Role of miRNA-210, miRNA-21 and miRNA-126 as diagnostic biomarkers in colorectal carcinoma: impact of HIF-1α-VEGF signaling pathway
Colorectal cancer (CRC) is a major cause of death worldwide. Novel non-invasive, high diagnostic value screening test is urgently needed to improve survival rate, treatment and prognosis. Stable, small, circulating microRNA (miRNA) offers unique opportunities for the early diagnosis of several disea...
Gespeichert in:
| Veröffentlicht in: | Molecular and cellular biochemistry 2019-04, Vol.454 (1-2), p.177-189 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 189 |
|---|---|
| container_issue | 1-2 |
| container_start_page | 177 |
| container_title | Molecular and cellular biochemistry |
| container_volume | 454 |
| creator | Sabry, Dina El-Deek, Sahar E. M. Maher, Moataz El-Baz, Mona A. H. El-Bader, Hala M. Amer, Eman Hassan, Elham A. Fathy, Wael El-Deek, Heba E. M. |
| description | Colorectal cancer (CRC) is a major cause of death worldwide. Novel non-invasive, high diagnostic value screening test is urgently needed to improve survival rate, treatment and prognosis. Stable, small, circulating microRNA (miRNA) offers unique opportunities for the early diagnosis of several diseases. It acts as tumor oncogenes or suppressors and involve in cell death, survival, and metastasis. Communication between miRNA and carcinogenesis is critical but it still not clear and needs further investigation. The aim of our study is to evaluate the role of miR-210, miR-21, miR-126, as non-invasive diagnostic biomarkers for screening, early detection of CRC, studying their correlation with prognostic variables, and clarifying the roles of miRNAs on HIF-1α-VEGF signaling pathway. The expression of miR-210, miR-21 and miR-126 was performed using qRT-PCR in adenocarcinoma (no = 35), adenomas (no = 51), and neoplasm free controls (no = 101). Serum levels of VEGF and HIF-1α was determined by ELISA Kit. The results show that the expression of miR-210, miR-21, VEGF, HIF-1α was significantly up-regulated while that miRNA-126 was down-regulated in both adenocarcinoma and adenomas compared with controls (
p
|
| doi_str_mv | 10.1007/s11010-018-3462-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2124645674</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2124645674</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2871-a6ab8ef4747346e384f1e70957f94be3292f71084376c52f005ab60ecac6f1f63</originalsourceid><addsrcrecordid>eNp1kMFuEzEQhi0EoqHwAL1UlrjWMGN77V1uVdS0lSqQKuBqeR07dbtZB3ujqndeiBfhmXCUUE6cbGu--cfzEXKC8AEB9MeCCAgMsGVCKs7wBZlhowWTHXYvyQwEAGtR6yPyppR7qDAgviZHAkSjGwEz8vM2DZ6mQNfx9vM54whnz1dqx-XhgVxRW-gy2tWYyhQd7WNa2_zgc6FxpC4NKXs32YE6m10ca_ETjeuNddMu_ep6wfD3L_b94nJBS1yNdojjim7sdPdon96SV8EOxb87nMfk2-Li6_yK3Xy5vJ6f3zDHW43MKtu3PkgtdV3Xi1YG9Bq6RodO9l7wjgeN0EqhlWt4AGhsr8A761TAoMQxeb_P3eT0Y-vLZO7TNte_FMORSyUbpWWlcE-5nErJPphNjnXXJ4Ngdt7N3rup3s3Ou8Hac3pI3vZrv3zu-Cu6AnwPlFoaVz7_G_3_1D9GJYpX</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2124645674</pqid></control><display><type>article</type><title>Role of miRNA-210, miRNA-21 and miRNA-126 as diagnostic biomarkers in colorectal carcinoma: impact of HIF-1α-VEGF signaling pathway</title><source>Springer Nature - Complete Springer Journals</source><creator>Sabry, Dina ; El-Deek, Sahar E. M. ; Maher, Moataz ; El-Baz, Mona A. H. ; El-Bader, Hala M. ; Amer, Eman ; Hassan, Elham A. ; Fathy, Wael ; El-Deek, Heba E. M.</creator><creatorcontrib>Sabry, Dina ; El-Deek, Sahar E. M. ; Maher, Moataz ; El-Baz, Mona A. H. ; El-Bader, Hala M. ; Amer, Eman ; Hassan, Elham A. ; Fathy, Wael ; El-Deek, Heba E. M.