Red cell supernatant effects on endothelial cell function and innate immune activation is influenced by donor age and sex
Background and objectives Blood donor characteristics significantly affect the pre‐storage and post‐storage quality of red cell concentrates (RCCs). This study investigated the impact of donor age and sex on RCC characteristics and the effect of RCC supernatants on the vascular endothelium and monoc...
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| Veröffentlicht in: | ISBT science series 2018-12, Vol.13 (4), p.446-459 |
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| creator | Kipkeu, Betty J. Almizraq, Ruqayyah Branch, Donald R. Acker, Jason P. Holovati, Jelena L. |
| description | Background and objectives
Blood donor characteristics significantly affect the pre‐storage and post‐storage quality of red cell concentrates (RCCs). This study investigated the impact of donor age and sex on RCC characteristics and the effect of RCC supernatants on the vascular endothelium and monocytes.
Materials and methods
RCC units selected from four donor groups based on donor age and sex (male ≤ 30‐years old (yo), male ≥ 60‐yo, female ≤ 30‐yo and female ≥ 60‐yo) were stored at 1–6°C and tested on day 7, 21 and 42. RCC supernatants were analysed for the number, concentration and size of extracellular vesicles (EVs). Human vein endothelial cells (HUVECs) monolayers were incubated with RCC supernatants and assessed for expression of vascular cell adhesion molecule‐1 (VCAM‐1) and E‐selectin by flow cytometry as well as endothelial permeability using a trans‐epithelial‐electrical‐resistance assay. Monocytes and HUVECs were incubated with RCC supernatants and supernatants assayed for cytokines/chemokines.
Results
RCCs from female donors were found to have lower unit volume, haemoglobin content and haematocrit compared to RCCs from male donors (P |
| doi_str_mv | 10.1111/voxs.12472 |
| format | Article |
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Blood donor characteristics significantly affect the pre‐storage and post‐storage quality of red cell concentrates (RCCs). This study investigated the impact of donor age and sex on RCC characteristics and the effect of RCC supernatants on the vascular endothelium and monocytes.
Materials and methods
RCC units selected from four donor groups based on donor age and sex (male ≤ 30‐years old (yo), male ≥ 60‐yo, female ≤ 30‐yo and female ≥ 60‐yo) were stored at 1–6°C and tested on day 7, 21 and 42. RCC supernatants were analysed for the number, concentration and size of extracellular vesicles (EVs). Human vein endothelial cells (HUVECs) monolayers were incubated with RCC supernatants and assessed for expression of vascular cell adhesion molecule‐1 (VCAM‐1) and E‐selectin by flow cytometry as well as endothelial permeability using a trans‐epithelial‐electrical‐resistance assay. Monocytes and HUVECs were incubated with RCC supernatants and supernatants assayed for cytokines/chemokines.
Results
RCCs from female donors were found to have lower unit volume, haemoglobin content and haematocrit compared to RCCs from male donors (P < 0·05). RCC supernatants from female ≥ 60‐yo had a higher concentration of large EVs (≥200 nm) compared to both male ≤ 30‐yo (P = 0·039) and male ≥ 60‐yo supernatants (P = 0·019). Treatment with RCC supernatants from male ≤ 30‐yo donors induced lower expression of VCAM‐1, lower cytokine/chemokine release and showed the least effect on endothelial permeability.
