Enhancement of Activity of Carbohydrases with Endo-depolymerase Action in Trichoderma reesei Using Mutagenesis
— In order to obtain feed enzyme preparations with increased activity of endo-carbohydrases of depolymerase action, mutagenesis of the Trichoderma reesei strain was carried out using ultraviolet and ionizing irradiation. Application of fractionated UV-irradiation made it possible to increase the end...
Gespeichert in:
| Veröffentlicht in: | Microbiology (New York) 2018-09, Vol.87 (5), p.652-661 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 661 |
|---|---|
| container_issue | 5 |
| container_start_page | 652 |
| container_title | Microbiology (New York) |
| container_volume | 87 |
| creator | Kostyleva, E. V. Tsurikova, N. V. Sereda, A. S. Velikoretskaya, I. A. Veselkina, T. N. Lobanov, N. S. Shashkov, I. A. Sinitsyn, A. P. |
| description | —
In order to obtain feed enzyme preparations with increased activity of endo-carbohydrases of depolymerase action, mutagenesis of the
Trichoderma reesei
strain was carried out using ultraviolet and ionizing irradiation. Application of fractionated UV-irradiation made it possible to increase the endoglucanase activity by 20% and xylanase activity by 80% compared to the original strain. The greatest effect was achieved after multistage gamma-irradiation mutagenesis with a cobalt source. When the mutant
T. reesei-Co-44
was cultured in shaking flasks, its endoglucanase activity was increased 5-fold, while xylanase activity increased more than 8-fold compared to the original strain. High activity of the target enzymes in the mutant strains was confirmed by the results of fed-batch cultivation in laboratory reactors with lactose feeding as a carbon source and an inducer of cellulases and xylanases biosynthesis. The maximum activity of endoglucanase and xylanase was achieved by culturing strain
T. reesei-Co-44
obtained as a result of gamma-irradiation mutagenesis and amounted to 1324.2 ± 70.1 and 3394 ± 164 U/mL, respectively. This activity level makes it possible to produce competitive enzyme preparations of carbohydrases for fodder production. |
| doi_str_mv | 10.1134/S0026261718050120 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2116083469</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2116083469</sourcerecordid><originalsourceid>FETCH-LOGICAL-c316t-890d2678c7bbf5b1e90533ddf7f95465e94bcc584c1cad96bc334282a1a5a79f3</originalsourceid><addsrcrecordid>eNp1kM1OwzAQhC0EEqXwANwscQ74J3aSY1UVilTEgfYcOfamcdXYxU5BeXsSisQBcdrVznyz0iB0S8k9pTx9eCOESSZpRnMiCGXkDE2oJHnCGc_O0WSUk1G_RFcx7gghggkxQW7hGuU0tOA67Gs80539sF0_7nMVKt_0JqgIEX_arsELZ3xi4OD3fQvj_RvwDluH18HqxhsIrcIBIILFm2jdFr8cO7UFB9HGa3RRq32Em585RZvHxXq-TFavT8_z2SrRnMouyQtimMxynVVVLSoKBRGcG1NndSFSKaBIK61FnmqqlSlkpTlPWc4UVUJlRc2n6O6Uewj-_QixK3f-GNzwsmR07IWnshhc9OTSwccYoC4PwbYq9CUl5Vhr-afWgWEnJg5et4Xwm_w_9AVPn3qD</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2116083469</pqid></control><display><type>article</type><title>Enhancement of Activity of Carbohydrases with Endo-depolymerase Action in Trichoderma reesei Using Mutagenesis</title><source>SpringerLink Journals</source><creator>Kostyleva, E. V. ; Tsurikova, N. V. ; Sereda, A. S. ; Velikoretskaya, I. A. ; Veselkina, T. N. ; Lobanov, N. S. ; Shashkov, I. A. ; Sinitsyn, A. P.</creator><creatorcontrib>Kostyleva, E. V. ; Tsurikova, N. V. ; Sereda, A. S. ; Velikoretskaya, I. A. ; Veselkina, T. N. ; Lobanov, N. S. ; Shashkov, I. A. ; Sinitsyn, A. P.</creatorcontrib><description>—
In order to obtain feed enzyme preparations with increased activity of endo-carbohydrases of depolymerase action, mutagenesis of the
Trichoderma reesei
strain was carried out using ultraviolet and ionizing irradiation. Application of fractionated UV-irradiation made it possible to increase the endoglucanase activity by 20% and xylanase activity by 80% compared to the original strain. The greatest effect was achieved after multistage gamma-irradiation mutagenesis with a cobalt source. When the mutant
T. reesei-Co-44
was cultured in shaking flasks, its endoglucanase activity was increased 5-fold, while xylanase activity increased more than 8-fold compared to the original strain. High activity of the target enzymes in the mutant strains was confirmed by the results of fed-batch cultivation in laboratory reactors with lactose feeding as a carbon source and an inducer of cellulases and xylanases biosynthesis. The maximum activity of endoglucanase and xylanase was achieved by culturing strain
T. reesei-Co-44
