Phylogenetic, toxigenic and virulence profiles of Alternaria species causing leaf blight of tomato in Egypt
Species of Alternaria are serious plant pathogens, causing major losses on a wide range of crops. Leaf blight symptoms were observed on tomato leaves, and samples were collected from various regions. Isolation was done from symptomatic tomato leaves, and 15 representatives were selected from a colle...
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| Veröffentlicht in: | Mycological progress 2018-11, Vol.17 (11), p.1269-1282 |
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| creator | El Gobashy, Samah Fawzy Mikhail, Wafai Z. A. Ismail, Ahmed Mahmoud Zekry, Adel Moretti, Anotonio Susca, Antonella Soliman, Amira Sh |
| description | Species of
Alternaria
are serious plant pathogens, causing major losses on a wide range of crops. Leaf blight symptoms were observed on tomato leaves, and samples were collected from various regions. Isolation was done from symptomatic tomato leaves, and 15 representatives were selected from a collection of 65 isolates of
Alternaria
species. The virulence of
Alternaria
isolates was investigated on detached leaves (DL) and whole plants of tomato cv. Super strain B. A phylogenetic analysis was performed based on three partial gene regions, the glyceraldehyde-3-phosphate dehydrogenase (
GAPDH
), the RNA polymerase second largest subunit (
RPB2
) and the
Alternaria
major allergen gene (
Alt a 1
). The potentiality of
Alternaria
isolates to produce toxins was also investigated on the basis of thin-layer chromatography (TLC). Our investigations revealed that
Alternaria
isolates showed different levels of virulence either on tomato plants or DL. Based on the phylogeny of three genes,
Alternaria
isolates encompassed two species of small-spored morphospecies:
A
.
alternata
(14 isolates) and
A
.
arborescens
(single isolate). The produced toxins varied among
Alternaria
isolates with tenuazonic acid (TeA) being the most abundant mycotoxin produced by most isolates. This study highlighted on other
Alternaria
species in Egypt that might represent a serious concern for tomato producers as causal agents of leaf blight over other species, i.e.
A. solani
. |
| doi_str_mv | 10.1007/s11557-018-1442-1 |
| format | Article |
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Alternaria
are serious plant pathogens, causing major losses on a wide range of crops. Leaf blight symptoms were observed on tomato leaves, and samples were collected from various regions. Isolation was done from symptomatic tomato leaves, and 15 representatives were selected from a collection of 65 isolates of
Alternaria
species. The virulence of
Alternaria
isolates was investigated on detached leaves (DL) and whole plants of tomato cv. Super strain B. A phylogenetic analysis was performed based on three partial gene regions, the glyceraldehyde-3-phosphate dehydrogenase (
GAPDH
), the RNA polymerase second largest subunit (
RPB2
) and the
Alternaria
major allergen gene (
Alt a 1
). The potentiality of
Alternaria
isolates to produce toxins was also investigated on the basis of thin-layer chromatography (TLC). Our investigations revealed that
Alternaria
isolates showed different levels of virulence either on tomato plants or DL. Based on the phylogeny of three genes,
Alternaria
isolates encompassed two species of small-spored morphospecies:
A
.
alternata
(14 isolates) and
A
.
arborescens
(single isolate). The produced toxins varied among
Alternaria
isolates with tenuazonic acid (TeA) being the most abundant mycotoxin produced by most isolates. This study highlighted on other
Alternaria
species in Egypt that might represent a serious concern for tomato producers as causal agents of leaf blight over other species, i.e.
