Gastrointestinal dysmotility in 5-fluorouracil-induced intestinal mucositis outlasts inflammatory process resolution
Aim To evaluate gastrointestinal motility during 5-fluorouracil (5-FU)-induced intestinal mucositis. Materials and methods Wistar rats received 5-FU (150 mg kg −1 , i.p.) or saline. After the 1st, 3rd, 5th, 15th and 30th day, sections of duodenum, jejunum and ileum were removed for assessment of epi...
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| Veröffentlicht in: | Cancer chemotherapy and pharmacology 2008-12, Vol.63 (1), p.91-98 |
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| creator | Soares, Pedro M. G. Mota, José Maurício S. C. Gomes, Antoniella S. Oliveira, Ricardo B. Assreuy, Ana Maria S. Brito, Gerly Anne C. Santos, Armênio A. Ribeiro, Ronaldo A. Souza, Marcellus H. L. P. |
| description | Aim
To evaluate gastrointestinal motility during 5-fluorouracil (5-FU)-induced intestinal mucositis.
Materials and methods
Wistar rats received 5-FU (150 mg kg
−1
, i.p.) or saline. After the 1st, 3rd, 5th, 15th and 30th day, sections of duodenum, jejunum and ileum were removed for assessment of epithelial damage, apoptotic and mitotic indexes, MPO activity and GSH concentration. In order to study gastrointestinal motility, on the 3rd or 15th day after 5-FU treatment, gastric emptying in vivo was measured by scintilographic method, and stomach or duodenal smooth muscle contractions induced by CCh were evaluated in vitro.
Results
On the third day of treatment, 5-FU induced a significant villi shortening, an increase in crypt depth and intestinal MPO activity and a decrease in villus/crypt ratio and GSH concentration. On the first day after 5-FU there was an increase in the apoptosis index and a decrease in the mitosis index in all intestinal segments. After the 15th day of 5-FU treatment, a complete reversion of all these parameters was observed. There was a delay in gastric emptying in vivo and a significant increase in gastric fundus and duodenum smooth muscle contraction, after both the 3rd and 15th day.
Conclusion
5-FU-induced gastrointestinal dysmotility outlasts intestinal mucositis. |
| doi_str_mv | 10.1007/s00280-008-0715-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_213504885</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1565723881</sourcerecordid><originalsourceid>FETCH-LOGICAL-c465t-5221206d44624ea76ea574a1007af0ac560d66d0f870d7bd5082438e2e179c3c3</originalsourceid><addsrcrecordid>eNp1kE9rGzEQxUVoqJ2kH6CXshR6VDP6tysfi0mTQqCX5ixkSRtktKtUoz3421fGpsklpznM77158wj5zOA7AxhuEYBroACawsAU3VyQNZOCU9BSfCBrEFJSNYBckSvEPQBIJsRHsmJacClBrkm9t1hLjnMNWONsU-cPOOUaU6yHLs6domNacslLsS4mGme_uOC7N4JpcRljjdjlpaZmh207JjtNtuZy6F5KdgGxKwFzWmrM8w25HG3C8Ok8r8nTz7s_2wf6-Pv-1_bHI3WyV5UqzhmH3kvZcxns0AerBmmPr9sRrFM9-L73MOoB_LDzCjSXQgce2LBxwolr8vXk2yL8XVpes29_tMxoOBMKpNaqQewEuZIRSxjNS4mTLQfDwBxvmVPNptVsjjWbTdN8ORsvuyn4V8W51wZ8OwMWnU1jsbOL-J_jDNim-TWOnzhsq_k5lNeE71__B63Pl2M</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>213504885</pqid></control><display><type>article</type><title>Gastrointestinal dysmotility in 5-fluorouracil-induced intestinal mucositis outlasts inflammatory process resolution</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Soares, Pedro M. G. ; Mota, José Maurício S. C. ; Gomes, Antoniella S. ; Oliveira, Ricardo B. ; Assreuy, Ana Maria S. ; Brito, Gerly Anne C. ; Santos, Armênio A. ; Ribeiro, Ronaldo A. ; Souza, Marcellus H. L. P.</creator><creatorcontrib>Soares, Pedro M. G. ; Mota, José Maurício S. C. ; Gomes, Antoniella S. ; Oliveira, Ricardo B. ; Assreuy, Ana Maria S. ; Brito, Gerly Anne C. ; Santos, Armênio A. ; Ribeiro, Ronaldo A. ; Souza, Marcellus H. L. P.</creatorcontrib><description>Aim
To evaluate gastrointestinal motility during 5-fluorouracil (5-FU)-induced intestinal mucositis.
