Role of Puroindoline A and B Individually and in Combination on Grain Hardness and Starch Association
Endosperm texture in wheat (Triticum aestivum L.) is an important criterion affecting end-product quality. Grain hardness is controlled by the Hardness (Ha) locus which contains the puroindoline a (Pina) and puroindoline b (Pinb) genes. Hard wheats possess mutations in Pina or Pinb while soft wheats...
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| Veröffentlicht in: | Crop science 2007-01, Vol.47 (1), p.67-76 |
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| description | Endosperm texture in wheat (Triticum aestivum L.) is an important criterion affecting end-product quality. Grain hardness is controlled by the Hardness (Ha) locus which contains the puroindoline a (Pina) and puroindoline b (Pinb) genes. Hard wheats possess mutations in Pina or Pinb while soft wheats possess the Pina-D1a and Pinb-D1a alleles. Here, we determined the role of PINA and PINB individually and in combination on grain hardness and association to starch. Pina-D1a or Pinb-D1a overexpressing transgenic lines were crossed to PINA or PINB null hard wheats. The crosses segregated for the transgene and the Ha locus. Random lines from each genotypic class were evaluated in replicated trials over 2 yr. Classes containing only native levels of PINA or PINB were hard textured. Homozygous classes with transgenic addition of PINA to the PINA+/PINB null Ha locus or addition of PINB to the PINA+/PINB null Ha locus were intermediate in texture with grain hardness values of 43.5 and 45.5, respectively. Soft endosperm texture was only obtained when both PINA and PINB were present with the softest grain observed with addition of PINB to the PINA+/PINB null Ha locus. Association of high amounts of either puroindoline to starch required both PINA and PINB. The results indicate that PINA or PINB can act alone leading to intermediate-textured grain or can function together to give a soft grain texture. |
| doi_str_mv | 10.2135/cropsci2006.05.0310 |
| format | Article |
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Grain hardness is controlled by the Hardness (Ha) locus which contains the puroindoline a (Pina) and puroindoline b (Pinb) genes. Hard wheats possess mutations in Pina or Pinb while soft wheats possess the Pina-D1a and Pinb-D1a alleles. Here, we determined the role of PINA and PINB individually and in combination on grain hardness and association to starch. Pina-D1a or Pinb-D1a overexpressing transgenic lines were crossed to PINA or PINB null hard wheats. The crosses segregated for the transgene and the Ha locus. Random lines from each genotypic class were evaluated in replicated trials over 2 yr. Classes containing only native levels of PINA or PINB were hard textured. Homozygous classes with transgenic addition of PINA to the PINA+/PINB null Ha locus or addition of PINB to the PINA+/PINB null Ha locus were intermediate in texture with grain hardness values of 43.5 and 45.5, respectively. Soft endosperm texture was only obtained when both PINA and PINB were present with the softest grain observed with addition of PINB to the PINA+/PINB null Ha locus. Association of high amounts of either puroindoline to starch required both PINA and PINB. The results indicate that PINA or PINB can act alone leading to intermediate-textured grain or can function together to give a soft grain texture.</description><identifier>ISSN: 0011-183X</identifier><identifier>EISSN: 1435-0653</identifier><identifier>DOI: 10.2135/cropsci2006.05.0310</identifier><identifier>CODEN: CRPSAY</identifier><language>eng</language><publisher>Madison, WI: Crop Science Society of America</publisher><subject>Agronomy. Soil science and plant productions ; alleles ; Biological and medical sciences ; chemical constituents of plants ; crop quality ; endosperm ; Fundamental and applied biological sciences. Psychology ; gene expression ; gene segregation ; Genetics and breeding of economic plants ; genotype ; Hardness ; homozygosity ; loci ; Mutation ; Proteins ; puroindolines ; Starch ; Studies ; texture ; transgenic plants ; Triticum aestivum ; Wheat ; wheat protein</subject><ispartof>Crop science, 2007-01, Vol.47 (1), p.67-76</ispartof><rights>Crop Science Society of America</rights><rights>2007 INIST-CNRS</rights><rights>Copyright American Society of Agronomy