Analysis of DNA fragmentation of porcine embryos exposed to cryoprotectants

The chemical toxicity of cryoprotectants to porcine embryos was examined by the evaluation of survival and DNA damage after exposure to cryoprotectants. Porcine blastocysts were exposed to 10% of ethylene glycol (EG), 1,2-propanediol (PD) or glycerol (GLY) for 1 h at room temperature (23-25 degrees...

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Veröffentlicht in:	Reproduction in domestic animals 2005-10, Vol.40 (5), p.429-432
Hauptverfasser:	Rajaei, F, Karja, N.W.K, Agung, B, Wongsrikeao, P, Taniguchi, M, Murakami, M, Sambuu, R, Nii, M, Otoi, T
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal reproduction Animals biochemical mechanisms Biological and medical sciences blastocyst Blastocyst - cytology Blastocyst - drug effects Blastocyst - physiology Cryopreservation - methods Cryopreservation - veterinary cryoprotectants Cryoprotective Agents - pharmacology Deoxyribonucleic acid DNA DNA damage DNA Damage - drug effects DNA Fragmentation - drug effects Embryo, Mammalian - cytology Embryo, Mammalian - drug effects Embryo, Mammalian - physiology embryonic mortality Embryos embryotoxicity ethylene glycol Ethylene Glycol - pharmacology Fundamental and applied biological sciences. Psychology glycerol Glycerol - pharmacology Hogs In Vitro Techniques Mammalian reproduction. General aspects propylene glycol Propylene Glycol - pharmacology Survival analysis swine Swine - embryology Swine - physiology Temperature Time Factors Toxicity Vertebrates: reproduction
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