Effects of methionine-induced hyperhomocysteinemia on endothelium-dependent vasodilation and oxidative status in healthy adults
Homocysteine-mediated endothelial dysfunction has been proposed to occur via oxidative stress mechanisms in humans. However, there is controversy regarding the effects of homocysteine on endothelial function and oxidative status, which may in part result from age discrepancy across the studies. The...
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| Veröffentlicht in: | Circulation (New York, N.Y.) N.Y.), 2000-02, Vol.101 (5), p.485-490 |
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| creator | CHAO, C.-L KUO, T.-L LEE, Y.-T |
| description | Homocysteine-mediated endothelial dysfunction has been proposed to occur via oxidative stress mechanisms in humans. However, there is controversy regarding the effects of homocysteine on endothelial function and oxidative status, which may in part result from age discrepancy across the studies. The present study was designed to investigate the aging effect on the relationship between endothelium-dependent vasodilation and oxidative status in methionine-induced hyperhomocysteinemia.
Plasma homocysteine, phosphatidylcholine hydroperoxide (PCOOH), P-selectin levels, and brachial artery flow-mediated vasodilation were measured at baseline and 4 hours after an oral methionine load (0.1 g/kg) in 15 younger (21 to 40 years) and 15 older (55 to 70 years) healthy adults. Homocysteine increased from 7.3+/-1.3 micromol/L at baseline to 22.7+/-5.2 micromol/L at 4 hours in younger (P |
| doi_str_mv | 10.1161/01.cir.101.5.485 |
| format | Article |
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Plasma homocysteine, phosphatidylcholine hydroperoxide (PCOOH), P-selectin levels, and brachial artery flow-mediated vasodilation were measured at baseline and 4 hours after an oral methionine load (0.1 g/kg) in 15 younger (21 to 40 years) and 15 older (55 to 70 years) healthy adults. Homocysteine increased from 7.3+/-1.3 micromol/L at baseline to 22.7+/-5.2 micromol/L at 4 hours in younger (P<0.001) and from 7. 4+/-1.4 to 24.3+/-4.5 micromol/L in older adults (P<0.001). PCOOH levels were not significantly different between baseline and 4 hours in both groups (P=0.10 in young; P=0.14 in old). P-selectin, which is expected to increase during oxidative stress, was not changed in older (P=0.08) but decreased in younger adults (P=0.037) at 4 hours. Flow-mediated vasodilation was preserved from 13.1+/-2.1% at baseline to 13.5+/-2.8% at 4 hours in younger (P=0.49) and decreased from 12.8+/-2.4% to 8.5+/-2.8% in older adults (P<0.001).
The present study demonstrates that endothelial dysfunction caused by methionine-induced hyperhomocysteinemia is age-related and is mediated through impaired nitric oxide activity without change of oxidative status. Our data do not support previous hypotheses that endothelial damage by homocysteine is via oxidative stress mechanism in humans.</description><identifier>ISSN: 0009-7322</identifier><identifier>EISSN: 1524-4539</identifier><identifier>DOI: 10.1161/01.cir.101.5.485</identifier><identifier>PMID: 10662744</identifier><identifier>CODEN: CIRCAZ</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott Williams & Wilkins</publisher><subject>Adult ; Aged ; Aging ; Atherosclerosis (general aspects, experimental research) ; Biological and medical sciences ; Blood and lymphatic vessels ; Cardiology. Vascular system ; Endothelium, Vascular - physiopathology ; Female ; Homocysteine - metabolism ; Humans ; Hyperhomocysteinemia - chemically induced ; Hyperhomocysteinemia - metabolism ; Hyperhomocysteinemia - physiopathology ; Male ; Medical sciences ; Methionine ; Middle Aged ; Oxidation-Reduction ; Prospective Studies ; Vasodilation</subject><ispartof>Circulation (New York, N.Y.), 2000-02, Vol.101 (5), p.485-490</ispartof><rights>2000 INIST-CNRS</rights><rights>Copyright American Heart Association, Inc. Feb 8, 2000</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c547t-2be204425baf645a75ab41c55c25d686700c99f525c468d024c03ff717e59e0d3</citedby><cites>FETCH-LOGICAL-c547t-2be204425baf645a75ab41c55c25d686700c99f525c468d024c03ff717e59e0d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3673,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1263358$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10662744$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>CHAO, C.-L</creatorcontrib><creatorcontrib>KUO, T.-L</creatorcontrib><creatorcontrib>LEE, Y.-T</creatorcontrib><title>Effects of methionine-induced hyperhomocysteinemia on endothelium-dependent vasodilation and oxidative status in healthy adults</title><title>Circulation (New York, N.Y.)</title><addtitle>Circulation</addtitle><description>Homocysteine-mediated endothelial dysfunction has been proposed to occur via oxidative stress mechanisms in humans. However, there is controversy regarding the effects of homocysteine on endothelial function and oxidative status, which may in part result from age discrepancy across the studies. The present study was designed to investigate the aging effect on the relationship between endothelium-dependent vasodilation and oxidative status in methionine-induced hyperhomocysteinemia.
