Exhaled breath condensate hydrogen peroxide, pH and leukotriene B4 are associated with lower airway inflammation and airway cytology in the horse
Summary Background Exhaled breath condensate (EBC) analysis is a noninvasive method to assess the lower respiratory tract. In human subjects, EBC hydrogen peroxide (H2O2), pH and leukotriene B4 (LTB4) are useful for detection and monitoring of inflammatory lung diseases, including asthma. Objectives...
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| Veröffentlicht in: | Equine veterinary journal 2019-01, Vol.51 (1), p.24-32 |
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| creator | du Preez, S. Raidal, S. L. Doran, G. S. Prescott, M. Hughes, K. J. |
| description | Summary
Background
Exhaled breath condensate (EBC) analysis is a noninvasive method to assess the lower respiratory tract. In human subjects, EBC hydrogen peroxide (H2O2), pH and leukotriene B4 (LTB4) are useful for detection and monitoring of inflammatory lung diseases, including asthma.
Objectives
To determine associations between EBC biomarkers and cytological and endoscopic definitions of lower airway inflammation (LAI) while controlling for sampling and environmental variables.
Study design
Prospective, cross‐sectional study.
Methods
Clinical, endoscopic and airway cytological findings from 47 horses were compared with EBC pH and concentrations of H2O2 and LTB4 by univariate and multivariable analyses. Dichotomous (presence/absence of airway inflammation) and continuous outcome variables (differential cell counts in tracheal aspirate and bronchoalveolar lavage fluid, BALF) were evaluated and potential effects of collection and methodological factors were included.
Results
EBC pH and H2O2 concentrations were higher in horses with LAI and both were positively associated with the percentage of neutrophils in BALF (P |
| doi_str_mv | 10.1111/evj.12979 |
| format | Article |
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Background
Exhaled breath condensate (EBC) analysis is a noninvasive method to assess the lower respiratory tract. In human subjects, EBC hydrogen peroxide (H2O2), pH and leukotriene B4 (LTB4) are useful for detection and monitoring of inflammatory lung diseases, including asthma.
Objectives
To determine associations between EBC biomarkers and cytological and endoscopic definitions of lower airway inflammation (LAI) while controlling for sampling and environmental variables.
Study design
Prospective, cross‐sectional study.
Methods
Clinical, endoscopic and airway cytological findings from 47 horses were compared with EBC pH and concentrations of H2O2 and LTB4 by univariate and multivariable analyses. Dichotomous (presence/absence of airway inflammation) and continuous outcome variables (differential cell counts in tracheal aspirate and bronchoalveolar lavage fluid, BALF) were evaluated and potential effects of collection and methodological factors were included.
Results
EBC pH and H2O2 concentrations were higher in horses with LAI and both were positively associated with the percentage of neutrophils in BALF (P<0.05). Mast cell percentage in BALF was negatively associated with EBC pH, and BALF eosinophil percentage was positively associated with EBC LTB4 (P<0.05). Ambient temperature, relative humidity and assay methodology significantly impacted some analytes.
Main limitations
LAI is challenging to categorise due to a variety of clinical and cytological phenotypes. Although the study was designed to overcome this limitation, numbers of horses were small in some categories.
Conclusions
EBC pH and H2O2 concentrations are altered by airway inflammation, suggesting a role for these biomarkers in the diagnosis and monitoring of airway disease. Environmental and methodological factors can influence these biomarkers and should be considered in the interpretation of results.</description><identifier>ISSN: 0425-1644</identifier><identifier>EISSN: 2042-3306</identifier><identifier>DOI: 10.1111/evj.12979</identifier><language>eng</language><publisher>Fordham: Wiley Subscription Services, Inc</publisher><subject>Alveoli ; Analytical chemistry ; asthma ; Biomarkers ; bronchoalveolar lavage ; Bronchus ; Condensates ; Cytology ; Endoscopy ; Environmental monitoring ; H2O2 ; horse ; Horses ; Hydrogen peroxide ; Inflammation ; inflammatory airway disease ; Leukocytes (neutrophilic) ; Leukotriene B4 ; LTB 4 ; Lung diseases ; Relative humidity ; Respiratory tract ; Respiratory tract diseases</subject><ispartof>Equine veterinary journal, 2019-01, Vol.51 (1), p.24-32</ispartof><rights>2018 EVJ Ltd</rights><rights>Copyright © 2019 EVJ Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0002-0167-9102</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fevj.12979$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fevj.12979$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids></links><search><creatorcontrib>du Preez, S.</creatorcontrib><creatorcontrib>Raidal, S. L.</creatorcontrib><creatorcontrib>Doran, G. S.</creatorcontrib><creatorcontrib>Prescott, M.</creatorcontrib><creatorcontrib>Hughes, K. J.</creatorcontrib><title>Exhaled breath condensate hydrogen peroxide, pH and leukotriene B4 are associated with lower airway inflammation and airway cytology in the horse</title><title>Equine veterinary journal</title><description>Summary
Background
Exhaled breath condensate (EBC) analysis is a noninvasive method to assess the lower respiratory tract. In human subjects, EBC hydrogen peroxide (H2O2), pH and leukotriene B4 (LTB4) are useful for detection and monitoring of inflammatory lung diseases, including asthma.
