Ovarian modulators of type 1 11 -hydroxysteroid dehydrogenase (11 HSD) activity and intra-follicular cortisol:cortisone ratios correlate with the clinical outcome of IVF

Ovarian modulators of type 1 11 -hydroxysteroid dehydrogenase (11 HSD) activity and intra-follicular cortisol:cortisone ratios correlate with the clinical outcome of IVF

BACKGROUND: Follicular fluid (FF) contains compounds that can modulate NADP

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Veröffentlicht in:Human reproduction (Oxford) 2003-08, Vol.18 (8), p.1603-1612
1. Verfasser: Thurston, L.M.
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container_title Human reproduction (Oxford)
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creator Thurston, L.M.
description BACKGROUND: Follicular fluid (FF) contains compounds that can modulate NADP
doi_str_mv 10.1093/humrep/deg322
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The objective of this study was to investigate the relationships between levels of the ovarian modulators of type 1 11[beta]HSD, intra-follicular cortisol:cortisone ratios and the clinical outcome of IVF cycles. METHODS: A single random sample of FF was aspirated from each of 132 patients undergoing gonadotrophin-stimulated IVF. Components of FF, resolved using C18 column chromatography, were evaluated for effects on NADP<+<-dependent cortisol oxidation in rat kidney homogenates. Intra- follicular steroid concentrations were measured by radioimmunoassays. Clinical pregnancies were confirmed by ultrasonography at 6 weeks post-embryo transfer. RESULTS: Levels of the hydrophilic ovarian 11[beta]HSD stimuli were significantly lower (P < 0.0001) and levels of the hydrophobic ovarian 11[beta]HSD inhibitors were significantly higher (P < 0.002) in conception versus non-conception cycles. Intra-follicular cortisol:cortisone ratios increased with the degree of inhibition of 11[beta]HSD by the hydrophobic FF fractions. FF obtained from conception cycles had significantly higher cortisol:cortisone ratios than samples from non-conception cycles (12.9 + or - 0.3 versus 8.5 + or - 0.2, respectively; P < 0.0001). CONLCUSIONS: Conception by IVF is associated with elevated intra-follicular cortisol:cortisone ratios, which reflect low levels of ovarian stimuli and/or high levels of ovarian inhibitors of type 1 11[beta]HSD.]]></description><identifier>ISSN: 1460-2350</identifier><identifier>ISSN: 0268-1161</identifier><identifier>EISSN: 1460-2350</identifier><identifier>DOI: 10.1093/humrep/deg322</identifier><identifier>CODEN: HRUPF8</identifier><language>eng</language><publisher>Oxford: Oxford Publishing Limited (England)</publisher><ispartof>Human reproduction (Oxford), 2003-08, Vol.18 (8), p.1603-1612</ispartof><rights>Copyright Oxford University Press(England) Aug 2003</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1482-92c870025939d0b278d7b16f3588a18bbc33d5c8ae0407fff470678f23e50a793</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Thurston, L.M.</creatorcontrib><title>Ovarian modulators of type 1 11 -hydroxysteroid dehydrogenase (11 HSD) activity and intra-follicular cortisol:cortisone ratios correlate with the clinical outcome of IVF</title><title>Human reproduction (Oxford)</title><description><![CDATA[BACKGROUND: Follicular fluid (FF) contains compounds that can modulate NADP<+<-dependent oxidation of cortisol by type 1 11[beta]-hydroxysteroid dehydrogenase (11[beta]HSD). The objective of this study was to investigate the relationships between levels of the ovarian modulators of type 1 11[beta]HSD, intra-follicular cortisol:cortisone ratios and the clinical outcome of IVF cycles. METHODS: A single random sample of FF was aspirated from each of 132 patients undergoing gonadotrophin-stimulated IVF. Components of FF, resolved using C18 column chromatography, were evaluated for effects on NADP<+<-dependent cortisol oxidation in rat kidney homogenates. Intra- follicular steroid concentrations were measured by radioimmunoassays. Clinical pregnancies were confirmed by ultrasonography at 6 weeks post-embryo transfer. RESULTS: Levels of the hydrophilic ovarian 11[beta]HSD stimuli were significantly lower (P < 0.0001) and levels of the hydrophobic ovarian 11[beta]HSD inhibitors were significantly higher (P < 0.002) in conception versus non-conception cycles. Intra-follicular cortisol:cortisone ratios increased with the degree of inhibition of 11[beta]HSD by the hydrophobic FF fractions. FF obtained from conception cycles had significantly higher cortisol:cortisone ratios than samples from non-conception cycles (12.9 + or - 0.3 versus 8.5 + or - 0.2, respectively; P < 0.0001). CONLCUSIONS: Conception by IVF is associated with elevated intra-follicular cortisol:cortisone ratios, which reflect low levels of ovarian stimuli and/or high levels of ovarian inhibitors of type 1 11[beta]HSD.]]