high-throughput screen of cell-death-inducing factors in Nicotiana benthamiana identifies a novel MAPKK that mediates INF1-induced cell death signaling and non-host resistance to Pseudomonas cichorii
A high-throughput overexpression screen of Nicotiana benthamiana cDNAs identified a gene for a mitogen-activated protein kinase kinase (MAPKK) as a potent inducer of the hypersensitive response (HR)-like cell death. NbMKK1 protein is localized to the nucleus, and the N-terminal putative MAPK docking...
Gespeichert in:
| Veröffentlicht in: | The Plant journal : for cell and molecular biology 2007-03, Vol.49 (6), p.1030-1040 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1040 |
|---|---|
| container_issue | 6 |
| container_start_page | 1030 |
| container_title | The Plant journal : for cell and molecular biology |
| container_volume | 49 |
| creator | Takahashi, Yoshihiro Nasir, Khairun Hisam Bin Ito, Akiko Kanzaki, Hiroyuki Matsumura, Hideo Saitoh, Hiromasa Fujisawa, Shizuko Kamoun, Sophien Terauchi, Ryohei |
| description | A high-throughput overexpression screen of Nicotiana benthamiana cDNAs identified a gene for a mitogen-activated protein kinase kinase (MAPKK) as a potent inducer of the hypersensitive response (HR)-like cell death. NbMKK1 protein is localized to the nucleus, and the N-terminal putative MAPK docking site of NbMKK1 is required for its function as a cell-death inducer. NbMKK1-mediated leaf-cell death was compromised in leaves where NbSIPK expression was silenced by virus-induced gene silencing. A yeast two-hybrid assay showed that NbMKK1 and NbSIPK physically interact, suggesting that NbSIPK is one of the downstream targets of NbMKK1. Phytophthora infestans INF1 elicitor-mediated HR was delayed in NbMKK1-silenced plants, indicating that NbMKK1 is involved in this HR pathway. Furthermore, the resistance of N. benthamiana to a non-host pathogen Pseudomonas cichorii was compromised in NbMKK1-silenced plants. These results demonstrate that MAPK cascades involving NbMKK1 control non-host resistance including HR cell death. |
| doi_str_mv | 10.1111/j.1365-313X.2006.03022.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_213490904</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1227397011</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4482-2aa8fe4d0a749240e6e66bc3ff238af6d08c5fae82e2da1b5baad0217e651b63</originalsourceid><addsrcrecordid>eNqNks9uEzEQxlcIREvhFcBC4rjBf3adzYFDVVEoLSUSQeJmzXrHWUcbO7W90D4hr4U3iegVX8bW_L6Z0XwuCsLojOXzfjNjQtalYOLnjFMqZ1RQzmf3T4rTf4mnxSldSFrOK8ZPihcxbihlcyGr58VJjmzRVPK0-NPbdV-mPvhx3e_GRKIOiI54QzQOQ9khpL60rhu1dWtiQCcfIrGO3FrtkwUHpEWXetju77bLD2ssRgLE-V84kK_ny-trkolEtthZSDl3dXvJDlWx2zci-0Yk2rWDYeoErst6V_Y-JhIw2pjAaSTJk2XEsfNb7yASbXXvg7Uvi2cGhoivjvGsWF1-XF18Lm--fbq6OL8pdVU1vOQAjcGqozCvFryiKFHKVgtjuGjAyI42ujaADUfeAWvrFqCjnM1R1qyV4qx4eyi7C_5uxJjUxo8hjxwVZ6Ja0AWtMtQcIB18jAGN2gW7hfCgGFWTf2qjJpvUZJOa_FN7_9R9lr4-1h_bvKxH4dGwDLw7AhA1DCbkpdj4yDV1I2pWZ-7DgfttB3z47wHUavllumX9m4PegFewDrnHj--cMkHpvObTR_oLENrEEw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>213490904</pqid></control><display><type>article</type><title>high-throughput screen of cell-death-inducing factors in Nicotiana benthamiana identifies a novel MAPKK that mediates INF1-induced cell death signaling and non-host resistance to Pseudomonas cichorii</title><source>Wiley Free Content</source><source>MEDLINE</source><source>IngentaConnect Free/Open Access Journals</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Takahashi, Yoshihiro ; Nasir, Khairun Hisam Bin ; Ito, Akiko ; Kanzaki, Hiroyuki ; Matsumura, Hideo ; Saitoh, Hiromasa ; Fujisawa, Shizuko ; Kamoun, Sophien ; Terauchi, Ryohei</creator><creatorcontrib>Takahashi, Yoshihiro ; Nasir, Khairun Hisam Bin ; Ito, Akiko ; Kanzaki, Hiroyuki ; Matsumura, Hideo ; Saitoh, Hiromasa ; Fujisawa, Shizuko ; Kamoun, Sophien ; Terauchi, Ryohei</creatorcontrib><description>A high-throughput overexpression screen of Nicotiana benthamiana cDNAs identified a gene for a mitogen-activated protein kinase kinase (MAPKK) as a potent inducer of the hypersensitive response (HR)-like cell death. NbMKK1 protein is localized to the nucleus, and the N-terminal putative MAPK docking site of NbMKK1 is required for its function as a cell-death inducer. NbMKK1-mediated leaf-cell death was compromised in leaves where NbSIPK expression was silenced by virus-induced gene silencing. A yeast two-hybrid assay showed that NbMKK1 and NbSIPK physically interact, suggesting that NbSIPK is one of the downstream targets of NbMKK1. Phytophthora infestans INF1 elicitor-mediated HR was delayed in NbMKK1-silenced plants, indicating that NbMKK1 is involved in this HR pathway. Furthermore, the resistance of N. benthamiana to a non-host pathogen Pseudomonas cichorii was compromised in NbMKK1-silenced plants. These results demonstrate that MAPK cascades involving NbMKK1 control non-host resistance including HR cell death.</description><identifier>ISSN: 0960-7412</identifier><identifier>EISSN: 1365-313X</identifier><identifier>DOI: 10.1111/j.1365-313X.2006.03022.x</identifier><identifier>PMID: 17319846</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Agronomy. Soil science and plant productions ; Apoptosis ; Biological and medical sciences ; Botany ; cell death ; Cell Death - physiology ; Cell Nucleus - metabolism ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Genetics and breeding of economic plants ; Hydrogen Peroxide - metabolism ; Immunity, Innate - physiology ; Kinases ; MAP Kinase Signaling System - physiology ; mitogen-activated protein kinase kinase ; Mitogen-Activated Protein Kinase Kinases - physiology ; Nicotiana ; non-host resistance ; nuclear localization ; Pest resistance ; Phytophthora infestans ; Plant Diseases ; Plant pathogens ; Plant resistance ; Proteins ; Pseudomonas - physiology ; Signal transduction ; Signal Transduction - physiology ; Varietal selection. Specialized plant breeding, plant breeding aims ; virus-induced gene silencing ; Yeast</subject><ispartof>The Plant journal : for cell and molecular biology, 2007-03, Vol.49 (6), p.1030-1040</ispartof><rights>2007 INIST-CNRS</rights><rights>2007 The Authors Journal compilation 2007 Blackwell Publishing Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4482-2aa8fe4d0a749240e6e66bc3ff238af6d08c5fae82e2da1b5baad0217e651b63</citedby><cites>FETCH-LOGICAL-c4482-2aa8fe4d0a749240e6e66bc3ff238af6d08c5fae82e2da1b5baad0217e651b63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-313X.2006.03022.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-313X.2006.03022.