In Vitro Cultivation of Theileria lawrencei-Intected Lymphoblastoid Cell Lines Derived from a Buffalo (Syncerus caffer)
A buffalo calf was obtained from a Theileria lawrencei enzootic area of Kenya and found to be carrying intra-erythrocytic theilerial piroplasms. Leucocytes were harvested from this buffalo by sedimentation of heparinized blood and cultured on bovine embryo spleen (BESP) feeder layers. After 18 to 42...
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| Veröffentlicht in: | Research in veterinary science 1974-01, Vol.16 (1), p.125-127 |
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| creator | Stagg, D.A. Brown, C.G.D. Crawford, J.G. Kanhai, G.K. Young, A.S. |
| description | A buffalo calf was obtained from a Theileria lawrencei enzootic area of Kenya and found to be carrying intra-erythrocytic theilerial piroplasms. Leucocytes were harvested from this buffalo by sedimentation of heparinized blood and cultured on bovine embryo spleen (BESP) feeder layers. After 18 to 42 days in culture, transformation occurred and the cultures gave rise to cell lines of lymphoblastoid cells injected with T. lawrencei macroschizonts. On chromosomal analysis, the infected cells were shown to be of buffalo origin. Eight separate isolates were made from the buffalo over a 5-month period. Similar cultures of buffalo leucocytes without a BESP feeder layer failed to transform. |
| doi_str_mv | 10.1016/S0034-5288(18)33792-5 |
| format | Article |
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Leucocytes were harvested from this buffalo by sedimentation of heparinized blood and cultured on bovine embryo spleen (BESP) feeder layers. After 18 to 42 days in culture, transformation occurred and the cultures gave rise to cell lines of lymphoblastoid cells injected with T. lawrencei macroschizonts. On chromosomal analysis, the infected cells were shown to be of buffalo origin. Eight separate isolates were made from the buffalo over a 5-month period. Similar cultures of buffalo leucocytes without a BESP feeder layer failed to transform.</description><identifier>ISSN: 0034-5288</identifier><identifier>EISSN: 1532-2661</identifier><identifier>DOI: 10.1016/S0034-5288(18)33792-5</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Biotechnology ; Cell culture ; Cultivation ; Embryos ; Erythrocyte sedimentation rate ; Genetic transformation ; Leukocytes ; Lymphoblastoid cell lines ; Sedimentation ; Spleen ; Theileria ; Veterinary medicine</subject><ispartof>Research in veterinary science, 1974-01, Vol.16 (1), p.125-127</ispartof><rights>1974</rights><rights>Copyright Elsevier Limited Jan 1974</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1821-d19c3d2aa4900b671c99c6151999a9de319fd2f7cc02ff225e81d5bfdc2af7533</citedby><cites>FETCH-LOGICAL-c1821-d19c3d2aa4900b671c99c6151999a9de319fd2f7cc02ff225e81d5bfdc2af7533</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0034-5288(18)33792-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3541,27915,27916,45986</link.rule.ids></links><search><creatorcontrib>Stagg, D.A.</creatorcontrib><creatorcontrib>Brown, C.G.D.</creatorcontrib><creatorcontrib>Crawford, J.G.</creatorcontrib><creatorcontrib>Kanhai, G.K.</creatorcontrib><creatorcontrib>Young, A.S.</creatorcontrib><title>In Vitro Cultivation of Theileria lawrencei-Intected Lymphoblastoid Cell Lines Derived from a Buffalo (Syncerus caffer)</title><title>Research in veterinary science</title><description>A buffalo calf was obtained from a Theileria lawrencei enzootic area of Kenya and found to be carrying intra-erythrocytic theilerial piroplasms. Leucocytes were harvested from this buffalo by sedimentation of heparinized blood and cultured on bovine embryo spleen (BESP) feeder layers. After 18 to 42 days in culture, transformation occurred and the cultures gave rise to cell lines of lymphoblastoid cells injected with T. lawrencei macroschizonts. On chromosomal analysis, the infected cells were shown to be of buffalo origin. Eight separate isolates were made from the buffalo over a 5-month period. Similar cultures of buffalo leucocytes without a BESP feeder layer failed to transform.