></description><issn>1460-2350</issn><issn>0268-1161</issn><issn>1460-2350</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNpNkTtPwzAUhSMEEqUwsltMMIT6kYfDhgqllSp14LFGrn3dukrjYjuF_Bt2_gW_jJR2YLpH934650onii4JviW4YINls3awGShYMEqPoh5JMhxTluLjf_o0OvN-hTHJCM960fdsK5wRNVpb1VQiWOeR1Si0G0AEEfLzFS9b5exn6wM4axRS8LdYQC08oOsdMn5-uEFCBrM1oUWiVsjUwYlY26oysrN1SFoXjLfV3UHUgJwIxvrdxUGXDOjDhCUKS0CyMrWRokK2CdKuYffR5G10Hp1oUXm4OMx-9Dp6fBmO4-nsaTK8n8aSJJzGBZU8x5imBSsUntOcq3xOMs1SzgXh87lkTKWSC8AJzrXWSY6znGvKIMUiL1g_utr7bpx9b8CHcmUbV3eRJSWEY04S3EHxHpLOeu9Alxtn1sK1JcHlro5yX0e5r4P9Ane8g7M</recordid><startdate>20030801</startdate><enddate>20030801</enddate><creator>Thurston, L.M.</creator><general>Oxford Publishing Limited (England)</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20030801</creationdate><title>Ovarian modulators of type 1 11 -hydroxysteroid dehydrogenase (11 HSD) activity and intra-follicular cortisol:cortisone ratios correlate with the clinical outcome of IVF</title><author>Thurston, L.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1482-92c870025939d0b278d7b16f3588a18bbc33d5c8ae0407fff470678f23e50a793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thurston, L.M.</creatorcontrib><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Human reproduction (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thurston, L.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ovarian modulators of type 1 11 -hydroxysteroid dehydrogenase (11 HSD) activity and intra-follicular cortisol:cortisone ratios correlate with the clinical outcome of IVF</atitle><jtitle>Human reproduction (Oxford)</jtitle><date>2003-08-01</date><risdate>2003</risdate><volume>18</volume><issue>8</issue><spage>1603</spage><epage>1612</epage><pages>1603-1612</pages><issn>1460-2350</issn><issn>0268-1161</issn><eissn>1460-2350</eissn><coden>HRUPF8</coden><abstract><![CDATA[BACKGROUND: Follicular fluid (FF) contains compounds that can modulate NADP<+<-dependent oxidation of cortisol by type 1 11[beta]-hydroxysteroid dehydrogenase (11[beta]HSD). The objective of this study was to investigate the relationships between levels of the ovarian modulators of type 1 11[beta]HSD, intra-follicular cortisol:cortisone ratios and the clinical outcome of IVF cycles. METHODS: A single random sample of FF was aspirated from each of 132 patients undergoing gonadotrophin-stimulated IVF. Components of FF, resolved using C18 column chromatography, were evaluated for effects on NADP<+<-dependent cortisol oxidation in rat kidney homogenates. Intra- follicular steroid concentrations were measured by radioimmunoassays. Clinical pregnancies were confirmed by ultrasonography at 6 weeks post-embryo transfer. RESULTS: Levels of the hydrophilic ovarian 11[beta]HSD stimuli were significantly lower (P < 0.0001) and levels of the hydrophobic ovarian 11[beta]HSD inhibitors were significantly higher (P < 0.002) in conception versus non-conception cycles. Intra-follicular cortisol:cortisone ratios increased with the degree of inhibition of 11[beta]HSD by the hydrophobic FF fractions. FF obtained from conception cycles had significantly higher cortisol:cortisone ratios than samples from non-conception cycles (12.9 + or - 0.3 versus 8.5 + or - 0.2, respectively; P < 0.0001). CONLCUSIONS: Conception by IVF is associated with elevated intra-follicular cortisol:cortisone ratios, which reflect low levels of ovarian stimuli and/or high levels of ovarian inhibitors of type 1 11[beta]HSD.]]></abstract><cop>Oxford</cop><pub>Oxford Publishing Limited (England)</pub><doi>10.1093/humrep/deg322</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record>
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title Ovarian modulators of type 1 11 -hydroxysteroid dehydrogenase (11 HSD) activity and intra-follicular cortisol:cortisone ratios correlate with the clinical outcome of IVF
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