Design, synthesis and X‐ray structural studies of novel [acetonitrile‐benzyl‐3‐N‐(2, 4 dihydroxyphenylmethylene) hydrazinecarbodithioato‐κ3‐N′, S, O] nickel(ll) complex that potently inhibit cell proliferation through regulation of apoptosis related genes
Nickel is a fundamental element for healthy life for human and higher animals. For biological importance, its complexation with bioactive ligand is worth to be studied with the aim to understand its function. Using mouse peritoneal cancer model, MTT colorimetric assay and anticancer activity analysi...
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creator | Zahan, Ronok Rahi, Md. Sifat Sheikh, Md. Chanmiya Miyatake, Ryuta Zangrando, Ennio Naz, Tarannum Islam, Md. Al‐Amin‐Al‐Azadul Reza, Md Abu |
description | Nickel is a fundamental element for healthy life for human and higher animals. For biological importance, its complexation with bioactive ligand is worth to be studied with the aim to understand its function. Using mouse peritoneal cancer model, MTT colorimetric assay and anticancer activity analysis, we examined the role of nickel(ll) complex in growth inhibition of cancer cells. A novel nickel(ll) complex was synthesized and characterized using physico‐chemical and spectroscopic techniques. The study indicated that both the ligand and complex were capable of inhibiting Ehrlich Ascites Carcinoma (EAC) cells growth by 28.21% and 44.52%, respectively, when administered 0.3 mg/kg/day body weight intraperitoneally for five consecutive days in Swiss Webstar mice. Determination the LD50 of the complex (55 mg/kg) allowed adjusting the dose as 2.75 mg/kg and upon administration, inhibition increased to 69.36%. The ligand and complex have shown an inhibitory effect in the range of 4.86%–67.3% and 6.1%‐ 89.37%, respectively, against EAC cells (concentration range of 31.25–500 μg/ml) in RPMI‐1640 medium as determined by MTT colorimetric assay. Apoptotic cell morphological alteration was determined through optical and fluorescence microscopy. Up regulation of P53, Bax, Cas‐8, Cas‐3 and Fas and down regulation of NF‐kB and Bcl‐2 gene expression were observed in the cells treated with the nickel(ll) complex for five consecutive days. In conclusion, the newly synthesized nickel(ll) complex has shown anti‐proliferative activity and can further be optimized to be used as a lead molecule for anticancer drug.
The synthesized nickel(ll) complex exhibited anticancer activity in in vivo mice model. EAC Cells growthinhibition was confirmed by cellular morphological alteration fluorescence microscope which was supportedby DNA fragmentation that is a hallmark features. The novel synthesized metal complex induces apoptosisthrough a definite pathway. |
doi_str_mv | 10.1002/aoc.4601 |
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The synthesized nickel(ll) complex exhibited anticancer activity in in vivo mice model. EAC Cells growthinhibition was confirmed by cellular morphological alteration fluorescence microscope which was supportedby DNA fragmentation that is a hallmark features. The novel synthesized metal complex induces apoptosisthrough a definite pathway.</description><identifier>ISSN: 0268-2605</identifier><identifier>EISSN: 1099-0739</identifier><identifier>DOI: 10.1002/aoc.4601</identifier><language>eng</language><publisher>Chichester: Wiley Subscription Services, Inc</publisher><subject>Acetonitrile ; anticancer activity ; Anticancer properties ; Apoptosis ; Body weight ; Cancer ; Chemical synthesis ; Chemistry ; Colorimetry ; Coordination compounds ; DNA fragmentation ; Fluorescence ; Gene expression ; Ligands ; Nickel ; nickel(ll) complex ; ONS ligand ; Organic chemistry</subject><ispartof>Applied organometallic chemistry, 2019-01, Vol.33 (1), p.n/a</ispartof><rights>2018 John Wiley & Sons, Ltd.</rights><rights>2019 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3301-4e24b3af0ca0b3609c0625bd313295368e7170b2506b67b020a95b625b39480b3</citedby><cites>FETCH-LOGICAL-c3301-4e24b3af0ca0b3609c0625bd313295368e7170b2506b67b020a95b625b39480b3</cites><orcidid>0000-0002-3146-2772</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Faoc.4601$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Faoc.4601$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27929,27930,45579,45580</link.rule.ids></links><search><creatorcontrib>Zahan, Ronok</creatorcontrib><creatorcontrib>Rahi, Md. Sifat</creatorcontrib><creatorcontrib>Sheikh, Md. Chanmiya</creatorcontrib><creatorcontrib>Miyatake, Ryuta</creatorcontrib><creatorcontrib>Zangrando, Ennio</creatorcontrib><creatorcontrib>Naz, Tarannum</creatorcontrib><creatorcontrib>Islam, Md. Al‐Amin‐Al‐Azadul</creatorcontrib><creatorcontrib>Reza, Md