obtained as a result of gamma-irradiation mutagenesis and amounted to 1324.2 ± 70.1 and 3394 ± 164 U/mL, respectively. This activity level makes it possible to produce competitive enzyme preparations of carbohydrases for fodder production.</description><identifier>ISSN: 0026-2617</identifier><identifier>EISSN: 1608-3237</identifier><identifier>DOI: 10.1134/S0026261718050120</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>Batch culture ; Biomedical and Life Sciences ; Carbon sources ; Cobalt ; Endoglucanase ; Enzymes ; Experimental Articles ; Lactose ; Life Sciences ; Medical Microbiology ; Microbiology ; Mutagenesis ; Trichoderma reesei ; Ultraviolet radiation</subject><ispartof>Microbiology (New York), 2018-09, Vol.87 (5), p.652-661</ispartof><rights>Pleiades Publishing, Ltd. 2018</rights><rights>Copyright Springer Science & Business Media 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-890d2678c7bbf5b1e90533ddf7f95465e94bcc584c1cad96bc334282a1a5a79f3</citedby><cites>FETCH-LOGICAL-c316t-890d2678c7bbf5b1e90533ddf7f95465e94bcc584c1cad96bc334282a1a5a79f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S0026261718050120$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S0026261718050120$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids></links><search><creatorcontrib>Kostyleva, E. V.</creatorcontrib><creatorcontrib>Tsurikova, N. V.</creatorcontrib><creatorcontrib>Sereda, A. S.</creatorcontrib><creatorcontrib>Velikoretskaya, I. A.</creatorcontrib><creatorcontrib>Veselkina, T. N.</creatorcontrib><creatorcontrib>Lobanov, N. S.</creatorcontrib><creatorcontrib>Shashkov, I. A.</creatorcontrib><creatorcontrib>Sinitsyn, A. P.</creatorcontrib><title>Enhancement of Activity of Carbohydrases with Endo-depolymerase Action in Trichoderma reesei Using Mutagenesis</title><title>Microbiology (New York)</title><addtitle>Microbiology</addtitle><description>—
In order to obtain feed enzyme preparations with increased activity of endo-carbohydrases of depolymerase action, mutagenesis of the
Trichoderma reesei
strain was carried out using ultraviolet and ionizing irradiation. Application of fractionated UV-irradiation made it possible to increase the endoglucanase activity by 20% and xylanase activity by 80% compared to the original strain. The greatest effect was achieved after multistage gamma-irradiation mutagenesis with a cobalt source. When the mutant
T. reesei-Co-44
was cultured in shaking flasks, its endoglucanase activity was increased 5-fold, while xylanase activity increased more than 8-fold compared to the original strain. High activity of the target enzymes in the mutant strains was confirmed by the results of fed-batch cultivation in laboratory reactors with lactose feeding as a carbon source and an inducer of cellulases and xylanases biosynthesis. The maximum activity of endoglucanase and xylanase was achieved by culturing strain
T. reesei-Co-44
obtained as a result of gamma-irradiation mutagenesis and amounted to 1324.2 ± 70.1 and 3394 ± 164 U/mL, respectively. This activity level makes it possible to produce competitive enzyme preparations of carbohydrases for fodder production.</description><subject>Batch culture</subject><subject>Biomedical and Life Sciences</subject><subject>Carbon sources</subject><subject>Cobalt</subject><subject>Endoglucanase</subject><subject>Enzymes</subject><subject>Experimental Articles</subject><subject>Lactose</subject><subject>Life Sciences</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Mutagenesis</subject><subject>Trichoderma reesei</subject><subject>Ultraviolet radiation</subject><issn>0026-2617</issn><issn>1608-3237</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1kM1OwzAQhC0EEqXwANwscQ74J3aSY1UVilTEgfYcOfamcdXYxU5BeXsSisQBcdrVznyz0iB0S8k9pTx9eCOESSZpRnMiCGXkDE2oJHnCGc_O0WSUk1G_RFcx7gghggkxQW7hGuU0tOA67Gs80539sF0_7nMVKt_0JqgIEX_arsELZ3xi4OD3fQvj_RvwDluH18HqxhsIrcIBIILFm2jdFr8cO7UFB9HGa3RRq32Em585RZvHxXq-TFavT8_z2SrRnMouyQtimMxynVVVLSoKBRGcG1NndSFSKaBIK61FnmqqlSlkpTlPWc4UVUJlRc2n6O6Uewj-_QixK3f-GNzwsmR07IWnshhc9OTSwccYoC4PwbYq9CUl5Vhr-afWgWEnJg5et4Xwm_w_9AVPn3qD</recordid><startdate>20180901</startdate><enddate>20180901</enddate><creator>Kostyleva, E. V.</creator><creator>Tsurikova, N. V.</creator><creator>Sereda, A. S.</creator><creator>Velikoretskaya, I. A.</creator><creator>Veselkina, T. N.</creator><creator>Lobanov, N. S.</creator><creator>Shashkov, I. A.</creator><creator>Sinitsyn, A. P.