A. solani
.</description><identifier>ISSN: 1617-416X</identifier><identifier>EISSN: 1861-8952</identifier><identifier>DOI: 10.1007/s11557-018-1442-1</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Allergens ; Alternaria ; Biomedical and Life Sciences ; Blight ; DNA-directed RNA polymerase ; Ecology ; Geographical variations ; Glyceraldehyde-3-phosphate dehydrogenase ; Investigations ; Leaf blight ; Leaves ; Life Sciences ; Microbiology ; Mycotoxins ; Original Article ; Phylogenetics ; Phylogeny ; Plant Sciences ; Plants ; Ribonucleic acid ; RNA ; Species ; Tenuazonic acid ; Thin layer chromatography ; Tomatoes ; Toxins ; Virulence</subject><ispartof>Mycological progress, 2018-11, Vol.17 (11), p.1269-1282</ispartof><rights>German Mycological Society and Springer-Verlag GmbH Germany, part of Springer Nature 2018</rights><rights>Mycological Progress is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-b40f28a0c584bc91c8752d47a5afffb1d55c6e1af18aca0c11820cff2e3f2aac3</citedby><cites>FETCH-LOGICAL-c316t-b40f28a0c584bc91c8752d47a5afffb1d55c6e1af18aca0c11820cff2e3f2aac3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11557-018-1442-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11557-018-1442-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>El Gobashy, Samah Fawzy</creatorcontrib><creatorcontrib>Mikhail, Wafai Z. A.</creatorcontrib><creatorcontrib>Ismail, Ahmed Mahmoud</creatorcontrib><creatorcontrib>Zekry, Adel</creatorcontrib><creatorcontrib>Moretti, Anotonio</creatorcontrib><creatorcontrib>Susca, Antonella</creatorcontrib><creatorcontrib>Soliman, Amira Sh</creatorcontrib><title>Phylogenetic, toxigenic and virulence profiles of Alternaria species causing leaf blight of tomato in Egypt</title><title>Mycological progress</title><addtitle>Mycol Progress</addtitle><description>Species of
Alternaria
are serious plant pathogens, causing major losses on a wide range of crops. Leaf blight symptoms were observed on tomato leaves, and samples were collected from various regions. Isolation was done from symptomatic tomato leaves, and 15 representatives were selected from a collection of 65 isolates of
Alternaria
species. The virulence of
Alternaria
isolates was investigated on detached leaves (DL) and whole plants of tomato cv. Super strain B. A phylogenetic analysis was performed based on three partial gene regions, the glyceraldehyde-3-phosphate dehydrogenase (
GAPDH
), the RNA polymerase second largest subunit (
RPB2
) and the
Alternaria
major allergen gene (
Alt a 1
). The potentiality of
Alternaria
isolates to produce toxins was also investigated on the basis of thin-layer chromatography (TLC). Our investigations revealed that
Alternaria
isolates showed different levels of virulence either on tomato plants or DL. Based on the phylogeny of three genes,
Alternaria
isolates encompassed two species of small-spored morphospecies:
A
.
alternata
(14 isolates) and
A
.
arborescens
(single isolate). The produced toxins varied among
Alternaria
isolates with tenuazonic acid (TeA) being the most abundant mycotoxin produced by most isolates. This study highlighted on other
Alternaria
species in Egypt that might represent a serious concern for tomato producers as causal agents of leaf blight over other species, i.e.
A. solani
.</description><subject>Allergens</subject><subject>Alternaria</subject><subject>Biomedical and Life Sciences</subject><subject>Blight</subject><subject>DNA-directed RNA polymerase</subject><subject>Ecology</subject><subject>Geographical variations</subject><subject>Glyceraldehyde-3-phosphate dehydrogenase</subject><subject>Investigations</subject><subject>Leaf blight</subject><subject>Leaves</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Mycotoxins</subject><subject>Original Article</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Plant Sciences</subject><subject>Plants</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Species</subject><subject>Tenuazonic acid</subject><subject>Thin layer chromatography</subject><subject>Tomatoes</subject><subject>Toxins</subject><subject>Virulence</subject><issn>1617-416X</issn><issn>1861-8952</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kMtKAzEUhoMoWC8P4C7g1mhOJplJl6XUCxR0oeAuZNJkmjqdGZNU7NubMoIrV-fC9x8OH0JXQG-B0uouAghREQqSAOeMwBGagCyByKlgx7kvoSIcyvdTdBbjhlLOOS0m6ONlvW_7xnY2eXODU__t8-AN1t0Kf_mwa21nLB5C73xrI-4dnrXJhk4Hr3EcrPF5a_Qu-q7BrdUO161v1ulApn6rU499hxfNfkgX6MTpNtrL33qO3u4Xr_NHsnx-eJrPlsQUUCZSc-qY1NQIyWszBSMrwVa80kI752pYCWFKC9qB1CZjAJJR4xyzhWNam-IcXY9389efOxuT2vS7_HEbFcuapoyzssgUjJQJfYzBOjUEv9Vhr4Cqg1M1OlXZqTo4VZAzbMzEzHaNDX-X_w_9AMP5e8M</recordid><startdate>20181101</startdate><enddate>20181101</enddate><creator>El Gobashy, Samah Fawzy</creator><creator>Mikhail, Wafai Z. A.