Analysis of DNA fragmentation of porcine embryos exposed to cryoprotectants
The chemical toxicity of cryoprotectants to porcine embryos was examined by the evaluation of survival and DNA damage after exposure to cryoprotectants. Porcine blastocysts were exposed to 10% of ethylene glycol (EG), 1,2-propanediol (PD) or glycerol (GLY) for 1 h at room temperature (23-25 degrees...
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| Veröffentlicht in: | Reproduction in domestic animals 2005-10, Vol.40 (5), p.429-432 |
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| container_title | Reproduction in domestic animals |
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| creator | Rajaei, F Karja, N.W.K Agung, B Wongsrikeao, P Taniguchi, M Murakami, M Sambuu, R Nii, M Otoi, T |
| description | The chemical toxicity of cryoprotectants to porcine embryos was examined by the evaluation of survival and DNA damage after exposure to cryoprotectants. Porcine blastocysts were exposed to 10% of ethylene glycol (EG), 1,2-propanediol (PD) or glycerol (GLY) for 1 h at room temperature (23-25 degrees C) and then cultured in vitro for 24 h. The survival rates of blastocysts exposed to PD and GLY were significantly lower than those of control blastocysts in which the embryos were exposed to carrier solution without cryoprotectants. Significantly more DNA-fragmented nuclei occurred in the cryoprotectant-exposed blastocysts, compared with the control blastocysts. Moreover, the indices of DNA-fragmented nuclei in the blastocysts without blastocoele re-formation after culture were significantly higher than those with blastocoele re-formation, irrespective of the exposure treatment. These results indicate that the exposure of porcine blastocysts to cryoprotectant decreases the survival rates and increases the DNA-fragmented nuclei in embryos. |
| doi_str_mv | 10.1111/j.1439-0531.2005.00585.x |
| format | Article |
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Porcine blastocysts were exposed to 10% of ethylene glycol (EG), 1,2-propanediol (PD) or glycerol (GLY) for 1 h at room temperature (23-25 degrees C) and then cultured in vitro for 24 h. The survival rates of blastocysts exposed to PD and GLY were significantly lower than those of control blastocysts in which the embryos were exposed to carrier solution without cryoprotectants. Significantly more DNA-fragmented nuclei occurred in the cryoprotectant-exposed blastocysts, compared with the control blastocysts. Moreover, the indices of DNA-fragmented nuclei in the blastocysts without blastocoele re-formation after culture were significantly higher than those with blastocoele re-formation, irrespective of the exposure treatment. These results indicate that the exposure of porcine blastocysts to cryoprotectant decreases the survival rates and increases the DNA-fragmented nuclei in embryos.</description><identifier>ISSN: 0936-6768</identifier><identifier>EISSN: 1439-0531</identifier><identifier>DOI: 10.1111/j.1439-0531.2005.00585.x</identifier><identifier>PMID: 16149947</identifier><language>eng</language><publisher>Berlin, Germany: Blackwell Verlag GmbH</publisher><subject>Animal reproduction ; Animals ; biochemical mechanisms ; Biological and medical sciences ; blastocyst ; Blastocyst - cytology ; Blastocyst - drug effects ; Blastocyst - physiology ; Cryopreservation - methods ; Cryopreservation - veterinary ; cryoprotectants ; Cryoprotective Agents - pharmacology ; Deoxyribonucleic acid ; DNA ; DNA damage ; DNA Damage - drug effects ; DNA Fragmentation - drug effects ; Embryo, Mammalian - cytology ; Embryo, Mammalian - drug effects ; Embryo, Mammalian - physiology ; embryonic mortality ; Embryos ; embryotoxicity ; ethylene glycol ; Ethylene Glycol - pharmacology ; Fundamental and applied biological sciences. Psychology ; glycerol ; Glycerol - pharmacology ; Hogs ; In Vitro Techniques ; Mammalian reproduction. General aspects ; propylene glycol ; Propylene Glycol - pharmacology ; Survival analysis ; swine ; Swine - embryology ; Swine - physiology ; Temperature ; Time Factors ; Toxicity ; Vertebrates: reproduction</subject><ispartof>Reproduction in domestic animals, 2005-10, Vol.40 (5), p.429-432</ispartof><rights>2005 INIST-CNRS</rights><rights>2005 Blackwell Verlag, Berlin</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4855-b57ad8da15afd0c15a992a1ec0ae44575bb96ef547227a615684303275a0dc133</citedby><cites>FETCH-LOGICAL-c4855-b57ad8da15afd0c15a992a1ec0ae44575bb96ef547227a615684303275a0dc133</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1439-0531.2005.00585.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1439-0531.2005.00585.