Resistance to alfalfa mosaic virus in transgenic burley tobaccos expressing the AMV coat protein gene
There are no known genetic sources of resistance to alfalfa mosaic virus (AMV) in the genus Nicotiana. In this communication, we describe how we genetically engineered an AMV (strain 425) coat protein (CP) gene into three commercial burley tobacco (N. tabacum L.) genotypes, BY 21, TN 86, and KY 8959...
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| Veröffentlicht in: | Crop science 1998-11, Vol.38 (6), p.1661-1668 |
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| creator | Xu, D. (GenApps, Inc., Lexington, KY.) Collins, G.B Hunt, A.G Nielsen, M.T |
| description | There are no known genetic sources of resistance to alfalfa mosaic virus (AMV) in the genus Nicotiana. In this communication, we describe how we genetically engineered an AMV (strain 425) coat protein (CP) gene into three commercial burley tobacco (N. tabacum L.) genotypes, BY 21, TN 86, and KY 8959, and the evaluation of the transgenic lines in greenhouse and field experiments. In a replicated greenhouse trial, 11 AMV-CP transgenic lines in a BY 21 background were resistant to two AMV isolates, AMV-425 and AMV-KY, but the lines were less resistant to another isolate, AMV-NC. In a 1994 field experiment, we found that nine BY 21 AMV-CP transgenic lines were resistant to AMV-KY when inoculated both before and after transplanting. The level of resistance varied among the nine lines. In 1995, 10 randomly selected AMV-CP lines in each of the three genetic backgrounds were evaluated in a replicated field trial. Mean infection scores of each set of lines were significantly less than those of their non-transgenic counterparts, but there were no significant differences among these three mean infection scores. However, there were significant differences among lines within a genetic background for their response to AMV infection. High sensitivity to AMV infection, found in genotypes TN 86 and KY 8959 that possess an endogenous gene for resistance to potyviruses, was not evident in their AMV-CP transgenic lines. The CP mediated resistance to AMV was effective in reducing viral symptoms in the field and it may provide a valuable source for AMV resistance in tobacco |
| doi_str_mv | 10.2135/cropsci1998.0011183X003800060041x |
| format | Article |
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(GenApps, Inc., Lexington, KY.) ; Collins, G.B ; Hunt, A.G ; Nielsen, M.T</creator><creatorcontrib>Xu, D. (GenApps, Inc., Lexington, KY.) ; Collins, G.B ; Hunt, A.G ; Nielsen, M.T</creatorcontrib><description>There are no known genetic sources of resistance to alfalfa mosaic virus (AMV) in the genus Nicotiana. In this communication, we describe how we genetically engineered an AMV (strain 425) coat protein (CP) gene into three commercial burley tobacco (N. tabacum L.) genotypes, BY 21, TN 86, and KY 8959, and the evaluation of the transgenic lines in greenhouse and field experiments. In a replicated greenhouse trial, 11 AMV-CP transgenic lines in a BY 21 background were resistant to two AMV isolates, AMV-425 and AMV-KY, but the lines were less resistant to another isolate, AMV-NC. In a 1994 field experiment, we found that nine BY 21 AMV-CP transgenic lines were resistant to AMV-KY when inoculated both before and after transplanting. The level of resistance varied among the nine lines. In 1995, 10 randomly selected AMV-CP lines in each of the three genetic backgrounds were evaluated in a replicated field trial. Mean infection scores of each set of lines were significantly less than those of their non-transgenic counterparts, but there were no significant differences among these three mean infection scores. However, there were significant differences among lines within a genetic background for their response to AMV infection. High sensitivity to AMV infection, found in genotypes TN 86 and KY 8959 