Objectives
To determine associations between EBC biomarkers and cytological and endoscopic definitions of lower airway inflammation (LAI) while controlling for sampling and environmental variables.
Study design
Prospective, cross‐sectional study.
Methods
Clinical, endoscopic and airway cytological findings from 47 horses were compared with EBC pH and concentrations of H2O2 and LTB4 by univariate and multivariable analyses. Dichotomous (presence/absence of airway inflammation) and continuous outcome variables (differential cell counts in tracheal aspirate and bronchoalveolar lavage fluid, BALF) were evaluated and potential effects of collection and methodological factors were included.
Results
EBC pH and H2O2 concentrations were higher in horses with LAI and both were positively associated with the percentage of neutrophils in BALF (P<0.05). Mast cell percentage in BALF was negatively associated with EBC pH, and BALF eosinophil percentage was positively associated with EBC LTB4 (P<0.05). Ambient temperature, relative humidity and assay methodology significantly impacted some analytes.
Main limitations
LAI is challenging to categorise due to a variety of clinical and cytological phenotypes. Although the study was designed to overcome this limitation, numbers of horses were small in some categories.
Conclusions
EBC pH and H2O2 concentrations are altered by airway inflammation, suggesting a role for these biomarkers in the diagnosis and monitoring of airway disease. Environmental and methodological factors can influence these biomarkers and should be considered in the interpretation of results.</description><subject>Alveoli</subject><subject>Analytical chemistry</subject><subject>asthma</subject><subject>Biomarkers</subject><subject>bronchoalveolar lavage</subject><subject>Bronchus</subject><subject>Condensates</subject><subject>Cytology</subject><subject>Endoscopy</subject><subject>Environmental monitoring</subject><subject>H2O2</subject><subject>horse</subject><subject>Horses</subject><subject>Hydrogen peroxide</subject><subject>Inflammation</subject><subject>inflammatory airway disease</subject><subject>Leukocytes (neutrophilic)</subject><subject>Leukotriene B4</subject><subject>LTB 4</subject><subject>Lung diseases</subject><subject>Relative humidity</subject><subject>Respiratory tract</subject><subject>Respiratory tract diseases</subject><issn>0425-1644</issn><issn>2042-3306</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNotUMtOwzAQtBBIlMKBP7DElbR-5NEcoSoUVIkLcI0ce9O4pHawU9J8Bn-MabuXHa1mZzSD0C0lExpmCj-bCWV5lp-hESMxizgn6TkaBZhENI3jS3Tl_YYQzlnMRuh3sa9FAwqXDkRXY2mNAuNFB7gelLNrMLgFZ_dawT1ul1gYhRvYfdnOaTCAH2MsHGDhvZU6vCnc66DT2B4cFtr1YsDaVI3YbkWnrTkInO5y6Gxj1_8E3NXB0ToP1-iiEo2Hm9Meo4-nxft8Ga3enl_mD6uopYTmUSJnvOQxUEGpLIGTJCmJohkVKcnLkK5kMpY8BxqzXJWVgipNJSFUZlWSMuBjdHfUbZ393oHvio3dORMsC0Z5nmR0lrHAmh5ZvW5gKFqnt8INBSXFf91FqLs41F0sPl8PgP8BWnp3HQ</recordid><startdate>201901</startdate><enddate>201901</enddate><creator>du Preez, S.</creator><creator>Raidal, S. L.</creator><creator>Doran, G. S.</creator><creator>Prescott, M.</creator><creator>Hughes, K. J.</creator><general>Wiley Subscription Services, Inc</general><scope>K9.</scope><orcidid>https://orcid.org/0000-0002-0167-9102</orcidid></search><sort><creationdate>201901</creationdate><title>Exhaled breath condensate hydrogen peroxide, pH and leukotriene B4 are associated with lower airway inflammation and airway cytology in the horse</title><author>du Preez, S. ; Raidal, S. L. ; Doran, G. S. ; Prescott, M. ; Hughes, K. J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p1019-5c83b34e1a11cbe3055b0d171a609b332b2c4c39e1429dbfdef66c001c7f562e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Alveoli</topic><topic>Analytical chemistry</topic><topic>asthma</topic><topic>Biomarkers</topic><topic>bronchoalveolar lavage</topic><topic>Bronchus</topic><topic>Condensates</topic><topic>Cytology</topic><topic>Endoscopy</topic><topic>Environmental monitoring</topic><topic>H2O2</topic><topic>horse</topic><topic>Horses</topic><topic>Hydrogen peroxide</topic><topic>Inflammation</topic><topic>inflammatory airway disease</topic><topic>Leukocytes (neutrophilic)</topic><topic>Leukotriene B4</topic><topic>LTB 4</topic><topic>Lung diseases</topic><topic>Relative humidity</topic><topic>Respiratory tract</topic><topic>Respiratory tract diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>du Preez, S.