Biomolecular recognition on nanowire surfaces modified by the self-assembled monolayer
Molecular recognition is one of the key factors in designing biosensors due to which nanowires functionalized with molecular recognition have attracted a lot of attention as promising candidates for nanostructures embedded in biosensors. However, the difficulty in real-world applications with analyt...
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| Veröffentlicht in: | Lab on a chip 2018-10, Vol.18 (21), p.3225-3229 |
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| creator | Shimada, Taisuke Yasui, Takao Yokoyama, Asami Goda, Tatsuro Hara, Mitsuo Yanagida, Takeshi Kaji, Noritada Kanai, Masaki Nagashima, Kazuki Miyahara, Yuji Kawai, Tomoji Baba, Yoshinobu |
| description | Molecular recognition is one of the key factors in designing biosensors due to which nanowires functionalized with molecular recognition have attracted a lot of attention as promising candidates for nanostructures embedded in biosensors. However, the difficulty in real-world applications with analytical specificity is that molecular recognition on nanowires mainly depends on antibody modification with multistep modification procedures. Furthermore, the antibody modification suffers from nonspecific adsorption of undesired proteins in body fluid on the nanowires, which causes false responses and lowers sensitivity. Herein, we propose biomolecular recognition using surface-modified nanowires via thiolated 2-methacryloxyethyl phosphorylcholine (MPC-SH). MPC-SH enables self-assembled monolayer (SAM) modification, which contributes to the reduction of nonspecific adsorption of biomolecules onto the nanowires, and the specific capture of a target protein is attained in the presence of calcium ions. Our concept demonstrates the recognition of the biomarker protein on nanowire surfaces modified by MPC-SH SAM with a single step modification procedure. |
| doi_str_mv | 10.1039/c8lc00438b |
| format | Article |
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However, the difficulty in real-world applications with analytical specificity is that molecular recognition on nanowires mainly depends on antibody modification with multistep modification procedures. Furthermore, the antibody modification suffers from nonspecific adsorption of undesired proteins in body fluid on the nanowires, which causes false responses and lowers sensitivity. Herein, we propose biomolecular recognition using surface-modified nanowires via thiolated 2-methacryloxyethyl phosphorylcholine (MPC-SH). MPC-SH enables self-assembled monolayer (SAM) modification, which contributes to the reduction of nonspecific adsorption of biomolecules onto the nanowires, and the specific capture of a target protein is attained in the presence of calcium ions. Our concept demonstrates the recognition of the biomarker protein on nanowire surfaces modified by MPC-SH SAM with a single step modification procedure.</description><identifier>ISSN: 1473-0197</identifier><identifier>EISSN: 1473-0189</identifier><identifier>DOI: 10.1039/c8lc00438b</identifier><identifier>PMID: 30264843</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Adsorption ; Biomarkers ; Biomolecules ; Biosensors ; Body fluids ; Calcium ions ; Gold ; Monolayers ; Nanowires ; Phosphorylcholine ; Proteins ; Recognition ; Self-assembled monolayers ; Self-assembly ; Surface chemistry</subject><ispartof>Lab on a chip, 2018-10, Vol.18 (21), p.3225-3229</ispartof><rights>Copyright Royal Society of Chemistry 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c498t-50c2d82f415ac1c82a6bbc57facf14c13fcf782efe0eaa5fc6f9a96569cd04253</citedby><cites>FETCH-LOGICAL-c498t-50c2d82f415ac1c82a6bbc57facf14c13fcf782efe0eaa5fc6f9a96569cd04253</cites><orcidid>0000-0003-1829-5153 ; 0000-0003-0333-3559 ; 0000-0002-4451-2433 ; 0000-0003-2688-8186</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,27931,27932</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30264843$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shimada, Taisuke</creatorcontrib><creatorcontrib>Yasui, Takao</creatorcontrib><creatorcontrib>Yokoyama, Asami</creatorcontrib><creatorcontrib>Goda, Tatsuro</creatorcontrib><creatorcontrib>Hara, Mitsuo</creatorcontrib><creatorcontrib>Yanagida, Takeshi</creatorcontrib><creatorcontrib>Kaji, Noritada</creatorcontrib><creatorcontrib>Kanai, Masaki</creatorcontrib><creatorcontrib>Nagashima, Kazuki</creatorcontrib><creatorcontrib>Miyahara, Yuji</creatorcontrib><creatorcontrib>Kawai, Tomoji</creatorcontrib><creatorcontrib>Baba, Yoshinobu</creatorcontrib><title>Biomolecular recognition on nanowire surfaces modified by the self-assembled monolayer</title><title>Lab on a chip</title><addtitle>Lab Chip</addtitle><description>Molecular recognition is one of the key factors in designing biosensors due to which nanowires functionalized with molecular recognition have attracted a lot of attention as promising candidates for nanostructures embedded in biosensors. 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Our concept demonstrates the recognition of the biomarker protein on nanowire surfaces modified by MPC-SH SAM with a single step modification procedure.