Microbial Transformation of Clarias gariepinus Oil by Psuedomonas aeruginosa
Bio transformation of fatty acid from Malaysian catfish, Clarias gariepinus oil was carried out using Pseudomonas aeruginosa. The lipid from freeze-dried catfish flesh was extracted using a modified Folch method with chloroform-methanol mixture as an extracting solvent. The crude lipid substrate was...
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| Veröffentlicht in: | Oriental journal of chemistry 2014-01, Vol.30 (3), p.1147 |
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| creator | Muhammad Nor Omar Shaban, Norhayati Bakar, Latifah Munirah Ahmad Muzammil Zuberdi |
| description | Bio transformation of fatty acid from Malaysian catfish, Clarias gariepinus oil was carried out using Pseudomonas aeruginosa. The lipid from freeze-dried catfish flesh was extracted using a modified Folch method with chloroform-methanol mixture as an extracting solvent. The crude lipid substrate was added to P. aeruginosa culture and incubated for 4 days. After conversion, the products were analyzed by using GC-MS instrument. The result showed that 7,10-dihydroxy-8(E)-octadecenoic acid (DHOD) were abundantly found in the product. The hydroxyl derivative increased while fatty acid contents decreased after bio transformation process. It can be concluded that the bacterial cells had transformed the fatty acids to yield hydroxyl metabolite which can be utilized as |
| doi_str_mv | 10.13005/ojc/300327 |
| format | Article |
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The lipid from freeze-dried catfish flesh was extracted using a modified Folch method with chloroform-methanol mixture as an extracting solvent. The crude lipid substrate was added to P. aeruginosa culture and incubated for 4 days. After conversion, the products were analyzed by using GC-MS instrument. The result showed that 7,10-dihydroxy-8(E)-octadecenoic acid (DHOD) were abundantly found in the product. The hydroxyl derivative increased while fatty acid contents decreased after bio transformation process. It can be concluded that the bacterial cells had transformed the fatty acids to yield hydroxyl metabolite which can be utilized as</description><identifier>ISSN: 0970-020X</identifier><identifier>EISSN: 2231-5039</identifier><identifier>DOI: 10.13005/ojc/300327</identifier><language>eng</language><publisher>Bhopal: Oriental Scientific Publishing Company</publisher><subject>Bacteria ; Catfish ; Chloroform ; Fatty acids ; Fish oils ; Lipids ; Metabolites ; Microorganisms ; Pseudomonas aeruginosa ; Substrates ; Transformations</subject><ispartof>Oriental journal of chemistry, 2014-01, Vol.30 (3), p.1147</ispartof><rights>2014. This work is published under http://creativecommons.org/licenses/by-nc-sa/4.0/ (the “License”). 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| ispartof | Oriental journal of chemistry, 2014-01, Vol.30 (3), p.1147 |
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| subjects | Bacteria Catfish Chloroform Fatty acids Fish oils Lipids Metabolites Microorganisms Pseudomonas aeruginosa Substrates Transformations |
| title | Microbial Transformation of Clarias gariepinus Oil by Psuedomonas aeruginosa |
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