RFLP mapping of resistance to southern root-knot nematode in soybean
In the USA, the southern root-knot nematode [Meloidogyne incognita (Kofoid and White) Chitwood] (Mi) is a serious pathogen of soybean [Glycine max (L) Merrill]. The objectives of this study were the following: (i) to use restriction fragment length polymorphism (RFLP) to identify markers associated...
Gespeichert in:
| Veröffentlicht in: | Crop science 1997-11, Vol.37 (6), p.1903-1909 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1909 |
|---|---|
| container_issue | 6 |
| container_start_page | 1903 |
| container_title | Crop science |
| container_volume | 37 |
| creator | Tamulonis, J.P. (ASGROW Seed Co., Ames, IA.) Luzzi, B.M Hussey, R.S Parrot, W.A Boerma, H.R |
| description | In the USA, the southern root-knot nematode [Meloidogyne incognita (Kofoid and White) Chitwood] (Mi) is a serious pathogen of soybean [Glycine max (L) Merrill]. The objectives of this study were the following: (i) to use restriction fragment length polymorphism (RFLP) to identify markers associated with quantitative trait loci (QTL) conferring Mi resistance, (ii) to estimate the relative contribution to resistance of each QTL, and (iii) to locate each QTL on the molecular map of the soybean genome. PI96354, a plant introduction with a high level of resistance to Mi, was crossed with 'Bossier', a highly susceptible cultivar, and 110 F2 plants were produced and mapped with 121 RFLP markers. A greenhouse screening procedure was used to determine the level of galling for the F2:3 lines. Data were analyzed using single-factor and two-factor analysis of variance and interval mapping. Two QTL were identified which mapped to LG-O and LG-G of the USDA/ARS-Iowa State Univ. (USDA/ARS-ISU) soy-bean RFLP Map. Results showed a major resistance QTL (R2 = 31%) linked to marker G248A-1 on LG-O. The second QTL (R2 = 14%) was located on LG-G in the interval from K493H-1 to Cs008D-1 and was dominant with respect to resistance. The two QTL explained 39% of the variation in Mi galling in a multiple QTL model. Marker G248A-1 may be closely linked to the Rmi1 gene. When galling data was classified into three distinct classes, it mapped distally to G248A-1 on LG-O. Mi resistance also was linked to G248A-1 through simulated marker analysis. The root-knot nematode resistance QTL identified were found in duplicated DNA segments |
| doi_str_mv | 10.2135/cropsci1997.0011183X003700060039x |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_journals_212621405</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A20459562</galeid><sourcerecordid>A20459562</sourcerecordid><originalsourceid>FETCH-LOGICAL-c586X-f514b17feed36d02df9f552bff52103b55c9efcca96f47cde2fde13441fcfdcc3</originalsourceid><addsrcrecordid>eNqVkU9rGzEQxUVpoW7aj1BYSi-FbqI_q93oaLZNGzAkxAn4JmTtjKvUlrbSmtbfvtpsCDn4EnQY0Px4b2YeIV8YPeVMyDMbQ5-sY0o1p5Qyxs7FilLRUErrXNW_V2TGKiFLWkvxmsxGphyht-RdSvcZa1QjZ-TbzcXiutiZvnd-UwQsIiSXBuMtFEMoUtgPvyD6IoYwlL99GAoPOzOEDgrnc_uwBuPfkzdotgk-PNYTcnfx_bb9WS6ufly280Vp5Xm9KlGyas0aBOhE3VHeoUIp-RpRckbFWkqrAK01qsaqsR1w7ICJqmJosbNWnJBPk24fw589pEHfh3302VJzxmvOKioz9HWCNmYL2nkMQzR2Ax6i2QYP6PL3nNNKKlnzjJdH8Pw62Dl7jJ9PfE4gpQio--h2Jh40o3qMRj-LRh-NJmt8ftzDJGu2GPO5XXoS4iwbNeMmdsL-5hEOL_fR7bLl7c3V9bK9HKlj0Cq7fJxc0ARtNjEPcrd8wGWllBD_AQG9tr8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>212621405</pqid></control><display><type>article</type><title>RFLP mapping of resistance to southern root-knot nematode in soybean</title><source>Wiley Online Library Journals Frontfile Complete</source><creator>Tamulonis, J.P. (ASGROW