Induction of liver cytochrome P450 1A2 expression by flutamide in rats

To investigate the modulation of liver cytochrome P450 1A2 (CYP1A2) expression by giving flutamide to adult rats. Rats were given 50, 100, and 200 mg/kg po of flutamide for 2 weeks. Liver CYP1A2 mRNA was measured using reverse transcription-polymerase chain reaction. CYP1A2 protein was detected usin...

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Veröffentlicht in:Acta pharmacologica Sinica 2005-11, Vol.26 (11), p.1382-1386
Hauptverfasser: Wang, Hai-xue, Liu, Xiao, Xu, Chang-jiang, Ma, Xiao-chao, Long, Jian-er, Li, Duan
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container_issue 11
container_start_page 1382
container_title Acta pharmacologica Sinica
container_volume 26
creator Wang, Hai-xue
Liu, Xiao
Xu, Chang-jiang
Ma, Xiao-chao
Long, Jian-er
Li, Duan
description To investigate the modulation of liver cytochrome P450 1A2 (CYP1A2) expression by giving flutamide to adult rats. Rats were given 50, 100, and 200 mg/kg po of flutamide for 2 weeks. Liver CYP1A2 mRNA was measured using reverse transcription-polymerase chain reaction. CYP1A2 protein was detected using immunoblotting. CYP1A2 activity was assayed using high performance liquid chromatography, with caffeine as the CYP1A2 substrate. CYP1A2 mRNA levels after flutamide treatment at 100 mg/kg and 200 mg/kg were, respectively, 1.86 and 3.11-fold higher than those of the control. Correspondingly, CYP1A2 protein increased 1.78 and 2.89-fold and CYP1A2 activity increased approximately 1.65 and 2.83-fold, respectively, relative to controls. Flutamide treatment at 50 mg/kg had no significant effect on CYP1A2 mRNA, protein, or enzyme activity. Giving rats flutamide induced liver CYP1A2 expression in a dose-dependent manner.
doi_str_mv 10.1111/j.1745-7254.2005.00211.x
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subjects Androgen Antagonists - toxicity
Animals
Cytochrome P-450 CYP1A2 - biosynthesis
Cytochrome P-450 CYP1A2 - genetics
Dose-Response Relationship, Drug
Flutamide - administration & dosage
Flutamide - toxicity
Liver - enzymology
Male
Rats
Rats, Sprague-Dawley
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
title Induction of liver cytochrome P450 1A2 expression by flutamide in rats
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