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17068166$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16149947$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rajaei, F</creatorcontrib><creatorcontrib>Karja, N.W.K</creatorcontrib><creatorcontrib>Agung, B</creatorcontrib><creatorcontrib>Wongsrikeao, P</creatorcontrib><creatorcontrib>Taniguchi, M</creatorcontrib><creatorcontrib>Murakami, M</creatorcontrib><creatorcontrib>Sambuu, R</creatorcontrib><creatorcontrib>Nii, M</creatorcontrib><creatorcontrib>Otoi, T</creatorcontrib><title>Analysis of DNA fragmentation of porcine embryos exposed to cryoprotectants</title><title>Reproduction in domestic animals</title><addtitle>Reprod Domest Anim</addtitle><description>The chemical toxicity of cryoprotectants to porcine embryos was examined by the evaluation of survival and DNA damage after exposure to cryoprotectants. Porcine blastocysts were exposed to 10% of ethylene glycol (EG), 1,2-propanediol (PD) or glycerol (GLY) for 1 h at room temperature (23-25 degrees C) and then cultured in vitro for 24 h. The survival rates of blastocysts exposed to PD and GLY were significantly lower than those of control blastocysts in which the embryos were exposed to carrier solution without cryoprotectants. Significantly more DNA-fragmented nuclei occurred in the cryoprotectant-exposed blastocysts, compared with the control blastocysts. Moreover, the indices of DNA-fragmented nuclei in the blastocysts without blastocoele re-formation after culture were significantly higher than those with blastocoele re-formation, irrespective of the exposure treatment. These results indicate that the exposure of porcine blastocysts to cryoprotectant decreases the survival rates and increases the DNA-fragmented nuclei in embryos.</description><subject>Animal reproduction</subject><subject>Animals</subject><subject>biochemical mechanisms</subject><subject>Biological and medical sciences</subject><subject>blastocyst</subject><subject>Blastocyst - cytology</subject><subject>Blastocyst - drug effects</subject><subject>Blastocyst - physiology</subject><subject>Cryopreservation - methods</subject><subject>Cryopreservation - veterinary</subject><subject>cryoprotectants</subject><subject>Cryoprotective Agents - pharmacology</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA damage</subject><subject>DNA Damage - drug effects</subject><subject>DNA Fragmentation - drug effects</subject><subject>Embryo, Mammalian - cytology</subject><subject>Embryo, Mammalian - drug effects</subject><subject>Embryo, Mammalian - physiology</subject><subject>embryonic mortality</subject><subject>Embryos</subject><subject>embryotoxicity</subject><subject>ethylene glycol</subject><subject>Ethylene Glycol - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>glycerol</subject><subject>Glycerol - pharmacology</subject><subject>Hogs</subject><subject>In Vitro Techniques</subject><subject>Mammalian reproduction. General aspects</subject><subject>propylene glycol</subject><subject>Propylene Glycol - pharmacology</subject><subject>Survival analysis</subject><subject>swine</subject><subject>Swine - embryology</subject><subject>Swine - physiology</subject><subject>Temperature</subject><subject>Time Factors</subject><subject>Toxicity</subject><subject>Vertebrates: reproduction</subject><issn>0936-6768</issn><issn>1439-0531</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkF1v0zAUhi0EYmXwFyBC4jLhOP6WuKk2KGjTkGDTLq0Tx5lS2rjYqWj_PQ6ptlssWceyn9c-fggpKFQ0j4_rinJmShCMVjWAqPLUojo8I4vHg-dkAYbJUiqpz8irlNYAVGilXpIzKik3hqsFuVoOuDmmPhWhKy5vlkUX8WHrhxHHPgzT5i5E1w--8NsmHkMq_GEXkm-LMRQub-xiGL0bcRjTa_Kiw03yb071nNx9-Xx78bW8_r76drG8Lh3XQpSNUNjqFqnArgWXizE1Uu8APedCiaYx0neCq7pWKKmQmjNgtRIIraOMnZP387357d97n0a7DvuY_5FsTZnimqoJ0jPkYkgp-s7uYr_FeLQU7CTRru3kyk6u7CTR_pNoDzn69nT_vtn69il4spaBDycAk8NNVja4Pj1xCqSmUmbu08z96Tf--N8N2B-Xy7zI8XKO92n0h8c4xl9WKqaEvb9ZWViZ-yvDVvY28-9mvsNg8SHmlu5-1kAZUJA1V4L9BRU-pEE</recordid><startdate>200510</startdate><enddate>200510</enddate><creator>Rajaei, F</creator><creator>Karja, N.W.K</creator><creator>Agung, B</creator><creator>Wongsrikeao, P</creator><creator>Taniguchi, M</creator><creator>Murakami, M</creator><creator>Sambuu, R</creator><creator>Nii, M</creator><creator>Otoi, T</creator><general>Blackwell Verlag GmbH</general><general>Blackwell Science</general><general>Blackwell Publishing Ltd</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>200510</creationdate><title>Analysis of DNA fragmentation of porcine embryos exposed to cryoprotectants</title><author>Rajaei, F ; Karja, N.W.K ; Agung, B ; Wongsrikeao, P ; Taniguchi, M ; Murakami, M ; Sambuu, R ; Nii, M ; Otoi, T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4855-b57ad8da15afd0c15a992a1ec0ae44575bb96ef547227a615684303275a0dc133</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animal