Seed Co., Ames, IA.) ; Luzzi, B.M ; Hussey, R.S ; Parrot, W.A ; Boerma, H.R</creator><creatorcontrib>Tamulonis, J.P. (ASGROW Seed Co., Ames, IA.) ; Luzzi, B.M ; Hussey, R.S ; Parrot, W.A ; Boerma, H.R</creatorcontrib><description>In the USA, the southern root-knot nematode [Meloidogyne incognita (Kofoid and White) Chitwood] (Mi) is a serious pathogen of soybean [Glycine max (L) Merrill]. The objectives of this study were the following: (i) to use restriction fragment length polymorphism (RFLP) to identify markers associated with quantitative trait loci (QTL) conferring Mi resistance, (ii) to estimate the relative contribution to resistance of each QTL, and (iii) to locate each QTL on the molecular map of the soybean genome. PI96354, a plant introduction with a high level of resistance to Mi, was crossed with 'Bossier', a highly susceptible cultivar, and 110 F2 plants were produced and mapped with 121 RFLP markers. A greenhouse screening procedure was used to determine the level of galling for the F2:3 lines. Data were analyzed using single-factor and two-factor analysis of variance and interval mapping. Two QTL were identified which mapped to LG-O and LG-G of the USDA/ARS-Iowa State Univ. (USDA/ARS-ISU) soy-bean RFLP Map. Results showed a major resistance QTL (R2 = 31%) linked to marker G248A-1 on LG-O. The second QTL (R2 = 14%) was located on LG-G in the interval from K493H-1 to Cs008D-1 and was dominant with respect to resistance. The two QTL explained 39% of the variation in Mi galling in a multiple QTL model. Marker G248A-1 may be closely linked to the Rmi1 gene. When galling data was classified into three distinct classes, it mapped distally to G248A-1 on LG-O. Mi resistance also was linked to G248A-1 through simulated marker analysis. The root-knot nematode resistance QTL identified were found in duplicated DNA segments</description><identifier>ISSN: 0011-183X</identifier><identifier>EISSN: 1435-0653</identifier><identifier>DOI: 10.2135/cropsci1997.0011183X003700060039x</identifier><identifier>CODEN: CRPSAY</identifier><language>eng</language><publisher>Madison, WI: Crop Science Society of America</publisher><subject>Agronomy. Soil science and plant productions ; Biological and medical sciences ; Biotechnology ; CARTE GENETIQUE ; CHROMOSOME MAPS ; CROSSBREDS ; Fundamental and applied biological sciences. Psychology ; GENE INTERACTION ; Gene mapping ; Genetic aspects ; GENETIC MAPPING ; GENETIC MAPS ; GENETIC MARKERS ; Genetics ; Genetics and breeding of economic plants ; GENOTIPOS ; GENOTYPE ; GENOTYPES ; GLYCINE MAX ; INTERACCION DE GENES ; INTERACTION GENIQUE ; LOCI ; LOCUS ; MAPAS GENETICOS ; MARCADORES GENETICOS ; MARKER GENES ; MARQUEUR GENETIQUE ; MELOIDOGYNE INCOGNITA ; Molecular biology ; Pest animals ; PEST RESISTANCE ; PRODUCTOS DEL CRUZAMIENTO ; PRODUIT DE CROISEMENT ; QUANTITATIVE TRAITS ; RESISTANCE AUX ORGANISMES NUISIBLES ; RESISTENCIA A LAS PLAGAS ; RFLP ; Root-knot ; Soybean ; Soybeans ; Varietal selection. Specialized plant breeding, plant breeding aims ; Worms</subject><ispartof>Crop science, 1997-11, Vol.37 (6), p.1903-1909</ispartof><rights>Copyright © 1997 Crop Science Society of America</rights><rights>1998 INIST-CNRS</rights><rights>COPYRIGHT 1997 Crop Science Society of America</rights><rights>Copyright American Society of Agronomy Nov 1997</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c586X-f514b17feed36d02df9f552bff52103b55c9efcca96f47cde2fde13441fcfdcc3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.2135%2Fcropsci1997.0011183X003700060039x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.2135%2Fcropsci1997.0011183X003700060039x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2156275$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Tamulonis, J.P. (ASGROW Seed Co., Ames, IA.)