Atherosclerotic patients have lower levels of BLTR1 expressing microvesicles compared to healthy individuals
Background: Monocytes/macrophages play a crucial role in the development, progression, and complication of atherosclerosis. In particular, foam cell formation driven by CD36 mediated internalisation of oxLDL leads to activation of monocytes and subsequent release of monocytederived microvesicles (MM...
Gespeichert in:
| Veröffentlicht in: | Journal of extracellular vesicles 2018-01, Vol.7, p.71-71 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 71 |
|---|---|
| container_issue | |
| container_start_page | 71 |
| container_title | Journal of extracellular vesicles |
| container_volume | 7 |
| creator | Sanden, Mathilde Botha, Jaco Nielsen, Michael René Skjelbo Nielsen, Morten Hjuler Schmidt, Erik Berg Handberg, Aase |
| description | Background: Monocytes/macrophages play a crucial role in the development, progression, and complication of atherosclerosis. In particular, foam cell formation driven by CD36 mediated internalisation of oxLDL leads to activation of monocytes and subsequent release of monocytederived microvesicles (MMVs). Further, pro-inflammatory leukotriene B4 derived from arachidonic acid (AA) promotes atherosclerosis through the high-affinity receptor BLTR1. Thus, we aimed to investigate the correlation between different MMV phenotypes on the one hand, and AA and eicosapentaenoic acid (EPA) contents in different compartments including atherosclerotic plaques, plasma and granulocytes on the other. This might elucidate the potential of CD36 and BLTR1 bearing MMV phenotypes as novel biomarkers in predicting atherosclerosis. Methods: Plasma samples from 48 subjects with femoral atherosclerosis and 24 healthy controls were analysed on an Apogee A60 MicroPLUS flow cytometer. Platelet-poor plasma was labelled with lactadherin-FITC, anti-CD14-APC, anti-CD36-PE and anti-BLTR1-AF700. MVs were defined as phosphatidylserine-exposing (PS+) events |
| format | Article |
| fullrecord | <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_journals_2116609995</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2116609995</sourcerecordid><originalsourceid>FETCH-proquest_journals_21166099953</originalsourceid><addsrcrecordid>eNqNjL0KwjAUhYMgKNp3uOAsJP7UZlRRHJzEXUJ7tSlpUnPTqG9vBh_AM5xv-A5nwMYLzsV8yTfFiGVEDU-RK7Eu5JiZbajROypN6qBL6FTQaANBrSKCcS_0YDCiIXB32J2vFwH47jwSafuAVpfeRSSdDghK13bKYwXBQY3KhPoD2lY66qpXhqZseE_A7McJmx0P1_1p3nn37JHCrXG9t0ndFkLkOZdSrpf_rb4rp0px</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2116609995</pqid></control><display><type>article</type><title>Atherosclerotic patients have lower levels of BLTR1 expressing microvesicles compared to healthy individuals</title><source>Taylor & Francis Open Access</source><source>Wiley Journals</source><source>DOAJ Directory of Open Access Journals</source><source>Co-Action Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Wiley Online Library (Open Access Collection)</source><source>PubMed Central</source><creator>Sanden, Mathilde ; Botha, Jaco ; Nielsen, Michael René Skjelbo ; Nielsen, Morten Hjuler ; Schmidt, Erik Berg ; Handberg, Aase</creator><creatorcontrib>Sanden, Mathilde ; Botha, Jaco ; Nielsen, Michael René Skjelbo ; Nielsen, Morten Hjuler ; Schmidt, Erik Berg ; Handberg, Aase</creatorcontrib><description>Background: Monocytes/macrophages play a crucial role in the development, progression, and complication of atherosclerosis. In particular, foam cell formation driven by CD36 mediated internalisation of oxLDL leads to activation of monocytes and subsequent release of monocytederived microvesicles (MMVs). Further, pro-inflammatory leukotriene B4 derived from arachidonic acid (AA) promotes atherosclerosis through the high-affinity receptor BLTR1. Thus, we aimed to investigate the correlation between different MMV phenotypes on the one hand, and AA and eicosapentaenoic acid (EPA) contents in different compartments including atherosclerotic plaques, plasma and granulocytes on the other. This might elucidate the potential of CD36 and BLTR1 bearing