Efficient direct plant regeneration from immature leaf roll explants of sugarcane (Saccharum officinarum L.) using polyamines and assessment of genetic fidelity by SCoT markers
A rapid and efficient method for in vitro direct plant regeneration from immature leaf roll explants of Saccharum officinarum L. (sugarcane) cv. Co 86032 was developed by the application of exogenous polyamines (PA). The effect of explant source from apical meristems and pre-culture of explants in t...
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| Veröffentlicht in: | In vitro cellular & developmental biology. Plant 2018-08, Vol.54 (4), p.399-412 |
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| container_title | In vitro cellular & developmental biology. Plant |
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| creator | Sathish, Dorairaj Vasudevan, Venkatachalam Theboral, Jeevaraj Elayaraja, Dhandapani Appunu, Chinnaswamy Siva, Ramamoorthy Manickavasagam, Markandan |
| description | A rapid and efficient method for in vitro direct plant regeneration from immature leaf roll explants of Saccharum officinarum L. (sugarcane) cv. Co 86032 was developed by the application of exogenous polyamines (PA). The effect of explant source from apical meristems and pre-culture of explants in the dark on shoot regeneration was studied. Adventitious shoot regeneration occurred on the proximal regions of immature leaf roll explants when pre-incubated in the dark for 2 wk and the regeneration response was decreased from the middle to distal end. A higher number of direct shoots (130 primary shoots explant−1) and multiple shoots (657 secondary shoots explant−1), were obtained with a combination of spermidine (103.27 μM), spermine (49.42 μM), and putrescine (31.04 μM) along with plant growth regulators. Shoot induction was increased up to twofold and multiplication was increased up to threefold in the medium supplemented with PA. Profuse rooting was observed in putrescine (93.12 μM), spermidine (68.84 μM), and spermine (24.71 μM), with mean number of 57 roots. A twofold increase in the number of roots was observed in medium supplemented with PA with respect to control cultures, which facilitated the successful transplantation and acclimatization process of in vitro propagated sugarcane plants. Histology and scanning electron microscopy analyses supported adventitious direct shoot regeneration from immature leaf roll explants. The genetic stability of in vitro regenerated plants was confirmed using start codon targeted polymorphism marker system. |
| doi_str_mv | 10.1007/s11627-018-9910-5 |
| format | Article |
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(sugarcane) cv. Co 86032 was developed by the application of exogenous polyamines (PA). The effect of explant source from apical meristems and pre-culture of explants in the dark on shoot regeneration was studied. Adventitious shoot regeneration occurred on the proximal regions of immature leaf roll explants when pre-incubated in the dark for 2 wk and the regeneration response was decreased from the middle to distal end. A higher number of direct shoots (130 primary shoots explant−1) and multiple shoots (657 secondary shoots explant−1), were obtained with a combination of spermidine (103.27 μM), spermine (49.42 μM), and putrescine (31.04 μM) along with plant growth regulators. Shoot induction was increased up to twofold and multiplication was increased up to threefold in the medium supplemented with PA. Profuse rooting was observed in putrescine (93.12 μM), spermidine (68.84 μM), and spermine (24.71 μM), with mean number of 57 roots. A twofold increase in the number of roots was observed in medium supplemented with PA with respect to control cultures, which facilitated the successful transplantation and acclimatization process of in vitro propagated sugarcane plants. Histology and scanning electron microscopy analyses supported adventitious direct shoot regeneration from immature leaf roll explants. The genetic stability of in vitro regenerated plants was confirmed using start codon targeted polymorphism marker system.