that possess an endogenous gene for resistance to potyviruses, was not evident in their AMV-CP transgenic lines. The CP mediated resistance to AMV was effective in reducing viral symptoms in the field and it may provide a valuable source for AMV resistance in tobacco</description><identifier>ISSN: 0011-183X</identifier><identifier>EISSN: 1435-0653</identifier><identifier>DOI: 10.2135/cropsci1998.0011183X003800060041x</identifier><identifier>CODEN: CRPSAY</identifier><language>eng</language><publisher>Madison, WI: Crop Science Society of America</publisher><subject>Agronomy. Soil science and plant productions ; Alfalfa ; Biological and medical sciences ; BIOLOGICAL DIFFERENCES ; Biotechnology ; Burley tobacco ; DIFERENCIAS BIOLOGICAS ; DIFFERENCE BIOLOGIQUE ; DISEASE RESISTANCE ; EVALUACION ; EVALUATION ; EXPERIMENTACION EN CAMPO ; EXPERIMENTACION IN VIVO ; EXPERIMENTAL INFECTION ; EXPERIMENTATION AU CHAMP ; EXPERIMENTATION IN VIVO ; EXPRESION GENICA ; EXPRESSION DES GENES ; FIELD EXPERIMENTATION ; Field tests ; Fundamental and applied biological sciences. Psychology ; GENE ; GENE EXPRESSION ; GENES ; Genetic aspects ; Genetic engineering ; Genetic engineering applications ; GENETIC RESISTANCE ; GENETIC RESOURCES ; Genetic technics ; Genetics and breeding of economic plants ; GENOTIPOS ; GENOTYPE ; GENOTYPES ; IN VIVO EXPERIMENTATION ; INFECCION EXPERIMENTAL ; INFECTION EXPERIMENTALE ; Methods. Procedures. Technologies ; MICROBIAL PROTEINS ; NICOTIANA TABACUM ; Pest resistance ; Plant breeding: fundamental aspects and methodology ; Plant pathogens ; PLANT RESOURCES ; PLANT VIRUSES ; PLANTAS TRANSGENICAS ; PLANTE TRANSGENIQUE ; POTYVIRUS ; POTYVIRUSES ; PROTEINAS MICROBIANAS ; PROTEINE MICROBIENNE ; Proteins ; RECURSOS GENETICOS ; RECURSOS VEGETALES ; RESISTANCE AUX MALADIES ; RESISTANCE GENETIQUE ; RESISTENCIA A LA ENFERMEDAD ; RESISTENCIA GENETICA ; RESSOURCE GENETIQUE ; RESSOURCE VEGETALE ; STRAIN DIFFERENCES ; Tobacco ; Transgenic animals and transgenic plants ; TRANSGENIC PLANTS ; Varietal selection. Specialized plant breeding, plant breeding aims ; VIRUS DE LAS PLANTAS ; VIRUS DES VEGETAUX ; Virus diseases of plants ; Viruses</subject><ispartof>Crop science, 1998-11, Vol.38 (6), p.1661-1668</ispartof><rights>Copyright © 1998 Crop Science Society of America</rights><rights>1999 INIST-CNRS</rights><rights>COPYRIGHT 1998 Crop Science Society of America</rights><rights>Copyright American Society of Agronomy Nov 1998</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c520X-839e6f93de1e18a279da3a5269eef1a0f16dd5c7f2ada62a4a436d150a01d6e43</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.2135%2Fcropsci1998.0011183X003800060041x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.2135%2Fcropsci1998.0011183X003800060041x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,782,786,1419,27931,27932,45581,45582</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1632122$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Xu, D. (GenApps, Inc., Lexington, KY.)</creatorcontrib><creatorcontrib>Collins, G.B</creatorcontrib><creatorcontrib>Hunt, A.G</creatorcontrib><creatorcontrib>Nielsen, M.T</creatorcontrib><title>Resistance to alfalfa mosaic virus in transgenic burley tobaccos expressing the AMV coat protein gene</title><title>Crop science</title><description>There are no known genetic sources of resistance to alfalfa mosaic virus (AMV) in the genus Nicotiana. In this communication, we describe how we genetically engineered an AMV (strain 425) coat protein (CP) gene into three commercial burley tobacco (N. tabacum L.) genotypes, BY 21, TN 86, and KY 8959, and the evaluation of the transgenic lines in greenhouse and field experiments. In a replicated greenhouse trial, 11 AMV-CP transgenic lines in a BY 21 background were resistant to two AMV isolates, AMV-425 and AMV-KY, but the lines were less resistant to another isolate, AMV-NC. In a 1994 field