Effect of Insulin on Ca2+-Dependent Hyperpolarization in Erythrocytes from Healthy Donors and Patients with Type 2 Diabetes Mellitus Accompanied by Arterial Hypertension

Issue Title: This issue is a translation of Byulleten' Eksperimental'noi Biologii i Meditsiny (Bulletin of Experimental Biology and Medicine) and Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine) Insulin decreased A23187-induced hyperpolarization of the erythrocyte membrane in healthy donors. These data indicate that insulin plays a role in the regulation of Ca2+-activated potassium channels in human erythrocytes. However, insulin had little effect on hyperpolarization response of cells induced by artificial ascorbate--phenazine methosulfate donor-acceptor system. Addition of insulin to cell suspension from patients with type 2 diabetes mellitus did not modulate hyperpolarization of the erythrocyte membrane induced by A23187 or ascorbate-phenazine methosulfate, which reflects impairment of regulatory mechanisms for Ca 2+-activated potassium channels in erythrocytes.