Ty1 Copy Number Dynamics in Saccharomyces
To understand long terminal repeat (LTR)-retrotransposon copy number dynamics, Ty1 elements were reintroduced into a "Ty-less" Saccharomyces strain where elements had been lost by LTR-LTR recombination. Repopulated strains exhibited alterations in chromosome size that were associated with...
Gespeichert in:
| Veröffentlicht in: | Genetics (Austin) 2005-03, Vol.169 (3), p.1845 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 3 |
| container_start_page | 1845 |
| container_title | Genetics (Austin) |
| container_volume | 169 |
| creator | Garfinkel, David J Nyswaner, Katherine M Stefanisko, Karen M Chang, Caroline Moore, Sharon P |
| description | To understand long terminal repeat (LTR)-retrotransposon copy number dynamics, Ty1 elements were reintroduced into a "Ty-less" Saccharomyces strain where elements had been lost by LTR-LTR recombination. Repopulated strains exhibited alterations in chromosome size that were associated with Ty1 insertions, but did not become genetically isolated. The rates of element gain and loss under genetic and environmental conditions known to affect Ty1 retrotransposition were determined using genetically tagged reference elements. The results show that Ty1 retrotransposition varies with copy number, temperature, and cell type. In contrast to retrotransposition, Ty1 loss by LTR-LTR recombination was more constant and not markedly influenced by copy number. Endogenous Ty1 cDNA was poorly utilized for recombination when compared with LTR-LTR recombination or ectopic gene conversion. Ty1 elements also appear to be more susceptible to copy number fluctuation in haploid cells. Ty1 gain/loss ratios obtained under different conditions suggest that copy number oscillates over time by altering the rate of retrotransposition, resulting in the diverse copy numbers observed in Saccharomyces. [PUBLICATION ABSTRACT] |
| format | Article |
| fullrecord | <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_journals_214111916</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1036818271</sourcerecordid><originalsourceid>FETCH-proquest_journals_2141119163</originalsourceid><addsrcrecordid>eNpjYuA0tDQx1jUyMzZkYeA0MDA00zUzNzbkYOAqLs4yMDAwszS14GTQDKk0VHDOL6hU8CvNTUotUnCpzEvMzUwuVsjMUwhOTE7OSCzKz61MTi3mYWBNS8wpTuWF0twMSm6uIc4eugVF-YWlqcUl8Vn5pUV5QKl4I0MTQ0NDS0MzY6IUAQAIVjC3</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>214111916</pqid></control><display><type>article</type><title>Ty1 Copy Number Dynamics in Saccharomyces</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Alma/SFX Local Collection</source><creator>Garfinkel, David J ; Nyswaner, Katherine M ; Stefanisko, Karen M ; Chang, Caroline ; Moore, Sharon P</creator><creatorcontrib>Garfinkel, David J ; Nyswaner, Katherine M ; Stefanisko, Karen M ; Chang, Caroline ; Moore, Sharon P</creatorcontrib><description>To understand long terminal repeat (LTR)-retrotransposon copy number dynamics, Ty1 elements were reintroduced into a "Ty-less" Saccharomyces strain where elements had been lost by LTR-LTR recombination. Repopulated strains exhibited alterations in chromosome size that were associated with Ty1 insertions, but did not become genetically isolated. The rates of element gain and loss under genetic and environmental conditions known to affect Ty1 retrotransposition were determined using genetically tagged reference elements. The results show that Ty1 retrotransposition varies with copy number, temperature, and cell type. In contrast to retrotransposition, Ty1 loss by LTR-LTR recombination was more constant and not markedly influenced by copy number. Endogenous Ty1 cDNA was poorly utilized for recombination when compared with LTR-LTR recombination or ectopic gene conversion. Ty1 elements also appear to be more susceptible to copy number fluctuation in haploid cells. Ty1 gain/loss ratios obtained under different conditions suggest that copy number oscillates over time by altering the rate of retrotransposition, resulting in the diverse copy numbers observed in Saccharomyces. [PUBLICATION ABSTRACT]</description><identifier>ISSN: 0016-6731</identifier><identifier>EISSN: 1943-2631</identifier><identifier>CODEN: GENTAE</identifier><language>eng</language><publisher>Bethesda: Genetics Society of America</publisher><subject>Genetic recombination ; Genetics ; Genomics ; Microbiology ; Yeast</subject><ispartof>Genetics (Austin), 2005-03, Vol.169 (3), p.1845</ispartof><rights>Copyright Genetics Society of America Mar 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>Garfinkel, David J</creatorcontrib><creatorcontrib>Nyswaner, Katherine M</creatorcontrib><creatorcontrib>Stefanisko, Karen M</creatorcontrib><creatorcontrib>Chang, Caroline</creatorcontrib><creatorcontrib>Moore, Sharon P</creatorcontrib><title>Ty1 Copy Number Dynamics in Saccharomyces</title><title>Genetics (Austin)</title><description>To understand long terminal repeat (LTR)-retrotransposon copy number dynamics, Ty1 elements were reintroduced into a "Ty-less" Saccharomyces strain where elements had been lost by LTR-LTR recombination. Repopulated strains exhibited alterations in chromosome size that were associated with Ty1 insertions, but did not become genetically isolated. The rates of element gain and loss under genetic and