Abu</creatorcontrib><title>Design, synthesis and X‐ray structural studies of novel [acetonitrile‐benzyl‐3‐N‐(2, 4 dihydroxyphenylmethylene) hydrazinecarbodithioato‐κ3‐N′, S, O] nickel(ll) complex that potently inhibit cell proliferation through regulation of apoptosis related genes</title><title>Applied organometallic chemistry</title><description>Nickel is a fundamental element for healthy life for human and higher animals. For biological importance, its complexation with bioactive ligand is worth to be studied with the aim to understand its function. Using mouse peritoneal cancer model, MTT colorimetric assay and anticancer activity analysis, we examined the role of nickel(ll) complex in growth inhibition of cancer cells. A novel nickel(ll) complex was synthesized and characterized using physico‐chemical and spectroscopic techniques. The study indicated that both the ligand and complex were capable of inhibiting Ehrlich Ascites Carcinoma (EAC) cells growth by 28.21% and 44.52%, respectively, when administered 0.3 mg/kg/day body weight intraperitoneally for five consecutive days in Swiss Webstar mice. Determination the LD50 of the complex (55 mg/kg) allowed adjusting the dose as 2.75 mg/kg and upon administration, inhibition increased to 69.36%. The ligand and complex have shown an inhibitory effect in the range of 4.86%–67.3% and 6.1%‐ 89.37%, respectively, against EAC cells (concentration range of 31.25–500 μg/ml) in RPMI‐1640 medium as determined by MTT colorimetric assay. Apoptotic cell morphological alteration was determined through optical and fluorescence microscopy. Up regulation of P53, Bax, Cas‐8, Cas‐3 and Fas and down regulation of NF‐kB and Bcl‐2 gene expression were observed in the cells treated with the nickel(ll) complex for five consecutive days. In conclusion, the newly synthesized nickel(ll) complex has shown anti‐proliferative activity and can further be optimized to be used as a lead molecule for anticancer drug.
The synthesized nickel(ll) complex exhibited anticancer activity in in vivo mice model. EAC Cells growthinhibition was confirmed by cellular morphological alteration fluorescence microscope which was supportedby DNA fragmentation that is a hallmark features. The novel synthesized metal complex induces apoptosisthrough a definite pathway.</description><subject>Acetonitrile</subject><subject>anticancer activity</subject><subject>Anticancer properties</subject><subject>Apoptosis</subject><subject>Body weight</subject><subject>Cancer</subject><subject>Chemical synthesis</subject><subject>Chemistry</subject><subject>Colorimetry</subject><subject>Coordination compounds</subject><subject>DNA fragmentation</subject><subject>Fluorescence</subject><subject>Gene expression</subject><subject>Ligands</subject><subject>Nickel</subject><subject>nickel(ll) complex</subject><subject>ONS ligand</subject><subject>Organic chemistry</subject><issn>0268-2605</issn><issn>1099-0739</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kU2O1DAQhQMCiWZAmiOUxKZH6gyVOEl3lqPmVxrRC0BCQihynErHg9sOtgPjWXEEzsKSQ3AIToJDz5ZFyU9PX5Wr9JLkNMPzDDF_yo04LyrM7iaLDOs6xTWr7yULzKtNmldYPkgeOneFiHWVFYs7p8_Iyb1egQvaD1E74LqDD3--_7A8gPN2En6yXEU5dZIcmB60-UoKPnJB3mjprVQU-Zb0TVBRsFhvYi3zFRTQySF01lyHcSAd1IH8EBRpOoPZ5zdSk-C2NZ30gzTcm9j5-9ftjJ8reLuC3SfQUnwmtVTqDIQ5jIquwQ_cw2g8aa8CSD3IVnoQpBSM1ijZk-VeGh1Ba6b9AJb2kzpa8Qg-mtGb-WBL0aUO9nEr9yi533Pl6PHte5K8f_H83fZVerl7-Xp7cZkKxjBLC8qLlvEeBceWVVgLrPKy7VjG8rpk1YbW2RrbvMSqrdYt5sjrsp0RVheb2HKSPDnOjbt-mcj55spMVscvmzyrkDFW1-tILY-UsMY5S30zWnngNjQZNnPeTcy7mfOOaHpEv8U4wn-55mK3_cf_BXhyuYM</recordid><startdate>201901</startdate><enddate>201901</enddate><creator>Zahan, Ronok</creator><creator>Rahi, Md. Sifat</creator><creator>Sheikh, Md. Chanmiya</creator><creator>Miyatake, Ryuta</creator><creator>Zangrando, Ennio</creator><creator>Naz, Tarannum</creator><creator>Islam, Md. Al‐Amin‐Al‐Azadul</creator><creator>Reza, Md Abu</creator><general>Wiley Subscription Services, Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><orcidid>https://orcid.org/0000-0002-3146-2772</orcidid></search><sort><creationdate>201901</creationdate><title>Design, synthesis and X‐ray structural studies of novel [acetonitrile‐benzyl‐3‐N‐(2, 4 dihydroxyphenylmethylene) hydrazinecarbodithioato‐κ3‐N′, S, O] nickel(ll) complex that potently inhibit cell proliferation through regulation of apoptosis related genes</title><author>Zahan, Ronok ; Rahi, Md. Sifat ; Sheikh, Md. Chanmiya ; Miyatake, Ryuta ; Zangrando, Ennio ; Naz, Tarannum ; Islam, Md. Al‐Amin‐Al‐Azadul ; Reza, Md