</creatorcontrib><creatorcontrib>Luzzi, B.M</creatorcontrib><creatorcontrib>Hussey, R.S</creatorcontrib><creatorcontrib>Parrot, W.A</creatorcontrib><creatorcontrib>Boerma, H.R</creatorcontrib><title>RFLP mapping of resistance to southern root-knot nematode in soybean</title><title>Crop science</title><description>In the USA, the southern root-knot nematode [Meloidogyne incognita (Kofoid and White) Chitwood] (Mi) is a serious pathogen of soybean [Glycine max (L) Merrill]. The objectives of this study were the following: (i) to use restriction fragment length polymorphism (RFLP) to identify markers associated with quantitative trait loci (QTL) conferring Mi resistance, (ii) to estimate the relative contribution to resistance of each QTL, and (iii) to locate each QTL on the molecular map of the soybean genome. PI96354, a plant introduction with a high level of resistance to Mi, was crossed with 'Bossier', a highly susceptible cultivar, and 110 F2 plants were produced and mapped with 121 RFLP markers. A greenhouse screening procedure was used to determine the level of galling for the F2:3 lines. Data were analyzed using single-factor and two-factor analysis of variance and interval mapping. Two QTL were identified which mapped to LG-O and LG-G of the USDA/ARS-Iowa State Univ. (USDA/ARS-ISU) soy-bean RFLP Map. Results showed a major resistance QTL (R2 = 31%) linked to marker G248A-1 on LG-O. The second QTL (R2 = 14%) was located on LG-G in the interval from K493H-1 to Cs008D-1 and was dominant with respect to resistance. The two QTL explained 39% of the variation in Mi galling in a multiple QTL model. Marker G248A-1 may be closely linked to the Rmi1 gene. When galling data was classified into three distinct classes, it mapped distally to G248A-1 on LG-O. Mi resistance also was linked to G248A-1 through simulated marker analysis. The root-knot nematode resistance QTL identified were found in duplicated DNA segments</description><subject>Agronomy. Soil science and plant productions</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>CARTE GENETIQUE</subject><subject>CHROMOSOME MAPS</subject><subject>CROSSBREDS</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE INTERACTION</subject><subject>Gene mapping</subject><subject>Genetic aspects</subject><subject>GENETIC MAPPING</subject><subject>GENETIC MAPS</subject><subject>GENETIC MARKERS</subject><subject>Genetics</subject><subject>Genetics and breeding of economic plants</subject><subject>GENOTIPOS</subject><subject>GENOTYPE</subject><subject>GENOTYPES</subject><subject>GLYCINE MAX</subject><subject>INTERACCION DE GENES</subject><subject>INTERACTION GENIQUE</subject><subject>LOCI</subject><subject>LOCUS</subject><subject>MAPAS GENETICOS</subject><subject>MARCADORES GENETICOS</subject><subject>MARKER GENES</subject><subject>MARQUEUR GENETIQUE</subject><subject>MELOIDOGYNE INCOGNITA</subject><subject>Molecular biology</subject><subject>Pest animals</subject><subject>PEST RESISTANCE</subject><subject>PRODUCTOS DEL CRUZAMIENTO</subject><subject>PRODUIT DE CROISEMENT</subject><subject>QUANTITATIVE TRAITS</subject><subject>RESISTANCE AUX ORGANISMES NUISIBLES</subject><subject>RESISTENCIA A LAS PLAGAS</subject><subject>RFLP</subject><subject>Root-knot</subject><subject>Soybean</subject><subject>Soybeans</subject><subject>Varietal