Effect of Semax peptide on survival of cultured rat pheochromocytoma cells during oxidative stress
We studied the effects of Semax (antiinsulin peptide with neuroprotective effect) on the survival of cultured rat pheochromocytoma cell after oxidative stress induced by short-term incubation with hydrogen peroxide. Studies with fluorescent dyes propidium iodide and Hoechst 33258 showed that cell in...
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| Veröffentlicht in: | Bulletin of experimental biology and medicine 2003-03, Vol.135 (3), p.268 |
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| creator | Safarova, E R Shram, S I Zolotarev, Yu A Myasoedov, N F |
| description | We studied the effects of Semax (antiinsulin peptide with neuroprotective effect) on the survival of cultured rat pheochromocytoma cell after oxidative stress induced by short-term incubation with hydrogen peroxide. Studies with fluorescent dyes propidium iodide and Hoechst 33258 showed that cell incubation with hydrogen peroxide led to the formation of damaged cells with characteristic signs of necrosis. Semax dose-dependently reduced the number of cells damaged by oxidative stress. The efficiency of Semax depended on the time of its addition to the culture medium. The results suggest that the neuroprotective effect of Semax in ischemic stroke can be due to its capacity to protect neurons from damage caused by oxidative stress. |
| doi_str_mv | 10.1023/A:1024141232307 |
| format | Article |
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Studies with fluorescent dyes propidium iodide and Hoechst 33258 showed that cell incubation with hydrogen peroxide led to the formation of damaged cells with characteristic signs of necrosis. Semax dose-dependently reduced the number of cells damaged by oxidative stress. The efficiency of Semax depended on the time of its addition to the culture medium. The results suggest that the neuroprotective effect of Semax in ischemic stroke can be due to its capacity to protect neurons from damage caused by oxidative stress.</description><identifier>ISSN: 0007-4888</identifier><identifier>EISSN: 1573-8221</identifier><identifier>DOI: 10.1023/A:1024141232307</identifier><identifier>PMID: 12802399</identifier><identifier>CODEN: BEXBAN</identifier><language>eng</language><publisher>United States: Springer Nature B.V</publisher><subject>Adrenocorticotropic Hormone - analogs & derivatives ; Adrenocorticotropic Hormone - pharmacology ; Animals ; Cell Survival - drug effects ; Dose-Response Relationship, Drug ; Fluorescent Dyes - metabolism ; Hydrogen Peroxide - pharmacology ; Necrosis ; Neuroprotective Agents - pharmacology ; Oxidants - pharmacology ; Oxidative Stress ; PC12 Cells - cytology ; PC12 Cells - drug effects ; Peptide Fragments - pharmacology ; Rats</subject><ispartof>Bulletin of experimental biology and medicine, 2003-03, Vol.135 (3), p.268</ispartof><rights>Copyright (c) 2003 Plenum Publishing Corporation</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c280t-66f9373a41f5b7bed21760bddfb75c851e76f1a56a7882020ed361878184efe53</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12802399$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Safarova, E R</creatorcontrib><creatorcontrib>Shram, S I</creatorcontrib><creatorcontrib>Zolotarev, Yu A</creatorcontrib><creatorcontrib>Myasoedov, N F</creatorcontrib><title>Effect of Semax peptide on survival of cultured rat pheochromocytoma cells during oxidative stress</title><title>Bulletin of experimental biology and medicine</title><addtitle>Bull Exp Biol Med</addtitle><description>We studied the effects of Semax (antiinsulin peptide with neuroprotective effect) on the survival of cultured rat pheochromocytoma cell after oxidative stress induced by short-term incubation with hydrogen peroxide. 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The results suggest that the neuroprotective effect of Semax in ischemic stroke can be due to its capacity to protect neurons from damage caused by oxidative stress.</description><subject>Adrenocorticotropic Hormone - analogs & derivatives</subject><subject>Adrenocorticotropic Hormone - pharmacology</subject><subject>Animals</subject><subject>Cell Survival - drug effects</subject><subject>Dose-Response Relationship, Drug</subject><subject>Fluorescent Dyes - metabolism</subject><subject>Hydrogen Peroxide - pharmacology</subject><subject>Necrosis</subject><subject>Neuroprotective Agents - pharmacology</subject><subject>Oxidants - pharmacology</subject><subject>Oxidative Stress</subject><subject>PC12 Cells - cytology</subject><subject>PC12 Cells - drug effects</subject><subject>Peptide Fragments - pharmacology</subject><subject>Rats</subject><issn>0007-4888</issn><issn>1573-8221</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNo1kEtPwzAQhC0EoqVw5oYs7gGvncQOt6oqD6kSB-AcOfGapkrqYDtV--9JRTl9Wu3s7GgIuQX2AIyLx_nTiBRS4IILJs_IFDIpEsU5nJMpY0wmqVJqQq5C2BxHlsMlmQBX43VRTEm1tBbrSJ2lH9jpPe2xj41B6rY0DH7X7HR7XNZDGwePhnodab9GV6-961x9iK7TtMa2DdQMvtl-U7dvjI7NDmmIHkO4JhdWtwFvTpyRr-fl5-I1Wb2_vC3mq6Qe08Qkz20hpNAp2KySFRoOMmeVMbaSWa0yQJlb0FmupVKccYZG5KCkApWixUzMyP2fb-_dz4Ahlhs3-O34suQgRM55AaPo7iQaqg5N2fum0_5Q_jcifgF4bWMy</recordid><startdate>200303</startdate><enddate>200303</enddate><creator>Safarova, E R</creator><creator>Shram, S I</creator><creator>Zolotarev, Yu A</creator><creator>Myasoedov, N F</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>200303</creationdate><title>Effect of Semax peptide on survival of cultured rat pheochromocytoma cells during oxidative stress</title><author>Safarova, E R ; 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Studies with fluorescent dyes propidium iodide and Hoechst 33258 showed that cell incubation with hydrogen peroxide led to the formation of damaged cells with characteristic signs of necrosis. Semax dose-dependently reduced the number of cells damaged by oxidative stress. The efficiency of Semax depended on the time of its addition to the culture medium. The results suggest that the neuroprotective effect of Semax in ischemic stroke can be due to its capacity to protect neurons from damage caused by oxidative stress.</abstract><cop>United States</cop><pub>Springer Nature B.V</pub><pmid>12802399</pmid><doi>10.1023/A:1024141232307</doi></addata></record> |
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| subjects | Adrenocorticotropic Hormone - analogs & derivatives Adrenocorticotropic Hormone - pharmacology Animals Cell Survival - drug effects Dose-Response Relationship, Drug Fluorescent Dyes - metabolism Hydrogen Peroxide - pharmacology Necrosis Neuroprotective Agents - pharmacology Oxidants - pharmacology Oxidative Stress PC12 Cells - cytology PC12 Cells - drug effects Peptide Fragments - pharmacology Rats |
| title | Effect of Semax peptide on survival of cultured rat pheochromocytoma cells during oxidative stress |
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