Role of FGF-2 Transfected Bone Marrow Mesenchymal Stem Cells in Engineered Bone Tissue for Repair of Avascular Necrosis of Femoral Head in Rabbits
Background/Aims: Avascular necrosis of the femoral head (ANFH) is the focus and difficulty of orthopedic diseases. Recently, tissue engineering bone for this disease has shown a good therapeutic effect. The aim of the present study was to investigate the therapeutic effect of basic fibroblast growth...
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| Veröffentlicht in: | Cellular physiology and biochemistry 2018-01, Vol.48 (2), p.773-784 |
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| creator | Zhang, Fei Peng, Wu-xun Wang, Lei Zhang, Jian Dong, Wen-tao Wu, Jian-hua Zhang, Huai Wang, Jian-bo Zhao, Yin |
| description | Background/Aims: Avascular necrosis of the femoral head (ANFH) is the focus and difficulty of orthopedic diseases. Recently, tissue engineering bone for this disease has shown a good therapeutic effect. The aim of the present study was to investigate the therapeutic effect of basic fibroblast growth factor (FGF-2) as cytokines transfected bone marrow mesenchymal stem cells (BMSCs) in constructing tissue-engineered bone for avascular necrosis of the femoral head. Methods: The FGF-2 gene overexpressed lentivirus-transfected rBMSCs with xenogeneic antigen-extracted cancellous bone (XACB) to construct tissue engineered bone, and the model of early avascular necrosis of the femoral head was established by lipopolysaccharide (LPS) combined with hormone. The models were randomly divided into five groups: A (control), B (XACB), C (XACB+rBMSCs), D (XACB+rBMSCs+Lv-GFP), and E (XACB+rBMSCs+Lv-FGF-2/GFP) groups. The therapeutic effect of the tissue engineered bone for the avascular necrosis of the femoral head was evaluated by gross anatomy, X-ray examination, immunohistochemistry and H&E staining. Results: The FGF-2 gene was transfected into rBMSCs (Multiplicity of infection [MOI] = 100) by lentivirus, and its efficiency was above 95%. The rBMSCs were successfully transfected overexpressing FGF-2 by qPCR and western blot. After tissue engineering bone implantation, X-ray examination and gross specimen observation revealed that the repair area in the E group was > 80% at six weeks, the defect was completely repaired at 12 weeks, and osteogenesis was stronger, when compared with the other groups. For the X-ray score, vascular area density and new bone formation area were higher, when compared with the other groups, and the difference was statistically significant (P< 0.05). Conclusion: FGF-2 gene overexpression lentivirus transfection BMSCs combined with XACB to construct tissue engineered bone can effectively promote vascular regeneration, and improve the repair effect of avascular necrosis of the femoral head. |
| doi_str_mv | 10.1159/000491906 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_proquest_journals_2117172346</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_450dd75acc0542d88cf41e7065774e92</doaj_id><sourcerecordid>2073325624</sourcerecordid><originalsourceid>FETCH-LOGICAL-c529t-d3572a442894302ef0d2d98f007ce04502129876e8576a2a859df9eb442bde6f3</originalsourceid><addsrcrecordid>eNptkU1v0zAYgCMEYmNw4I6QJS5wyLAdO46PW7VukzZApZwt135dXJK42Alof4NfjLN2nYQ4-UPP-7xfRfGa4FNCuPyIMWaSSFw_KY4Jo6SUQjRP8x0TXjayEUfFi5Q2OD-FpM-LowpjyitZHxd_FqEFFByaX85LipZR98mBGcCi89ADutUxht_oFhL05vtdp1v0dYAOzaBtE_I9uujXvgeIDwFLn9IIyIWIFrDVPk7ys186mbHVEX0CE0Py6T4ldCFm4RVoO6kWerXyQ3pZPHO6TfBqf54U3-YXy9lVefP58np2dlMaTuVQ2ooLqhmjjWQVpuCwpVY2DmNhADOOKaG59RoaLmpNdcOldRJWOWJloXbVSXG989qgN2obfafjnQraq_uPENdKx8GbFlS2WSu4NgZzRm3TGMcICFxzIRhIml3vd65tDD9HSIPqfDJ5RrqHMCZFsagqymvKMvruH3QTxtjnThUlRBBBK1Zn6sOOmsaVIrhDgQSraenqsPTMvt0bx1UH9kA-bPkx5Q8d1xAPwOzL-U6htnaax5v_UvssfwHeArit</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2117172346</pqid></control><display><type>article</type><title>Role of FGF-2 Transfected Bone Marrow Mesenchymal Stem Cells in Engineered Bone Tissue