Transferability of Simple Sequence Repeat (SSR) Markers Developed in Red Clover (Trifolium pratense L.) to Some Trifolium Species

Microsatellite markers previously developed for red clover ( Trifolium pratense L.) were used in cross-amplification tests of eight other Trifolium species. Of the 20 SSR loci tested, 9 (47.37 %) had positive results and were found to be transferable to all eight species examined. The number of alle...

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Veröffentlicht in:Iranian journal of science and technology. Transaction A, Science Science, 2016-03, Vol.40 (1), p.59-62
Hauptverfasser: Haerinasab, M., Rahiminejad, M. R., Ellison, N. W.
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container_title Iranian journal of science and technology. Transaction A, Science
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creator Haerinasab, M.
Rahiminejad, M. R.
Ellison, N. W.
description Microsatellite markers previously developed for red clover ( Trifolium pratense L.) were used in cross-amplification tests of eight other Trifolium species. Of the 20 SSR loci tested, 9 (47.37 %) had positive results and were found to be transferable to all eight species examined. The number of alleles detected at each locus ranged from 4 to 12, with an average of 7.2 in T. tumens Steven ex M. Bieb., 5.8 in T. resupinatum L., 5.1 in T. fragiferum L., 4.5 in T. tomentosum L., 3.4 in T. bullatum Boiss. & Hausskn. ex Boiss., 3.2 in T. clusii Godr. & Gren., 2.7 in T. spumosum L. and 2.1 in T. physodes Stev. ex M. B. This study has shown that SSR markers developed for red clover effectively amplify DNA from other species and this approach may be applicable for the analysis of intra- and interspecific genetic diversity of target Trifolium species.
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identifier ISSN: 1028-6276
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subjects Biodiversity
Chemistry/Food Science
Clover
Deoxyribonucleic acid
DNA
Earth Sciences
Engineering
Genetic analysis
Genetic diversity
Genetic markers
Genomes
Interspecific
Life Sciences
Loci
Markers
Materials Science
Microsatellites
Physics
Red clover
Research Paper
Species
Species diversity
Trifolium
Trifolium pratense
title Transferability of Simple Sequence Repeat (SSR) Markers Developed in Red Clover (Trifolium pratense L.) to Some Trifolium Species
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