Quantitative Assay for Starch by Colorimetry Using a Desktop Scanner
Solution color change by mixture of iodine with starch is well-known. Here we describe a procedure to produce a standard curve for quantification of starch concentration using colorimetry. The colorimetry is performed by image analysis of a picture of solutions obtained using a desktop scanner. The...
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| Veröffentlicht in: | Journal of chemical education 2004-05, Vol.81 (5), p.702 |
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| creator | Mathews, Kurt R Landmark, James D Stickle, Douglas F |
| description | Solution color change by mixture of iodine with starch is well-known. Here we describe a procedure to produce a standard curve for quantification of starch concentration using colorimetry. The colorimetry is performed by image analysis of a picture of solutions obtained using a desktop scanner. The analysis is performed using a Visual Basic computer program (COLORS.EXE) that measures red, green, and blue pixel intensities from the image. The computer program is provided in Supplemental Material in JCE Online. The lower limit of detection of the described assay is of the order of 10 mg/dL. The measurement of the starch content in potatoes is given as an example. The experiment can serve as an introduction to quantitative analytical chemistry at the high school level and has the desirable feature of using only common and inexpensive reagents. The procedure is likely amenable to further development for other food science experiments. |
| doi_str_mv | 10.1021/ed081p702 |
| format | Article |
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Here we describe a procedure to produce a standard curve for quantification of starch concentration using colorimetry. The colorimetry is performed by image analysis of a picture of solutions obtained using a desktop scanner. The analysis is performed using a Visual Basic computer program (COLORS.EXE) that measures red, green, and blue pixel intensities from the image. The computer program is provided in Supplemental Material in JCE Online. The lower limit of detection of the described assay is of the order of 10 mg/dL. The measurement of the starch content in potatoes is given as an example. The experiment can serve as an introduction to quantitative analytical chemistry at the high school level and has the desirable feature of using only common and inexpensive reagents. The procedure is likely amenable to further development for other food science experiments.</description><identifier>ISSN: 0021-9584</identifier><identifier>EISSN: 1938-1328</identifier><identifier>DOI: 10.1021/ed081p702</identifier><identifier>CODEN: JCEDA8</identifier><language>eng</language><publisher>Easton: Division of Chemical Education</publisher><subject>Analytical chemistry ; Carbohydrates ; Chemistry ; Color ; Colorimetry ; Computer Peripherals ; Computer Software ; Computer Uses in Education ; Computers ; Data acquisition ; Data Analysis ; Food science ; Image acquisition ; Image analysis ; Interfaces ; Measurement Techniques ; Potatoes ; Science activities ; Science Education ; Secondary school students ; Starch ; Teaching</subject><ispartof>Journal of chemical education, 2004-05, Vol.81 (5), p.702</ispartof><rights>Copyright American Chemical Society May 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a371t-1bc2645ad60402c52b116af31e35c44beb06f5752ae6febaec4959c9866c93583</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ed081p702$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ed081p702$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttp://eric.ed.gov/ERICWebPortal/detail?accno=EJ717048$$DView record in ERIC$$Hfree_for_read</backlink></links><search><creatorcontrib>Mathews, Kurt R</creatorcontrib><creatorcontrib>Landmark, James D</creatorcontrib><creatorcontrib>Stickle, Douglas F</creatorcontrib><title>Quantitative Assay for Starch by Colorimetry Using a Desktop Scanner</title><title>Journal of chemical education</title><addtitle>J. Chem. Educ</addtitle><description>Solution color change by mixture of iodine with starch is well-known. Here we describe a procedure to produce a standard curve for quantification of starch concentration using colorimetry. The colorimetry is performed by image analysis of a picture of solutions obtained using a desktop scanner. The analysis is performed using a Visual Basic computer program (COLORS.EXE) that measures red, green, and blue pixel intensities from the image. The computer program is provided in Supplemental Material in JCE Online. The lower limit of detection of the described assay is of the order of 10 mg/dL. The measurement of the starch content in potatoes is given as an example. The experiment can serve as an introduction to quantitative analytical chemistry at the high school level and has the desirable feature of using only common and inexpensive reagents. The procedure is likely amenable to further development for other food science experiments.