Ultrafast chemical-free cell lysis by high speed stream collision induced by surface acoustic waves

This paper reports on a surface acoustic wave (SAW) based cell lysis device on a LiNbO3 substrate by utilizing high speed collision of cells, which are accelerated by acoustic streaming. With varying working powers, cell lysis was achieved within 20 s and more than 95% lysis efficiency. The cell sol...

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Veröffentlicht in:Applied physics letters 2017-04, Vol.110 (14)
Hauptverfasser: Wang, Wenbo, Chen, Yishan, Farooq, Umar, Xuan, Weipeng, Jin, Hao, Dong, Shurong, Luo, Jikui
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Sprache:eng
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Zusammenfassung:This paper reports on a surface acoustic wave (SAW) based cell lysis device on a LiNbO3 substrate by utilizing high speed collision of cells, which are accelerated by acoustic streaming. With varying working powers, cell lysis was achieved within 20 s and more than 95% lysis efficiency. The cell solution volume effect on SAW based lysis was also investigated and proved that it is not the main issue. With the CCK8 based viability test and verification of cell contents by electrophoresis, the efficient lysis results of our devices have been verified.
ISSN:0003-6951
1077-3118
DOI:10.1063/1.4979788