Conclusion
Donor characteristics affect RCC quality parameters and influence the endothelial and immune modulation potential of RCC supernatants in vitro.</description><identifier>ISSN: 1751-2816</identifier><identifier>EISSN: 1751-2824</identifier><identifier>DOI: 10.1111/voxs.12472</identifier><language>eng</language><publisher>Oxford: Wiley Subscription Services, Inc</publisher><subject>age ; Blood & organ donations ; blood donor ; Cell adhesion & migration ; Endothelium ; Erythrocytes ; Permeability ; red cell supernatants ; sex ; transfusion‐related immune modulation</subject><ispartof>ISBT science series, 2018-12, Vol.13 (4), p.446-459</ispartof><rights>2018 International Society of Blood Transfusion</rights><rights>Copyright ISBT Science Series © 2018 International Society of Blood Transfusion</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1462-3c893b07013528012baa4fccfcf2cebb381147b511e55a6ca2c545abf3fab1aa3</citedby><cites>FETCH-LOGICAL-c1462-3c893b07013528012baa4fccfcf2cebb381147b511e55a6ca2c545abf3fab1aa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fvoxs.12472$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fvoxs.12472$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids></links><search><creatorcontrib>Kipkeu, Betty J.</creatorcontrib><creatorcontrib>Almizraq, Ruqayyah</creatorcontrib><creatorcontrib>Branch, Donald R.</creatorcontrib><creatorcontrib>Acker, Jason P.</creatorcontrib><creatorcontrib>Holovati, Jelena L.</creatorcontrib><title>Red cell supernatant effects on endothelial cell function and innate immune activation is influenced by donor age and sex</title><title>ISBT science series</title><description>Background and objectives
Blood donor characteristics significantly affect the pre‐storage and post‐storage quality of red cell concentrates (RCCs). This study investigated the impact of donor age and sex on RCC characteristics and the effect of RCC supernatants on the vascular endothelium and monocytes.
Materials and methods
RCC units selected from four donor groups based on donor age and sex (male ≤ 30‐years old (yo), male ≥ 60‐yo, female ≤ 30‐yo and female ≥ 60‐yo) were stored at 1–6°C and tested on day 7, 21 and 42. RCC supernatants were analysed for the number, concentration and size of extracellular vesicles (EVs). Human vein endothelial cells (HUVECs) monolayers were incubated with RCC supernatants and assessed for expression of vascular cell adhesion molecule‐1 (VCAM‐1) and E‐selectin by flow cytometry as well as endothelial permeability using a trans‐epithelial‐electrical‐resistance assay. Monocytes and HUVECs were incubated with RCC supernatants and supernatants assayed for cytokines/chemokines.
Results
RCCs from female donors were found to have lower unit volume, haemoglobin content and haematocrit compared to RCCs from male donors (P < 0·05). RCC supernatants from female ≥ 60‐yo had a higher concentration of large EVs (≥200 nm) compared to both male ≤ 30‐yo (P = 0·039) and male ≥ 60‐yo supernatants (P = 0·019). Treatment with RCC supernatants from male ≤ 30‐yo donors induced lower expression of VCAM‐1, lower cytokine/chemokine release and showed the least effect on endothelial permeability.
Conclusion
Donor characteristics affect RCC quality parameters and influence the endothelial and immune modulation potential of RCC supernatants in vitro.</description><subject>age</subject><subject>Blood & organ donations</subject><subject>blood donor</subject><subject>Cell adhesion & migration</subject><subject>Endothelium</subject><subject>Erythrocytes</subject><subject>Permeability</subject><subject>red cell supernatants</subject><subject>sex</subject><subject>transfusion‐related immune modulation</subject><issn>1751-2816</issn><issn>1751-2824</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp9kFtLAzEQhYMoWKsv_oKAb0LrTvYWH6V4g0LBG74tk-xEt6TZutmt3X9v2hUfnZcZON-ZYQ5j5xBNIdTVpt76KYgkFwdsBHkKEyFFcvg3Q3bMTrxfRlGayDwZsf6JSq7JWu67NTUOW3QtJ2NIt57XjpMr6_aTbIV24EzndFsFBV3JKxccxKvVqnPEMQgb3IuVD5qxHTkdDqiel7WrG44ftPd52p6yI4PW09lvH7PXu9uX2cNkvrh_nN3MJxqSTExiLa9jFeURxKmQEQiFmBitjTZCk1KxBEhylQJQmmKmUeg0SVGZ2KACxHjMLoa966b-6si3xbLuwqPWFwJiKbMYpAjU5UDppva-IVOsm2qFTV9AVOyiLXbRFvtoAwwD_F1Z6v8hi7fF-_Pg-QEMAH5p</recordid><startdate>201812</startdate><enddate>201812</enddate><creator>Kipkeu, Betty J.</creator><creator>Almizraq, Ruqayyah</creator><creator>Branch, Donald R.