Antioxidant Activity of Alkaloid Compounds from Litsea cubeba Lour

Litsea cubeba (Lour.) heartwood. was extracted by maceration. Ethanol extract was fractionated with liquid-liquid extraction using n-hexane, chloroform at pH 3, 7, 9 and 11 to obtained alkaloid fractions. Isolation of alkaloid compounds were isolated with preparative thin layer chromatography. Antioxidant activity for fractions and isolates were determined with DPPH and ABTS assay. The highest IC50 were given by chloroform fractions at pH 7 and compound III. They IC50 were 23.81 ± 0.01; 3.12 ± 0.02 µg/mL for DPPH assay and 56.43 ± 0.06; 8.62 ± 0.02 µg/mL for ABTS assay. Compound III was showed maximum absorbance at (302.8; 280.8; 206 nm) and wave number (3286.70; 3109.25; 2939.52; 2835.36; 1589.34; 1462.04; 1365.60; 1246.02; 1018.41; 879.54 cm-1) which indicate its an alkaloid compound with hydroxyl, methyl, methylene and methoxy group. The results reveal that alkaloid fractions and compounds of Litsea cubeba heartwood has very strong antioxidant potential.