for Repair of Avascular Necrosis of Femoral Head in Rabbits</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Karger Open Access</source><creator>Zhang, Fei ; Peng, Wu-xun ; Wang, Lei ; Zhang, Jian ; Dong, Wen-tao ; Wu, Jian-hua ; Zhang, Huai ; Wang, Jian-bo ; Zhao, Yin</creator><creatorcontrib>Zhang, Fei ; Peng, Wu-xun ; Wang, Lei ; Zhang, Jian ; Dong, Wen-tao ; Wu, Jian-hua ; Zhang, Huai ; Wang, Jian-bo ; Zhao, Yin</creatorcontrib><description>Background/Aims: Avascular necrosis of the femoral head (ANFH) is the focus and difficulty of orthopedic diseases. Recently, tissue engineering bone for this disease has shown a good therapeutic effect. The aim of the present study was to investigate the therapeutic effect of basic fibroblast growth factor (FGF-2) as cytokines transfected bone marrow mesenchymal stem cells (BMSCs) in constructing tissue-engineered bone for avascular necrosis of the femoral head. Methods: The FGF-2 gene overexpressed lentivirus-transfected rBMSCs with xenogeneic antigen-extracted cancellous bone (XACB) to construct tissue engineered bone, and the model of early avascular necrosis of the femoral head was established by lipopolysaccharide (LPS) combined with hormone. The models were randomly divided into five groups: A (control), B (XACB), C (XACB+rBMSCs), D (XACB+rBMSCs+Lv-GFP), and E (XACB+rBMSCs+Lv-FGF-2/GFP) groups. The therapeutic effect of the tissue engineered bone for the avascular necrosis of the femoral head was evaluated by gross anatomy, X-ray examination, immunohistochemistry and H&E staining. Results: The FGF-2 gene was transfected into rBMSCs (Multiplicity of infection [MOI] = 100) by lentivirus, and its efficiency was above 95%. The rBMSCs were successfully transfected overexpressing FGF-2 by qPCR and western blot. After tissue engineering bone implantation, X-ray examination and gross specimen observation revealed that the repair area in the E group was > 80% at six weeks, the defect was completely repaired at 12 weeks, and osteogenesis was stronger, when compared with the other groups. For the X-ray score, vascular area density and new bone formation area were higher, when compared with the other groups, and the difference was statistically significant (P< 0.05). Conclusion: FGF-2 gene overexpression lentivirus transfection BMSCs combined with XACB to construct tissue engineered bone can effectively promote vascular regeneration, and improve the repair effect of avascular necrosis of the femoral head.</description><identifier>ISSN: 1015-8987</identifier><identifier>EISSN: 1421-9778</identifier><identifier>DOI: 10.1159/000491906</identifier><identifier>PMID: 30025396</identifier><language>eng</language><publisher>Basel, Switzerland: S. Karger AG</publisher><subject>Animals ; Antigens ; Avascular necrosis of the femoral head ; Basic fibroblast growth factor ; Bone and Bones - diagnostic imaging ; Bone and Bones - pathology ; Bone marrow ; Bone Marrow Cells - cytology ; Bone marrow mesenchymal stem cells ; Bone Regeneration - physiology ; Cells, Cultured ; Disease Models, Animal ; Female ; Femur Head Necrosis - etiology ; Femur Head Necrosis - metabolism ; Femur Head Necrosis - pathology ; Fibroblast Growth Factor 2 - genetics ; Fibroblast Growth Factor 2 - metabolism ; Gangrene ; Genetic Vectors - genetics ; Genetic Vectors - metabolism ; Growth factors ; Laboratory animals ; Lentivirus - genetics ; Lipopolysaccharides - toxicity ; Male ; Mesenchymal Stem Cell Transplantation ; Mesenchymal Stem Cells - cytology ; Mesenchymal Stem Cells - metabolism ; Necrosis ; Original Paper ; Osteogenesis ; Rabbits ; Stem cells ; Tissue Engineering ; Tissue engineering bone</subject><ispartof>Cellular physiology and biochemistry, 2018-01, Vol.48 (2), p.773-784</ispartof><rights>2018 The Author(s). Published by S. Karger AG, Basel</rights><rights>2018 The Author(s). Published by S. Karger AG, Basel.