Prussian Blue-Based Thin-Layer Flow-Injection Multibiosensor for Simultaneous Determination of Glucose and Lactate
A planar multibiosensor for the simultaneous determination of glucose and lactate is developed by combining the Prussian Blue-based electrocatalyst and the protocol for immobilization of glucose oxidase and lactate oxidase enzymes from solutions with a high content of an organic solvent. High sensit...
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| Veröffentlicht in: | Moscow University chemistry bulletin 2018-09, Vol.73 (5), p.216-222 |
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| creator | Vokhmyanina, D. V. Karyakina, E. E. Andreev, E. A. Karyakin, A. A. |
| description | A planar multibiosensor for the simultaneous determination of glucose and lactate is developed by combining the Prussian Blue-based electrocatalyst and the protocol for immobilization of glucose oxidase and lactate oxidase enzymes from solutions with a high content of an organic solvent. High sensitivity coefficients (>80 mA M
–1
cm
–2
for lactate and >20 mA M
–1
cm
–2
for glucose) are demonstrated by the multibiosensors operating in the flow-injection mode in a thin-layer measuring cell. The linear range of the analyzed concentration is 1–500 μM for lactate and 5–1000 μM for glucose. A multibiosensor can be used repeatedly (the exhibited operational stability is not less than 100 measurements without the need for recalibration), which allows using it for the analysis of diluted blood samples and blood serum. The electrocatalytic system with a multibiosensor demonstrates performance characteristics that are superior to the commercial analyzers. |
| doi_str_mv | 10.3103/S0027131418050127 |
| format | Article |
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–1
cm
–2
for lactate and >20 mA M
–1
cm
–2
for glucose) are demonstrated by the multibiosensors operating in the flow-injection mode in a thin-layer measuring cell. The linear range of the analyzed concentration is 1–500 μM for lactate and 5–1000 μM for glucose. A multibiosensor can be used repeatedly (the exhibited operational stability is not less than 100 measurements without the need for recalibration), which allows using it for the analysis of diluted blood samples and blood serum. The electrocatalytic system with a multibiosensor demonstrates performance characteristics that are superior to the commercial analyzers.</description><identifier>ISSN: 0027-1314</identifier><identifier>EISSN: 1935-0260</identifier><identifier>DOI: 10.3103/S0027131418050127</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>Analyzers ; Blood ; Chemistry ; Chemistry and Materials Science ; Chemistry/Food Science ; Glucose ; Glucose oxidase ; Ions ; Pigments</subject><ispartof>Moscow University chemistry bulletin, 2018-09, Vol.73 (5), p.216-222</ispartof><rights>Allerton Press, Inc. 2018</rights><rights>Copyright Springer Science & Business Media 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-fbab5e5a32ce7e68397591daba708eb4c25f1ec5bd4aacdd391a8cbe67464e103</citedby><cites>FETCH-LOGICAL-c316t-fbab5e5a32ce7e68397591daba708eb4c25f1ec5bd4aacdd391a8cbe67464e103</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.3103/S0027131418050127$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.3103/S0027131418050127$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Vokhmyanina, D. V.</creatorcontrib><creatorcontrib>Karyakina, E. E.</creatorcontrib><creatorcontrib>Andreev, E. A.</creatorcontrib><creatorcontrib>Karyakin, A. A.</creatorcontrib><title>Prussian Blue-Based Thin-Layer Flow-Injection Multibiosensor for Simultaneous Determination of Glucose and Lactate</title><title>Moscow University chemistry bulletin</title><addtitle>Moscow Univ. Chem. Bull</addtitle><description>A planar multibiosensor for the simultaneous determination of glucose and lactate is developed by combining the Prussian Blue-based electrocatalyst and the protocol for immobilization of glucose oxidase and lactate oxidase enzymes from solutions with a high content of an organic solvent. High sensitivity coefficients (>80 mA M
–1
cm
–2
for lactate and >20 mA M
–1
cm
–2
