Transferability of Simple Sequence Repeat (SSR) Markers Developed in Red Clover (Trifolium pratense L.) to Some Trifolium Species
Microsatellite markers previously developed for red clover ( Trifolium pratense L.) were used in cross-amplification tests of eight other Trifolium species. Of the 20 SSR loci tested, 9 (47.37 %) had positive results and were found to be transferable to all eight species examined. The number of alle...
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Veröffentlicht in: | Iranian journal of science and technology. Transaction A, Science Science, 2016-03, Vol.40 (1), p.59-62 |
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creator | Haerinasab, M. Rahiminejad, M. R. Ellison, N. W. |
description | Microsatellite markers previously developed for red clover (
Trifolium pratense
L.) were used in cross-amplification tests of eight other
Trifolium
species. Of the 20 SSR loci tested, 9 (47.37 %) had positive results and were found to be transferable to all eight species examined. The number of alleles detected at each locus ranged from 4 to 12, with an average of 7.2 in
T. tumens
Steven ex M. Bieb., 5.8 in
T. resupinatum
L., 5.1 in
T. fragiferum
L., 4.5 in
T. tomentosum
L., 3.4 in
T. bullatum
Boiss. & Hausskn. ex Boiss., 3.2 in
T. clusii
Godr. & Gren., 2.7 in
T. spumosum
L. and 2.1 in
T. physodes
Stev. ex M. B. This study has shown that SSR markers developed for red clover effectively amplify DNA from other species and this approach may be applicable for the analysis of intra- and interspecific genetic diversity of target
Trifolium
species. |
doi_str_mv | 10.1007/s40995-016-0011-1 |
format | Article |
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Trifolium pratense
L.) were used in cross-amplification tests of eight other
Trifolium
species. Of the 20 SSR loci tested, 9 (47.37 %) had positive results and were found to be transferable to all eight species examined. The number of alleles detected at each locus ranged from 4 to 12, with an average of 7.2 in
T. tumens
Steven ex M. Bieb., 5.8 in
T. resupinatum
L., 5.1 in
T. fragiferum
L., 4.5 in
T. tomentosum
L., 3.4 in
T. bullatum
Boiss. & Hausskn. ex Boiss., 3.2 in
T. clusii
Godr. & Gren., 2.7 in
T. spumosum
L. and 2.1 in
T. physodes
Stev. ex M. B. This study has shown that SSR markers developed for red clover effectively amplify DNA from other species and this approach may be applicable for the analysis of intra- and interspecific genetic diversity of target
Trifolium
species.</description><identifier>ISSN: 1028-6276</identifier><identifier>EISSN: 2364-1819</identifier><identifier>DOI: 10.1007/s40995-016-0011-1</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Biodiversity ; Chemistry/Food Science ; Clover ; Deoxyribonucleic acid ; DNA ; Earth Sciences ; Engineering ; Genetic analysis ; Genetic diversity ; Genetic markers ; Genomes ; Interspecific ; Life Sciences ; Loci ; Markers ; Materials Science ; Microsatellites ; Physics ; Red clover ; Research Paper ; Species ; Species diversity ; Trifolium ; Trifolium pratense</subject><ispartof>Iranian journal of science and technology. Transaction A, Science, 2016-03, Vol.40 (1), p.59-62</ispartof><rights>Shiraz University 2016</rights><rights>Copyright Springer Science & Business Media 2016</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-4ae3b72fdeec0045a1d31ec011557f232bdecc35bddd105e05506c73e11748ea3</citedby><cites>FETCH-LOGICAL-c316t-4ae3b72fdeec0045a1d31ec011557f232bdecc35bddd105e05506c73e11748ea3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Haerinasab, M.</creatorcontrib><creatorcontrib>Rahiminejad, M. R.</creatorcontrib><creatorcontrib>Ellison, N. W.</creatorcontrib><title>Transferability of Simple Sequence Repeat (SSR) Markers Developed in Red Clover (Trifolium pratense L.) to Some Trifolium Species</title><title>Iranian journal of science and technology. Transaction A, Science</title><addtitle>Iran. J. Sci. Technol. Trans. Sci</addtitle><description>Microsatellite markers previously developed for red clover (
Trifolium pratense
L.) were used in cross-amplification tests of eight other
Trifolium
species. Of the 20 SSR loci tested, 9 (47.37 %) had positive results and were found to be transferable to all eight species examined. The number of alleles detected at each locus ranged from 4 to 12, with an average of 7.2 in
T. tumens
Steven ex M. Bieb., 5.8 in