MMV phenotypes as novel biomarkers in predicting atherosclerosis. Methods: Plasma samples from 48 subjects with femoral atherosclerosis and 24 healthy controls were analysed on an Apogee A60 MicroPLUS flow cytometer. Platelet-poor plasma was labelled with lactadherin-FITC, anti-CD14-APC, anti-CD36-PE and anti-BLTR1-AF700. MVs were defined as phosphatidylserine-exposing (PS+) events <1000 nm in size. EPA and AA content in granulocytes, plasma phospholipids and atherosclerotic plaques were analysed using gas chromatography. Results: Patients with atherosclerosis had lower levels of BLTR1+ MVs (p = 0.007), CD14+BLTR1+ MVs (p = 0.007) and CD14+BLTR1+CD36+ MVs (p = 0.001) compared to healthy controls. Further, CD14+ MVs and CD14+CD36+ MVs correlated negatively with AA in granulocytes (r = -0.302, p = 0.039 and r = -0.322, p = 0.028, respectively). CD14 +CD36+ MVs correlated negatively with AA in plasma phospholipids (r = -0.315, p = 0.029). Lastly, CD14+ MVs and CD14+BLTR1+ MVs tended to correlate inversely with AA in plasma phospholipids and AA in atherosclerotic plaques, respectively (r = -0.284, p = 0.050 and r = - 0.291, p = 0.058). Summary/Conclusion: We demonstrated that atherosclerotic patients had lower levels of BLTR1+ MV phenotypes compared to healthy individuals. This is possibly caused by higher levels of LTB4 and inflammatory cytokines in patients which leads to receptor downregulation. Further investigations into the origin and phenotype may support the potential of circulating MVs in early diagnosis of atherosclerosis.</description><identifier>EISSN: 2001-3078</identifier><language>eng</language><publisher>Abingdon: John Wiley & Sons, Inc</publisher><subject>Arachidonic acid ; Arteriosclerosis ; Atherosclerosis ; CD14 antigen ; CD36 antigen ; Cell activation ; Cytokines ; Eicosapentaenoic acid ; Femur ; Gas chromatography ; Granulocytes ; Inflammation ; Leukocytes (granulocytic) ; Leukotriene B4 ; Macrophages ; Monocytes ; Phenotypes ; Phosphatidylserine ; Phospholipids ; Plaques ; Plasma</subject><ispartof>Journal of extracellular vesicles, 2018-01, Vol.7, p.71-71</ispartof><rights>Copyright Taylor & Francis Ltd. 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>Sanden, Mathilde</creatorcontrib><creatorcontrib>Botha, Jaco</creatorcontrib><creatorcontrib>Nielsen, Michael René Skjelbo</creatorcontrib><creatorcontrib>Nielsen, Morten Hjuler</creatorcontrib><creatorcontrib>Schmidt, Erik Berg</creatorcontrib><creatorcontrib>Handberg, Aase</creatorcontrib><title>Atherosclerotic patients have lower levels of BLTR1 expressing microvesicles compared to healthy individuals</title><title>Journal of extracellular vesicles</title><description>Background: Monocytes/macrophages play a crucial role in the development, progression, and complication of atherosclerosis. In particular, foam cell formation driven by CD36 mediated internalisation of oxLDL leads to activation of monocytes and subsequent release of monocytederived microvesicles (MMVs). Further, pro-inflammatory leukotriene B4 derived from arachidonic acid (AA) promotes atherosclerosis through the high-affinity receptor BLTR1. Thus, we aimed to investigate the correlation between different MMV phenotypes on the one hand, and AA and eicosapentaenoic acid (EPA) contents in different compartments including atherosclerotic plaques, plasma and granulocytes on the other. This might elucidate the potential of CD36 and BLTR1 bearing MMV phenotypes as novel biomarkers in predicting atherosclerosis. Methods: Plasma samples from 48 subjects with femoral atherosclerosis and 24 healthy controls were analysed on an Apogee A60 MicroPLUS flow cytometer. Platelet-poor plasma was labelled with lactadherin-FITC, anti-CD14-APC, anti-CD36-PE and anti-BLTR1-AF700. MVs were defined as phosphatidylserine-exposing (PS+) events <1000 nm in size. EPA and AA content in granulocytes, plasma phospholipids and atherosclerotic plaques were analysed using gas chromatography. Results: Patients with atherosclerosis had lower levels of BLTR1+ MVs (p = 0.007), CD14+BLTR1+ MVs (p = 0.007) and