selection. Specialized plant breeding, plant breeding aims</subject><subject>Worms</subject><issn>0011-183X</issn><issn>1435-0653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNqVkU9rGzEQxUVpoW7aj1BYSi-FbqI_q93oaLZNGzAkxAn4JmTtjKvUlrbSmtbfvtpsCDn4EnQY0Px4b2YeIV8YPeVMyDMbQ5-sY0o1p5Qyxs7FilLRUErrXNW_V2TGKiFLWkvxmsxGphyht-RdSvcZa1QjZ-TbzcXiutiZvnd-UwQsIiSXBuMtFEMoUtgPvyD6IoYwlL99GAoPOzOEDgrnc_uwBuPfkzdotgk-PNYTcnfx_bb9WS6ufly280Vp5Xm9KlGyas0aBOhE3VHeoUIp-RpRckbFWkqrAK01qsaqsR1w7ICJqmJosbNWnJBPk24fw589pEHfh3302VJzxmvOKioz9HWCNmYL2nkMQzR2Ax6i2QYP6PL3nNNKKlnzjJdH8Pw62Dl7jJ9PfE4gpQio--h2Jh40o3qMRj-LRh-NJmt8ftzDJGu2GPO5XXoS4iwbNeMmdsL-5hEOL_fR7bLl7c3V9bK9HKlj0Cq7fJxc0ARtNjEPcrd8wGWllBD_AQG9tr8</recordid><startdate>199711</startdate><enddate>199711</enddate><creator>Tamulonis, J.P. (ASGROW Seed Co., Ames, IA.)</creator><creator>Luzzi, B.M</creator><creator>Hussey, R.S</creator><creator>Parrot, W.A</creator><creator>Boerma, H.R</creator><general>Crop Science Society of America</general><general>American Society of Agronomy</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>199711</creationdate><title>RFLP mapping of resistance to southern root-knot nematode in soybean</title><author>Tamulonis, J.P. (ASGROW Seed Co., Ames, IA.) ; Luzzi, B.M ; Hussey, R.S ; Parrot, W.A ; Boerma, H.R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c586X-f514b17feed36d02df9f552bff52103b55c9efcca96f47cde2fde13441fcfdcc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>CARTE GENETIQUE</topic><topic>CHROMOSOME MAPS</topic><topic>CROSSBREDS</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE INTERACTION</topic><topic>Gene mapping</topic><topic>Genetic aspects</topic><topic>GENETIC MAPPING</topic><topic>GENETIC MAPS</topic><topic>GENETIC MARKERS</topic><topic>Genetics</topic><topic>Genetics and breeding of economic plants</topic><topic>GENOTIPOS</topic><topic>GENOTYPE</topic><topic>GENOTYPES</topic><topic>GLYCINE MAX</topic><topic>INTERACCION DE GENES</topic><topic>INTERACTION GENIQUE</topic><topic>LOCI</topic><topic>LOCUS</topic><topic>MAPAS GENETICOS</topic><topic>MARCADORES GENETICOS</topic><topic>MARKER GENES</topic><topic>MARQUEUR GENETIQUE</topic><topic>MELOIDOGYNE INCOGNITA</topic><topic>Molecular biology</topic><topic>Pest animals</topic><topic>PEST RESISTANCE</topic><topic>PRODUCTOS DEL CRUZAMIENTO</topic><topic>PRODUIT DE CROISEMENT</topic><topic>QUANTITATIVE TRAITS</topic><topic>RESISTANCE AUX ORGANISMES NUISIBLES</topic><topic>RESISTENCIA A LAS PLAGAS</topic><topic>RFLP</topic><topic>Root-knot</topic><topic>Soybean</topic><topic>Soybeans</topic><topic>Varietal selection. Specialized plant breeding, plant breeding aims</topic><topic>Worms</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tamulonis, J.P. (ASGROW Seed Co., Ames, IA.)</creatorcontrib><creatorcontrib>Luzzi, B.M</creatorcontrib><creatorcontrib>Hussey, R.S</creatorcontrib><creatorcontrib>Parrot, W.A</creatorcontrib><creatorcontrib>Boerma, H.R</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Crop science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tamulonis, J.P. (ASGROW Seed Co., Ames, IA.)