Preparation of Modified Combinatorial DNA Libraries via Emulsion PCR with Subsequent Strand Separation

— A modification of the enzymatic method for the preparation of combinatorial random DNA libraries, which combines amplification in isolated microvolumes with the simultaneous incorporation of modified nucleotides and subsequent separation of DNA strands, was developed. Deoxyuridine triphosphate wit...

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Veröffentlicht in:	Molecular biology (New York) 2018-11, Vol.52 (6), p.854-864
Hauptverfasser:	Lapa, S. A., Romashova, K. S., Spitsyn, M. A., Shershov, V. E., Kuznetsova, V. E., Guseinov, T. O., Zasedateleva, O. A., Radko, S. P., Timofeev, E. N., Lisitsa, A. V., Chudinov, A. V.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino acids Aptamers Biochemistry Biomedical and Life Sciences Combinatorial libraries Deoxyribonucleic acid DNA Genomics. Transcriptomics Human Genetics Hydrophobicity Life Sciences Nucleotides Polymerase chain reaction Single-stranded DNA
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