Plasma homocysteine, phosphatidylcholine hydroperoxide (PCOOH), P-selectin levels, and brachial artery flow-mediated vasodilation were measured at baseline and 4 hours after an oral methionine load (0.1 g/kg) in 15 younger (21 to 40 years) and 15 older (55 to 70 years) healthy adults. Homocysteine increased from 7.3+/-1.3 micromol/L at baseline to 22.7+/-5.2 micromol/L at 4 hours in younger (P<0.001) and from 7. 4+/-1.4 to 24.3+/-4.5 micromol/L in older adults (P<0.001). PCOOH levels were not significantly different between baseline and 4 hours in both groups (P=0.10 in young; P=0.14 in old). P-selectin, which is expected to increase during oxidative stress, was not changed in older (P=0.08) but decreased in younger adults (P=0.037) at 4 hours. Flow-mediated vasodilation was preserved from 13.1+/-2.1% at baseline to 13.5+/-2.8% at 4 hours in younger (P=0.49) and decreased from 12.8+/-2.4% to 8.5+/-2.8% in older adults (P<0.001).
The present study demonstrates that endothelial dysfunction caused by methionine-induced hyperhomocysteinemia is age-related and is mediated through impaired nitric oxide activity without change of oxidative status. Our data do not support previous hypotheses that endothelial damage by homocysteine is via oxidative stress mechanism in humans.</description><subject>Adult</subject><subject>Aged</subject><subject>Aging</subject><subject>Atherosclerosis (general aspects, experimental research)</subject><subject>Biological and medical sciences</subject><subject>Blood and lymphatic vessels</subject><subject>Cardiology. Vascular system</subject><subject>Endothelium, Vascular - physiopathology</subject><subject>Female</subject><subject>Homocysteine - metabolism</subject><subject>Humans</subject><subject>Hyperhomocysteinemia - chemically induced</subject><subject>Hyperhomocysteinemia - metabolism</subject><subject>Hyperhomocysteinemia - physiopathology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Methionine</subject><subject>Middle Aged</subject><subject>Oxidation-Reduction</subject><subject>Prospective Studies</subject><subject>Vasodilation</subject><issn>0009-7322</issn><issn>1524-4539</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkM2LFDEUxIMo7uzq3ZME8dpjvjN9lGF1FxYE0XPIJC90lu5kTNKLc9p_3cgM6Kko3q_qQSH0jpItpYp-InTrYtnSrnIrdvIF2lDJxCAkH1-iDSFkHDRn7Apd1_rYreJavkZXlCjFtBAb9HwbArhWcQ54gTbFnGKCISa_OvB4Oh2hTHnJ7lQb9MsSLc4JQ_K5TTDHdRk8HLuF1PCTrdnH2bbegm3yOP-OvrsnwLXZtlYcE57Azm06YevXudU36FWwc4W3F71BP7_c_tjfDQ_fvt7vPz8MTgrdBnYARoRg8mCDEtJqaQ-COikdk17tlCbEjWOQTDqhdp4w4QgPQVMNcgTi-Q36cO49lvxrhdrMY15L6i8No0zpkTPZIXKGXMm1FgjmWOJiy8lQYv4Obgg1-_vv3VIjTR-8R95fetfDAv6_wHnhDny8ALY6O4dik4v1H8cU53LH_wBYmotQ</recordid><startdate>20000208</startdate><enddate>20000208</enddate><creator>CHAO, C.-L</creator><creator>KUO, T.-L</creator><creator>LEE, Y.-T</creator><general>Lippincott Williams & Wilkins</general><general>American Heart Association, Inc</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>U9A</scope></search><sort><creationdate>20000208</creationdate><title>Effects of methionine-induced hyperhomocysteinemia on endothelium-dependent vasodilation and oxidative status in healthy adults</title><author>CHAO, C.-L ; KUO, T.-L ; LEE, Y.-T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c547t-2be204425baf645a75ab41c55c25d686700c99f525c468d024c03ff717e59e0d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aging</topic><topic>Atherosclerosis (general aspects, experimental research)</topic><topic>Biological and medical sciences</topic><topic>Blood and lymphatic vessels</topic><topic>Cardiology. Vascular system</topic><topic>Endothelium, Vascular - physiopathology</topic><topic>Female</topic><topic>Homocysteine - metabolism</topic><topic>Humans</topic><topic>Hyperhomocysteinemia - chemically induced</topic><topic>Hyperhomocysteinemia - metabolism</topic><topic>Hyperhomocysteinemia - physiopathology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Methionine</topic><topic>Middle Aged</topic><topic>Oxidation-Reduction</topic><topic>Prospective Studies</topic><topic>Vasodilation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CHAO, C.