</creatorcontrib><creatorcontrib>Raidal, S. L.</creatorcontrib><creatorcontrib>Doran, G. S.</creatorcontrib><creatorcontrib>Prescott, M.</creatorcontrib><creatorcontrib>Hughes, K. J.</creatorcontrib><collection>ProQuest Health & Medical Complete (Alumni)</collection><jtitle>Equine veterinary journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>du Preez, S.</au><au>Raidal, S. L.</au><au>Doran, G. S.</au><au>Prescott, M.</au><au>Hughes, K. J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Exhaled breath condensate hydrogen peroxide, pH and leukotriene B4 are associated with lower airway inflammation and airway cytology in the horse</atitle><jtitle>Equine veterinary journal</jtitle><date>2019-01</date><risdate>2019</risdate><volume>51</volume><issue>1</issue><spage>24</spage><epage>32</epage><pages>24-32</pages><issn>0425-1644</issn><eissn>2042-3306</eissn><abstract>Summary
Background
Exhaled breath condensate (EBC) analysis is a noninvasive method to assess the lower respiratory tract. In human subjects, EBC hydrogen peroxide (H2O2), pH and leukotriene B4 (LTB4) are useful for detection and monitoring of inflammatory lung diseases, including asthma.
Objectives
To determine associations between EBC biomarkers and cytological and endoscopic definitions of lower airway inflammation (LAI) while controlling for sampling and environmental variables.
Study design
Prospective, cross‐sectional study.
Methods
Clinical, endoscopic and airway cytological findings from 47 horses were compared with EBC pH and concentrations of H2O2 and LTB4 by univariate and multivariable analyses. Dichotomous (presence/absence of airway inflammation) and continuous outcome variables (differential cell counts in tracheal aspirate and bronchoalveolar lavage fluid, BALF) were evaluated and potential effects of collection and methodological factors were included.
Results
EBC pH and H2O2 concentrations were higher in horses with LAI and both were positively associated with the percentage of neutrophils in BALF (P<0.05). Mast cell percentage in BALF was negatively associated with EBC pH, and BALF eosinophil percentage was positively associated with EBC LTB4 (P<0.05). Ambient temperature, relative humidity and assay methodology significantly impacted some analytes.
Main limitations
LAI is challenging to categorise due to a variety of clinical and cytological phenotypes. Although the study was designed to overcome this limitation, numbers of horses were small in some categories.
Conclusions
EBC pH and H2O2 concentrations are altered by airway inflammation, suggesting a role for these biomarkers in the diagnosis and monitoring of airway disease. Environmental and methodological factors can influence these biomarkers and should be considered in the interpretation of results.</abstract><cop>Fordham</cop><pub>Wiley Subscription Services, Inc</pub><doi>10.1111/evj.12979</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-0167-9102</orcidid></addata></record> |
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| subjects | Alveoli Analytical chemistry asthma Biomarkers bronchoalveolar lavage Bronchus Condensates Cytology Endoscopy Environmental monitoring H2O2 horse Horses Hydrogen peroxide Inflammation inflammatory airway disease Leukocytes (neutrophilic) Leukotriene B4 LTB 4 Lung diseases Relative humidity Respiratory tract Respiratory tract diseases |
| title | Exhaled breath condensate hydrogen peroxide, pH and leukotriene B4 are associated with lower airway inflammation and airway cytology in the horse |
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