CD14+BLTR1+CD36+ MVs (p = 0.001) compared to healthy controls. Further, CD14+ MVs and CD14+CD36+ MVs correlated negatively with AA in granulocytes (r = -0.302, p = 0.039 and r = -0.322, p = 0.028, respectively). CD14 +CD36+ MVs correlated negatively with AA in plasma phospholipids (r = -0.315, p = 0.029). Lastly, CD14+ MVs and CD14+BLTR1+ MVs tended to correlate inversely with AA in plasma phospholipids and AA in atherosclerotic plaques, respectively (r = -0.284, p = 0.050 and r = - 0.291, p = 0.058). Summary/Conclusion: We demonstrated that atherosclerotic patients had lower levels of BLTR1+ MV phenotypes compared to healthy individuals. This is possibly caused by higher levels of LTB4 and inflammatory cytokines in patients which leads to receptor downregulation. Further investigations into the origin and phenotype may support the potential of circulating MVs in early diagnosis of atherosclerosis.</description><subject>Arachidonic acid</subject><subject>Arteriosclerosis</subject><subject>Atherosclerosis</subject><subject>CD14 antigen</subject><subject>CD36 antigen</subject><subject>Cell activation</subject><subject>Cytokines</subject><subject>Eicosapentaenoic acid</subject><subject>Femur</subject><subject>Gas chromatography</subject><subject>Granulocytes</subject><subject>Inflammation</subject><subject>Leukocytes (granulocytic)</subject><subject>Leukotriene B4</subject><subject>Macrophages</subject><subject>Monocytes</subject><subject>Phenotypes</subject><subject>Phosphatidylserine</subject><subject>Phospholipids</subject><subject>Plaques</subject><subject>Plasma</subject><issn>2001-3078</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNjL0KwjAUhYMgKNp3uOAsJP7UZlRRHJzEXUJ7tSlpUnPTqG9vBh_AM5xv-A5nwMYLzsV8yTfFiGVEDU-RK7Eu5JiZbajROypN6qBL6FTQaANBrSKCcS_0YDCiIXB32J2vFwH47jwSafuAVpfeRSSdDghK13bKYwXBQY3KhPoD2lY66qpXhqZseE_A7McJmx0P1_1p3nn37JHCrXG9t0ndFkLkOZdSrpf_rb4rp0px</recordid><startdate>20180101</startdate><enddate>20180101</enddate><creator>Sanden, Mathilde</creator><creator>Botha, Jaco</creator><creator>Nielsen, Michael René Skjelbo</creator><creator>Nielsen, Morten Hjuler</creator><creator>Schmidt, Erik Berg</creator><creator>Handberg, Aase</creator><general>John Wiley & Sons, Inc</general><scope>7QP</scope><scope>8FE</scope><scope>8FH</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20180101</creationdate><title>Atherosclerotic patients have lower levels of BLTR1 expressing microvesicles compared to healthy individuals</title><author>Sanden, Mathilde ; Botha, Jaco ; Nielsen, Michael René Skjelbo ; Nielsen, Morten Hjuler ; Schmidt, Erik Berg ; Handberg, Aase</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_journals_21166099953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Arachidonic acid</topic><topic>Arteriosclerosis</topic><topic>Atherosclerosis</topic><topic>CD14 antigen</topic><topic>CD36 antigen</topic><topic>Cell activation</topic><topic>Cytokines</topic><topic>Eicosapentaenoic acid</topic><topic>Femur</topic><topic>Gas chromatography</topic><topic>Granulocytes</topic><topic>Inflammation</topic><topic>Leukocytes (granulocytic)</topic><topic>Leukotriene B4</topic><topic>Macrophages</topic><topic>Monocytes</topic><topic>Phenotypes</topic><topic>Phosphatidylserine</topic><topic>Phospholipids</topic><topic>Plaques</topic><topic>Plasma</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sanden, Mathilde</creatorcontrib><creatorcontrib>Botha, Jaco</creatorcontrib><creatorcontrib>Nielsen, Michael René Skjelbo</creatorcontrib><creatorcontrib>Nielsen, Morten Hjuler</creatorcontrib><creatorcontrib>Schmidt, Erik Berg</creatorcontrib><creatorcontrib>Handberg, Aase</creatorcontrib><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Journal of extracellular vesicles</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sanden, Mathilde</au><au>Botha, Jaco</au><au>Nielsen, Michael René Skjelbo</au><au>Nielsen, Morten Hjuler</au><au>Schmidt, Erik Berg</au><au>Handberg, Aase</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Atherosclerotic patients have lower levels of BLTR1 expressing microvesicles compared