Silicon membrane filter designed by fluid dynamics simulation and near-field stress analysis for selective cell enrichment
Selective cell enrichment technologies can play an important role in both diagnostic and therapeutic areas. However, currently used cell sorting techniques have difficulties in rapidly isolating only the desired target cells from a large volume of body fluids. In this work, we developed a filtering...
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| Veröffentlicht in: | Biomedical microdevices 2018-12, Vol.20 (4), p.87, Article 87 |
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| creator | Jang, Yo-Chang Park, Hyun-Ju Woo, Ayoung Lee, Kyu-Sung Moon, Hui-Sung Oh, Jin Ho Lee, Min-Young |
| description | Selective cell enrichment technologies can play an important role in both diagnostic and therapeutic areas. However, currently used cell sorting techniques have difficulties in rapidly isolating only the desired target cells from a large volume of body fluids. In this work, we developed a filtering system that can quickly separate and highly concentrate cells from a large volume of solution, depending on their size, using a silicon membrane filter. To overcome the problems caused by material limitations of the brittle silicon, we designed a novel membrane filter with various pore designs. From these designs, the most optimal design with high pore density, while preventing crack formation was derived by applying fluid dynamics simulation and near-field stress analysis. The membrane filter system using the selected design was fabricated, and cell filtration performance was evaluated. The LNCaP cell in horse blood was recovered up to 86% and enriched to 187-fold compared to initial cell populations after filtration at a flow rate of 5 mL/min. The results demonstrate that the filter presented in this study can rapidly and selectively isolate target cells from a large volume of body fluid sample. |
| doi_str_mv | 10.1007/s10544-018-0334-0 |
| format | Article |
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However, currently used cell sorting techniques have difficulties in rapidly isolating only the desired target cells from a large volume of body fluids. In this work, we developed a filtering system that can quickly separate and highly concentrate cells from a large volume of solution, depending on their size, using a silicon membrane filter. To overcome the problems caused by material limitations of the brittle silicon, we designed a novel membrane filter with various pore designs. From these designs, the most optimal design with high pore density, while preventing crack formation was derived by applying fluid dynamics simulation and near-field stress analysis. The membrane filter system using the selected design was fabricated, and cell filtration performance was evaluated. The LNCaP cell in horse blood was recovered up to 86% and enriched to 187-fold compared to initial cell populations after filtration at a flow rate of 5 mL/min. The results demonstrate that the filter presented in this study can rapidly and selectively isolate target cells from a large volume of body fluid sample.</description><identifier>ISSN: 1387-2176</identifier><identifier>EISSN: 1572-8781</identifier><identifier>DOI: 10.1007/s10544-018-0334-0</identifier><identifier>PMID: 30291460</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Biological and Medical Physics ; Biomedical Engineering and Bioengineering ; Biophysics ; Body fluids ; Design ; Diagnostic systems ; Engineering ; Engineering Fluid Dynamics ; Enrichment ; Filtration ; Flow velocity ; Fluid dynamics ; Hydrodynamics ; Membrane filters ; Nanotechnology ; Silicon ; Simulation ; Stress analysis</subject><ispartof>Biomedical microdevices, 2018-12, Vol.20 (4), p.87, Article 87</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2018</rights><rights>Biomedical Microdevices is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c328t-2c19b8c5847a4a55c0fd0d1801b127418d7e423dbbbcabdc118a18cfa04072d63</cites><orcidid>0000-0003-3396-7632</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10544-018-0334-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10544-018-0334-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30291460$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jang, Yo-Chang</creatorcontrib><creatorcontrib>Park, Hyun-Ju</creatorcontrib><creatorcontrib>Woo, Ayoung</creatorcontrib><creatorcontrib>Lee, Kyu-Sung</creatorcontrib><creatorcontrib>Moon, Hui-Sung</creatorcontrib><creatorcontrib>Oh, Jin Ho</creatorcontrib><creatorcontrib>Lee, Min-Young</creatorcontrib><title>Silicon membrane filter designed by fluid dynamics simulation and near-field stress analysis for selective cell enrichment</title><title>Biomedical microdevices</title><addtitle>Biomed Microdevices</addtitle><addtitle>Biomed Microdevices</addtitle><description>Selective cell enrichment technologies can play an important role in both diagnostic and therapeutic areas. However, currently used cell sorting techniques have difficulties in rapidly isolating only the desired target cells from a large volume of body fluids. In this work, we developed a filtering system that can quickly separate and highly concentrate cells from a large volume of solution, depending on their size, using a silicon membrane filter. To overcome the problems caused by material limitations of the brittle silicon, we designed a novel membrane filter with various pore designs. From these designs, the most optimal design with high pore density, while preventing crack formation was derived by applying fluid dynamics simulation and near-field stress analysis. The membrane filter system using the selected design was fabricated, and cell filtration performance was evaluated. The LNCaP cell in horse blood was recovered up to 86% and enriched to 187-fold compared to initial cell populations after filtration at a flow rate of 5 mL/min. 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However, currently used cell sorting techniques have difficulties in rapidly isolating only the desired target cells from a large volume of body fluids. In this work, we developed a filtering system that can quickly separate and highly concentrate cells from a large volume of solution, depending on their size, using a silicon membrane filter. To overcome the problems caused by material limitations of the brittle silicon, we designed a novel membrane filter with various pore designs. From these designs, the most optimal design with high pore density, while preventing crack formation was derived by applying fluid dynamics simulation and near-field stress analysis. The membrane filter system using the selected design was fabricated, and cell filtration performance was evaluated. The LNCaP cell in horse blood was recovered up to 86% and enriched to 187-fold compared to initial cell populations after filtration at a flow rate of 5 mL/min. 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| subjects | Biological and Medical Physics Biomedical Engineering and Bioengineering Biophysics Body fluids Design Diagnostic systems Engineering Engineering Fluid Dynamics Enrichment Filtration Flow velocity Fluid dynamics Hydrodynamics Membrane filters Nanotechnology Silicon Simulation Stress analysis |
| title | Silicon membrane filter designed by fluid dynamics simulation and near-field stress analysis for selective cell enrichment |
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