RAPID, EASY, AND ECONOMICAL SCREENING TEST FOR ANTIBODIES TO HUMAN IMMUNODEFICIENCY VIRUS

A new dot enzyme immunoassay (EIA) with a conserved portion of the envelope protein of the human immunodeficiency virus (HIV) as antigen has been designed for use in areas with few laboratory facilities and by personnel with little laboratory experience. Sera were tested in 263 subjects who had AIDS...

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Veröffentlicht in:The Lancet (British edition) 1987-02, Vol.329 (8529), p.361-362
Hauptverfasser: Carlson, JamesR, Yee, JoannL, Watson-Williams, E.John, Jennings, MyraB, Mertens, SteveC, Gardner, MurrayB, Ghrayeb, John, Biggar, RobertJ
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container_end_page 362
container_issue 8529
container_start_page 361
container_title The Lancet (British edition)
container_volume 329
creator Carlson, JamesR
Yee, JoannL
Watson-Williams, E.John
Jennings, MyraB
Mertens, SteveC
Gardner, MurrayB
Ghrayeb, John
Biggar, RobertJ
description A new dot enzyme immunoassay (EIA) with a conserved portion of the envelope protein of the human immunodeficiency virus (HIV) as antigen has been designed for use in areas with few laboratory facilities and by personnel with little laboratory experience. Sera were tested in 263 subjects who had AIDS or AIDS-related complex or were at-risk or not-at-risk of AIDS from the USA, Africa, and Asia/Oceania. The dot EIA was 100% sensitive in the American subjects, and there were only 2 false negatives in the others, both of which were negative by commercial EIA. The test is simple to perform, economical, rapid (30 min), and stable.
doi_str_mv 10.1016/S0140-6736(87)91730-2
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identifier ISSN: 0140-6736
ispartof The Lancet (British edition), 1987-02, Vol.329 (8529), p.361-362
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source Access via ScienceDirect (Elsevier)
subjects Acquired immune deficiency syndrome
AIDS
AIDS-related complex
Antibodies
Enzyme immunoassay
HIV
Human immunodeficiency virus
Immunoassay
Laboratories
Medical screening
Proteins
Viral envelope proteins
Viruses
title RAPID, EASY, AND ECONOMICAL SCREENING TEST FOR ANTIBODIES TO HUMAN IMMUNODEFICIENCY VIRUS
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