</creatorcontrib><description>Colorectal cancer (CRC) is a major cause of death worldwide. Novel non-invasive, high diagnostic value screening test is urgently needed to improve survival rate, treatment and prognosis. Stable, small, circulating microRNA (miRNA) offers unique opportunities for the early diagnosis of several diseases. It acts as tumor oncogenes or suppressors and involve in cell death, survival, and metastasis. Communication between miRNA and carcinogenesis is critical but it still not clear and needs further investigation. The aim of our study is to evaluate the role of miR-210, miR-21, miR-126, as non-invasive diagnostic biomarkers for screening, early detection of CRC, studying their correlation with prognostic variables, and clarifying the roles of miRNAs on HIF-1α-VEGF signaling pathway. The expression of miR-210, miR-21 and miR-126 was performed using qRT-PCR in adenocarcinoma (no = 35), adenomas (no = 51), and neoplasm free controls (no = 101). Serum levels of VEGF and HIF-1α was determined by ELISA Kit. The results show that the expression of miR-210, miR-21, VEGF, HIF-1α was significantly up-regulated while that miRNA-126 was down-regulated in both adenocarcinoma and adenomas compared with controls (
p
< 0.001 for each). No significant difference was noted comparing patients with adenocarcinoma and adenomas. The three miRNAs correlated with VEGF, HIF-α. The miR-210 and miR-21 associated with TNM classification and clinical staging of adenocarcinoma (
p
< 0.001) and they show high diagnostic value with sensitivity and specificity 88.6%, 90.1% and 91.4%, 95.0% respectively. Our study revealed that circulating miR-210, miR-21 were up-regulated while miR-126 was down-regulated in CRC and adenomas patients, they all correlated with TNM staging and they had high diagnostic value. HIF-1α VEGF signaling pathways regulated by miRNAs played a role in colon cancer initiation. To the best of our knowledge, this is the first study of this miRNAs panel in CRC in our community. These data suggested that these biomarkers could be a potential novel, non-invasive marker for early diagnosis, screening and predicting prognosis of CRC. Understanding the molecular functions by which miRNAs affect cancer and understanding its roles in modulating the signaling output of VEGF might be fruitful in reducing the incidence and slowing the progression of this dark malignancy.</description><identifier>ISSN: 0300-8177</identifier><identifier>EISSN: 1573-4919</identifier><identifier>DOI: 10.1007/s11010-018-3462-1</identifier><identifier>PMID: 30357530</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Adenocarcinoma ; Biochemistry ; Biomarkers ; Biomedical and Life Sciences ; Cancer ; Carcinogenesis ; Carcinogens ; Cardiology ; Cell death ; Cell interactions ; Cell survival ; Colon ; Colon cancer ; Colorectal cancer ; Colorectal carcinoma ; Correlation ; Diagnosis ; Diagnostic systems ; Enzyme-linked immunosorbent assay ; Life Sciences ; Malignancy ; Medical Biochemistry ; Medical prognosis ; Metastases ; miRNA ; Oncology ; Patients ; Prognosis ; Ribonucleic acid ; RNA ; Screening ; Serum levels ; Signal transduction ; Signaling ; Suppressors ; Survival ; Tumors ; Vascular endothelial growth factor</subject><ispartof>Molecular and cellular biochemistry, 2019-04, Vol.454 (1-2), p.177-189</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2018</rights><rights>Molecular and Cellular Biochemistry is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2871-a6ab8ef4747346e384f1e70957f94be3292f71084376c52f005ab60ecac6f1f63</citedby><cites>FETCH-LOGICAL-c2871-a6ab8ef4747346e384f1e70957f94be3292f71084376c52f005ab60ecac6f1f63</cites><orcidid>0000-0002-6720-3385</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11010-018-3462-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11010-018-3462-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51298</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30357530$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sabry, Dina</creatorcontrib><creatorcontrib>El-Deek, Sahar E. M.</creatorcontrib><creatorcontrib>Maher, Moataz</creatorcontrib><creatorcontrib>El-Baz, Mona A. H.</creatorcontrib><creatorcontrib>El-Bader, Hala M.</creatorcontrib><creatorcontrib>Amer, Eman</creatorcontrib><creatorcontrib>Hassan, Elham A.</creatorcontrib><creatorcontrib>Fathy, Wael</creatorcontrib><creatorcontrib>El-Deek, Heba E. M.