experiment, we found that nine BY 21 AMV-CP transgenic lines were resistant to AMV-KY when inoculated both before and after transplanting. The level of resistance varied among the nine lines. In 1995, 10 randomly selected AMV-CP lines in each of the three genetic backgrounds were evaluated in a replicated field trial. Mean infection scores of each set of lines were significantly less than those of their non-transgenic counterparts, but there were no significant differences among these three mean infection scores. However, there were significant differences among lines within a genetic background for their response to AMV infection. High sensitivity to AMV infection, found in genotypes TN 86 and KY 8959 that possess an endogenous gene for resistance to potyviruses, was not evident in their AMV-CP transgenic lines. The CP mediated resistance to AMV was effective in reducing viral symptoms in the field and it may provide a valuable source for AMV resistance in tobacco</description><subject>Agronomy. Soil science and plant productions</subject><subject>Alfalfa</subject><subject>Biological and medical sciences</subject><subject>BIOLOGICAL DIFFERENCES</subject><subject>Biotechnology</subject><subject>Burley tobacco</subject><subject>DIFERENCIAS BIOLOGICAS</subject><subject>DIFFERENCE BIOLOGIQUE</subject><subject>DISEASE RESISTANCE</subject><subject>EVALUACION</subject><subject>EVALUATION</subject><subject>EXPERIMENTACION EN CAMPO</subject><subject>EXPERIMENTACION IN VIVO</subject><subject>EXPERIMENTAL INFECTION</subject><subject>EXPERIMENTATION AU CHAMP</subject><subject>EXPERIMENTATION IN VIVO</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>FIELD EXPERIMENTATION</subject><subject>Field tests</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE</subject><subject>GENE EXPRESSION</subject><subject>GENES</subject><subject>Genetic aspects</subject><subject>Genetic engineering</subject><subject>Genetic engineering applications</subject><subject>GENETIC RESISTANCE</subject><subject>GENETIC RESOURCES</subject><subject>Genetic technics</subject><subject>Genetics and breeding of economic plants</subject><subject>GENOTIPOS</subject><subject>GENOTYPE</subject><subject>GENOTYPES</subject><subject>IN VIVO EXPERIMENTATION</subject><subject>INFECCION EXPERIMENTAL</subject><subject>INFECTION EXPERIMENTALE</subject><subject>Methods. Procedures. Technologies</subject><subject>MICROBIAL PROTEINS</subject><subject>NICOTIANA TABACUM</subject><subject>Pest resistance</subject><subject>Plant breeding: fundamental aspects and methodology</subject><subject>Plant pathogens</subject><subject>PLANT RESOURCES</subject><subject>PLANT VIRUSES</subject><subject>PLANTAS TRANSGENICAS</subject><subject>PLANTE TRANSGENIQUE</subject><subject>POTYVIRUS</subject><subject>POTYVIRUSES</subject><subject>PROTEINAS MICROBIANAS</subject><subject>PROTEINE MICROBIENNE</subject><subject>Proteins</subject><subject>RECURSOS GENETICOS</subject><subject>RECURSOS VEGETALES</subject><subject>RESISTANCE AUX MALADIES</subject><subject>RESISTANCE GENETIQUE</subject><subject>RESISTENCIA A LA ENFERMEDAD</subject><subject>RESISTENCIA GENETICA</subject><subject>RESSOURCE GENETIQUE</subject><subject>RESSOURCE VEGETALE</subject><subject>STRAIN DIFFERENCES</subject><subject>Tobacco</subject><subject>Transgenic animals and transgenic plants</subject><subject>TRANSGENIC PLANTS</subject><subject>Varietal selection. Specialized plant breeding, plant breeding aims</subject><subject>VIRUS DE LAS PLANTAS</subject><subject>VIRUS DES VEGETAUX</subject><subject>Virus diseases of plants</subject><subject>Viruses</subject><issn>0011-183X</issn><issn>1435-0653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNqVkU-LFDEQxYMoOK5-BCGIF8Fe86eT7j4OzaoLKys7rswt1KarxywznTHp0Z1vbzW9oIe5SAKB4lfvVeUx9k6KcyW1-eBT3GcfZNPU50JIKWu9FkLXQggrRCkfnrCFLLUphDX6KVtMTDFBz9mLnO8Jq5rKLBjeYA55hMEjHyOHbT9dvosZgue_QjpkHgY-JhjyBgeq3R3SFo8E34H3MXN82CfMOQwbPv5AvvzynfsII9-nOCK1Uhe-ZM9IN-Orx_eM3X68-NZ-Lq6uP122y6vCGyXWRa0btH2jO5Qoa1BV04EGo2yD2EsQvbRdZ3zVK-jAKiih1LaTRoCQncVSn7E3sy6Z_zxgHt19PKSBLJ2SytS1spag9zO0gS26MPSRtvPTmAm2ccA-UHlpdFlZYSXhxQmcToe74E_xy5mniHJO2Lt9CjtIRyeFm7Jz_2TnTmZHGm8f94DsKRD6fR_yXyGraR1FmJ-x3zTC8f99XLtqVXtz_XXVXk7UKWhNLq9nlx6ig02iQW5XhDdENGWp_wDxQMH3</recordid><startdate>199811</startdate><enddate>199811</enddate><creator>Xu, D. (GenApps, Inc., Lexington, KY.)