Structure of a Synaptic [gamma][delta] Resolvase Tetramer Covalently Linked to Two Cleaved DNAs
The structure of a synaptic intermediate of the site-specific recombinase [gamma][delta] resolvase covalently linked through Ser¹⁰ to two cleaved duplex DNAs has been determined at 3.4 angstrom resolution. This resolvase, activated for recombination by mutations, forms a tetramer whose structure is...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 2005-08, Vol.309 (5738), p.1210-1215 |
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| container_title | Science (American Association for the Advancement of Science) |
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| creator | Li, Weikai Kamtekar, Satwik Xiong, Yong Sarkis, Gary J Grindley, Nigel D. F Steitz, Thomas A |
| description | The structure of a synaptic intermediate of the site-specific recombinase [gamma][delta] resolvase covalently linked through Ser¹⁰ to two cleaved duplex DNAs has been determined at 3.4 angstrom resolution. This resolvase, activated for recombination by mutations, forms a tetramer whose structure is substantially changed from that of a presynaptic complex between dimeric resolvase and the cleavage site DNA. Because the two cleaved DNA duplexes that are to be recombined lie on opposite sides of the core tetramer, large movements of both protein and DNA are required to achieve strand exchange. The two dimers linked to the DNAs that are to be recombined are held together by a flat interface. This may allow a 180° rotation of one dimer relative to the other in order to reposition the DNA duplexes for strand exchange. |
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F ; Steitz, Thomas A</creator><creatorcontrib>Li, Weikai ; Kamtekar, Satwik ; Xiong, Yong ; Sarkis, Gary J ; Grindley, Nigel D. F ; Steitz, Thomas A</creatorcontrib><description>The structure of a synaptic intermediate of the site-specific recombinase [gamma][delta] resolvase covalently linked through Ser¹⁰ to two cleaved duplex DNAs has been determined at 3.4 angstrom resolution. This resolvase, activated for recombination by mutations, forms a tetramer whose structure is substantially changed from that of a presynaptic complex between dimeric resolvase and the cleavage site DNA. Because the two cleaved DNA duplexes that are to be recombined lie on opposite sides of the core tetramer, large movements of both protein and DNA are required to achieve strand exchange. The two dimers linked to the DNAs that are to be recombined are held together by a flat interface. 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This may allow a 180° rotation of one dimer relative to the other in order to reposition the DNA duplexes for strand exchange.</description><subject>Action potential</subject><subject>Action potentials (Electrophysiology)</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Genetics</subject><subject>Mutation</subject><subject>Neural transmission</subject><subject>Synaptic transmission</subject><subject>Topology</subject><issn>0036-8075</issn><issn>1095-9203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNotjl9LwzAUxYsoOKefweB74XZp0uZx1L8wHLj6NEa4bW7LZtfMJp3u2xuYT4fD-XHOuYgmCSgRqxnwy2gCwGWcQyauoxvndgAhU3wS6ZUfxtqPAzHbMGSrU48Hv63ZusX9HjdrQ53HDfsgZ7sjOmIl-QH3NLDCHrGj3ncnttj2X2SYt6z8sazoCI_BPr7P3W101WDn6O5fp1H5_FQWr_Fi-fJWzBdxkysVN9iAQANGCiMp41CRQZ7lkiitTC1lVhuoUpELrKqUalCkJMdKGpFWnBo-jR7OtYfBfo_kvN7ZcejDop4lXEKSSRWg-Ay14bfe9rXtPf362nYdtaTDn2Kp5wkXeZqonAf-_sw3aDW2w9bpz9UMEg4JhNY843-FNmnr</recordid><startdate>20050819</startdate><enddate>20050819</enddate><creator>Li, Weikai</creator><creator>Kamtekar, Satwik</creator><creator>Xiong, Yong</creator><creator>Sarkis, Gary J</creator><creator>Grindley, Nigel D. 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F</au><au>Steitz, Thomas A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structure of a Synaptic [gamma][delta] Resolvase Tetramer Covalently Linked to Two Cleaved DNAs</atitle><jtitle>Science (American Association for the Advancement of Science)</jtitle><addtitle>Science</addtitle><date>2005-08-19</date><risdate>2005</risdate><volume>309</volume><issue>5738</issue><spage>1210</spage><epage>1215</epage><pages>1210-1215</pages><issn>0036-8075</issn><eissn>1095-9203</eissn><coden>SCIEAS</coden><abstract>The structure of a synaptic intermediate of the site-specific recombinase [gamma][delta] resolvase covalently linked through Ser¹⁰ to two cleaved duplex DNAs has been determined at 3.4 angstrom resolution. This resolvase, activated for recombination by mutations, forms a tetramer whose structure is substantially changed from that of a presynaptic complex between dimeric resolvase and the cleavage site DNA. Because the two cleaved DNA duplexes that are to be recombined lie on opposite sides of the core tetramer, large movements of both protein and DNA are required to achieve strand exchange. The two dimers linked to the DNAs that are to be recombined are held together by a flat interface. This may allow a 180° rotation of one dimer relative to the other in order to reposition the DNA duplexes for strand exchange.</abstract><cop>Washington</cop><pub>American Association for the Advancement of Science</pub><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 2005-08, Vol.309 (5738), p.1210-1215 |
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| source | American Association for the Advancement of Science; Jstor Complete Legacy |
| subjects | Action potential Action potentials (Electrophysiology) Deoxyribonucleic acid DNA Genetics Mutation Neural transmission Synaptic transmission Topology |
| title | Structure of a Synaptic [gamma][delta] Resolvase Tetramer Covalently Linked to Two Cleaved DNAs |
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