</description><subject>Analytical chemistry</subject><subject>Carbohydrates</subject><subject>Chemistry</subject><subject>Color</subject><subject>Colorimetry</subject><subject>Computer Peripherals</subject><subject>Computer Software</subject><subject>Computer Uses in Education</subject><subject>Computers</subject><subject>Data acquisition</subject><subject>Data Analysis</subject><subject>Food science</subject><subject>Image acquisition</subject><subject>Image analysis</subject><subject>Interfaces</subject><subject>Measurement Techniques</subject><subject>Potatoes</subject><subject>Science activities</subject><subject>Science Education</subject><subject>Secondary school students</subject><subject>Starch</subject><subject>Teaching</subject><issn>0021-9584</issn><issn>1938-1328</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNplkDtPwzAUhS0EEqUwsDNYSAwMAV8_Enus2vJSJYRK58hxHUgpcbAdpPx7UgWVgekO59N3rg5C50BugFC4tWsiockIPUAjUEwmwKg8RCPSh4kSkh-jkxA2hAAVSo7Q7KXVdayijtW3xZMQdIdL5_Eyam_ecdHhqds6X33a6Du8ClX9hjWe2fARXYOXRte19afoqNTbYM9-7xit7uav04dk8Xz_OJ0sEs0yiAkUhqZc6HVKOKFG0AIg1SUDy4ThvLAFSUuRCaptWtpCW8OVUEbJNDWKCcnG6HLwNt59tTbEfONaX_eVOQVQhPfiHroeIONdCN6WedO_r32XA8l3G-X7jXr2YmCtr8yemz9lkBG-67saYm3CX9V_zQ92fm1t</recordid><startdate>20040501</startdate><enddate>20040501</enddate><creator>Mathews, Kurt R</creator><creator>Landmark, James D</creator><creator>Stickle, Douglas F</creator><general>Division of Chemical Education</general><general>Journal of Chemical Education, Subscription Department</general><general>American Chemical Society</general><scope>7SW</scope><scope>BJH</scope><scope>BNH</scope><scope>BNI</scope><scope>BNJ</scope><scope>BNO</scope><scope>ERI</scope><scope>PET</scope><scope>REK</scope><scope>WWN</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope></search><sort><creationdate>20040501</creationdate><title>Quantitative Assay for Starch by Colorimetry Using a Desktop Scanner</title><author>Mathews, Kurt R ; Landmark, James D ; Stickle, Douglas F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a371t-1bc2645ad60402c52b116af31e35c44beb06f5752ae6febaec4959c9866c93583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Analytical chemistry</topic><topic>Carbohydrates</topic><topic>Chemistry</topic><topic>Color</topic><topic>Colorimetry</topic><topic>Computer Peripherals</topic><topic>Computer Software</topic><topic>Computer Uses in Education</topic><topic>Computers</topic><topic>Data acquisition</topic><topic>Data Analysis</topic><topic>Food science</topic><topic>Image acquisition</topic><topic>Image analysis</topic><topic>Interfaces</topic><topic>Measurement Techniques</topic><topic>Potatoes</topic><topic>Science activities</topic><topic>Science Education</topic><topic>Secondary school students</topic><topic>Starch</topic><topic>Teaching</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mathews, Kurt R</creatorcontrib><creatorcontrib>Landmark, James D</creatorcontrib><creatorcontrib>Stickle, Douglas F</creatorcontrib><collection>ERIC</collection><collection>ERIC (Ovid)</collection><collection>ERIC</collection><collection>ERIC</collection><collection>ERIC (Legacy Platform)</collection><collection>ERIC( SilverPlatter )</collection><collection>ERIC</collection><collection>ERIC PlusText (Legacy Platform)</collection><collection>Education Resources Information Center (ERIC)</collection><collection>ERIC</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><jtitle>Journal of chemical education</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mathews, Kurt R</au><au>Landmark, James D</au><au>Stickle, Douglas F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><ericid>EJ717048</ericid><atitle>Quantitative Assay for Starch by Colorimetry Using a Desktop Scanner</atitle><jtitle>Journal of chemical education</jtitle><addtitle>J. Chem. Educ</addtitle><date>2004-05-01</date><risdate>2004</risdate><volume>81</volume><issue>5</issue><spage>702</spage><pages>702-</pages><issn>0021-9584</issn><eissn>1938-1328</eissn><coden>JCEDA8</coden><abstract>Solution color change by mixture of iodine with starch is well-known. Here we describe a procedure to produce a standard curve for quantification of starch concentration using colorimetry. The colorimetry is performed by image analysis of a picture of solutions obtained using a desktop scanner. The analysis is performed using a Visual Basic computer program (COLORS.EXE) that measures red, green, and blue pixel intensities from the image. The computer program is provided in Supplemental Material in JCE Online. The lower limit of detection of the described assay is of the order of 10 mg/dL. The measurement of the starch content in potatoes is given as an example. 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| source | ACS Publications |
| subjects | Analytical chemistry Carbohydrates Chemistry Color Colorimetry Computer Peripherals Computer Software Computer Uses in Education Computers Data acquisition Data Analysis Food science Image acquisition Image analysis Interfaces Measurement Techniques Potatoes Science activities Science Education Secondary school students Starch Teaching |
| title | Quantitative Assay for Starch by Colorimetry Using a Desktop Scanner |
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