</creatorcontrib><title>Role of miRNA-210, miRNA-21 and miRNA-126 as diagnostic biomarkers in colorectal carcinoma: impact of HIF-1α-VEGF signaling pathway</title><title>Molecular and cellular biochemistry</title><addtitle>Mol Cell Biochem</addtitle><addtitle>Mol Cell Biochem</addtitle><description>Colorectal cancer (CRC) is a major cause of death worldwide. Novel non-invasive, high diagnostic value screening test is urgently needed to improve survival rate, treatment and prognosis. Stable, small, circulating microRNA (miRNA) offers unique opportunities for the early diagnosis of several diseases. It acts as tumor oncogenes or suppressors and involve in cell death, survival, and metastasis. Communication between miRNA and carcinogenesis is critical but it still not clear and needs further investigation. The aim of our study is to evaluate the role of miR-210, miR-21, miR-126, as non-invasive diagnostic biomarkers for screening, early detection of CRC, studying their correlation with prognostic variables, and clarifying the roles of miRNAs on HIF-1α-VEGF signaling pathway. The expression of miR-210, miR-21 and miR-126 was performed using qRT-PCR in adenocarcinoma (no = 35), adenomas (no = 51), and neoplasm free controls (no = 101). Serum levels of VEGF and HIF-1α was determined by ELISA Kit. The results show that the expression of miR-210, miR-21, VEGF, HIF-1α was significantly up-regulated while that miRNA-126 was down-regulated in both adenocarcinoma and adenomas compared with controls (
p
< 0.001 for each). No significant difference was noted comparing patients with adenocarcinoma and adenomas. The three miRNAs correlated with VEGF, HIF-α. The miR-210 and miR-21 associated with TNM classification and clinical staging of adenocarcinoma (
p
< 0.001) and they show high diagnostic value with sensitivity and specificity 88.6%, 90.1% and 91.4%, 95.0% respectively. Our study revealed that circulating miR-210, miR-21 were up-regulated while miR-126 was down-regulated in CRC and adenomas patients, they all correlated with TNM staging and they had high diagnostic value. HIF-1α VEGF signaling pathways regulated by miRNAs played a role in colon cancer initiation. To the best of our knowledge, this is the first study of this miRNAs panel in CRC in our community. These data suggested that these biomarkers could be a potential novel, non-invasive marker for early diagnosis, screening and predicting prognosis of CRC. Understanding the molecular functions by which miRNAs affect cancer and understanding its roles in modulating the signaling output of VEGF might be fruitful in reducing the incidence and slowing the progression of this dark malignancy.</description><subject>Adenocarcinoma</subject><subject>Biochemistry</subject><subject>Biomarkers</subject><subject>Biomedical and Life Sciences</subject><subject>Cancer</subject><subject>Carcinogenesis</subject><subject>Carcinogens</subject><subject>Cardiology</subject><subject>Cell death</subject><subject>Cell interactions</subject><subject>Cell survival</subject><subject>Colon</subject><subject>Colon cancer</subject><subject>Colorectal cancer</subject><subject>Colorectal carcinoma</subject><subject>Correlation</subject><subject>Diagnosis</subject><subject>Diagnostic systems</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Life Sciences</subject><subject>Malignancy</subject><subject>Medical Biochemistry</subject><subject>Medical prognosis</subject><subject>Metastases</subject><subject>miRNA</subject><subject>Oncology</subject><subject>Patients</subject><subject>Prognosis</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Screening</subject><subject>Serum levels</subject><subject>Signal transduction</subject><subject>Signaling</subject><subject>Suppressors</subject><subject>Survival</subject><subject>Tumors</subject><subject>Vascular endothelial growth factor</subject><issn>0300-8177</issn><issn>1573-4919</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kMFuEzEQhi0EoqHwAL1UlrjWMGN77V1uVdS0lSqQKuBqeR07dbtZB3ujqndeiBfhmXCUUE6cbGu--cfzEXKC8AEB9MeCCAgMsGVCKs7wBZlhowWTHXYvyQwEAGtR6yPyppR7qDAgviZHAkSjGwEz8vM2DZ6mQNfx9vM54whnz1dqx-XhgVxRW-gy2tWYyhQd7WNa2_zgc6FxpC4NKXs32YE6m10ca_ETjeuNddMu_ep6wfD3L_b94nJBS1yNdojjim7sdPdon96SV8EOxb87nMfk2-Li6_yK3Xy5vJ6f3zDHW43MKtu3PkgtdV3Xi1YG9Bq6RodO9l7wjgeN0EqhlWt4AGhsr8A761TAoMQxeb_P3eT0Y-vLZO7TNte_FMORSyUbpWWlcE-5nErJPphNjnXXJ4Ngdt7N3rup3s3Ou8Hac3pI3vZrv3zu-Cu6AnwPlFoaVz7_G_3_1D9GJYpX</recordid><startdate>201904</startdate><enddate>201904</enddate><creator>Sabry, Dina</creator><creator>El-Deek, Sahar E. M.