Induction of liver cytochrome P450 1A2 expression by flutamide in rats
To investigate the modulation of liver cytochrome P450 1A2 (CYP1A2) expression by giving flutamide to adult rats. Rats were given 50, 100, and 200 mg/kg po of flutamide for 2 weeks. Liver CYP1A2 mRNA was measured using reverse transcription-polymerase chain reaction. CYP1A2 protein was detected usin...
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Veröffentlicht in: | Acta pharmacologica Sinica 2005-11, Vol.26 (11), p.1382-1386 |
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creator | Wang, Hai-xue Liu, Xiao Xu, Chang-jiang Ma, Xiao-chao Long, Jian-er Li, Duan |
description | To investigate the modulation of liver cytochrome P450 1A2 (CYP1A2) expression by giving flutamide to adult rats.
Rats were given 50, 100, and 200 mg/kg po of flutamide for 2 weeks. Liver CYP1A2 mRNA was measured using reverse transcription-polymerase chain reaction. CYP1A2 protein was detected using immunoblotting. CYP1A2 activity was assayed using high performance liquid chromatography, with caffeine as the CYP1A2 substrate.
CYP1A2 mRNA levels after flutamide treatment at 100 mg/kg and 200 mg/kg were, respectively, 1.86 and 3.11-fold higher than those of the control. Correspondingly, CYP1A2 protein increased 1.78 and 2.89-fold and CYP1A2 activity increased approximately 1.65 and 2.83-fold, respectively, relative to controls. Flutamide treatment at 50 mg/kg had no significant effect on CYP1A2 mRNA, protein, or enzyme activity.
Giving rats flutamide induced liver CYP1A2 expression in a dose-dependent manner. |
doi_str_mv | 10.1111/j.1745-7254.2005.00211.x |
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Rats were given 50, 100, and 200 mg/kg po of flutamide for 2 weeks. Liver CYP1A2 mRNA was measured using reverse transcription-polymerase chain reaction. CYP1A2 protein was detected using immunoblotting. CYP1A2 activity was assayed using high performance liquid chromatography, with caffeine as the CYP1A2 substrate.
CYP1A2 mRNA levels after flutamide treatment at 100 mg/kg and 200 mg/kg were, respectively, 1.86 and 3.11-fold higher than those of the control. Correspondingly, CYP1A2 protein increased 1.78 and 2.89-fold and CYP1A2 activity increased approximately 1.65 and 2.83-fold, respectively, relative to controls. Flutamide treatment at 50 mg/kg had no significant effect on CYP1A2 mRNA, protein, or enzyme activity.
Giving rats flutamide induced liver CYP1A2 expression in a dose-dependent manner.</description><identifier>ISSN: 1671-4083</identifier><identifier>EISSN: 1745-7254</identifier><identifier>DOI: 10.1111/j.1745-7254.2005.00211.x</identifier><identifier>PMID: 16225762</identifier><language>eng</language><publisher>United States: Nature Publishing Group</publisher><subject>Androgen Antagonists - toxicity ; Animals ; Cytochrome P-450 CYP1A2 - biosynthesis ; Cytochrome P-450 CYP1A2 - genetics ; Dose-Response Relationship, Drug ; Flutamide - administration & dosage ; Flutamide - toxicity ; Liver - enzymology ; Male ; Rats ; Rats, Sprague-Dawley ; RNA, Messenger - biosynthesis ; RNA, Messenger - genetics</subject><ispartof>Acta pharmacologica Sinica, 2005-11, Vol.26 (11), p.1382-1386</ispartof><rights>Copyright Nature Publishing Group Nov 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16225762$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Hai-xue</creatorcontrib><creatorcontrib>Liu, Xiao</creatorcontrib><creatorcontrib>Xu, Chang-jiang</creatorcontrib><creatorcontrib>Ma, Xiao-chao</creatorcontrib><creatorcontrib>Long, Jian-er</creatorcontrib><creatorcontrib>Li, Duan</creatorcontrib><title>Induction of liver cytochrome P450 1A2 expression by flutamide in rats</title><title>Acta pharmacologica Sinica</title><addtitle>Acta Pharmacol Sin</addtitle><description>To investigate the modulation of liver cytochrome P450 1A2 (CYP1A2) expression by giving flutamide to adult rats.