</description><subject>Adsorption</subject><subject>Biomarkers</subject><subject>Biomolecules</subject><subject>Biosensors</subject><subject>Body fluids</subject><subject>Calcium ions</subject><subject>Gold</subject><subject>Monolayers</subject><subject>Nanowires</subject><subject>Phosphorylcholine</subject><subject>Proteins</subject><subject>Recognition</subject><subject>Self-assembled monolayers</subject><subject>Self-assembly</subject><subject>Surface chemistry</subject><issn>1473-0197</issn><issn>1473-0189</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNo9kFtLw0AQhRdRbK2--AMk4JsQ3Wuy-6jBGxR8UV_DZjKrKUm27iZI_73R1sLADGc-zoFDyDmj14wKcwO6BUql0NUBmTOZi5QybQ73t8ln5CTGFaVMyUwfk5mgPJNaijl5v2t851uEsbUhCQj-o2-GxvfJNL3t_XcTMIljcBYwJp2vG9dgnVSbZPicHti61MaIXdVOaud739oNhlNy5Gwb8Wy3F-Tt4f61eEqXL4_Pxe0yBWn0kCoKvNbcSaYsMNDcZlUFKp_CHJPAhAOXa44OKVqrHGTOWJOpzEBNJVdiQS63vuvgv0aMQ7nyY-inyJIzLrlWNBcTdbWlIPgYA7pyHZrOhk3JaPlbYVnoZfFX4d0EX-wsx6rDeo_-dyZ-ACThbZM</recordid><startdate>20181023</startdate><enddate>20181023</enddate><creator>Shimada, Taisuke</creator><creator>Yasui, Takao</creator><creator>Yokoyama, Asami</creator><creator>Goda, Tatsuro</creator><creator>Hara, Mitsuo</creator><creator>Yanagida, Takeshi</creator><creator>Kaji, Noritada</creator><creator>Kanai, Masaki</creator><creator>Nagashima, Kazuki</creator><creator>Miyahara, Yuji</creator><creator>Kawai, Tomoji</creator><creator>Baba, Yoshinobu</creator><general>Royal Society of Chemistry</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SP</scope><scope>7TB</scope><scope>7U5</scope><scope>8FD</scope><scope>FR3</scope><scope>L7M</scope><orcidid>https://orcid.org/0000-0003-1829-5153</orcidid><orcidid>https://orcid.org/0000-0003-0333-3559</orcidid><orcidid>https://orcid.org/0000-0002-4451-2433</orcidid><orcidid>https://orcid.org/0000-0003-2688-8186</orcidid></search><sort><creationdate>20181023</creationdate><title>Biomolecular recognition on nanowire surfaces modified by the self-assembled monolayer</title><author>Shimada, Taisuke ; Yasui, Takao ; Yokoyama, Asami ; Goda, Tatsuro ; Hara, Mitsuo ; Yanagida, Takeshi ; Kaji, Noritada ; Kanai, Masaki ; Nagashima, Kazuki ; Miyahara, Yuji ; Kawai, Tomoji ; Baba, Yoshinobu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c498t-50c2d82f415ac1c82a6bbc57facf14c13fcf782efe0eaa5fc6f9a96569cd04253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Adsorption</topic><topic>Biomarkers</topic><topic>Biomolecules</topic><topic>Biosensors</topic><topic>Body fluids</topic><topic>Calcium ions</topic><topic>Gold</topic><topic>Monolayers</topic><topic>Nanowires</topic><topic>Phosphorylcholine</topic><topic>Proteins</topic><topic>Recognition</topic><topic>Self-assembled monolayers</topic><topic>Self-assembly</topic><topic>Surface chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shimada, Taisuke</creatorcontrib><creatorcontrib>Yasui, Takao</creatorcontrib><creatorcontrib>Yokoyama, Asami</creatorcontrib><creatorcontrib>Goda, Tatsuro</creatorcontrib><creatorcontrib>Hara, Mitsuo</creatorcontrib><creatorcontrib>Yanagida, Takeshi</creatorcontrib><creatorcontrib>Kaji, Noritada</creatorcontrib><creatorcontrib>Kanai, Masaki</creatorcontrib><creatorcontrib>Nagashima, Kazuki</creatorcontrib><creatorcontrib>Miyahara, Yuji</creatorcontrib><creatorcontrib>Kawai, Tomoji</creatorcontrib><creatorcontrib>Baba, Yoshinobu</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Electronics & Communications Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Lab on a chip</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shimada, Taisuke</au><au>Yasui, Takao</au><au>Yokoyama, Asami</au><au>Goda, Tatsuro</au><au>Hara, Mitsuo</au><au>Yanagida, Takeshi</au><au>Kaji, Noritada</au><au>Kanai, Masaki</au><au>Nagashima, Kazuki</au><au>Miyahara, Yuji</au><au>Kawai, Tomoji</au><au>Baba, Yoshinobu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biomolecular recognition on nanowire surfaces modified by the self-assembled monolayer</atitle><jtitle>Lab on a chip</jtitle><addtitle>Lab Chip</addtitle><date>2018-10-23</date><risdate>2018</risdate><volume>18</volume><issue>21</issue><spage>3225</spage><epage>3229</epage><pages>3225-3229</pages><issn>1473-0197</issn><eissn>1473-0189</eissn><abstract>Molecular recognition is one of the key factors in designing biosensors due to which nanowires functionalized with molecular recognition have attracted a lot of attention as promising candidates for nanostructures embedded in biosensors. However, the difficulty in real-world applications with analytical specificity is that molecular recognition on nanowires mainly depends on antibody modification with multistep modification procedures. Furthermore, the antibody modification suffers from nonspecific adsorption of undesired proteins in body fluid on the nanowires, which causes false responses and lowers sensitivity. Herein, we propose biomolecular recognition using surface-modified nanowires via thiolated 2-methacryloxyethyl phosphorylcholine (MPC-SH). MPC-SH enables self-assembled monolayer (SAM) modification, which contributes to the reduction of nonspecific adsorption of biomolecules onto the nanowires, and the specific capture of a target protein is attained in the presence of calcium ions. 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| subjects | Adsorption Biomarkers Biomolecules Biosensors Body fluids Calcium ions Gold Monolayers Nanowires Phosphorylcholine Proteins Recognition Self-assembled monolayers Self-assembly Surface chemistry |
| title | Biomolecular recognition on nanowire surfaces modified by the self-assembled monolayer |
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