</au><au>Luzzi, B.M</au><au>Hussey, R.S</au><au>Parrot, W.A</au><au>Boerma, H.R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RFLP mapping of resistance to southern root-knot nematode in soybean</atitle><jtitle>Crop science</jtitle><date>1997-11</date><risdate>1997</risdate><volume>37</volume><issue>6</issue><spage>1903</spage><epage>1909</epage><pages>1903-1909</pages><issn>0011-183X</issn><eissn>1435-0653</eissn><coden>CRPSAY</coden><abstract>In the USA, the southern root-knot nematode [Meloidogyne incognita (Kofoid and White) Chitwood] (Mi) is a serious pathogen of soybean [Glycine max (L) Merrill]. The objectives of this study were the following: (i) to use restriction fragment length polymorphism (RFLP) to identify markers associated with quantitative trait loci (QTL) conferring Mi resistance, (ii) to estimate the relative contribution to resistance of each QTL, and (iii) to locate each QTL on the molecular map of the soybean genome. PI96354, a plant introduction with a high level of resistance to Mi, was crossed with 'Bossier', a highly susceptible cultivar, and 110 F2 plants were produced and mapped with 121 RFLP markers. A greenhouse screening procedure was used to determine the level of galling for the F2:3 lines. Data were analyzed using single-factor and two-factor analysis of variance and interval mapping. Two QTL were identified which mapped to LG-O and LG-G of the USDA/ARS-Iowa State Univ. (USDA/ARS-ISU) soy-bean RFLP Map. Results showed a major resistance QTL (R2 = 31%) linked to marker G248A-1 on LG-O. The second QTL (R2 = 14%) was located on LG-G in the interval from K493H-1 to Cs008D-1 and was dominant with respect to resistance. The two QTL explained 39% of the variation in Mi galling in a multiple QTL model. Marker G248A-1 may be closely linked to the Rmi1 gene. When galling data was classified into three distinct classes, it mapped distally to G248A-1 on LG-O. Mi resistance also was linked to G248A-1 through simulated marker analysis. The root-knot nematode resistance QTL identified were found in duplicated DNA segments</abstract><cop>Madison, WI</cop><pub>Crop Science Society of America</pub><doi>10.2135/cropsci1997.0011183X003700060039x</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0011-183X |
| ispartof | Crop science, 1997-11, Vol.37 (6), p.1903-1909 |
| issn | 0011-183X 1435-0653 |
| language | eng |
| recordid | cdi_proquest_journals_212621405 |
| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Agronomy. Soil science and plant productions Biological and medical sciences Biotechnology CARTE GENETIQUE CHROMOSOME MAPS CROSSBREDS Fundamental and applied biological sciences. Psychology GENE INTERACTION Gene mapping Genetic aspects GENETIC MAPPING GENETIC MAPS GENETIC MARKERS Genetics Genetics and breeding of economic plants GENOTIPOS GENOTYPE GENOTYPES GLYCINE MAX INTERACCION DE GENES INTERACTION GENIQUE LOCI LOCUS MAPAS GENETICOS MARCADORES GENETICOS MARKER GENES MARQUEUR GENETIQUE MELOIDOGYNE INCOGNITA Molecular biology Pest animals PEST RESISTANCE PRODUCTOS DEL CRUZAMIENTO PRODUIT DE CROISEMENT QUANTITATIVE TRAITS RESISTANCE AUX ORGANISMES NUISIBLES RESISTENCIA A LAS PLAGAS RFLP Root-knot Soybean Soybeans Varietal selection. Specialized plant breeding, plant breeding aims Worms |
| title | RFLP mapping of resistance to southern root-knot nematode in soybean |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-23T21%3A39%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=RFLP%20mapping%20of%20resistance%20to%20southern%20root-knot%20nematode%20in%20soybean&rft.jtitle=Crop%20science&rft.au=Tamulonis,%20J.P.%20(ASGROW%20Seed%20Co.,%20Ames,%20IA.)&rft.date=1997-11&rft.volume=37&rft.issue=6&rft.spage=1903&rft.epage=1909&rft.pages=1903-1909&rft.issn=0011-183X&rft.eissn=1435-0653&rft.coden=CRPSAY&rft_id=info:doi/10.2135/cropsci1997.0011183X003700060039x&rft_dat=%3Cgale_proqu%3EA20459562%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=212621405&rft_id=info:pmid/&rft_galeid=A20459562&rfr_iscdi=true |