Determination of Aniline Blue dye by Flow Injection Analysis With Home Made Valve

Flow injection unit with merging zone technique was designed for aniline blue determination spectrophotometrically. This unit consists of a valve was designed for dye injection. The scope of the study is to find the optimum conditions :volume of sample, flow rate of carrier, dead volume, dispersion coefficient and reproducibility. They found 117µl, 4.3 ml/min, zero dead volume, 2.62 and high reproducibility for six times respectively. The range of aniline blue concentration by flow injection analysis was 0. 1 – 8 ppm and detection limit 0.1 with R2= 0.9952 and 0.01 – 16 ppm for spectrophotometric method with R2=0.9947 with detection limit 0.01.