Materials and methods
Wistar rats received 5-FU (150 mg kg
−1
, i.p.) or saline. After the 1st, 3rd, 5th, 15th and 30th day, sections of duodenum, jejunum and ileum were removed for assessment of epithelial damage, apoptotic and mitotic indexes, MPO activity and GSH concentration. In order to study gastrointestinal motility, on the 3rd or 15th day after 5-FU treatment, gastric emptying in vivo was measured by scintilographic method, and stomach or duodenal smooth muscle contractions induced by CCh were evaluated in vitro.
Results
On the third day of treatment, 5-FU induced a significant villi shortening, an increase in crypt depth and intestinal MPO activity and a decrease in villus/crypt ratio and GSH concentration. On the first day after 5-FU there was an increase in the apoptosis index and a decrease in the mitosis index in all intestinal segments. After the 15th day of 5-FU treatment, a complete reversion of all these parameters was observed. There was a delay in gastric emptying in vivo and a significant increase in gastric fundus and duodenum smooth muscle contraction, after both the 3rd and 15th day.
Conclusion
5-FU-induced gastrointestinal dysmotility outlasts intestinal mucositis.</description><identifier>ISSN: 0344-5704</identifier><identifier>EISSN: 1432-0843</identifier><identifier>DOI: 10.1007/s00280-008-0715-9</identifier><identifier>PMID: 18324404</identifier><identifier>CODEN: CCPHDZ</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Animals ; Antimetabolites, Antineoplastic - toxicity ; Antineoplastic agents ; Apoptosis - drug effects ; Biological and medical sciences ; Cancer Research ; Carbachol - pharmacology ; Fluorouracil - toxicity ; Gastric Emptying - drug effects ; Gastric Fundus - pathology ; Gastrointestinal Motility - drug effects ; Glutathione - analysis ; Intestinal Diseases - chemically induced ; Intestinal Diseases - physiopathology ; Intestine, Small - pathology ; Male ; Medical sciences ; Medicine ; Medicine & Public Health ; Mitotic Index ; Mucositis - chemically induced ; Mucositis - physiopathology ; Oncology ; Original Article ; Peroxidase - analysis ; Pharmacology. Drug treatments ; Pharmacology/Toxicology ; Random Allocation ; Rats ; Rats, Wistar ; Time Factors</subject><ispartof>Cancer chemotherapy and pharmacology, 2008-12, Vol.63 (1), p.91-98</ispartof><rights>Springer-Verlag 2008</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c465t-5221206d44624ea76ea574a1007af0ac560d66d0f870d7bd5082438e2e179c3c3</citedby><cites>FETCH-LOGICAL-c465t-5221206d44624ea76ea574a1007af0ac560d66d0f870d7bd5082438e2e179c3c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00280-008-0715-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00280-008-0715-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21019008$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18324404$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Soares, Pedro M. G.</creatorcontrib><creatorcontrib>Mota, José Maurício S. C.</creatorcontrib><creatorcontrib>Gomes, Antoniella S.</creatorcontrib><creatorcontrib>Oliveira, Ricardo B.</creatorcontrib><creatorcontrib>Assreuy, Ana Maria S.</creatorcontrib><creatorcontrib>Brito, Gerly Anne C.</creatorcontrib><creatorcontrib>Santos, Armênio A.</creatorcontrib><creatorcontrib>Ribeiro, Ronaldo A.</creatorcontrib><creatorcontrib>Souza, Marcellus H. L. P.</creatorcontrib><title>Gastrointestinal dysmotility in 5-fluorouracil-induced intestinal mucositis outlasts inflammatory process resolution</title><title>Cancer chemotherapy and pharmacology</title><addtitle>Cancer Chemother Pharmacol</addtitle><addtitle>Cancer Chemother Pharmacol</addtitle><description>Aim
To evaluate gastrointestinal motility during 5-fluorouracil (5-FU)-induced intestinal mucositis.
Materials and methods
Wistar rats received 5-FU (150 mg kg
−1
, i.p.) or saline. After the 1st, 3rd, 5th, 15th and 30th day, sections of duodenum, jejunum and ileum were removed for assessment of epithelial damage, apoptotic and mitotic indexes, MPO activity and GSH concentration. In order to study gastrointestinal motility, on the 3rd or 15th day after 5-FU treatment, gastric emptying in vivo was measured by scintilographic method, and stomach or duodenal smooth muscle contractions induced by CCh were evaluated in vitro.