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18583515$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17319846$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Takahashi, Yoshihiro</creatorcontrib><creatorcontrib>Nasir, Khairun Hisam Bin</creatorcontrib><creatorcontrib>Ito, Akiko</creatorcontrib><creatorcontrib>Kanzaki, Hiroyuki</creatorcontrib><creatorcontrib>Matsumura, Hideo</creatorcontrib><creatorcontrib>Saitoh, Hiromasa</creatorcontrib><creatorcontrib>Fujisawa, Shizuko</creatorcontrib><creatorcontrib>Kamoun, Sophien</creatorcontrib><creatorcontrib>Terauchi, Ryohei</creatorcontrib><title>high-throughput screen of cell-death-inducing factors in Nicotiana benthamiana identifies a novel MAPKK that mediates INF1-induced cell death signaling and non-host resistance to Pseudomonas cichorii</title><title>The Plant journal : for cell and molecular biology</title><addtitle>Plant J</addtitle><description>A high-throughput overexpression screen of Nicotiana benthamiana cDNAs identified a gene for a mitogen-activated protein kinase kinase (MAPKK) as a potent inducer of the hypersensitive response (HR)-like cell death. NbMKK1 protein is localized to the nucleus, and the N-terminal putative MAPK docking site of NbMKK1 is required for its function as a cell-death inducer. NbMKK1-mediated leaf-cell death was compromised in leaves where NbSIPK expression was silenced by virus-induced gene silencing. A yeast two-hybrid assay showed that NbMKK1 and NbSIPK physically interact, suggesting that NbSIPK is one of the downstream targets of NbMKK1. Phytophthora infestans INF1 elicitor-mediated HR was delayed in NbMKK1-silenced plants, indicating that NbMKK1 is involved in this HR pathway. Furthermore, the resistance of N. benthamiana to a non-host pathogen Pseudomonas cichorii was compromised in NbMKK1-silenced plants. These results demonstrate that MAPK cascades involving NbMKK1 control non-host resistance including HR cell death.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Apoptosis</subject><subject>Biological and medical sciences</subject><subject>Botany</subject><subject>cell death</subject><subject>Cell Death - physiology</subject><subject>Cell Nucleus - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Genetics and breeding of economic plants</subject><subject>Hydrogen Peroxide - metabolism</subject><subject>Immunity, Innate - physiology</subject><subject>Kinases</subject><subject>MAP Kinase Signaling System - physiology</subject><subject>mitogen-activated protein kinase kinase</subject><subject>Mitogen-Activated Protein Kinase Kinases - physiology</subject><subject>Nicotiana</subject><subject>non-host resistance</subject><subject>nuclear localization</subject><subject>Pest resistance</subject><subject>Phytophthora infestans</subject><subject>Plant Diseases</subject><subject>Plant pathogens</subject><subject>Plant resistance</subject><subject>Proteins</subject><subject>Pseudomonas - physiology</subject><subject>Signal transduction</subject><subject>Signal Transduction - physiology</subject><subject>Varietal selection. Specialized plant breeding, plant breeding aims</subject><subject>virus-induced gene silencing</subject><subject>Yeast</subject><issn>0960-7412</issn><issn>1365-313X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNks9uEzEQxlcIREvhFcBC4rjBf3adzYFDVVEoLSUSQeJmzXrHWUcbO7W90D4hr4U3iegVX8bW_L6Z0XwuCsLojOXzfjNjQtalYOLnjFMqZ1RQzmf3T4rTf4mnxSldSFrOK8ZPihcxbihlcyGr58VJjmzRVPK0-NPbdV-mPvhx3e_GRKIOiI54QzQOQ9khpL60rhu1dWtiQCcfIrGO3FrtkwUHpEWXetju77bLD2ssRgLE-V84kK_ny-trkolEtthZSDl3dXvJDlWx2zci-0Yk2rWDYeoErst6V_Y-JhIw2pjAaSTJk2XEsfNb7yASbXXvg7Uvi2cGhoivjvGsWF1-XF18Lm--fbq6OL8pdVU1vOQAjcGqozCvFryiKFHKVgtjuGjAyI42ujaADUfeAWvrFqCjnM1R1qyV4qx4eyi7C_5uxJjUxo8hjxwVZ6Ja0AWtMtQcIB18jAGN2gW7hfCgGFWTf2qjJpvUZJOa_FN7_9R9lr4-1h_bvKxH4dGwDLw7AhA1DCbkpdj4yDV1I2pWZ-7DgfttB3z47wHUavllumX9m4PegFewDrnHj--cMkHpvObTR_oLENrEEw</recordid><startdate>200703</startdate><enddate>200703</enddate><creator>Takahashi, Yoshihiro</creator><creator>Nasir, Khairun