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c529t-d3572a442894302ef0d2d98f007ce04502129876e8576a2a859df9eb442bde6f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,862,2098,27618,27907,27908</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30025396$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Fei</creatorcontrib><creatorcontrib>Peng, Wu-xun </creatorcontrib><creatorcontrib>Wang, Lei</creatorcontrib><creatorcontrib>Zhang, Jian</creatorcontrib><creatorcontrib>Dong, Wen-tao</creatorcontrib><creatorcontrib>Wu, Jian-hua</creatorcontrib><creatorcontrib>Zhang, Huai</creatorcontrib><creatorcontrib>Wang, Jian-bo</creatorcontrib><creatorcontrib>Zhao, Yin</creatorcontrib><title>Role of FGF-2 Transfected Bone Marrow Mesenchymal Stem Cells in Engineered Bone Tissue for Repair of Avascular Necrosis of Femoral Head in Rabbits</title><title>Cellular physiology and biochemistry</title><addtitle>Cell Physiol Biochem</addtitle><description>Background/Aims: Avascular necrosis of the femoral head (ANFH) is the focus and difficulty of orthopedic diseases. Recently, tissue engineering bone for this disease has shown a good therapeutic effect. The aim of the present study was to investigate the therapeutic effect of basic fibroblast growth factor (FGF-2) as cytokines transfected bone marrow mesenchymal stem cells (BMSCs) in constructing tissue-engineered bone for avascular necrosis of the femoral head. Methods: The FGF-2 gene overexpressed lentivirus-transfected rBMSCs with xenogeneic antigen-extracted cancellous bone (XACB) to construct tissue engineered bone, and the model of early avascular necrosis of the femoral head was established by lipopolysaccharide (LPS) combined with hormone. The models were randomly divided into five groups: A (control), B (XACB), C (XACB+rBMSCs), D (XACB+rBMSCs+Lv-GFP), and E (XACB+rBMSCs+Lv-FGF-2/GFP) groups. The therapeutic effect of the tissue engineered bone for the avascular necrosis of the femoral head was evaluated by gross anatomy, X-ray examination, immunohistochemistry and H&E staining. Results: The FGF-2 gene was transfected into rBMSCs (Multiplicity of infection [MOI] = 100) by lentivirus, and its efficiency was above 95%. The rBMSCs were successfully transfected overexpressing FGF-2 by qPCR and western blot. After tissue engineering bone implantation, X-ray examination and gross specimen observation revealed that the repair area in the E group was > 80% at six weeks, the defect was completely repaired at 12 weeks, and osteogenesis was stronger, when compared with the other groups. For the X-ray score, vascular area density and new bone formation area were higher, when compared with the other groups, and the difference was statistically significant (P< 0.05). Conclusion: FGF-2 gene overexpression lentivirus transfection BMSCs combined with XACB to construct tissue engineered bone can effectively promote vascular regeneration, and improve the repair effect of avascular necrosis of the femoral head.</description><subject>Animals</subject><subject>Antigens</subject><subject>Avascular necrosis of the femoral head</subject><subject>Basic fibroblast growth factor</subject><subject>Bone and Bones - diagnostic imaging</subject><subject>Bone and Bones - pathology</subject><subject>Bone marrow</subject><subject>Bone Marrow Cells - cytology</subject><subject>Bone marrow mesenchymal stem cells</subject><subject>Bone Regeneration - physiology</subject><subject>Cells, Cultured</subject><subject>Disease Models, Animal</subject><subject>Female</subject><subject>Femur Head Necrosis - etiology</subject><subject>Femur Head Necrosis - metabolism</subject><subject>Femur Head Necrosis - pathology</subject><subject>Fibroblast Growth Factor 2 - genetics</subject><subject>Fibroblast Growth Factor 2 - metabolism</subject><subject>Gangrene</subject><subject>Genetic Vectors - genetics</subject><subject>Genetic Vectors - metabolism</subject><subject>Growth factors</subject><subject>Laboratory animals</subject><subject>Lentivirus - genetics</subject><subject>Lipopolysaccharides - toxicity</subject><subject>Male</subject><subject>Mesenchymal Stem Cell Transplantation</subject><subject>Mesenchymal Stem Cells - cytology</subject><subject>Mesenchymal Stem Cells - metabolism</subject><subject>Necrosis</subject><subject>Original Paper</subject><subject>Osteogenesis</subject><subject>Rabbits</subject><subject>Stem cells</subject><subject>Tissue Engineering</subject><subject>Tissue engineering bone</subject><issn>1015-8987</issn><issn>1421-9778</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>M--</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DOA</sourceid><recordid>eNptkU1v0zAYgCMEYmNw4I6QJS5wyLAdO46PW7VukzZApZwt135dXJK42Alof4NfjLN2nYQ4-UPP-7xfRfGa4FNCuPyIMWaSSFw_KY4Jo6SUQjRP8x0TXjayEUfFi5Q2OD-FpM-LowpjyitZHxd_FqEFFByaX85LipZR98mBGcCi89ADutUxht_oFhL05vtdp1v0dYAOzaBtE_I9uujXvgeIDwFLn9IIyIWIFrDVPk7ys186mbHVEX0CE0Py6T4ldCFm4RVoO6kWerXyQ3pZPHO6TfBqf54U3-YXy9lVefP58np2dlMaTuVQ2ooLqhmjjWQVpuCwpVY2DmNhADOOKaG59RoaLmpNdcOldRJWOWJloXbVSXG989qgN2obfafjnQraq_uPENdKx8GbFlS2WSu4NgZzRm3TGMcICFxzIRhIml3vd65tDD9HSIPqfDJ5RrqHMCZFsagqymvKMvruH3QTxtjnThUlRBBBK1Zn6sOOmsaVIrhDgQSraenqsPTMvt0bx1UH9kA-bPkx5Q8d1xAPwOzL-U6htnaax5v_UvssfwHeArit</recordid><startdate>20180101</startdate><enddate>20180101</enddate><creator>Zhang, Fei</creator><creator>Peng, Wu-xun </creator><creator>Wang, Lei</creator><creator>Zhang, Jian</creator><creator>Dong, Wen-tao</creator><creator>Wu, Jian-hua</creator><creator>Zhang, Huai</creator><creator>Wang, Jian-bo</creator><creator>Zhao, Yin</creator><general>S. Karger AG</general><general>Cell Physiol Biochem Press GmbH & Co KG</general><scope>M--</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>DOA</scope></search><sort><creationdate>20180101</creationdate><title>Role of FGF-2 Transfected Bone Marrow Mesenchymal Stem Cells in Engineered Bone Tissue for Repair of Avascular Necrosis of Femoral Head in Rabbits</title><author>Zhang, Fei ; Peng, Wu-xun ; Wang, Lei ; Zhang, Jian ; Dong, Wen-tao ; Wu, Jian-hua ; Zhang, Huai ; Wang, Jian-bo ; Zhao, Yin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c529t-d3572a442894302ef0d2d98f007ce04502129876e8576a2a859df9eb442bde6f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Antigens</topic><topic>Avascular necrosis of the femoral head</topic><topic>Basic fibroblast growth factor</topic><topic>Bone and Bones - diagnostic imaging</topic><topic>Bone and Bones - pathology</topic><topic>Bone marrow</topic><topic>Bone Marrow Cells - cytology</topic><topic>Bone marrow mesenchymal stem cells</topic><topic>Bone Regeneration - physiology</topic><topic>Cells, Cultured</topic><topic>Disease Models, Animal</topic><topic>Female</topic><topic>Femur Head Necrosis - etiology</topic><topic>Femur Head Necrosis - metabolism</topic><topic>Femur Head Necrosis - pathology</topic><topic>Fibroblast Growth Factor 2 - genetics</topic><topic>Fibroblast Growth Factor 2 - metabolism</topic><topic>Gangrene</topic><topic>Genetic Vectors - genetics</topic><topic>Genetic Vectors - metabolism</topic><topic>Growth factors</topic><topic>Laboratory animals</topic><topic>Lentivirus - genetics</topic><topic>Lipopolysaccharides - toxicity</topic><topic>Male</topic><topic>Mesenchymal Stem Cell Transplantation</topic><topic>Mesenchymal Stem Cells - cytology</topic><topic>Mesenchymal Stem Cells - metabolism</topic><topic>Necrosis</topic><topic>Original Paper</topic><topic>Osteogenesis</topic><topic>Rabbits</topic><topic>Stem cells</topic><topic>Tissue Engineering</topic><topic>Tissue engineering bone</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Fei</creatorcontrib><creatorcontrib>Peng, Wu-xun </creatorcontrib><creatorcontrib>Wang, Lei</creatorcontrib><creatorcontrib>Zhang, Jian</creatorcontrib><creatorcontrib>Dong, Wen-tao</creatorcontrib><creatorcontrib>Wu, Jian-hua</creatorcontrib><creatorcontrib>Zhang, Huai</creatorcontrib><creatorcontrib>Wang, Jian-bo</creatorcontrib><creatorcontrib>Zhao, Yin</creatorcontrib><collection>Karger Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Cellular