Results
On the third day of treatment, 5-FU induced a significant villi shortening, an increase in crypt depth and intestinal MPO activity and a decrease in villus/crypt ratio and GSH concentration. On the first day after 5-FU there was an increase in the apoptosis index and a decrease in the mitosis index in all intestinal segments. After the 15th day of 5-FU treatment, a complete reversion of all these parameters was observed. There was a delay in gastric emptying in vivo and a significant increase in gastric fundus and duodenum smooth muscle contraction, after both the 3rd and 15th day.
Conclusion
5-FU-induced gastrointestinal dysmotility outlasts intestinal mucositis.</description><subject>Animals</subject><subject>Antimetabolites, Antineoplastic - toxicity</subject><subject>Antineoplastic agents</subject><subject>Apoptosis - drug effects</subject><subject>Biological and medical sciences</subject><subject>Cancer Research</subject><subject>Carbachol - pharmacology</subject><subject>Fluorouracil - toxicity</subject><subject>Gastric Emptying - drug effects</subject><subject>Gastric Fundus - pathology</subject><subject>Gastrointestinal Motility - drug effects</subject><subject>Glutathione - analysis</subject><subject>Intestinal Diseases - chemically induced</subject><subject>Intestinal Diseases - physiopathology</subject><subject>Intestine, Small - pathology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Mitotic Index</subject><subject>Mucositis - chemically induced</subject><subject>Mucositis - physiopathology</subject><subject>Oncology</subject><subject>Original Article</subject><subject>Peroxidase - analysis</subject><subject>Pharmacology. Drug treatments</subject><subject>Pharmacology/Toxicology</subject><subject>Random Allocation</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Time Factors</subject><issn>0344-5704</issn><issn>1432-0843</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kE9rGzEQxUVoqJ2kH6CXshR6VDP6tysfi0mTQqCX5ixkSRtktKtUoz3421fGpsklpznM77158wj5zOA7AxhuEYBroACawsAU3VyQNZOCU9BSfCBrEFJSNYBckSvEPQBIJsRHsmJacClBrkm9t1hLjnMNWONsU-cPOOUaU6yHLs6domNacslLsS4mGme_uOC7N4JpcRljjdjlpaZmh207JjtNtuZy6F5KdgGxKwFzWmrM8w25HG3C8Ok8r8nTz7s_2wf6-Pv-1_bHI3WyV5UqzhmH3kvZcxns0AerBmmPr9sRrFM9-L73MOoB_LDzCjSXQgce2LBxwolr8vXk2yL8XVpes29_tMxoOBMKpNaqQewEuZIRSxjNS4mTLQfDwBxvmVPNptVsjjWbTdN8ORsvuyn4V8W51wZ8OwMWnU1jsbOL-J_jDNim-TWOnzhsq_k5lNeE71__B63Pl2M</recordid><startdate>20081201</startdate><enddate>20081201</enddate><creator>Soares, Pedro M. G.</creator><creator>Mota, José Maurício S. C.</creator><creator>Gomes, Antoniella S.</creator><creator>Oliveira, Ricardo B.</creator><creator>Assreuy, Ana Maria S.</creator><creator>Brito, Gerly Anne C.</creator><creator>Santos, Armênio A.</creator><creator>Ribeiro, Ronaldo A.</creator><creator>Souza, Marcellus H. L. P.</creator><general>Springer-Verlag</general><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20081201</creationdate><title>Gastrointestinal dysmotility in 5-fluorouracil-induced intestinal mucositis outlasts inflammatory process resolution</title><author>Soares, Pedro M. G. ; Mota, José Maurício S. C. ; Gomes, Antoniella S. ; Oliveira, Ricardo B. ; Assreuy, Ana Maria S. ; Brito, Gerly Anne C. ; Santos, Armênio A. ; Ribeiro, Ronaldo A. ; Souza, Marcellus H. L. P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c465t-5221206d44624ea76ea574a1007af0ac560d66d0f870d7bd5082438e2e179c3c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Antimetabolites, Antineoplastic - toxicity</topic><topic>Antineoplastic agents</topic><topic>Apoptosis - drug effects</topic><topic>Biological and medical sciences</topic><topic>Cancer Research</topic><topic>Carbachol - pharmacology</topic><topic>Fluorouracil - toxicity</topic><topic>Gastric Emptying - drug effects</topic><topic>Gastric Fundus - pathology</topic><topic>Gastrointestinal Motility - drug effects</topic><topic>Glutathione - analysis</topic><topic>Intestinal Diseases - chemically induced</topic><topic>Intestinal Diseases - physiopathology</topic><topic>Intestine, Small - pathology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Mitotic Index</topic><topic>Mucositis - chemically induced</topic><topic>Mucositis - physiopathology</topic><topic>Oncology</topic><topic>Original Article</topic><topic>Peroxidase - analysis</topic><topic>Pharmacology. Drug treatments</topic><topic>Pharmacology/Toxicology</topic><topic>Random Allocation</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Soares, Pedro M. G.