Abu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3301-4e24b3af0ca0b3609c0625bd313295368e7170b2506b67b020a95b625b39480b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Acetonitrile</topic><topic>anticancer activity</topic><topic>Anticancer properties</topic><topic>Apoptosis</topic><topic>Body weight</topic><topic>Cancer</topic><topic>Chemical synthesis</topic><topic>Chemistry</topic><topic>Colorimetry</topic><topic>Coordination compounds</topic><topic>DNA fragmentation</topic><topic>Fluorescence</topic><topic>Gene expression</topic><topic>Ligands</topic><topic>Nickel</topic><topic>nickel(ll) complex</topic><topic>ONS ligand</topic><topic>Organic chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zahan, Ronok</creatorcontrib><creatorcontrib>Rahi, Md. Sifat</creatorcontrib><creatorcontrib>Sheikh, Md. Chanmiya</creatorcontrib><creatorcontrib>Miyatake, Ryuta</creatorcontrib><creatorcontrib>Zangrando, Ennio</creatorcontrib><creatorcontrib>Naz, Tarannum</creatorcontrib><creatorcontrib>Islam, Md. Al‐Amin‐Al‐Azadul</creatorcontrib><creatorcontrib>Reza, Md Abu</creatorcontrib><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Applied organometallic chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zahan, Ronok</au><au>Rahi, Md. Sifat</au><au>Sheikh, Md. Chanmiya</au><au>Miyatake, Ryuta</au><au>Zangrando, Ennio</au><au>Naz, Tarannum</au><au>Islam, Md. Al‐Amin‐Al‐Azadul</au><au>Reza, Md Abu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Design, synthesis and X‐ray structural studies of novel [acetonitrile‐benzyl‐3‐N‐(2, 4 dihydroxyphenylmethylene) hydrazinecarbodithioato‐κ3‐N′, S, O] nickel(ll) complex that potently inhibit cell proliferation through regulation of apoptosis related genes</atitle><jtitle>Applied organometallic chemistry</jtitle><date>2019-01</date><risdate>2019</risdate><volume>33</volume><issue>1</issue><epage>n/a</epage><issn>0268-2605</issn><eissn>1099-0739</eissn><abstract>Nickel is a fundamental element for healthy life for human and higher animals. For biological importance, its complexation with bioactive ligand is worth to be studied with the aim to understand its function. Using mouse peritoneal cancer model, MTT colorimetric assay and anticancer activity analysis, we examined the role of nickel(ll) complex in growth inhibition of cancer cells. A novel nickel(ll) complex was synthesized and characterized using physico‐chemical and spectroscopic techniques. The study indicated that both the ligand and complex were capable of inhibiting Ehrlich Ascites Carcinoma (EAC) cells growth by 28.21% and 44.52%, respectively, when administered 0.3 mg/kg/day body weight intraperitoneally for five consecutive days in Swiss Webstar mice. Determination the LD50 of the complex (55 mg/kg) allowed adjusting the dose as 2.75 mg/kg and upon administration, inhibition increased to 69.36%. The ligand and complex have shown an inhibitory effect in the range of 4.86%–67.3% and 6.1%‐ 89.37%, respectively, against EAC cells (concentration range of 31.25–500 μg/ml) in RPMI‐1640 medium as determined by MTT colorimetric assay. Apoptotic cell morphological alteration was determined through optical and fluorescence microscopy. Up regulation of P53, Bax, Cas‐8, Cas‐3 and Fas and down regulation of NF‐kB and Bcl‐2 gene expression were observed in the cells treated with the nickel(ll) complex for five consecutive days. In conclusion, the newly synthesized nickel(ll) complex has shown anti‐proliferative activity and can further be optimized to be used as a lead molecule for anticancer drug.
The synthesized nickel(ll) complex exhibited anticancer activity in in vivo mice model. EAC Cells growthinhibition was confirmed by cellular morphological alteration fluorescence microscope which was supportedby DNA fragmentation that is a hallmark features. The novel synthesized metal complex induces apoptosisthrough a definite pathway.</abstract><cop>Chichester</cop><pub>Wiley Subscription Services, Inc</pub><doi>10.1002/aoc.4601</doi><tpages>16</tpages><orcidid>https://orcid.org/0000-0002-3146-2772</orcidid></addata></record> |
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subjects | Acetonitrile anticancer activity Anticancer properties Apoptosis Body weight Cancer Chemical synthesis Chemistry Colorimetry Coordination compounds DNA fragmentation Fluorescence Gene expression Ligands Nickel nickel(ll) complex ONS ligand Organic chemistry |
title | Design, synthesis and X‐ray structural studies of novel [acetonitrile‐benzyl‐3‐N‐(2, 4 dihydroxyphenylmethylene) hydrazinecarbodithioato‐κ3‐N′, S, O] nickel(ll) complex that potently inhibit cell proliferation through regulation of apoptosis related genes |
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