to healthy individuals</atitle><jtitle>Journal of extracellular vesicles</jtitle><date>2018-01-01</date><risdate>2018</risdate><volume>7</volume><spage>71</spage><epage>71</epage><pages>71-71</pages><eissn>2001-3078</eissn><abstract>Background: Monocytes/macrophages play a crucial role in the development, progression, and complication of atherosclerosis. In particular, foam cell formation driven by CD36 mediated internalisation of oxLDL leads to activation of monocytes and subsequent release of monocytederived microvesicles (MMVs). Further, pro-inflammatory leukotriene B4 derived from arachidonic acid (AA) promotes atherosclerosis through the high-affinity receptor BLTR1. Thus, we aimed to investigate the correlation between different MMV phenotypes on the one hand, and AA and eicosapentaenoic acid (EPA) contents in different compartments including atherosclerotic plaques, plasma and granulocytes on the other. This might elucidate the potential of CD36 and BLTR1 bearing MMV phenotypes as novel biomarkers in predicting atherosclerosis. Methods: Plasma samples from 48 subjects with femoral atherosclerosis and 24 healthy controls were analysed on an Apogee A60 MicroPLUS flow cytometer. Platelet-poor plasma was labelled with lactadherin-FITC, anti-CD14-APC, anti-CD36-PE and anti-BLTR1-AF700. MVs were defined as phosphatidylserine-exposing (PS+) events <1000 nm in size. EPA and AA content in granulocytes, plasma phospholipids and atherosclerotic plaques were analysed using gas chromatography. Results: Patients with atherosclerosis had lower levels of BLTR1+ MVs (p = 0.007), CD14+BLTR1+ MVs (p = 0.007) and CD14+BLTR1+CD36+ MVs (p = 0.001) compared to healthy controls. Further, CD14+ MVs and CD14+CD36+ MVs correlated negatively with AA in granulocytes (r = -0.302, p = 0.039 and r = -0.322, p = 0.028, respectively). CD14 +CD36+ MVs correlated negatively with AA in plasma phospholipids (r = -0.315, p = 0.029). Lastly, CD14+ MVs and CD14+BLTR1+ MVs tended to correlate inversely with AA in plasma phospholipids and AA in atherosclerotic plaques, respectively (r = -0.284, p = 0.050 and r = - 0.291, p = 0.058). Summary/Conclusion: We demonstrated that atherosclerotic patients had lower levels of BLTR1+ MV phenotypes compared to healthy individuals. This is possibly caused by higher levels of LTB4 and inflammatory cytokines in patients which leads to receptor downregulation. Further investigations into the origin and phenotype may support the potential of circulating MVs in early diagnosis of atherosclerosis.</abstract><cop>Abingdon</cop><pub>John Wiley & Sons, Inc</pub></addata></record> |
| fulltext | fulltext |
| identifier | EISSN: 2001-3078 |
| ispartof | Journal of extracellular vesicles, 2018-01, Vol.7, p.71-71 |
| issn | 2001-3078 |
| language | eng |
| recordid | cdi_proquest_journals_2116609995 |
| source | Taylor & Francis Open Access; Wiley Journals; DOAJ Directory of Open Access Journals; Co-Action Open Access Journals; EZB-FREE-00999 freely available EZB journals; Wiley Online Library (Open Access Collection); PubMed Central |
| subjects | Arachidonic acid Arteriosclerosis Atherosclerosis CD14 antigen CD36 antigen Cell activation Cytokines Eicosapentaenoic acid Femur Gas chromatography Granulocytes Inflammation Leukocytes (granulocytic) Leukotriene B4 Macrophages Monocytes Phenotypes Phosphatidylserine Phospholipids Plaques Plasma |
| title | Atherosclerotic patients have lower levels of BLTR1 expressing microvesicles compared to healthy individuals |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T06%3A33%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Atherosclerotic%20patients%20have%20lower%20levels%20of%20BLTR1%20expressing%20microvesicles%20compared%20to%20healthy%20individuals&rft.jtitle=Journal%20of%20extracellular%20vesicles&rft.au=Sanden,%20Mathilde&rft.date=2018-01-01&rft.volume=7&rft.spage=71&rft.epage=71&rft.pages=71-71&rft.eissn=2001-3078&rft_id=info:doi/&rft_dat=%3Cproquest%3E2116609995%3C/proquest%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2116609995&rft_id=info:pmid/&rfr_iscdi=true |