Optimal conditions for freezing CHO-S and HEK293-EBNA cell lines: Influence of Me^sub 2^SO, freeze density, and PEI-mediated transfection on revitalization and growth of cells, and expression of recombinant protein

To avoid the time consuming, labor intensive seed-train expansion and to improve production reliability and consistency, portions of bulk cryopreserved cells from the same cultivation can be utilized as inocula or alternatively may be used to undertake transient transfections for large-scale bioreac...

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Veröffentlicht in:	Biotechnology and bioengineering 2008-08, Vol.100 (5), p.911
Hauptverfasser:	Kleman, Marika I, Oellers, Kerstin, Lullau, Elke
Format:	Artikel
Sprache:	eng
Schlagworte:	Cell culture Cell growth Freezing Gene expression Optimization Proteins Studies
Online-Zugang:	Volltext
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