environmental conditions known to affect Ty1 retrotransposition were determined using genetically tagged reference elements. The results show that Ty1 retrotransposition varies with copy number, temperature, and cell type. In contrast to retrotransposition, Ty1 loss by LTR-LTR recombination was more constant and not markedly influenced by copy number. Endogenous Ty1 cDNA was poorly utilized for recombination when compared with LTR-LTR recombination or ectopic gene conversion. Ty1 elements also appear to be more susceptible to copy number fluctuation in haploid cells. Ty1 gain/loss ratios obtained under different conditions suggest that copy number oscillates over time by altering the rate of retrotransposition, resulting in the diverse copy numbers observed in Saccharomyces. [PUBLICATION ABSTRACT]</description><subject>Genetic recombination</subject><subject>Genetics</subject><subject>Genomics</subject><subject>Microbiology</subject><subject>Yeast</subject><issn>0016-6731</issn><issn>1943-2631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpjYuA0tDQx1jUyMzZkYeA0MDA00zUzNzbkYOAqLs4yMDAwszS14GTQDKk0VHDOL6hU8CvNTUotUnCpzEvMzUwuVsjMUwhOTE7OSCzKz61MTi3mYWBNS8wpTuWF0twMSm6uIc4eugVF-YWlqcUl8Vn5pUV5QKl4I0MTQ0NDS0MzY6IUAQAIVjC3</recordid><startdate>20050301</startdate><enddate>20050301</enddate><creator>Garfinkel, David J</creator><creator>Nyswaner, Katherine M</creator><creator>Stefanisko, Karen M</creator><creator>Chang, Caroline</creator><creator>Moore, Sharon P</creator><general>Genetics Society of America</general><scope>3V.</scope><scope>4T-</scope><scope>4U-</scope><scope>7QP</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope></search><sort><creationdate>20050301</creationdate><title>Ty1 Copy Number Dynamics in Saccharomyces</title><author>Garfinkel, David J ; Nyswaner, Katherine M ; Stefanisko, Karen M ; Chang, Caroline ; Moore, Sharon P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_journals_2141119163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Genetic recombination</topic><topic>Genetics</topic><topic>Genomics</topic><topic>Microbiology</topic><topic>Yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Garfinkel, David J</creatorcontrib><creatorcontrib>Nyswaner, Katherine M</creatorcontrib><creatorcontrib>Stefanisko, Karen M</creatorcontrib><creatorcontrib>Chang, Caroline</creatorcontrib><creatorcontrib>Moore, Sharon P</creatorcontrib><collection>ProQuest Central (Corporate)</collection><collection>Docstoc</collection><collection>University Readers</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><jtitle>Genetics (Austin)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Garfinkel, David J</au><au>Nyswaner, Katherine M</au><au>Stefanisko, Karen M</au><au>Chang, Caroline</au><au>Moore, Sharon P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ty1 Copy Number Dynamics in Saccharomyces</atitle><jtitle>Genetics (Austin)</jtitle><date>2005-03-01</date><risdate>2005</risdate><volume>169</volume><issue>3</issue><spage>1845</spage><pages>1845-</pages><issn>0016-6731</issn><eissn>1943-2631</eissn><coden>GENTAE</coden><abstract>To understand long terminal repeat (LTR)-retrotransposon copy number dynamics, Ty1 elements were reintroduced into a "Ty-less" Saccharomyces strain where elements had been lost by LTR-LTR recombination. Repopulated strains exhibited alterations in chromosome size that were associated with Ty1 insertions, but did not become genetically isolated. The rates of element gain and loss under genetic and environmental conditions known to affect Ty1 retrotransposition were determined using genetically tagged reference elements. The results show that Ty1 retrotransposition varies with copy number, temperature, and cell type. In contrast to retrotransposition, Ty1 loss by LTR-LTR recombination was more constant and not markedly influenced by copy number. Endogenous Ty1 cDNA was poorly utilized for recombination when compared with LTR-LTR recombination or ectopic gene conversion. Ty1 elements also appear to be more susceptible to copy number fluctuation in haploid cells. Ty1 gain/loss ratios obtained under different conditions suggest that copy number oscillates over time by altering the rate of retrotransposition, resulting in the diverse copy numbers observed in Saccharomyces. [PUBLICATION ABSTRACT]</abstract><cop>Bethesda</cop><pub>Genetics Society of America</pub></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0016-6731 |
| ispartof | Genetics (Austin), 2005-03, Vol.169 (3), p.1845 |
| issn | 0016-6731 1943-2631 |
| language | eng |
| recordid | cdi_proquest_journals_214111916 |
| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Genetic recombination Genetics Genomics Microbiology Yeast |
| title | Ty1 Copy Number Dynamics in Saccharomyces |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T14%3A34%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Ty1%20Copy%20Number%20Dynamics%20in%20Saccharomyces&rft.jtitle=Genetics%20(Austin)&rft.au=Garfinkel,%20David%20J&rft.date=2005-03-01&rft.volume=169&rft.issue=3&rft.spage=1845&rft.pages=1845-&rft.issn=0016-6731&rft.eissn=1943-2631&rft.coden=GENTAE&rft_id=info:doi/&rft_dat=%3Cproquest%3E1036818271%3C/proquest%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=214111916&rft_id=info:pmid/&rfr_iscdi=true |