T. resupinatum
L., 5.1 in
T. fragiferum
L., 4.5 in
T. tomentosum
L., 3.4 in
T. bullatum
Boiss. & Hausskn. ex Boiss., 3.2 in
T. clusii
Godr. & Gren., 2.7 in
T. spumosum
L. and 2.1 in
T. physodes
Stev. ex M. B. This study has shown that SSR markers developed for red clover effectively amplify DNA from other species and this approach may be applicable for the analysis of intra- and interspecific genetic diversity of target
Trifolium
species.</description><subject>Biodiversity</subject><subject>Chemistry/Food Science</subject><subject>Clover</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Earth Sciences</subject><subject>Engineering</subject><subject>Genetic analysis</subject><subject>Genetic diversity</subject><subject>Genetic markers</subject><subject>Genomes</subject><subject>Interspecific</subject><subject>Life Sciences</subject><subject>Loci</subject><subject>Markers</subject><subject>Materials Science</subject><subject>Microsatellites</subject><subject>Physics</subject><subject>Red clover</subject><subject>Research Paper</subject><subject>Species</subject><subject>Species diversity</subject><subject>Trifolium</subject><subject>Trifolium pratense</subject><issn>1028-6276</issn><issn>2364-1819</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp1kE9rwzAMxc3YYKXrB9jNsEt7SGfZcZIeR_cXOgZNdzZOogxvaZzZaaHHffO5ZNDTLpJA7z2hHyHXwObAWHrrY7ZYyIhBEjEGEMEZGXGRxBFksDgnI2A8ixKeJpdk4r0pmABIUh4nI_Kzcbr1NTpdmMb0B2prmptt1yDN8XuHbYl0jR3qnk7zfD2jr9p9ofP0HvfY2A4ratqgqOiysXt0dLpxpraN2W1p53SPrUe6ms9ob2lut0hP67zD0qC_Ihe1bjxO_vqYvD8-bJbP0ert6WV5t4pKAUkfxRpFkfK6QiwZi6WGSkAYAaRMay54UWFZCllUVQVMIpOSJWUqECCNM9RiTG6G3M7Z8Jjv1afduTacVByEEGnGQh0TGFSls947rFXnzFa7gwKmjrDVAFsF2OoIW0Hw8MHjg7b9QHdK_t_0C76VgcQ</recordid><startdate>20160301</startdate><enddate>20160301</enddate><creator>Haerinasab, M.</creator><creator>Rahiminejad, M. 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R. ; Ellison, N. 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Transaction A, Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Haerinasab, M.</au><au>Rahiminejad, M. R.</au><au>Ellison, N. W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transferability of Simple Sequence Repeat (SSR) Markers Developed in Red Clover (Trifolium pratense L.) to Some Trifolium Species</atitle><jtitle>Iranian journal of science and technology. Transaction A, Science</jtitle><stitle>Iran. J. Sci. Technol. Trans. Sci</stitle><date>2016-03-01</date><risdate>2016</risdate><volume>40</volume><issue>1</issue><spage>59</spage><epage>62</epage><pages>59-62</pages><issn>1028-6276</issn><eissn>2364-1819</eissn><abstract>Microsatellite markers previously developed for red clover (
Trifolium pratense
L.) were used in cross-amplification tests of eight other
Trifolium
species. Of the 20 SSR loci tested, 9 (47.37 %) had positive results and were found to be transferable to all eight species examined. The number of alleles detected at each locus ranged from 4 to 12, with an average of 7.2 in
T. tumens
Steven ex M. Bieb., 5.8 in
T. resupinatum
L., 5.1 in
T. fragiferum
L., 4.5 in
T. tomentosum
L., 3.4 in
T. bullatum
Boiss. & Hausskn. ex Boiss., 3.2 in
T. clusii
Godr. & Gren., 2.7 in
T. spumosum
L. and 2.1 in
T. physodes
Stev. ex M. B. This study has shown that SSR markers developed for red clover effectively amplify DNA from other species and this approach may be applicable for the analysis of intra- and interspecific genetic diversity of target
Trifolium
species.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><doi>10.1007/s40995-016-0011-1</doi><tpages>4</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1028-6276 |
ispartof | Iranian journal of science and technology. Transaction A, Science, 2016-03, Vol.40 (1), p.59-62 |
issn | 1028-6276 2364-1819 |
language | eng |
recordid | cdi_proquest_journals_2133378033 |
source | Alma/SFX Local Collection |
subjects | Biodiversity Chemistry/Food Science Clover Deoxyribonucleic acid DNA Earth Sciences Engineering Genetic analysis Genetic diversity Genetic markers Genomes Interspecific Life Sciences Loci Markers Materials Science Microsatellites Physics Red clover Research Paper Species Species diversity Trifolium Trifolium pratense |
title | Transferability of Simple Sequence Repeat (SSR) Markers Developed in Red Clover (Trifolium pratense L.) to Some Trifolium Species |
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