</description><subject>Biotechnology</subject><subject>Cell culture</subject><subject>Cultivation</subject><subject>Embryos</subject><subject>Erythrocyte sedimentation rate</subject><subject>Genetic transformation</subject><subject>Leukocytes</subject><subject>Lymphoblastoid cell lines</subject><subject>Sedimentation</subject><subject>Spleen</subject><subject>Theileria</subject><subject>Veterinary medicine</subject><issn>0034-5288</issn><issn>1532-2661</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1974</creationdate><recordtype>article</recordtype><recordid>eNqFkMtKAzEUhoMoWC-PIATc6GI0J2lmJivReisUXLS6DWkuNGU6qclMpW9vasWtq7M43_8fzofQBZAbIFDeTglhw4LTur6C-pqxStCCH6ABcEYLWpZwiAZ_yDE6SWlJCBkCVAP0NW7xh-9iwKO-6fxGdT60ODg8W1jf2OgVbtRXtK22vhi3ndWdNXiyXa0XYd6o1AVv8Mg2DZ741ib8mCObTLgYVljhh9451QR8Nd3mhtgnrJVzNl6foaO8SPb8d56i9-en2ei1mLy9jEf3k0JDTaEwIDQzVKmhIGReVqCF0CVwEEIoYSwD4Qx1ldaEOkcptzUYPndGU-Uqztgputz3rmP47G3q5DL0sc0nJQVW1VVJh5Apvqd0DClF6-Q6-pWKWwlE7hzLH8dyJ1BCLX8cS55zd_uczS9svI0yab9zZXzMpqQJ_p-Gb2lGhCw</recordid><startdate>197401</startdate><enddate>197401</enddate><creator>Stagg, D.A.</creator><creator>Brown, C.G.D.</creator><creator>Crawford, J.G.</creator><creator>Kanhai, G.K.</creator><creator>Young, A.S.</creator><general>Elsevier Ltd</general><general>Elsevier Limited</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>197401</creationdate><title>In Vitro Cultivation of Theileria lawrencei-Intected Lymphoblastoid Cell Lines Derived from a Buffalo (Syncerus caffer)</title><author>Stagg, D.A. ; Brown, C.G.D. ; Crawford, J.G. ; Kanhai, G.K. ; Young, A.S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1821-d19c3d2aa4900b671c99c6151999a9de319fd2f7cc02ff225e81d5bfdc2af7533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1974</creationdate><topic>Biotechnology</topic><topic>Cell culture</topic><topic>Cultivation</topic><topic>Embryos</topic><topic>Erythrocyte sedimentation rate</topic><topic>Genetic transformation</topic><topic>Leukocytes</topic><topic>Lymphoblastoid cell lines</topic><topic>Sedimentation</topic><topic>Spleen</topic><topic>Theileria</topic><topic>Veterinary medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stagg, D.A.</creatorcontrib><creatorcontrib>Brown, C.G.D.</creatorcontrib><creatorcontrib>Crawford, J.G.</creatorcontrib><creatorcontrib>Kanhai, G.K.</creatorcontrib><creatorcontrib>Young, A.S.</creatorcontrib><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Research in veterinary science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stagg, D.A.</au><au>Brown, C.G.D.</au><au>Crawford, J.G.</au><au>Kanhai, G.K.</au><au>Young, A.S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In Vitro Cultivation of Theileria lawrencei-Intected Lymphoblastoid Cell Lines Derived from a Buffalo (Syncerus caffer)</atitle><jtitle>Research in veterinary science</jtitle><date>1974-01</date><risdate>1974</risdate><volume>16</volume><issue>1</issue><spage>125</spage><epage>127</epage><pages>125-127</pages><issn>0034-5288</issn><eissn>1532-2661</eissn><abstract>A buffalo calf was obtained from a Theileria lawrencei enzootic area of Kenya and found to be carrying intra-erythrocytic theilerial piroplasms. Leucocytes were harvested from this buffalo by sedimentation of heparinized blood and cultured on bovine embryo spleen (BESP) feeder layers. After 18 to 42 days in culture, transformation occurred and the cultures gave rise to cell lines of lymphoblastoid cells injected with T. lawrencei macroschizonts. On chromosomal analysis, the infected cells were shown to be of buffalo origin. Eight separate isolates were made from the buffalo over a 5-month period. Similar cultures of buffalo leucocytes without a BESP feeder layer failed to transform.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><doi>10.1016/S0034-5288(18)33792-5</doi><tpages>3</tpages></addata></record> |
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| identifier | ISSN: 0034-5288 |
| ispartof | Research in veterinary science, 1974-01, Vol.16 (1), p.125-127 |
| issn | 0034-5288 1532-2661 |
| language | eng |
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| source | Elsevier ScienceDirect Journals Complete |
| subjects | Biotechnology Cell culture Cultivation Embryos Erythrocyte sedimentation rate Genetic transformation Leukocytes Lymphoblastoid cell lines Sedimentation Spleen Theileria Veterinary medicine |
| title | In Vitro Cultivation of Theileria lawrencei-Intected Lymphoblastoid Cell Lines Derived from a Buffalo (Syncerus caffer) |
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