</creatorcontrib><creatorcontrib>Mota, José Maurício S. C.</creatorcontrib><creatorcontrib>Gomes, Antoniella S.</creatorcontrib><creatorcontrib>Oliveira, Ricardo B.</creatorcontrib><creatorcontrib>Assreuy, Ana Maria S.</creatorcontrib><creatorcontrib>Brito, Gerly Anne C.</creatorcontrib><creatorcontrib>Santos, Armênio A.</creatorcontrib><creatorcontrib>Ribeiro, Ronaldo A.</creatorcontrib><creatorcontrib>Souza, Marcellus H. L. P.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Cancer chemotherapy and pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Soares, Pedro M. G.</au><au>Mota, José Maurício S. C.</au><au>Gomes, Antoniella S.</au><au>Oliveira, Ricardo B.</au><au>Assreuy, Ana Maria S.</au><au>Brito, Gerly Anne C.</au><au>Santos, Armênio A.</au><au>Ribeiro, Ronaldo A.</au><au>Souza, Marcellus H. L. P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gastrointestinal dysmotility in 5-fluorouracil-induced intestinal mucositis outlasts inflammatory process resolution</atitle><jtitle>Cancer chemotherapy and pharmacology</jtitle><stitle>Cancer Chemother Pharmacol</stitle><addtitle>Cancer Chemother Pharmacol</addtitle><date>2008-12-01</date><risdate>2008</risdate><volume>63</volume><issue>1</issue><spage>91</spage><epage>98</epage><pages>91-98</pages><issn>0344-5704</issn><eissn>1432-0843</eissn><coden>CCPHDZ</coden><abstract>Aim
To evaluate gastrointestinal motility during 5-fluorouracil (5-FU)-induced intestinal mucositis.
Materials and methods
Wistar rats received 5-FU (150 mg kg
−1
, i.p.) or saline. After the 1st, 3rd, 5th, 15th and 30th day, sections of duodenum, jejunum and ileum were removed for assessment of epithelial damage, apoptotic and mitotic indexes, MPO activity and GSH concentration. In order to study gastrointestinal motility, on the 3rd or 15th day after 5-FU treatment, gastric emptying in vivo was measured by scintilographic method, and stomach or duodenal smooth muscle contractions induced by CCh were evaluated in vitro.
Results
On the third day of treatment, 5-FU induced a significant villi shortening, an increase in crypt depth and intestinal MPO activity and a decrease in villus/crypt ratio and GSH concentration. On the first day after 5-FU there was an increase in the apoptosis index and a decrease in the mitosis index in all intestinal segments. After the 15th day of 5-FU treatment, a complete reversion of all these parameters was observed. There was a delay in gastric emptying in vivo and a significant increase in gastric fundus and duodenum smooth muscle contraction, after both the 3rd and 15th day.
Conclusion
5-FU-induced gastrointestinal dysmotility outlasts intestinal mucositis.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>18324404</pmid><doi>10.1007/s00280-008-0715-9</doi><tpages>8</tpages></addata></record> |
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| issn | 0344-5704 1432-0843 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Animals Antimetabolites, Antineoplastic - toxicity Antineoplastic agents Apoptosis - drug effects Biological and medical sciences Cancer Research Carbachol - pharmacology Fluorouracil - toxicity Gastric Emptying - drug effects Gastric Fundus - pathology Gastrointestinal Motility - drug effects Glutathione - analysis Intestinal Diseases - chemically induced Intestinal Diseases - physiopathology Intestine, Small - pathology Male Medical sciences Medicine Medicine & Public Health Mitotic Index Mucositis - chemically induced Mucositis - physiopathology Oncology Original Article Peroxidase - analysis Pharmacology. Drug treatments Pharmacology/Toxicology Random Allocation Rats Rats, Wistar Time Factors |
| title | Gastrointestinal dysmotility in 5-fluorouracil-induced intestinal mucositis outlasts inflammatory process resolution |
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