</creator><creator>Maher, Moataz</creator><creator>El-Baz, Mona A. H.</creator><creator>El-Bader, Hala M.</creator><creator>Amer, Eman</creator><creator>Hassan, Elham A.</creator><creator>Fathy, Wael</creator><creator>El-Deek, Heba E. M.</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><orcidid>https://orcid.org/0000-0002-6720-3385</orcidid></search><sort><creationdate>201904</creationdate><title>Role of miRNA-210, miRNA-21 and miRNA-126 as diagnostic biomarkers in colorectal carcinoma: impact of HIF-1α-VEGF signaling pathway</title><author>Sabry, Dina ; El-Deek, Sahar E. M. ; Maher, Moataz ; El-Baz, Mona A. H. ; El-Bader, Hala M. ; Amer, Eman ; Hassan, Elham A. ; Fathy, Wael ; El-Deek, Heba E. M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2871-a6ab8ef4747346e384f1e70957f94be3292f71084376c52f005ab60ecac6f1f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adenocarcinoma</topic><topic>Biochemistry</topic><topic>Biomarkers</topic><topic>Biomedical and Life Sciences</topic><topic>Cancer</topic><topic>Carcinogenesis</topic><topic>Carcinogens</topic><topic>Cardiology</topic><topic>Cell death</topic><topic>Cell interactions</topic><topic>Cell survival</topic><topic>Colon</topic><topic>Colon cancer</topic><topic>Colorectal cancer</topic><topic>Colorectal carcinoma</topic><topic>Correlation</topic><topic>Diagnosis</topic><topic>Diagnostic systems</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Life Sciences</topic><topic>Malignancy</topic><topic>Medical Biochemistry</topic><topic>Medical prognosis</topic><topic>Metastases</topic><topic>miRNA</topic><topic>Oncology</topic><topic>Patients</topic><topic>Prognosis</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Screening</topic><topic>Serum levels</topic><topic>Signal transduction</topic><topic>Signaling</topic><topic>Suppressors</topic><topic>Survival</topic><topic>Tumors</topic><topic>Vascular endothelial growth factor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sabry, Dina</creatorcontrib><creatorcontrib>El-Deek, Sahar E. M.</creatorcontrib><creatorcontrib>Maher, Moataz</creatorcontrib><creatorcontrib>El-Baz, Mona A. H.</creatorcontrib><creatorcontrib>El-Bader, Hala M.</creatorcontrib><creatorcontrib>Amer, Eman</creatorcontrib><creatorcontrib>Hassan, Elham A.</creatorcontrib><creatorcontrib>Fathy, Wael</creatorcontrib><creatorcontrib>El-Deek, Heba E. M.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><jtitle>Molecular and cellular biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sabry, Dina</au><au>El-Deek, Sahar E. M.</au><au>Maher, Moataz</au><au>El-Baz, Mona A. H.</au><au>El-Bader, Hala M.</au><au>Amer, Eman</au><au>Hassan, Elham A.</au><au>Fathy, Wael</au><au>El-Deek, Heba E. M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of miRNA-210, miRNA-21 and miRNA-126 as diagnostic biomarkers in colorectal carcinoma: impact of HIF-1α-VEGF signaling pathway</atitle><jtitle>Molecular and cellular biochemistry</jtitle><stitle>Mol Cell Biochem</stitle><addtitle>Mol Cell Biochem</addtitle><date>2019-04</date><risdate>2019</risdate><volume>454</volume><issue>1-2</issue><spage>177</spage><epage>189</epage><pages>177-189</pages><issn>0300-8177</issn><eissn>1573-4919</eissn><abstract>Colorectal cancer (CRC) is a major cause of death worldwide. Novel non-invasive, high diagnostic value screening test is urgently needed to improve survival rate, treatment and prognosis. Stable, small, circulating