</description><identifier>ISSN: 1054-5476</identifier><identifier>EISSN: 1475-2689</identifier><identifier>DOI: 10.1007/s11627-018-9910-5</identifier><language>eng</language><publisher>New York: Springer Science + Business Media, LLC (Springer)</publisher><subject>Acclimatization ; Agricultural commodities ; Biomedical and Life Sciences ; Cell Biology ; Cell division ; Deoxyribonucleic acid ; Developmental Biology ; DNA ; Explants ; Growth regulators ; Histology ; In vitro methods and tests ; Leaves ; Life Sciences ; Meristems ; MICROPROPAGATION ; Morphogenesis ; Plant Breeding/Biotechnology ; Plant Genetics and Genomics ; Plant growth ; Plant Sciences ; Plants ; Polyamines ; Polymorphism ; Putrescine ; Regeneration ; Rooting ; Roots ; Saccharum officinarum ; Scanning electron microscopy ; Shoots ; Spermidine ; Spermine ; Sugarcane ; Transplantation</subject><ispartof>In vitro cellular & developmental biology. 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Plant</title><addtitle>In Vitro Cell.Dev.Biol.-Plant</addtitle><description>A rapid and efficient method for in vitro direct plant regeneration from immature leaf roll explants of Saccharum officinarum L. (sugarcane) cv. Co 86032 was developed by the application of exogenous polyamines (PA). The effect of explant source from apical meristems and pre-culture of explants in the dark on shoot regeneration was studied. Adventitious shoot regeneration occurred on the proximal regions of immature leaf roll explants when pre-incubated in the dark for 2 wk and the regeneration response was decreased from the middle to distal end. A higher number of direct shoots (130 primary shoots explant−1) and multiple shoots (657 secondary shoots explant−1), were obtained with a combination of spermidine (103.27 μM), spermine (49.42 μM), and putrescine (31.04 μM) along with plant growth regulators. Shoot induction was increased up to twofold and multiplication was increased up to threefold in the medium supplemented with PA. Profuse rooting was observed in putrescine (93.12 μM), spermidine (68.84 μM), and spermine (24.71 μM), with mean number of 57 roots. A twofold increase in the number of roots was observed in medium supplemented with PA with respect to control cultures, which facilitated the successful transplantation and acclimatization process of in vitro propagated sugarcane plants. Histology and scanning electron microscopy analyses supported adventitious direct shoot regeneration from immature leaf roll explants. The genetic stability of in vitro regenerated plants was confirmed using start codon targeted polymorphism marker system.</description><subject>Acclimatization</subject><subject>Agricultural commodities</subject><subject>Biomedical and Life Sciences</subject><subject>Cell Biology</subject><subject>Cell division</subject><subject>Deoxyribonucleic acid</subject><subject>Developmental Biology</subject><subject>DNA</subject><subject>Explants</subject><subject>Growth regulators</subject><subject>Histology</subject><subject>In vitro methods and tests</subject><subject>Leaves</subject><subject>Life Sciences</subject><subject>Meristems</subject><subject>MICROPROPAGATION</subject><subject>Morphogenesis</subject><subject>Plant Breeding/Biotechnology</subject><subject>Plant Genetics and Genomics</subject><subject>Plant growth</subject><subject>Plant Sciences</subject><subject>Plants</subject><subject>Polyamines</subject><subject>Polymorphism</subject><subject>Putrescine</subject><subject>Regeneration</subject><subject>Rooting</subject><subject>Roots</subject><subject>Saccharum officinarum</subject><subject>Scanning electron microscopy</subject><subject>Shoots</subject><subject>Spermidine</subject><subject>Spermine</subject><subject>Sugarcane</subject><subject>Transplantation</subject><issn>1054-5476</issn><issn>1475-2689</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9Uctu1DAUjRBIlNIPYIFkiQ0s0vra4zhZolF5SCN10XZteezrwUNiD7YjMX_FJ-I0CHas7kPnJZ2meQP0GiiVNxmgY7Kl0LfDALQVz5oL2EjRsq4fntedik0rNrJ72bzK-UgpBQryovl165w3HkMh1ic0hZxGXY-EBwyYdPExEJfiRPw06TInJCNqR1IcR4I_n8CZREfyfNDJ6IDk_b025ptO81T_i3h42nfXH8icfTiQUxzPevIBM9HBEp0z5jwtEarOYlu8Ic5bHH05k_2Z3G_jA5l0-o4pv25eOD1mvPozL5vHT7cP2y_t7u7z1-3HXWs470srYS-dM9ZazY2Udi-l2dtBWBSyt4Y6vsG-Z5pzxiVI6agDjkZSasBRRvll827VPaX4Y8Zc1DHOKVRLxYAz0XEQsqJgRZkUc07o1Cn5mvSsgKqlGLUWo2oxailGicphKydXbDhg-qf8P9LblXTMJaa_LqwTAwUQ_DcbLZ5R</recordid><startdate>20180801</startdate><enddate>20180801</enddate><creator>Sathish, Dorairaj</creator><creator>Vasudevan, Venkatachalam</creator><creator>Theboral, Jeevaraj</creator><creator>Elayaraja, Dhandapani</creator><creator>Appunu, Chinnaswamy</creator><creator>Siva, Ramamoorthy</creator><creator>Manickavasagam, Markandan</creator><general>Springer