</creator><creator>Ismail, Ahmed Mahmoud</creator><creator>Zekry, Adel</creator><creator>Moretti, Anotonio</creator><creator>Susca, Antonella</creator><creator>Soliman, Amira Sh</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7N</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope></search><sort><creationdate>20181101</creationdate><title>Phylogenetic, toxigenic and virulence profiles of Alternaria species causing leaf blight of tomato in Egypt</title><author>El Gobashy, Samah Fawzy ; Mikhail, Wafai Z. A. ; Ismail, Ahmed Mahmoud ; Zekry, Adel ; Moretti, Anotonio ; Susca, Antonella ; Soliman, Amira Sh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-b40f28a0c584bc91c8752d47a5afffb1d55c6e1af18aca0c11820cff2e3f2aac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Allergens</topic><topic>Alternaria</topic><topic>Biomedical and Life Sciences</topic><topic>Blight</topic><topic>DNA-directed RNA polymerase</topic><topic>Ecology</topic><topic>Geographical variations</topic><topic>Glyceraldehyde-3-phosphate dehydrogenase</topic><topic>Investigations</topic><topic>Leaf blight</topic><topic>Leaves</topic><topic>Life Sciences</topic><topic>Microbiology</topic><topic>Mycotoxins</topic><topic>Original Article</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Plant Sciences</topic><topic>Plants</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Species</topic><topic>Tenuazonic acid</topic><topic>Thin layer chromatography</topic><topic>Tomatoes</topic><topic>Toxins</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>El Gobashy, Samah Fawzy</creatorcontrib><creatorcontrib>Mikhail, Wafai Z. A.</creatorcontrib><creatorcontrib>Ismail, Ahmed Mahmoud</creatorcontrib><creatorcontrib>Zekry, Adel</creatorcontrib><creatorcontrib>Moretti, Anotonio</creatorcontrib><creatorcontrib>Susca, Antonella</creatorcontrib><creatorcontrib>Soliman, Amira Sh</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><jtitle>Mycological progress</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>El Gobashy, Samah Fawzy</au><au>Mikhail, Wafai Z. A.</au><au>Ismail, Ahmed Mahmoud</au><au>Zekry, Adel</au><au>Moretti, Anotonio</au><au>Susca, Antonella</au><au>Soliman, Amira Sh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phylogenetic, toxigenic and virulence profiles of Alternaria species causing leaf blight of tomato in Egypt</atitle><jtitle>Mycological progress</jtitle><stitle>Mycol Progress</stitle><date>2018-11-01</date><risdate>2018</risdate><volume>17</volume><issue>11</issue><spage>1269</spage><epage>1282</epage><pages>1269-1282</pages><issn>1617-416X</issn><eissn>1861-8952</eissn><abstract>Species of
Alternaria
are serious plant pathogens, causing major losses on a wide range of crops. Leaf blight symptoms were observed on tomato leaves, and samples were collected from various regions. Isolation was done from symptomatic tomato leaves, and 15 representatives were selected from a collection of 65 isolates of
Alternaria
species. The virulence of
Alternaria
isolates was investigated on detached leaves (DL) and whole plants of tomato cv. Super strain B. A phylogenetic analysis was performed based on three partial gene regions, the glyceraldehyde-3-phosphate dehydrogenase (
GAPDH
), the RNA polymerase second largest subunit (
RPB2
) and the
Alternaria
major allergen gene (
Alt a 1
). The potentiality of
Alternaria
isolates to produce toxins was also investigated on the basis of thin-layer chromatography (TLC). Our investigations revealed that
Alternaria
isolates showed different levels of virulence either on tomato plants or DL. Based on the phylogeny of three genes,
Alternaria
isolates encompassed two species of small-spored morphospecies:
A
.
alternata
(14 isolates) and
A
.
arborescens
(single isolate). The produced toxins varied among
Alternaria
isolates with tenuazonic acid (TeA) being the most abundant mycotoxin produced by most isolates. This study highlighted on other
Alternaria
species in Egypt that might represent a serious concern for tomato producers as causal agents of leaf blight over other species, i.e.
A. solani
.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><doi>10.1007/s11557-018-1442-1</doi><tpages>14</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1617-416X |
| ispartof | Mycological progress, 2018-11, Vol.17 (11), p.1269-1282 |
| issn | 1617-416X 1861-8952 |
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| source | SpringerLink Journals - AutoHoldings |
| subjects | Allergens Alternaria Biomedical and Life Sciences Blight DNA-directed RNA polymerase Ecology Geographical variations Glyceraldehyde-3-phosphate dehydrogenase Investigations Leaf blight Leaves Life Sciences Microbiology Mycotoxins Original Article Phylogenetics Phylogeny Plant Sciences Plants Ribonucleic acid RNA Species Tenuazonic acid Thin layer chromatography Tomatoes Toxins Virulence |
| title | Phylogenetic, toxigenic and virulence profiles of Alternaria species causing leaf blight of tomato in Egypt |
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