microRNA (miRNA) offers unique opportunities for the early diagnosis of several diseases. It acts as tumor oncogenes or suppressors and involve in cell death, survival, and metastasis. Communication between miRNA and carcinogenesis is critical but it still not clear and needs further investigation. The aim of our study is to evaluate the role of miR-210, miR-21, miR-126, as non-invasive diagnostic biomarkers for screening, early detection of CRC, studying their correlation with prognostic variables, and clarifying the roles of miRNAs on HIF-1α-VEGF signaling pathway. The expression of miR-210, miR-21 and miR-126 was performed using qRT-PCR in adenocarcinoma (no = 35), adenomas (no = 51), and neoplasm free controls (no = 101). Serum levels of VEGF and HIF-1α was determined by ELISA Kit. The results show that the expression of miR-210, miR-21, VEGF, HIF-1α was significantly up-regulated while that miRNA-126 was down-regulated in both adenocarcinoma and adenomas compared with controls (
p
< 0.001 for each). No significant difference was noted comparing patients with adenocarcinoma and adenomas. The three miRNAs correlated with VEGF, HIF-α. The miR-210 and miR-21 associated with TNM classification and clinical staging of adenocarcinoma (
p
< 0.001) and they show high diagnostic value with sensitivity and specificity 88.6%, 90.1% and 91.4%, 95.0% respectively. Our study revealed that circulating miR-210, miR-21 were up-regulated while miR-126 was down-regulated in CRC and adenomas patients, they all correlated with TNM staging and they had high diagnostic value. HIF-1α VEGF signaling pathways regulated by miRNAs played a role in colon cancer initiation. To the best of our knowledge, this is the first study of this miRNAs panel in CRC in our community. These data suggested that these biomarkers could be a potential novel, non-invasive marker for early diagnosis, screening and predicting prognosis of CRC. Understanding the molecular functions by which miRNAs affect cancer and understanding its roles in modulating the signaling output of VEGF might be fruitful in reducing the incidence and slowing the progression of this dark malignancy.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>30357530</pmid><doi>10.1007/s11010-018-3462-1</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-6720-3385</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0300-8177 |
| ispartof | Molecular and cellular biochemistry, 2019-04, Vol.454 (1-2), p.177-189 |
| issn | 0300-8177 1573-4919 |
| language | eng |
| recordid | cdi_proquest_journals_2124645674 |
| source | Springer Nature - Complete Springer Journals |
| subjects | Adenocarcinoma Biochemistry Biomarkers Biomedical and Life Sciences Cancer Carcinogenesis Carcinogens Cardiology Cell death Cell interactions Cell survival Colon Colon cancer Colorectal cancer Colorectal carcinoma Correlation Diagnosis Diagnostic systems Enzyme-linked immunosorbent assay Life Sciences Malignancy Medical Biochemistry Medical prognosis Metastases miRNA Oncology Patients Prognosis Ribonucleic acid RNA Screening Serum levels Signal transduction Signaling Suppressors Survival Tumors Vascular endothelial growth factor |
| title | Role of miRNA-210, miRNA-21 and miRNA-126 as diagnostic biomarkers in colorectal carcinoma: impact of HIF-1α-VEGF signaling pathway |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-22T08%3A50%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Role%20of%20miRNA-210,%20miRNA-21%20and%20miRNA-126%20as%20diagnostic%20biomarkers%20in%20colorectal%20carcinoma:%20impact%20of%20HIF-1%CE%B1-VEGF%20signaling%20pathway&rft.jtitle=Molecular%20and%20cellular%20biochemistry&rft.au=Sabry,%20Dina&rft.date=2019-04&rft.volume=454&rft.issue=1-2&rft.spage=177&rft.epage=189&rft.pages=177-189&rft.issn=0300-8177&rft.eissn=1573-4919&rft_id=info:doi/10.1007/s11010-018-3462-1&rft_dat=%3Cproquest_cross%3E2124645674%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2124645674&rft_id=info:pmid/30357530&rfr_iscdi=true |