physiology and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Fei</au><au>Peng, Wu-xun </au><au>Wang, Lei</au><au>Zhang, Jian</au><au>Dong, Wen-tao</au><au>Wu, Jian-hua</au><au>Zhang, Huai</au><au>Wang, Jian-bo</au><au>Zhao, Yin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of FGF-2 Transfected Bone Marrow Mesenchymal Stem Cells in Engineered Bone Tissue for Repair of Avascular Necrosis of Femoral Head in Rabbits</atitle><jtitle>Cellular physiology and biochemistry</jtitle><addtitle>Cell Physiol Biochem</addtitle><date>2018-01-01</date><risdate>2018</risdate><volume>48</volume><issue>2</issue><spage>773</spage><epage>784</epage><pages>773-784</pages><issn>1015-8987</issn><eissn>1421-9778</eissn><abstract>Background/Aims: Avascular necrosis of the femoral head (ANFH) is the focus and difficulty of orthopedic diseases. Recently, tissue engineering bone for this disease has shown a good therapeutic effect. The aim of the present study was to investigate the therapeutic effect of basic fibroblast growth factor (FGF-2) as cytokines transfected bone marrow mesenchymal stem cells (BMSCs) in constructing tissue-engineered bone for avascular necrosis of the femoral head. Methods: The FGF-2 gene overexpressed lentivirus-transfected rBMSCs with xenogeneic antigen-extracted cancellous bone (XACB) to construct tissue engineered bone, and the model of early avascular necrosis of the femoral head was established by lipopolysaccharide (LPS) combined with hormone. The models were randomly divided into five groups: A (control), B (XACB), C (XACB+rBMSCs), D (XACB+rBMSCs+Lv-GFP), and E (XACB+rBMSCs+Lv-FGF-2/GFP) groups. The therapeutic effect of the tissue engineered bone for the avascular necrosis of the femoral head was evaluated by gross anatomy, X-ray examination, immunohistochemistry and H&E staining. Results: The FGF-2 gene was transfected into rBMSCs (Multiplicity of infection [MOI] = 100) by lentivirus, and its efficiency was above 95%. The rBMSCs were successfully transfected overexpressing FGF-2 by qPCR and western blot. After tissue engineering bone implantation, X-ray examination and gross specimen observation revealed that the repair area in the E group was > 80% at six weeks, the defect was completely repaired at 12 weeks, and osteogenesis was stronger, when compared with the other groups. For the X-ray score, vascular area density and new bone formation area were higher, when compared with the other groups, and the difference was statistically significant (P< 0.05). Conclusion: FGF-2 gene overexpression lentivirus transfection BMSCs combined with XACB to construct tissue engineered bone can effectively promote vascular regeneration, and improve the repair effect of avascular necrosis of the femoral head.</abstract><cop>Basel, Switzerland</cop><pub>S. Karger AG</pub><pmid>30025396</pmid><doi>10.1159/000491906</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Antigens Avascular necrosis of the femoral head Basic fibroblast growth factor Bone and Bones - diagnostic imaging Bone and Bones - pathology Bone marrow Bone Marrow Cells - cytology Bone marrow mesenchymal stem cells Bone Regeneration - physiology Cells, Cultured Disease Models, Animal Female Femur Head Necrosis - etiology Femur Head Necrosis - metabolism Femur Head Necrosis - pathology Fibroblast Growth Factor 2 - genetics Fibroblast Growth Factor 2 - metabolism Gangrene Genetic Vectors - genetics Genetic Vectors - metabolism Growth factors Laboratory animals Lentivirus - genetics Lipopolysaccharides - toxicity Male Mesenchymal Stem Cell Transplantation Mesenchymal Stem Cells - cytology Mesenchymal Stem Cells - metabolism Necrosis Original Paper Osteogenesis Rabbits Stem cells Tissue Engineering Tissue engineering bone |
| title | Role of FGF-2 Transfected Bone Marrow Mesenchymal Stem Cells in Engineered Bone Tissue for Repair of Avascular Necrosis of Femoral Head in Rabbits |
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