Rats were given 50, 100, and 200 mg/kg po of flutamide for 2 weeks. Liver CYP1A2 mRNA was measured using reverse transcription-polymerase chain reaction. CYP1A2 protein was detected using immunoblotting. CYP1A2 activity was assayed using high performance liquid chromatography, with caffeine as the CYP1A2 substrate.
CYP1A2 mRNA levels after flutamide treatment at 100 mg/kg and 200 mg/kg were, respectively, 1.86 and 3.11-fold higher than those of the control. Correspondingly, CYP1A2 protein increased 1.78 and 2.89-fold and CYP1A2 activity increased approximately 1.65 and 2.83-fold, respectively, relative to controls. Flutamide treatment at 50 mg/kg had no significant effect on CYP1A2 mRNA, protein, or enzyme activity.
Giving rats flutamide induced liver CYP1A2 expression in a dose-dependent manner.</description><subject>Androgen Antagonists - toxicity</subject><subject>Animals</subject><subject>Cytochrome P-450 CYP1A2 - biosynthesis</subject><subject>Cytochrome P-450 CYP1A2 - genetics</subject><subject>Dose-Response Relationship, Drug</subject><subject>Flutamide - administration & dosage</subject><subject>Flutamide - toxicity</subject><subject>Liver - enzymology</subject><subject>Male</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>RNA, Messenger - biosynthesis</subject><subject>RNA, Messenger - genetics</subject><issn>1671-4083</issn><issn>1745-7254</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNo90EtLw0AQAOBFFFurf0EW74mzs6_kWIrVQkEPeg6b7AZTmoe7iTT_3pTWzmUG5mOGGUIog5hN8byLmRYy0ihFjAAyBkDG4sMVmV8a11OtNIsEJHxG7kLYAXDkLL0lM6YQpVY4J-tNY4eir9qGtiXdV7_O02Ls2-Lbt7WjH0ICZUuk7tB5F8LR5SMt90Nv6so6WjXUmz7ck5vS7IN7OOcF-Vq_fK7eou3762a13EYNKtZHiSxTxpzmuSowxVKC1ZDnHIQxSVIYya11IkdZGg1C2ERNJxhphM2LUknDF-TpNLfz7c_gQp_t2sE308oMGQeVpIgTejyjIa-dzTpf1caP2f_VE6An0Jh-8O4CTBeO30RA_gf2ZmSb</recordid><startdate>200511</startdate><enddate>200511</enddate><creator>Wang, Hai-xue</creator><creator>Liu, Xiao</creator><creator>Xu, Chang-jiang</creator><creator>Ma, Xiao-chao</creator><creator>Long, Jian-er</creator><creator>Li, Duan</creator><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TK</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>200511</creationdate><title>Induction of liver cytochrome P450 1A2 expression by flutamide in rats</title><author>Wang, Hai-xue ; 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Rats were given 50, 100, and 200 mg/kg po of flutamide for 2 weeks. Liver CYP1A2 mRNA was measured using reverse transcription-polymerase chain reaction. CYP1A2 protein was detected using immunoblotting. CYP1A2 activity was assayed using high performance liquid chromatography, with caffeine as the CYP1A2 substrate.
CYP1A2 mRNA levels after flutamide treatment at 100 mg/kg and 200 mg/kg were, respectively, 1.86 and 3.11-fold higher than those of the control. Correspondingly, CYP1A2 protein increased 1.78 and 2.89-fold and CYP1A2 activity increased approximately 1.65 and 2.83-fold, respectively, relative to controls. Flutamide treatment at 50 mg/kg had no significant effect on CYP1A2 mRNA, protein, or enzyme activity.
Giving rats flutamide induced liver CYP1A2 expression in a dose-dependent manner.</abstract><cop>United States</cop><pub>Nature Publishing Group</pub><pmid>16225762</pmid><doi>10.1111/j.1745-7254.2005.00211.x</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Androgen Antagonists - toxicity Animals Cytochrome P-450 CYP1A2 - biosynthesis Cytochrome P-450 CYP1A2 - genetics Dose-Response Relationship, Drug Flutamide - administration & dosage Flutamide - toxicity Liver - enzymology Male Rats Rats, Sprague-Dawley RNA, Messenger - biosynthesis RNA, Messenger - genetics |
title | Induction of liver cytochrome P450 1A2 expression by flutamide in rats |
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