-L</creatorcontrib><creatorcontrib>KUO, T.-L</creatorcontrib><creatorcontrib>LEE, Y.-T</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><jtitle>Circulation (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>CHAO, C.-L</au><au>KUO, T.-L</au><au>LEE, Y.-T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of methionine-induced hyperhomocysteinemia on endothelium-dependent vasodilation and oxidative status in healthy adults</atitle><jtitle>Circulation (New York, N.Y.)</jtitle><addtitle>Circulation</addtitle><date>2000-02-08</date><risdate>2000</risdate><volume>101</volume><issue>5</issue><spage>485</spage><epage>490</epage><pages>485-490</pages><issn>0009-7322</issn><eissn>1524-4539</eissn><coden>CIRCAZ</coden><abstract>Homocysteine-mediated endothelial dysfunction has been proposed to occur via oxidative stress mechanisms in humans. However, there is controversy regarding the effects of homocysteine on endothelial function and oxidative status, which may in part result from age discrepancy across the studies. The present study was designed to investigate the aging effect on the relationship between endothelium-dependent vasodilation and oxidative status in methionine-induced hyperhomocysteinemia.
Plasma homocysteine, phosphatidylcholine hydroperoxide (PCOOH), P-selectin levels, and brachial artery flow-mediated vasodilation were measured at baseline and 4 hours after an oral methionine load (0.1 g/kg) in 15 younger (21 to 40 years) and 15 older (55 to 70 years) healthy adults. Homocysteine increased from 7.3+/-1.3 micromol/L at baseline to 22.7+/-5.2 micromol/L at 4 hours in younger (P<0.001) and from 7. 4+/-1.4 to 24.3+/-4.5 micromol/L in older adults (P<0.001). PCOOH levels were not significantly different between baseline and 4 hours in both groups (P=0.10 in young; P=0.14 in old). P-selectin, which is expected to increase during oxidative stress, was not changed in older (P=0.08) but decreased in younger adults (P=0.037) at 4 hours. Flow-mediated vasodilation was preserved from 13.1+/-2.1% at baseline to 13.5+/-2.8% at 4 hours in younger (P=0.49) and decreased from 12.8+/-2.4% to 8.5+/-2.8% in older adults (P<0.001).
The present study demonstrates that endothelial dysfunction caused by methionine-induced hyperhomocysteinemia is age-related and is mediated through impaired nitric oxide activity without change of oxidative status. Our data do not support previous hypotheses that endothelial damage by homocysteine is via oxidative stress mechanism in humans.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott Williams & Wilkins</pub><pmid>10662744</pmid><doi>10.1161/01.cir.101.5.485</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Aged Aging Atherosclerosis (general aspects, experimental research) Biological and medical sciences Blood and lymphatic vessels Cardiology. Vascular system Endothelium, Vascular - physiopathology Female Homocysteine - metabolism Humans Hyperhomocysteinemia - chemically induced Hyperhomocysteinemia - metabolism Hyperhomocysteinemia - physiopathology Male Medical sciences Methionine Middle Aged Oxidation-Reduction Prospective Studies Vasodilation |
| title | Effects of methionine-induced hyperhomocysteinemia on endothelium-dependent vasodilation and oxidative status in healthy adults |
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