Hisam Bin</creator><creator>Ito, Akiko</creator><creator>Kanzaki, Hiroyuki</creator><creator>Matsumura, Hideo</creator><creator>Saitoh, Hiromasa</creator><creator>Fujisawa, Shizuko</creator><creator>Kamoun, Sophien</creator><creator>Terauchi, Ryohei</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><general>Blackwell Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>7QR</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>200703</creationdate><title>high-throughput screen of cell-death-inducing factors in Nicotiana benthamiana identifies a novel MAPKK that mediates INF1-induced cell death signaling and non-host resistance to Pseudomonas cichorii</title><author>Takahashi, Yoshihiro ; Nasir, Khairun Hisam Bin ; Ito, Akiko ; Kanzaki, Hiroyuki ; Matsumura, Hideo ; Saitoh, Hiromasa ; Fujisawa, Shizuko ; Kamoun, Sophien ; Terauchi, Ryohei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4482-2aa8fe4d0a749240e6e66bc3ff238af6d08c5fae82e2da1b5baad0217e651b63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Apoptosis</topic><topic>Biological and medical sciences</topic><topic>Botany</topic><topic>cell death</topic><topic>Cell Death - physiology</topic><topic>Cell Nucleus - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Genetics and breeding of economic plants</topic><topic>Hydrogen Peroxide - metabolism</topic><topic>Immunity, Innate - physiology</topic><topic>Kinases</topic><topic>MAP Kinase Signaling System - physiology</topic><topic>mitogen-activated protein kinase kinase</topic><topic>Mitogen-Activated Protein Kinase Kinases - physiology</topic><topic>Nicotiana</topic><topic>non-host resistance</topic><topic>nuclear localization</topic><topic>Pest resistance</topic><topic>Phytophthora infestans</topic><topic>Plant Diseases</topic><topic>Plant pathogens</topic><topic>Plant resistance</topic><topic>Proteins</topic><topic>Pseudomonas - physiology</topic><topic>Signal transduction</topic><topic>Signal Transduction - physiology</topic><topic>Varietal selection. Specialized plant breeding, plant breeding aims</topic><topic>virus-induced gene silencing</topic><topic>Yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Takahashi, Yoshihiro</creatorcontrib><creatorcontrib>Nasir, Khairun Hisam Bin</creatorcontrib><creatorcontrib>Ito, Akiko</creatorcontrib><creatorcontrib>Kanzaki, Hiroyuki</creatorcontrib><creatorcontrib>Matsumura, Hideo</creatorcontrib><creatorcontrib>Saitoh, Hiromasa</creatorcontrib><creatorcontrib>Fujisawa, Shizuko</creatorcontrib><creatorcontrib>Kamoun, Sophien</creatorcontrib><creatorcontrib>Terauchi, Ryohei</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>The Plant journal : for cell and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Takahashi, Yoshihiro</au><au>Nasir, Khairun Hisam Bin</au><au>Ito, Akiko</au><au>Kanzaki, Hiroyuki</au><au>Matsumura, Hideo</au><au>Saitoh, Hiromasa</au><au>Fujisawa, Shizuko</au><au>Kamoun, Sophien</au><au>Terauchi, Ryohei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>high-throughput screen of cell-death-inducing factors in Nicotiana benthamiana identifies a novel MAPKK that mediates INF1-induced cell death signaling and non-host resistance to Pseudomonas cichorii</atitle><jtitle>The Plant journal : for cell and molecular biology</jtitle><addtitle>Plant