelet membrane vesicles and worsening the therapeutic properties of such preparations. However, the mechanisms for the regulation of apoptosis in platelets and the molecular composition of platelet membrane vesicles associated with this process have so far remained poorly understood. The aim of the work was to study proapoptotic caspase-3 and antiapoptotic microRNA-221 levels in platelets and platelet membrane vesicles during the storage of platelet concentrate preparations. The object of the study was samples of the pooled platelet concentrates (
n
= 6), taken from the containers on the second and seventh days of storage. Full length and activated caspase-3 were found in platelets and in fractions of membrane vesicles. The proportion of activated caspase-3 in platelets increased during storage. MicroRNA-221 was found in platelets, fractions of membrane vesicles, as well as in the extravesicular fraction. During storage, levels of microRNA-221 increased 8-fold (
P
= 0.004) in the fraction of membrane vesicles pelleted at 100 000
g
. The results of the study indicate that the membrane vesicles released by platelets during storage contain mature microRNA-221 and the activated form of caspase-3.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><doi>10.1134/S1990519X18060056</doi><tpages>4</tpages></addata></record> |
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| subjects | Apoptosis Bile Biomedical and Life Sciences Caspase Caspase-3 Cell Biology Life Sciences Membrane vesicles MicroRNAs miRNA Platelets |
| title | The Release of Membrane Vesicles with Mature MicroRNA-221 and Activated Caspase-3 by Platelets during Storage of Platelet Concentrate |
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