Thy-1 is an in vivo and in vitro marker of liver myofibroblasts
Thy-1, a glycophosphatidylinositol-linked glycoprotein of the outer membrane leaflet, has been described in myofibroblasts of several organs. Previous studies have shown that, in fetal liver, Thy-1 is expressed in a subpopulation of ductular/progenitor cells. The aim of this study has been to invest...
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| Veröffentlicht in: | Cell and tissue research 2007-09, Vol.329 (3), p.503-514 |
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| creator | Dudas, Jozsef Mansuroglu, Tümen Batusic, Danko Saile, Bernhard Ramadori, Giuliano |
| description | Thy-1, a glycophosphatidylinositol-linked glycoprotein of the outer membrane leaflet, has been described in myofibroblasts of several organs. Previous studies have shown that, in fetal liver, Thy-1 is expressed in a subpopulation of ductular/progenitor cells. The aim of this study has been to investigate whether the liver myofibroblasts belong to the Thy-1-positive subpopulation of the adult liver. The expression of Thy-1 has been studied in normal rat liver, in the rat liver regeneration model following 2-acetylaminofluorene treatment and partial hepatectomy (AAF/PH), and in isolated rat liver cells, at the mRNA and protein levels. In normal rat liver, Thy-1 is detected in sparse cells of the periportal area, whereas 7 days after PH in the AAF/PH model, a marked increase of the number of Thy-1-positive cells is detectable by immunohistochemistry. Comparative immunohistochemical analysis has revealed the co-localization of Thy-1 and smooth muscle actin, but not of Thy-1 and cytokeratin-19, both in normal rat liver and in the AAF/PH model. Investigation of isolated rat liver cell populations has confirmed that liver myofibroblasts are Thy-1-positive cells, whereas hepatocytes, hepatic stellate cells, and liver macrophages are not. Thy-1 is the first cell surface marker for identifying liver myofibroblasts in vivo and in vitro. |
| doi_str_mv | 10.1007/s00441-007-0437-z |
| format | Article |
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Previous studies have shown that, in fetal liver, Thy-1 is expressed in a subpopulation of ductular/progenitor cells. The aim of this study has been to investigate whether the liver myofibroblasts belong to the Thy-1-positive subpopulation of the adult liver. The expression of Thy-1 has been studied in normal rat liver, in the rat liver regeneration model following 2-acetylaminofluorene treatment and partial hepatectomy (AAF/PH), and in isolated rat liver cells, at the mRNA and protein levels. In normal rat liver, Thy-1 is detected in sparse cells of the periportal area, whereas 7 days after PH in the AAF/PH model, a marked increase of the number of Thy-1-positive cells is detectable by immunohistochemistry. Comparative immunohistochemical analysis has revealed the co-localization of Thy-1 and smooth muscle actin, but not of Thy-1 and cytokeratin-19, both in normal rat liver and in the AAF/PH model. Investigation of isolated rat liver cell populations has confirmed that liver myofibroblasts are Thy-1-positive cells, whereas hepatocytes, hepatic stellate cells, and liver macrophages are not. Thy-1 is the first cell surface marker for identifying liver myofibroblasts in vivo and in vitro.</description><identifier>ISSN: 0302-766X</identifier><identifier>EISSN: 1432-0878</identifier><identifier>DOI: 10.1007/s00441-007-0437-z</identifier><identifier>PMID: 17576600</identifier><language>eng</language><publisher>Germany: Springer Nature B.V</publisher><subject>2-Acetylaminofluorene - pharmacology ; Animals ; Biomarkers - metabolism ; Biomedical research ; Cell Separation ; Cellular biology ; Fibroblasts - cytology ; Fibroblasts - metabolism ; Gene Expression Regulation ; Glycoproteins ; Hepatectomy ; Hepatocytes - cytology ; Hepatocytes - metabolism ; Liver ; Liver - cytology ; Liver - metabolism ; Liver - surgery ; Liver Regeneration ; Rats ; Rats, Inbred F344 ; Ribonucleic acid ; RNA ; Rodents ; Thy-1 Antigens - analysis ; Thy-1 Antigens - genetics ; Thy-1 Antigens - metabolism</subject><ispartof>Cell and tissue research, 2007-09, Vol.329 (3), p.503-514</ispartof><rights>Springer-Verlag 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c326t-5d7d193f9bb8963d02dd22681e6aef1ff2a9432e5350208443f4a0e2cbca3bdf3</citedby><cites>FETCH-LOGICAL-c326t-5d7d193f9bb8963d02dd22681e6aef1ff2a9432e5350208443f4a0e2cbca3bdf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17576600$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dudas, Jozsef</creatorcontrib><creatorcontrib>Mansuroglu, Tümen</creatorcontrib><creatorcontrib>Batusic, Danko</creatorcontrib><creatorcontrib>Saile, Bernhard</creatorcontrib><creatorcontrib>Ramadori, Giuliano</creatorcontrib><title>Thy-1 is an in vivo and in vitro marker of liver myofibroblasts</title><title>Cell and tissue research</title><addtitle>Cell Tissue Res</addtitle><description>Thy-1, a glycophosphatidylinositol-linked glycoprotein of the outer membrane leaflet, has been described in myofibroblasts of several organs. Previous studies have shown that, in fetal liver, Thy-1 is expressed in a subpopulation of ductular/progenitor cells. The aim of this study has been to investigate whether the liver myofibroblasts belong to the Thy-1-positive subpopulation of the adult liver. The expression of Thy-1 has been studied in normal rat liver, in the rat liver regeneration model following 2-acetylaminofluorene treatment and partial hepatectomy (AAF/PH), and in isolated rat liver cells, at the mRNA and protein levels. In normal rat liver, Thy-1 is detected in sparse cells of the periportal area, whereas 7 days after PH in the AAF/PH model, a marked increase of the number of Thy-1-positive cells is detectable by immunohistochemistry. Comparative immunohistochemical analysis has revealed the co-localization of Thy-1 and smooth muscle actin, but not of Thy-1 and cytokeratin-19, both in normal rat liver and in the AAF/PH model. Investigation of isolated rat liver cell populations has confirmed that liver myofibroblasts are Thy-1-positive cells, whereas hepatocytes, hepatic stellate cells, and liver macrophages are not. Thy-1 is the first cell surface marker for identifying liver myofibroblasts in vivo and in vitro.