J</addtitle><date>2007-03</date><risdate>2007</risdate><volume>49</volume><issue>6</issue><spage>1030</spage><epage>1040</epage><pages>1030-1040</pages><issn>0960-7412</issn><eissn>1365-313X</eissn><abstract>A high-throughput overexpression screen of Nicotiana benthamiana cDNAs identified a gene for a mitogen-activated protein kinase kinase (MAPKK) as a potent inducer of the hypersensitive response (HR)-like cell death. NbMKK1 protein is localized to the nucleus, and the N-terminal putative MAPK docking site of NbMKK1 is required for its function as a cell-death inducer. NbMKK1-mediated leaf-cell death was compromised in leaves where NbSIPK expression was silenced by virus-induced gene silencing. A yeast two-hybrid assay showed that NbMKK1 and NbSIPK physically interact, suggesting that NbSIPK is one of the downstream targets of NbMKK1. Phytophthora infestans INF1 elicitor-mediated HR was delayed in NbMKK1-silenced plants, indicating that NbMKK1 is involved in this HR pathway. Furthermore, the resistance of N. benthamiana to a non-host pathogen Pseudomonas cichorii was compromised in NbMKK1-silenced plants. These results demonstrate that MAPK cascades involving NbMKK1 control non-host resistance including HR cell death.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>17319846</pmid><doi>10.1111/j.1365-313X.2006.03022.x</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0960-7412 |
| ispartof | The Plant journal : for cell and molecular biology, 2007-03, Vol.49 (6), p.1030-1040 |
| issn | 0960-7412 1365-313X |
| language | eng |
| recordid | cdi_proquest_journals_213490904 |
| source | Wiley Free Content; MEDLINE; IngentaConnect Free/Open Access Journals; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Agronomy. Soil science and plant productions Apoptosis Biological and medical sciences Botany cell death Cell Death - physiology Cell Nucleus - metabolism Fundamental and applied biological sciences. Psychology Gene Expression Genetics and breeding of economic plants Hydrogen Peroxide - metabolism Immunity, Innate - physiology Kinases MAP Kinase Signaling System - physiology mitogen-activated protein kinase kinase Mitogen-Activated Protein Kinase Kinases - physiology Nicotiana non-host resistance nuclear localization Pest resistance Phytophthora infestans Plant Diseases Plant pathogens Plant resistance Proteins Pseudomonas - physiology Signal transduction Signal Transduction - physiology Varietal selection. Specialized plant breeding, plant breeding aims virus-induced gene silencing Yeast |
| title | high-throughput screen of cell-death-inducing factors in Nicotiana benthamiana identifies a novel MAPKK that mediates INF1-induced cell death signaling and non-host resistance to Pseudomonas cichorii |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-13T09%3A04%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=high-throughput%20screen%20of%20cell-death-inducing%20factors%20in%20Nicotiana%20benthamiana%20identifies%20a%20novel%20MAPKK%20that%20mediates%20INF1-induced%20cell%20death%20signaling%20and%20non-host%20resistance%20to%20Pseudomonas%20cichorii&rft.jtitle=The%20Plant%20journal%20:%20for%20cell%20and%20molecular%20biology&rft.au=Takahashi,%20Yoshihiro&rft.date=2007-03&rft.volume=49&rft.issue=6&rft.spage=1030&rft.epage=1040&rft.pages=1030-1040&rft.issn=0960-7412&rft.eissn=1365-313X&rft_id=info:doi/10.1111/j.1365-313X.2006.03022.x&rft_dat=%3Cproquest_cross%3E1227397011%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=213490904&rft_id=info:pmid/17319846&rfr_iscdi=true |