reproduction</topic><topic>Animals</topic><topic>biochemical mechanisms</topic><topic>Biological and medical sciences</topic><topic>blastocyst</topic><topic>Blastocyst - cytology</topic><topic>Blastocyst - drug effects</topic><topic>Blastocyst - physiology</topic><topic>Cryopreservation - methods</topic><topic>Cryopreservation - veterinary</topic><topic>cryoprotectants</topic><topic>Cryoprotective Agents - pharmacology</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA damage</topic><topic>DNA Damage - drug effects</topic><topic>DNA Fragmentation - drug effects</topic><topic>Embryo, Mammalian - cytology</topic><topic>Embryo, Mammalian - drug effects</topic><topic>Embryo, Mammalian - physiology</topic><topic>embryonic mortality</topic><topic>Embryos</topic><topic>embryotoxicity</topic><topic>ethylene glycol</topic><topic>Ethylene Glycol - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>glycerol</topic><topic>Glycerol - pharmacology</topic><topic>Hogs</topic><topic>In Vitro Techniques</topic><topic>Mammalian reproduction. General aspects</topic><topic>propylene glycol</topic><topic>Propylene Glycol - pharmacology</topic><topic>Survival analysis</topic><topic>swine</topic><topic>Swine - embryology</topic><topic>Swine - physiology</topic><topic>Temperature</topic><topic>Time Factors</topic><topic>Toxicity</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rajaei, F</creatorcontrib><creatorcontrib>Karja, N.W.K</creatorcontrib><creatorcontrib>Agung, B</creatorcontrib><creatorcontrib>Wongsrikeao, P</creatorcontrib><creatorcontrib>Taniguchi, M</creatorcontrib><creatorcontrib>Murakami, M</creatorcontrib><creatorcontrib>Sambuu, R</creatorcontrib><creatorcontrib>Nii, M</creatorcontrib><creatorcontrib>Otoi, T</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Reproduction in domestic animals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rajaei, F</au><au>Karja, N.W.K</au><au>Agung, B</au><au>Wongsrikeao, P</au><au>Taniguchi, M</au><au>Murakami, M</au><au>Sambuu, R</au><au>Nii, M</au><au>Otoi, T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of DNA fragmentation of porcine embryos exposed to cryoprotectants</atitle><jtitle>Reproduction in domestic animals</jtitle><addtitle>Reprod Domest Anim</addtitle><date>2005-10</date><risdate>2005</risdate><volume>40</volume><issue>5</issue><spage>429</spage><epage>432</epage><pages>429-432</pages><issn>0936-6768</issn><eissn>1439-0531</eissn><abstract>The chemical toxicity of cryoprotectants to porcine embryos was examined by the evaluation of survival and DNA damage after exposure to cryoprotectants. Porcine blastocysts were exposed to 10% of ethylene glycol (EG), 1,2-propanediol (PD) or glycerol (GLY) for 1 h at room temperature (23-25 degrees C) and then cultured in vitro for 24 h. The survival rates of blastocysts exposed to PD and GLY were significantly lower than those of control blastocysts in which the embryos were exposed to carrier solution without cryoprotectants. Significantly more DNA-fragmented nuclei occurred in the cryoprotectant-exposed blastocysts, compared with the control blastocysts. Moreover, the indices of DNA-fragmented nuclei in the blastocysts without blastocoele re-formation after culture were significantly higher than those with blastocoele re-formation, irrespective of the exposure treatment. These results indicate that the exposure of porcine blastocysts to cryoprotectant decreases the survival rates and increases the DNA-fragmented nuclei in embryos.</abstract><cop>Berlin, Germany</cop><pub>Blackwell Verlag GmbH</pub><pmid>16149947</pmid><doi>10.1111/j.1439-0531.2005.00585.x</doi><tpages>4</tpages></addata></record> |
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| subjects | Animal reproduction Animals biochemical mechanisms Biological and medical sciences blastocyst Blastocyst - cytology Blastocyst - drug effects Blastocyst - physiology Cryopreservation - methods Cryopreservation - veterinary cryoprotectants Cryoprotective Agents - pharmacology Deoxyribonucleic acid DNA DNA damage DNA Damage - drug effects DNA Fragmentation - drug effects Embryo, Mammalian - cytology Embryo, Mammalian - drug effects Embryo, Mammalian - physiology embryonic mortality Embryos embryotoxicity ethylene glycol Ethylene Glycol - pharmacology Fundamental and applied biological sciences. Psychology glycerol Glycerol - pharmacology Hogs In Vitro Techniques Mammalian reproduction. General aspects propylene glycol Propylene Glycol - pharmacology Survival analysis swine Swine - embryology Swine - physiology Temperature Time Factors Toxicity Vertebrates: reproduction |
| title | Analysis of DNA fragmentation of porcine embryos exposed to cryoprotectants |
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