Jan/Feb 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4250-95252db3a912c36e53ea4a2dd263ea9f70532ac86d86ed33e488bcef57799c313</citedby><cites>FETCH-LOGICAL-c4250-95252db3a912c36e53ea4a2dd263ea9f70532ac86d86ed33e488bcef57799c313</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.2135%2Fcropsci2006.05.0310$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.2135%2Fcropsci2006.05.0310$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18566107$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Wanjugi, H.W</creatorcontrib><creatorcontrib>Hogg, A.C</creatorcontrib><creatorcontrib>Martin, J.M</creatorcontrib><creatorcontrib>Giroux, M.J</creatorcontrib><title>Role of Puroindoline A and B Individually and in Combination on Grain Hardness and Starch Association</title><title>Crop science</title><description>Endosperm texture in wheat (Triticum aestivum L.) is an important criterion affecting end-product quality. Grain hardness is controlled by the Hardness (Ha) locus which contains the puroindoline a (Pina) and puroindoline b (Pinb) genes. Hard wheats possess mutations in Pina or Pinb while soft wheats possess the Pina-D1a and Pinb-D1a alleles. Here, we determined the role of PINA and PINB individually and in combination on grain hardness and association to starch. Pina-D1a or Pinb-D1a overexpressing transgenic lines were crossed to PINA or PINB null hard wheats. The crosses segregated for the transgene and the Ha locus. Random lines from each genotypic class were evaluated in replicated trials over 2 yr. Classes containing only native levels of PINA or PINB were hard textured. Homozygous classes with transgenic addition of PINA to the PINA+/PINB null Ha locus or addition of PINB to the PINA+/PINB null Ha locus were intermediate in texture with grain hardness values of 43.5 and 45.5, respectively. Soft endosperm texture was only obtained when both PINA and PINB were present with the softest grain observed with addition of PINB to the PINA+/PINB null Ha locus. Association of high amounts of either puroindoline to starch required both PINA and PINB. The results indicate that PINA or PINB can act alone leading to intermediate-textured grain or can function together to give a soft grain texture.</description><subject>Agronomy. Soil science and plant productions</subject><subject>alleles</subject><subject>Biological and medical sciences</subject><subject>chemical constituents of plants</subject><subject>crop quality</subject><subject>endosperm</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression</subject><subject>gene segregation</subject><subject>Genetics and breeding of economic plants</subject><subject>genotype</subject><subject>Hardness</subject><subject>homozygosity</subject><subject>loci</subject><subject>Mutation</subject><subject>Proteins</subject><subject>puroindolines</subject><subject>Starch</subject><subject>Studies</subject><subject>texture</subject><subject>transgenic plants</subject><subject>Triticum aestivum</subject><subject>Wheat</subject><subject>wheat protein</subject><issn>0011-183X</issn><issn>1435-0653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNkVuLFDEQhYMoOK7-Ah8Mgo89VpKuvjyOzV4GFnbZccG3UJOkNUtvMiYzyvx7MxfQR6EgxeGrc-CEsfcC5lIo_GxS3GTjJUAzB5yDEvCCzUStsIIG1Us2AxCiEp369pq9yfkJANq-xRlzD3FyPI78fpeiDzZOPji-4BQs_8KXwfpf3u5omvZHyQc-xOe1D7T1MfAy14mKeEPJBpfzEVptKZkffJFzNP4IvmWvRpqye3d-L9jj1eXX4aa6vbteDovbytQSoepRorRrRb2QRjUOlaOapLWyKVs_toBKkuka2zXOKuXqrlsbN2Lb9r1RQl2wjyffTYo_dy5v9VPcpVAitRTFpO_qukDqBJXWck5u1JvknynttQB9qFP_U6cG1Ic6y9WnszVlQ9OYKBif_5522DQC2sJdnbjffnL7_7HWw2qQw8Pd_WpYHnTAc-CHk9FIUdP3VMIeVxKEKl-HAhDVH0lNlM8</recordid><startdate>200701</startdate><enddate>200701</enddate><creator>Wanjugi, H.W</creator><creator>Hogg, A.C</creator><creator>Martin, J.M</creator><creator>Giroux, M.J</creator><general>Crop Science Society of America</general><general>American Society of Agronomy</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>7XB</scope><scope>88I</scope><scope>8AF</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FK</scope><scope>8G5</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>L6V</scope><scope>M0K</scope><scope>M2O</scope><scope>M2P</scope><scope>M7S</scope><scope>MBDVC</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>R05</scope><scope>S0X</scope></search><sort><creationdate>200701</creationdate><title>Role of Puroindoline A and B Individually and in Combination on Grain Hardness and Starch Association</title><author>Wanjugi, H.W ; Hogg, A.C ; Martin, J.M ; Giroux, M.