Relationship between antioxidant potential and oxidative damage to lipids, proteins and DNA in aged rats
Oxidative stress has been implicated to play a major role in aging and age-related diseases. In the present study, we investigated the effects of aging on the total antioxidant capacity, uric acid, lipid peroxidation, total sulfhydryl group content and damage to DNA in adult (6 months), old (15 mont...
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| Veröffentlicht in: | Physiological research 2007-01, Vol.56 (6), p.757-764 |
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| creator | Sivonová, M Tatarková, Z Duracková, Z Dobrota, D Lehotský, J Matáková, T Kaplán, P |
| description | Oxidative stress has been implicated to play a major role in aging and age-related diseases. In the present study, we investigated the effects of aging on the total antioxidant capacity, uric acid, lipid peroxidation, total sulfhydryl group content and damage to DNA in adult (6 months), old (15 months) and senescent (26 months) male Wistar rats. The antioxidant capacity, determined by phycoerythrin-based TRAP method (total peroxyl radical-trapping potential) was significantly decreased in the plasma and myocardium of old and senescent rats, whereas plasma level of uric acid was elevated in 26-month-old rats. Age-related decline in plasma and heart antioxidant capacity was accompanied by a significant loss in total sulfhydryl group content, increased lipid peroxidation and higher DNA damage in lymphocytes. Correlations between TRAP and oxidative damage to lipids, proteins and DNA suggest that the decline in antioxidant status may play an important role in age-related accumulation of cell damage caused by reactive oxygen species. |
| doi_str_mv | 10.33549/physiolres.931094 |
| format | Article |
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In the present study, we investigated the effects of aging on the total antioxidant capacity, uric acid, lipid peroxidation, total sulfhydryl group content and damage to DNA in adult (6 months), old (15 months) and senescent (26 months) male Wistar rats. The antioxidant capacity, determined by phycoerythrin-based TRAP method (total peroxyl radical-trapping potential) was significantly decreased in the plasma and myocardium of old and senescent rats, whereas plasma level of uric acid was elevated in 26-month-old rats. Age-related decline in plasma and heart antioxidant capacity was accompanied by a significant loss in total sulfhydryl group content, increased lipid peroxidation and higher DNA damage in lymphocytes. Correlations between TRAP and oxidative damage to lipids, proteins and DNA suggest that the decline in antioxidant status may play an important role in age-related accumulation of cell damage caused by reactive oxygen species.</description><identifier>ISSN: 0862-8408</identifier><identifier>EISSN: 1802-9973</identifier><identifier>DOI: 10.33549/physiolres.931094</identifier><identifier>PMID: 17087608</identifier><language>eng</language><publisher>Czech Republic: Institute of Physiology</publisher><subject>Aging ; Aging - metabolism ; Animals ; Antioxidants ; Antioxidants - pharmacology ; Chromans - pharmacology ; Comet Assay ; Diet ; DNA - metabolism ; Heart - drug effects ; Lipid Metabolism - drug effects ; Lymphocytes - drug effects ; Lymphocytes - metabolism ; Male ; Microscopy, Fluorescence ; Myocardium - metabolism ; Nutrition ; Oxidation-Reduction ; Oxidative Stress - drug effects ; Proteins ; Proteins - metabolism ; Rats ; Rats, Wistar ; Rodents ; Sulfhydryl Compounds - metabolism ; Thiobarbituric Acid Reactive Substances - metabolism ; Uric Acid - blood</subject><ispartof>Physiological research, 2007-01, Vol.56 (6), p.757-764</ispartof><rights>Copyright Institute of Physiology 2007</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-a709f1e11da360ce17c5018bcd860f7dbabe87c42f6a543784b62f35f2d4e8f43</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17087608$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sivonová, M</creatorcontrib><creatorcontrib>Tatarková, Z</creatorcontrib><creatorcontrib>Duracková, Z</creatorcontrib><creatorcontrib>Dobrota, D</creatorcontrib><creatorcontrib>Lehotský, J</creatorcontrib><creatorcontrib>Matáková, T</creatorcontrib><creatorcontrib>Kaplán, P</creatorcontrib><title>Relationship between antioxidant potential and oxidative damage to lipids, proteins and DNA in aged rats</title><title>Physiological research</title><addtitle>Physiol Res</addtitle><description>Oxidative stress has been implicated to play a major role in aging and age-related diseases. In the present study, we investigated the effects of aging on the total antioxidant capacity, uric acid, lipid peroxidation, total sulfhydryl group content and damage to DNA in adult (6 months), old (15 months) and senescent (26 months) male Wistar rats. The antioxidant capacity, determined by phycoerythrin-based TRAP method (total peroxyl radical-trapping potential) was significantly decreased in the plasma and myocardium of old and senescent rats, whereas plasma level of uric acid was elevated in 26-month-old rats. Age-related decline in plasma and heart antioxidant capacity was accompanied by a significant loss in total sulfhydryl group content, increased lipid peroxidation and higher DNA damage in lymphocytes. 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In the present study, we investigated the effects of aging on the total antioxidant capacity, uric acid, lipid peroxidation, total sulfhydryl group content and damage to DNA in adult (6 months), old (15 months) and senescent (26 months) male Wistar rats. The antioxidant capacity, determined by phycoerythrin-based TRAP method (total peroxyl radical-trapping potential) was significantly decreased in the plasma and myocardium of old and senescent rats, whereas plasma level of uric acid was elevated in 26-month-old rats. Age-related decline in plasma and heart antioxidant capacity was accompanied by a significant loss in total sulfhydryl group content, increased lipid peroxidation and higher DNA damage in lymphocytes. 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| ispartof | Physiological research, 2007-01, Vol.56 (6), p.757-764 |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Aging Aging - metabolism Animals Antioxidants Antioxidants - pharmacology Chromans - pharmacology Comet Assay Diet DNA - metabolism Heart - drug effects Lipid Metabolism - drug effects Lymphocytes - drug effects Lymphocytes - metabolism Male Microscopy, Fluorescence Myocardium - metabolism Nutrition Oxidation-Reduction Oxidative Stress - drug effects Proteins Proteins - metabolism Rats Rats, Wistar Rodents Sulfhydryl Compounds - metabolism Thiobarbituric Acid Reactive Substances - metabolism Uric Acid - blood |
| title | Relationship between antioxidant potential and oxidative damage to lipids, proteins and DNA in aged rats |
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