</creator><creator>Acker, Jason P.</creator><creator>Holovati, Jelena L.</creator><general>Wiley Subscription Services, Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope></search><sort><creationdate>201812</creationdate><title>Red cell supernatant effects on endothelial cell function and innate immune activation is influenced by donor age and sex</title><author>Kipkeu, Betty J. ; Almizraq, Ruqayyah ; Branch, Donald R. ; Acker, Jason P. ; Holovati, Jelena L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1462-3c893b07013528012baa4fccfcf2cebb381147b511e55a6ca2c545abf3fab1aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>age</topic><topic>Blood & organ donations</topic><topic>blood donor</topic><topic>Cell adhesion & migration</topic><topic>Endothelium</topic><topic>Erythrocytes</topic><topic>Permeability</topic><topic>red cell supernatants</topic><topic>sex</topic><topic>transfusion‐related immune modulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kipkeu, Betty J.</creatorcontrib><creatorcontrib>Almizraq, Ruqayyah</creatorcontrib><creatorcontrib>Branch, Donald R.</creatorcontrib><creatorcontrib>Acker, Jason P.</creatorcontrib><creatorcontrib>Holovati, Jelena L.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><jtitle>ISBT science series</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kipkeu, Betty J.</au><au>Almizraq, Ruqayyah</au><au>Branch, Donald R.</au><au>Acker, Jason P.</au><au>Holovati, Jelena L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Red cell supernatant effects on endothelial cell function and innate immune activation is influenced by donor age and sex</atitle><jtitle>ISBT science series</jtitle><date>2018-12</date><risdate>2018</risdate><volume>13</volume><issue>4</issue><spage>446</spage><epage>459</epage><pages>446-459</pages><issn>1751-2816</issn><eissn>1751-2824</eissn><abstract>Background and objectives
Blood donor characteristics significantly affect the pre‐storage and post‐storage quality of red cell concentrates (RCCs). This study investigated the impact of donor age and sex on RCC characteristics and the effect of RCC supernatants on the vascular endothelium and monocytes.
Materials and methods
RCC units selected from four donor groups based on donor age and sex (male ≤ 30‐years old (yo), male ≥ 60‐yo, female ≤ 30‐yo and female ≥ 60‐yo) were stored at 1–6°C and tested on day 7, 21 and 42. RCC supernatants were analysed for the number, concentration and size of extracellular vesicles (EVs). Human vein endothelial cells (HUVECs) monolayers were incubated with RCC supernatants and assessed for expression of vascular cell adhesion molecule‐1 (VCAM‐1) and E‐selectin by flow cytometry as well as endothelial permeability using a trans‐epithelial‐electrical‐resistance assay. Monocytes and HUVECs were incubated with RCC supernatants and supernatants assayed for cytokines/chemokines.
Results
RCCs from female donors were found to have lower unit volume, haemoglobin content and haematocrit compared to RCCs from male donors (P < 0·05). RCC supernatants from female ≥ 60‐yo had a higher concentration of large EVs (≥200 nm) compared to both male ≤ 30‐yo (P = 0·039) and male ≥ 60‐yo supernatants (P = 0·019). Treatment with RCC supernatants from male ≤ 30‐yo donors induced lower expression of VCAM‐1, lower cytokine/chemokine release and showed the least effect on endothelial permeability.
Conclusion
Donor characteristics affect RCC quality parameters and influence the endothelial and immune modulation potential of RCC supernatants in vitro.</abstract><cop>Oxford</cop><pub>Wiley Subscription Services, Inc</pub><doi>10.1111/voxs.12472</doi><tpages>14</tpages></addata></record> |
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| ispartof | ISBT science series, 2018-12, Vol.13 (4), p.446-459 |
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| language | eng |
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| source | Wiley Journals |
| subjects | age Blood & organ donations blood donor Cell adhesion & migration Endothelium Erythrocytes Permeability red cell supernatants sex transfusion‐related immune modulation |
| title | Red cell supernatant effects on endothelial cell function and innate immune activation is influenced by donor age and sex |
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