for glucose) are demonstrated by the multibiosensors operating in the flow-injection mode in a thin-layer measuring cell. The linear range of the analyzed concentration is 1–500 μM for lactate and 5–1000 μM for glucose. A multibiosensor can be used repeatedly (the exhibited operational stability is not less than 100 measurements without the need for recalibration), which allows using it for the analysis of diluted blood samples and blood serum. The electrocatalytic system with a multibiosensor demonstrates performance characteristics that are superior to the commercial analyzers.</description><subject>Analyzers</subject><subject>Blood</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chemistry/Food Science</subject><subject>Glucose</subject><subject>Glucose oxidase</subject><subject>Ions</subject><subject>Pigments</subject><issn>0027-1314</issn><issn>1935-0260</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1kE1Lw0AQhhdRsFZ_gLcFz6v7kc0mR6u2FioKrecw2Uw0Jd2tuwnSf29qBQ_iYRiY92PgIeRS8GsluLpZci6NUCIRGddcSHNERiJXmnGZ8mMy2stsr5-SsxjXnOtUKjMi4SX0MTbg6KTtkU0gYkVX741jC9hhoNPWf7K5W6PtGu_oU992Tdn4iC76QOthls1mOIJD30d6jx2GTePg2-1rOmt7O7gpuIouwHbQ4Tk5qaGNePGzx-R1-rC6e2SL59n87nbBrBJpx-oSSo0alLRoMM1UbnQuKijB8AzLxEpdC7S6rBIAW1UqF5DZElOTpAkOTMbk6tC7Df6jx9gVa98HN7wspFBGc2MGBmMiDi4bfIwB62Ibmg2EXSF4sUdb_EE7ZOQhEweve8Pw2_x_6AsCAXy8</recordid><startdate>20180901</startdate><enddate>20180901</enddate><creator>Vokhmyanina, D. V.</creator><creator>Karyakina, E. E.</creator><creator>Andreev, E. A.</creator><creator>Karyakin, A. A.</creator><general>Pleiades Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20180901</creationdate><title>Prussian Blue-Based Thin-Layer Flow-Injection Multibiosensor for Simultaneous Determination of Glucose and Lactate</title><author>Vokhmyanina, D. V. ; Karyakina, E. E. ; Andreev, E. A. ; Karyakin, A. A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-fbab5e5a32ce7e68397591daba708eb4c25f1ec5bd4aacdd391a8cbe67464e103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Analyzers</topic><topic>Blood</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chemistry/Food Science</topic><topic>Glucose</topic><topic>Glucose oxidase</topic><topic>Ions</topic><topic>Pigments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vokhmyanina, D. V.</creatorcontrib><creatorcontrib>Karyakina, E. E.</creatorcontrib><creatorcontrib>Andreev, E. A.</creatorcontrib><creatorcontrib>Karyakin, A. A.</creatorcontrib><collection>CrossRef</collection><jtitle>Moscow University chemistry bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vokhmyanina, D. V.</au><au>Karyakina, E. E.</au><au>Andreev, E. A.</au><au>Karyakin, A. A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prussian Blue-Based Thin-Layer Flow-Injection Multibiosensor for Simultaneous Determination of Glucose and Lactate</atitle><jtitle>Moscow University chemistry bulletin</jtitle><stitle>Moscow Univ. Chem. Bull</stitle><date>2018-09-01</date><risdate>2018</risdate><volume>73</volume><issue>5</issue><spage>216</spage><epage>222</epage><pages>216-222</pages><issn>0027-1314</issn><eissn>1935-0260</eissn><abstract>A planar multibiosensor for the simultaneous determination of glucose and lactate is developed by combining the Prussian Blue-based electrocatalyst and the protocol for immobilization of glucose oxidase and lactate oxidase enzymes from solutions with a high content of an organic solvent. High sensitivity coefficients (>80 mA M
–1
cm
–2
for lactate and >20 mA M
–1
cm
–2
for glucose) are demonstrated by the multibiosensors operating in the flow-injection mode in a thin-layer measuring cell. The linear range of the analyzed concentration is 1–500 μM for lactate and 5–1000 μM for glucose. A multibiosensor can be used repeatedly (the exhibited operational stability is not less than 100 measurements without the need for recalibration), which allows using it for the analysis of diluted blood samples and blood serum. The electrocatalytic system with a multibiosensor demonstrates performance characteristics that are superior to the commercial analyzers.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><doi>10.3103/S0027131418050127</doi><tpages>7</tpages></addata></record> |
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| language | eng |
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| source | SpringerNature Journals |
| subjects | Analyzers Blood Chemistry Chemistry and Materials Science Chemistry/Food Science Glucose Glucose oxidase Ions Pigments |
| title | Prussian Blue-Based Thin-Layer Flow-Injection Multibiosensor for Simultaneous Determination of Glucose and Lactate |
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