</creator><general>Pleiades Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20180901</creationdate><title>Enhancement of Activity of Carbohydrases with Endo-depolymerase Action in Trichoderma reesei Using Mutagenesis</title><author>Kostyleva, E. V. ; Tsurikova, N. V. ; Sereda, A. S. ; Velikoretskaya, I. A. ; Veselkina, T. N. ; Lobanov, N. S. ; Shashkov, I. A. ; Sinitsyn, A. P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-890d2678c7bbf5b1e90533ddf7f95465e94bcc584c1cad96bc334282a1a5a79f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Batch culture</topic><topic>Biomedical and Life Sciences</topic><topic>Carbon sources</topic><topic>Cobalt</topic><topic>Endoglucanase</topic><topic>Enzymes</topic><topic>Experimental Articles</topic><topic>Lactose</topic><topic>Life Sciences</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>Mutagenesis</topic><topic>Trichoderma reesei</topic><topic>Ultraviolet radiation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kostyleva, E. V.</creatorcontrib><creatorcontrib>Tsurikova, N. V.</creatorcontrib><creatorcontrib>Sereda, A. S.</creatorcontrib><creatorcontrib>Velikoretskaya, I. A.</creatorcontrib><creatorcontrib>Veselkina, T. N.</creatorcontrib><creatorcontrib>Lobanov, N. S.</creatorcontrib><creatorcontrib>Shashkov, I. A.</creatorcontrib><creatorcontrib>Sinitsyn, A. P.</creatorcontrib><collection>CrossRef</collection><jtitle>Microbiology (New York)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kostyleva, E. V.</au><au>Tsurikova, N. V.</au><au>Sereda, A. S.</au><au>Velikoretskaya, I. A.</au><au>Veselkina, T. N.</au><au>Lobanov, N. S.</au><au>Shashkov, I. A.</au><au>Sinitsyn, A. P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhancement of Activity of Carbohydrases with Endo-depolymerase Action in Trichoderma reesei Using Mutagenesis</atitle><jtitle>Microbiology (New York)</jtitle><stitle>Microbiology</stitle><date>2018-09-01</date><risdate>2018</risdate><volume>87</volume><issue>5</issue><spage>652</spage><epage>661</epage><pages>652-661</pages><issn>0026-2617</issn><eissn>1608-3237</eissn><abstract>—
In order to obtain feed enzyme preparations with increased activity of endo-carbohydrases of depolymerase action, mutagenesis of the
Trichoderma reesei
strain was carried out using ultraviolet and ionizing irradiation. Application of fractionated UV-irradiation made it possible to increase the endoglucanase activity by 20% and xylanase activity by 80% compared to the original strain. The greatest effect was achieved after multistage gamma-irradiation mutagenesis with a cobalt source. When the mutant
T. reesei-Co-44
was cultured in shaking flasks, its endoglucanase activity was increased 5-fold, while xylanase activity increased more than 8-fold compared to the original strain. High activity of the target enzymes in the mutant strains was confirmed by the results of fed-batch cultivation in laboratory reactors with lactose feeding as a carbon source and an inducer of cellulases and xylanases biosynthesis. The maximum activity of endoglucanase and xylanase was achieved by culturing strain
T. reesei-Co-44
obtained as a result of gamma-irradiation mutagenesis and amounted to 1324.2 ± 70.1 and 3394 ± 164 U/mL, respectively. This activity level makes it possible to produce competitive enzyme preparations of carbohydrases for fodder production.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><doi>10.1134/S0026261718050120</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0026-2617 |
| ispartof | Microbiology (New York), 2018-09, Vol.87 (5), p.652-661 |
| issn | 0026-2617 1608-3237 |
| language | eng |
| recordid | cdi_proquest_journals_2116083469 |
| source | SpringerLink Journals |
| subjects | Batch culture Biomedical and Life Sciences Carbon sources Cobalt Endoglucanase Enzymes Experimental Articles Lactose Life Sciences Medical Microbiology Microbiology Mutagenesis Trichoderma reesei Ultraviolet radiation |
| title | Enhancement of Activity of Carbohydrases with Endo-depolymerase Action in Trichoderma reesei Using Mutagenesis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T13%3A42%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Enhancement%20of%20Activity%20of%20Carbohydrases%20with%20Endo-depolymerase%20Action%20in%20Trichoderma%20reesei%20Using%20Mutagenesis&rft.jtitle=Microbiology%20(New%20York)&rft.au=Kostyleva,%20E.%20V.&rft.date=2018-09-01&rft.volume=87&rft.issue=5&rft.spage=652&rft.epage=661&rft.pages=652-661&rft.issn=0026-2617&rft.eissn=1608-3237&rft_id=info:doi/10.1134/S0026261718050120&rft_dat=%3Cproquest_cross%3E2116083469%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2116083469&rft_id=info:pmid/&rfr_iscdi=true |