</creator><creator>Collins, G.B</creator><creator>Hunt, A.G</creator><creator>Nielsen, M.T</creator><general>Crop Science Society of America</general><general>American Society of Agronomy</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>199811</creationdate><title>Resistance to alfalfa mosaic virus in transgenic burley tobaccos expressing the AMV coat protein gene</title><author>Xu, D. (GenApps, Inc., Lexington, KY.) ; Collins, G.B ; Hunt, A.G ; Nielsen, M.T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c520X-839e6f93de1e18a279da3a5269eef1a0f16dd5c7f2ada62a4a436d150a01d6e43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Alfalfa</topic><topic>Biological and medical sciences</topic><topic>BIOLOGICAL DIFFERENCES</topic><topic>Biotechnology</topic><topic>Burley tobacco</topic><topic>DIFERENCIAS BIOLOGICAS</topic><topic>DIFFERENCE BIOLOGIQUE</topic><topic>DISEASE RESISTANCE</topic><topic>EVALUACION</topic><topic>EVALUATION</topic><topic>EXPERIMENTACION EN CAMPO</topic><topic>EXPERIMENTACION IN VIVO</topic><topic>EXPERIMENTAL INFECTION</topic><topic>EXPERIMENTATION AU CHAMP</topic><topic>EXPERIMENTATION IN VIVO</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>FIELD EXPERIMENTATION</topic><topic>Field tests</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE</topic><topic>GENE EXPRESSION</topic><topic>GENES</topic><topic>Genetic aspects</topic><topic>Genetic engineering</topic><topic>Genetic engineering applications</topic><topic>GENETIC RESISTANCE</topic><topic>GENETIC RESOURCES</topic><topic>Genetic technics</topic><topic>Genetics and breeding of economic plants</topic><topic>GENOTIPOS</topic><topic>GENOTYPE</topic><topic>GENOTYPES</topic><topic>IN VIVO EXPERIMENTATION</topic><topic>INFECCION EXPERIMENTAL</topic><topic>INFECTION EXPERIMENTALE</topic><topic>Methods. Procedures. Technologies</topic><topic>MICROBIAL PROTEINS</topic><topic>NICOTIANA TABACUM</topic><topic>Pest resistance</topic><topic>Plant breeding: fundamental aspects and methodology</topic><topic>Plant pathogens</topic><topic>PLANT RESOURCES</topic><topic>PLANT VIRUSES</topic><topic>PLANTAS TRANSGENICAS</topic><topic>PLANTE TRANSGENIQUE</topic><topic>POTYVIRUS</topic><topic>POTYVIRUSES</topic><topic>PROTEINAS MICROBIANAS</topic><topic>PROTEINE MICROBIENNE</topic><topic>Proteins</topic><topic>RECURSOS GENETICOS</topic><topic>RECURSOS VEGETALES</topic><topic>RESISTANCE AUX MALADIES</topic><topic>RESISTANCE GENETIQUE</topic><topic>RESISTENCIA A LA ENFERMEDAD</topic><topic>RESISTENCIA GENETICA</topic><topic>RESSOURCE GENETIQUE</topic><topic>RESSOURCE VEGETALE</topic><topic>STRAIN DIFFERENCES</topic><topic>Tobacco</topic><topic>Transgenic animals and transgenic plants</topic><topic>TRANSGENIC PLANTS</topic><topic>Varietal selection. Specialized plant breeding, plant breeding aims</topic><topic>VIRUS DE LAS PLANTAS</topic><topic>VIRUS DES VEGETAUX</topic><topic>Virus diseases of plants</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xu, D. (GenApps, Inc., Lexington, KY.)</creatorcontrib><creatorcontrib>Collins, G.B</creatorcontrib><creatorcontrib>Hunt, A.G</creatorcontrib><creatorcontrib>Nielsen, M.T</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Crop science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, D. (GenApps, Inc., Lexington, KY.)