Science + Business Media, LLC (Springer)</general><general>Springer US</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4T-</scope><scope>4U-</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>S0X</scope></search><sort><creationdate>20180801</creationdate><title>Efficient direct plant regeneration from immature leaf roll explants of sugarcane (Saccharum officinarum L.) using polyamines and assessment of genetic fidelity by SCoT markers</title><author>Sathish, Dorairaj ; 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Plant</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sathish, Dorairaj</au><au>Vasudevan, Venkatachalam</au><au>Theboral, Jeevaraj</au><au>Elayaraja, Dhandapani</au><au>Appunu, Chinnaswamy</au><au>Siva, Ramamoorthy</au><au>Manickavasagam, Markandan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Efficient direct plant regeneration from immature leaf roll explants of sugarcane (Saccharum officinarum L.) using polyamines and assessment of genetic fidelity by SCoT markers</atitle><jtitle>In vitro cellular & developmental biology. Plant</jtitle><stitle>In Vitro Cell.Dev.Biol.-Plant</stitle><date>2018-08-01</date><risdate>2018</risdate><volume>54</volume><issue>4</issue><spage>399</spage><epage>412</epage><pages>399-412</pages><issn>1054-5476</issn><eissn>1475-2689</eissn><abstract>A rapid and efficient method for in vitro direct plant regeneration from immature leaf roll explants of Saccharum officinarum L. (sugarcane) cv. Co 86032 was developed by the application of exogenous polyamines (PA). The effect of explant source from apical meristems and pre-culture of explants in the dark on shoot regeneration was studied. Adventitious shoot regeneration occurred on the proximal regions of immature leaf roll explants when pre-incubated in the dark for 2 wk and the regeneration response was decreased from the middle to distal end. A higher number of direct shoots (130 primary shoots explant−1) and multiple shoots (657 secondary shoots explant−1), were obtained with a combination of spermidine (103.27 μM), spermine (49.42 μM), and putrescine (31.04 μM) along with plant growth regulators. Shoot induction was increased up to twofold and multiplication was increased up to threefold in the medium supplemented with PA. Profuse rooting was observed in putrescine (93.12 μM), spermidine (68.84 μM), and spermine (24.71 μM), with mean number of 57 roots. A twofold increase in the number of roots was observed in medium supplemented with PA with respect to control cultures, which facilitated the successful transplantation and acclimatization process of in vitro propagated sugarcane plants. Histology and scanning electron microscopy analyses supported adventitious direct shoot regeneration from immature leaf roll explants. The genetic stability of in vitro regenerated plants was confirmed using start codon targeted polymorphism marker system.</abstract><cop>New York</cop><pub>Springer Science + Business Media, LLC (Springer)</pub><doi>10.1007/s11627-018-9910-5</doi><tpages>14</tpages></addata></record> |
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| ispartof | In vitro cellular & developmental biology. Plant, 2018-08, Vol.54 (4), p.399-412 |
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| subjects | Acclimatization Agricultural commodities Biomedical and Life Sciences Cell Biology Cell division Deoxyribonucleic acid Developmental Biology DNA Explants Growth regulators Histology In vitro methods and tests Leaves Life Sciences Meristems MICROPROPAGATION Morphogenesis Plant Breeding/Biotechnology Plant Genetics and Genomics Plant growth Plant Sciences Plants Polyamines Polymorphism Putrescine Regeneration Rooting Roots Saccharum officinarum Scanning electron microscopy Shoots Spermidine Spermine Sugarcane Transplantation |
| title | Efficient direct plant regeneration from immature leaf roll explants of sugarcane (Saccharum officinarum L.) using polyamines and assessment of genetic fidelity by SCoT markers |
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