Fabrication and characterization of hollow needle array for electrokinetic intracellular delivery of biomolecules

Delivering molecules into a single cell is a key technology for differentiation of cells and analyze cell functions. This study aims to develop a method for massive intracellular delivery of biomolecules in a precise manner using electrokinetic forces. We fabricated an array of hollow needles by MEM...

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Hauptverfasser:	Nagai Moeto, Miyamoto Tokuma, Kawashima Takahiro, Shibata Takayuki
Format:	Tagungsbericht
Sprache:	eng
Schlagworte:	Arrays Biomolecules Differentiation (biology) Dimensional changes Ejection Electrodes Electrokinetics Fluorescence Fluorescent dyes Needles Polydimethylsiloxane Silicone resins Substrates
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creator	Nagai Moeto Miyamoto Tokuma Kawashima Takahiro Shibata Takayuki
description	Delivering molecules into a single cell is a key technology for differentiation of cells and analyze cell functions. This study aims to develop a method for massive intracellular delivery of biomolecules in a precise manner using electrokinetic forces. We fabricated an array of hollow needles by MEMS technologies to establish a high-throughput cellular delivery method. Dimensional change of the fabricated needle array was investigated. A method to integrate a needle array, PDMS flow channels, and electrodes was developed. We applied a voltage between electrodes placed inside and outside the needle substrate and observed electrokinetic ejection of fluorescent dyes from the fabricated hollow needle array.
doi_str_mv	10.1063/1.4866628
format	Conference Proceeding
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language	eng
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source	AIP Journals Complete
subjects	Arrays Biomolecules Differentiation (biology) Dimensional changes Ejection Electrodes Electrokinetics Fluorescence Fluorescent dyes Needles Polydimethylsiloxane Silicone resins Substrates
title	Fabrication and characterization of hollow needle array for electrokinetic intracellular delivery of biomolecules
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