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4250-95252db3a912c36e53ea4a2dd263ea9f70532ac86d86ed33e488bcef57799c313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>alleles</topic><topic>Biological and medical sciences</topic><topic>chemical constituents of plants</topic><topic>crop quality</topic><topic>endosperm</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene expression</topic><topic>gene segregation</topic><topic>Genetics and breeding of economic plants</topic><topic>genotype</topic><topic>Hardness</topic><topic>homozygosity</topic><topic>loci</topic><topic>Mutation</topic><topic>Proteins</topic><topic>puroindolines</topic><topic>Starch</topic><topic>Studies</topic><topic>texture</topic><topic>transgenic plants</topic><topic>Triticum aestivum</topic><topic>Wheat</topic><topic>wheat protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wanjugi, H.W</creatorcontrib><creatorcontrib>Hogg, A.C</creatorcontrib><creatorcontrib>Martin, J.M</creatorcontrib><creatorcontrib>Giroux, M.J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Engineering Collection</collection><collection>Agricultural Science Database</collection><collection>Research Library</collection><collection>Science Database</collection><collection>Engineering Database</collection><collection>Research Library (Corporate)</collection><collection>Environmental Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>University of Michigan</collection><collection>SIRS Editorial</collection><jtitle>Crop science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wanjugi, H.W</au><au>Hogg, A.C</au><au>Martin, J.M</au><au>Giroux, M.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of Puroindoline A and B Individually and in Combination on Grain Hardness and Starch Association</atitle><jtitle>Crop science</jtitle><date>2007-01</date><risdate>2007</risdate><volume>47</volume><issue>1</issue><spage>67</spage><epage>76</epage><pages>67-76</pages><issn>0011-183X</issn><eissn>1435-0653</eissn><coden>CRPSAY</coden><abstract>Endosperm texture in wheat (Triticum aestivum L.) is an important criterion affecting end-product quality. Grain hardness is controlled by the Hardness (Ha) locus which contains the puroindoline a (Pina) and puroindoline b (Pinb) genes. Hard wheats possess mutations in Pina or Pinb while soft wheats possess the Pina-D1a and Pinb-D1a alleles. Here, we determined the role of PINA and PINB individually and in combination on grain hardness and association to starch. Pina-D1a or Pinb-D1a overexpressing transgenic lines were crossed to PINA or PINB null hard wheats. The crosses segregated for the transgene and the Ha locus. Random lines from each genotypic class were evaluated in replicated trials over 2 yr. Classes containing only native levels of PINA or PINB were hard textured. Homozygous classes with transgenic addition of PINA to the PINA+/PINB null Ha locus or addition of PINB to the PINA+/PINB null Ha locus were intermediate in texture with grain hardness values of 43.5 and 45.5, respectively. Soft endosperm texture was only obtained when both PINA and PINB were present with the softest grain observed with addition of PINB to the PINA+/PINB null Ha locus. Association of high amounts of either puroindoline to starch required both PINA and PINB. The results indicate that PINA or PINB can act alone leading to intermediate-textured grain or can function together to give a soft grain texture.</abstract><cop>Madison, WI</cop><pub>Crop Science Society of America</pub><doi>10.2135/cropsci2006.05.0310</doi><tpages>10</tpages></addata></record> |
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| subjects | Agronomy. Soil science and plant productions alleles Biological and medical sciences chemical constituents of plants crop quality endosperm Fundamental and applied biological sciences. Psychology gene expression gene segregation Genetics and breeding of economic plants genotype Hardness homozygosity loci Mutation Proteins puroindolines Starch Studies texture transgenic plants Triticum aestivum Wheat wheat protein |
| title | Role of Puroindoline A and B Individually and in Combination on Grain Hardness and Starch Association |
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