</au><au>Collins, G.B</au><au>Hunt, A.G</au><au>Nielsen, M.T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Resistance to alfalfa mosaic virus in transgenic burley tobaccos expressing the AMV coat protein gene</atitle><jtitle>Crop science</jtitle><date>1998-11</date><risdate>1998</risdate><volume>38</volume><issue>6</issue><spage>1661</spage><epage>1668</epage><pages>1661-1668</pages><issn>0011-183X</issn><eissn>1435-0653</eissn><coden>CRPSAY</coden><abstract>There are no known genetic sources of resistance to alfalfa mosaic virus (AMV) in the genus Nicotiana. In this communication, we describe how we genetically engineered an AMV (strain 425) coat protein (CP) gene into three commercial burley tobacco (N. tabacum L.) genotypes, BY 21, TN 86, and KY 8959, and the evaluation of the transgenic lines in greenhouse and field experiments. In a replicated greenhouse trial, 11 AMV-CP transgenic lines in a BY 21 background were resistant to two AMV isolates, AMV-425 and AMV-KY, but the lines were less resistant to another isolate, AMV-NC. In a 1994 field experiment, we found that nine BY 21 AMV-CP transgenic lines were resistant to AMV-KY when inoculated both before and after transplanting. The level of resistance varied among the nine lines. In 1995, 10 randomly selected AMV-CP lines in each of the three genetic backgrounds were evaluated in a replicated field trial. Mean infection scores of each set of lines were significantly less than those of their non-transgenic counterparts, but there were no significant differences among these three mean infection scores. However, there were significant differences among lines within a genetic background for their response to AMV infection. High sensitivity to AMV infection, found in genotypes TN 86 and KY 8959 that possess an endogenous gene for resistance to potyviruses, was not evident in their AMV-CP transgenic lines. The CP mediated resistance to AMV was effective in reducing viral symptoms in the field and it may provide a valuable source for AMV resistance in tobacco</abstract><cop>Madison, WI</cop><pub>Crop Science Society of America</pub><doi>10.2135/cropsci1998.0011183X003800060041x</doi><tpages>8</tpages></addata></record> |
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| ispartof | Crop science, 1998-11, Vol.38 (6), p.1661-1668 |
| issn | 0011-183X 1435-0653 |
| language | eng |
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| source | Access via Wiley Online Library |
| subjects | Agronomy. Soil science and plant productions Alfalfa Biological and medical sciences BIOLOGICAL DIFFERENCES Biotechnology Burley tobacco DIFERENCIAS BIOLOGICAS DIFFERENCE BIOLOGIQUE DISEASE RESISTANCE EVALUACION EVALUATION EXPERIMENTACION EN CAMPO EXPERIMENTACION IN VIVO EXPERIMENTAL INFECTION EXPERIMENTATION AU CHAMP EXPERIMENTATION IN VIVO EXPRESION GENICA EXPRESSION DES GENES FIELD EXPERIMENTATION Field tests Fundamental and applied biological sciences. Psychology GENE GENE EXPRESSION GENES Genetic aspects Genetic engineering Genetic engineering applications GENETIC RESISTANCE GENETIC RESOURCES Genetic technics Genetics and breeding of economic plants GENOTIPOS GENOTYPE GENOTYPES IN VIVO EXPERIMENTATION INFECCION EXPERIMENTAL INFECTION EXPERIMENTALE Methods. Procedures. Technologies MICROBIAL PROTEINS NICOTIANA TABACUM Pest resistance Plant breeding: fundamental aspects and methodology Plant pathogens PLANT RESOURCES PLANT VIRUSES PLANTAS TRANSGENICAS PLANTE TRANSGENIQUE POTYVIRUS POTYVIRUSES PROTEINAS MICROBIANAS PROTEINE MICROBIENNE Proteins RECURSOS GENETICOS RECURSOS VEGETALES RESISTANCE AUX MALADIES RESISTANCE GENETIQUE RESISTENCIA A LA ENFERMEDAD RESISTENCIA GENETICA RESSOURCE GENETIQUE RESSOURCE VEGETALE STRAIN DIFFERENCES Tobacco Transgenic animals and transgenic plants TRANSGENIC PLANTS Varietal selection. Specialized plant breeding, plant breeding aims VIRUS DE LAS PLANTAS VIRUS DES VEGETAUX Virus diseases of plants Viruses |
| title | Resistance to alfalfa mosaic virus in transgenic burley tobaccos expressing the AMV coat protein gene |
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