</description><subject>2-Acetylaminofluorene - pharmacology</subject><subject>Animals</subject><subject>Biomarkers - metabolism</subject><subject>Biomedical research</subject><subject>Cell Separation</subject><subject>Cellular biology</subject><subject>Fibroblasts - cytology</subject><subject>Fibroblasts - metabolism</subject><subject>Gene Expression Regulation</subject><subject>Glycoproteins</subject><subject>Hepatectomy</subject><subject>Hepatocytes - cytology</subject><subject>Hepatocytes - metabolism</subject><subject>Liver</subject><subject>Liver - cytology</subject><subject>Liver - metabolism</subject><subject>Liver - surgery</subject><subject>Liver Regeneration</subject><subject>Rats</subject><subject>Rats, Inbred F344</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Rodents</subject><subject>Thy-1 Antigens - analysis</subject><subject>Thy-1 Antigens - genetics</subject><subject>Thy-1 Antigens - 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pharmacology</topic><topic>Animals</topic><topic>Biomarkers - metabolism</topic><topic>Biomedical research</topic><topic>Cell Separation</topic><topic>Cellular biology</topic><topic>Fibroblasts - cytology</topic><topic>Fibroblasts - metabolism</topic><topic>Gene Expression Regulation</topic><topic>Glycoproteins</topic><topic>Hepatectomy</topic><topic>Hepatocytes - cytology</topic><topic>Hepatocytes - metabolism</topic><topic>Liver</topic><topic>Liver - cytology</topic><topic>Liver - metabolism</topic><topic>Liver - surgery</topic><topic>Liver Regeneration</topic><topic>Rats</topic><topic>Rats, Inbred F344</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Rodents</topic><topic>Thy-1 Antigens - analysis</topic><topic>Thy-1 Antigens - genetics</topic><topic>Thy-1 Antigens - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dudas, Jozsef</creatorcontrib><creatorcontrib>Mansuroglu, Tümen</creatorcontrib><creatorcontrib>Batusic, Danko</creatorcontrib><creatorcontrib>Saile, Bernhard</creatorcontrib><creatorcontrib>Ramadori, Giuliano</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><jtitle>Cell and tissue research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dudas, Jozsef</au><au>Mansuroglu, Tümen</au><au>Batusic, Danko</au><au>Saile, Bernhard</au><au>Ramadori, Giuliano</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Thy-1 is an in vivo and in vitro marker of liver myofibroblasts</atitle><jtitle>Cell and tissue research</jtitle><addtitle>Cell Tissue Res</addtitle><date>2007-09-01</date><risdate>2007</risdate><volume>329</volume><issue>3</issue><spage>503</spage><epage>514</epage><pages>503-514</pages><issn>0302-766X</issn><eissn>1432-0878</eissn><abstract>Thy-1, a glycophosphatidylinositol-linked glycoprotein of the outer membrane leaflet, has been described in myofibroblasts of several organs. Previous studies have shown that, in fetal liver, Thy-1 is expressed in a subpopulation of ductular/progenitor cells. The aim of this study has been to investigate whether the liver myofibroblasts belong to the Thy-1-positive subpopulation of the adult liver. The expression of Thy-1 has been studied in normal rat liver, in the rat liver regeneration model following 2-acetylaminofluorene treatment and partial hepatectomy (AAF/PH), and in isolated rat liver cells, at the mRNA and protein levels. In normal rat liver, Thy-1 is detected in sparse cells of the periportal area, whereas 7 days after PH in the AAF/PH model, a marked increase of the number of Thy-1-positive cells is detectable by immunohistochemistry. Comparative immunohistochemical analysis has revealed the co-localization of Thy-1 and smooth muscle actin, but not of Thy-1 and cytokeratin-19, both in normal rat liver and in the AAF/PH model. Investigation of isolated rat liver cell populations has confirmed that liver myofibroblasts are Thy-1-positive cells, whereas hepatocytes, hepatic stellate cells, and liver macrophages are not. Thy-1 is the first cell surface marker for identifying liver myofibroblasts in vivo and in vitro.</abstract><cop>Germany</cop><pub>Springer Nature B.V</pub><pmid>17576600</pmid><doi>10.1007/s00441-007-0437-z</doi><tpages>12</tpages></addata></record> |
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| subjects | 2-Acetylaminofluorene - pharmacology Animals Biomarkers - metabolism Biomedical research Cell Separation Cellular biology Fibroblasts - cytology Fibroblasts - metabolism Gene Expression Regulation Glycoproteins Hepatectomy Hepatocytes - cytology Hepatocytes - metabolism Liver Liver - cytology Liver - metabolism Liver - surgery Liver Regeneration Rats Rats, Inbred F344 Ribonucleic acid RNA Rodents Thy-1 Antigens - analysis Thy-1 Antigens - genetics Thy-1 Antigens - metabolism |
| title | Thy-1 is an in vivo and in vitro marker of liver myofibroblasts |
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